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Protein

Osteopontin

Gene

SPP1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Biomineralization, Cell adhesion

Keywords - Ligandi

Sialic acid

Names & Taxonomyi

Protein namesi
Recommended name:
Osteopontin
Alternative name(s):
Bone sialoprotein 1
Secreted phosphoprotein 1
Short name:
SPP-1
Gene namesi
Name:SPP1
Synonyms:OPN
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 162 PublicationsAdd BLAST16
ChainiPRO_000002032017 – 278OsteopontinAdd BLAST262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23Phosphoserine1 Publication1
Modified residuei24Phosphoserine1 Publication1
Modified residuei26Phosphoserine1 Publication1
Modified residuei27Phosphoserine1 Publication1
Modified residuei60Phosphoserine1 Publication1
Modified residuei62Phosphoserine1 Publication1
Modified residuei63Phosphoserine1 Publication1
Modified residuei66PhosphothreonineBy similarity1
Modified residuei76Phosphoserine1 Publication1
Modified residuei78Phosphoserine1 Publication1
Modified residuei81Phosphoserine1 Publication1
Modified residuei95Phosphoserine1 Publication1
Modified residuei100Phosphoserine1 Publication1
Modified residuei103Phosphoserine1 Publication1
Modified residuei115Phosphoserine1 Publication1
Modified residuei118PhosphoserineBy similarity1
Modified residuei121PhosphoserineBy similarity1
Modified residuei124Phosphoserine1 Publication1
Glycosylationi131O-linked (GalNAc...)1
Glycosylationi140O-linked (GalNAc...)1
Glycosylationi145O-linked (GalNAc...)1
Modified residuei178Phosphothreonine1 Publication1
Modified residuei183PhosphothreonineBy similarity1
Modified residuei184Phosphoserine1 Publication1
Modified residuei188Phosphoserine1 Publication1
Modified residuei199PhosphoserineBy similarity1
Modified residuei205Phosphoserine1 Publication1
Modified residuei208PhosphothreonineBy similarity1
Modified residuei210Phosphoserine1 Publication1
Modified residuei214PhosphoserineBy similarity1
Modified residuei228PhosphoserineBy similarity1
Modified residuei233Phosphoserine1 Publication1
Modified residuei237PhosphoserineBy similarity1
Modified residuei240Phosphoserine1 Publication1
Modified residuei245Phosphoserine1 Publication1
Modified residuei256Phosphoserine1 Publication1
Modified residuei267Phosphoserine1 Publication1
Modified residuei272Phosphoserine1 Publication1
Modified residuei274Phosphoserine1 Publication1
Modified residuei275PhosphoserineBy similarity1

Post-translational modificationi

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.By similarity
N- and O-glycosylated.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei79Not glycosylated1
Sitei101Not glycosylated1
Sitei209Not glycosylated1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP31096.
PeptideAtlasiP31096.
PRIDEiP31096.

PTM databases

iPTMnetiP31096.

Expressioni

Tissue specificityi

Bone, inner ear, kidney, uterus, lung, brain, epidermis.

Interactioni

Subunit structurei

Ligand for integrin alpha-V/beta-3.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000044782.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi152 – 154Cell attachment site3

Sequence similaritiesi

Belongs to the osteopontin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVP1. Eukaryota.
ENOG41116B5. LUCA.
HOGENOMiHOG000059656.
HOVERGENiHBG001731.
InParanoidiP31096.
KOiK06250.

Family and domain databases

InterProiIPR002038. Osteopontin.
IPR019841. Osteopontin_CS.
[Graphical view]
PANTHERiPTHR10607. PTHR10607. 1 hit.
PfamiPF00865. Osteopontin. 2 hits.
[Graphical view]
PRINTSiPR00216. OSTEOPONTIN.
SMARTiSM00017. OSTEO. 1 hit.
[Graphical view]
PROSITEiPS00884. OSTEOPONTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIAVICFCL LGIASALPVK PTSSGSSEEK QLNNKYPDAV ATWLKPDPSQ
60 70 80 90 100
KQTFLAPQNS VSSEETDDNK QNTLPSKSNE SPEQTDDLDD DDDNSQDVNS
110 120 130 140 150
NDSDDAETTD DPDHSDESHH SDESDEVDFP TDIPTIAVFT PFIPTESAND
160 170 180 190 200
GRGDSVAYGL KSRSKKFRRS NVQSPDATEE DFTSHIESEE MHDAPKKTSQ
210 220 230 240 250
LTDHSKETNS SELSKELTPK AKDKNKHSNL IESQENSKLS QEFHSLEDKL
260 270
DLDHKSEEDK HLKIRISHEL DSASSEVN
Length:278
Mass (Da):30,904
Last modified:June 27, 2006 - v2
Checksum:i8170204507864E87
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27S → K AA sequence (PubMed:8663267).Curated1
Sequence conflicti42T → I in AAA30462 (PubMed:1721033).Curated1
Sequence conflicti56A → T in AAA30462 (PubMed:1721033).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M66236 mRNA. Translation: AAA30462.1.
AF492837 mRNA. Translation: AAL99081.1.
AY878328 Genomic DNA. Translation: AAX62809.1.
AJ871176 Genomic DNA. Translation: CAI38798.1.
PIRiJS0638.
RefSeqiNP_776612.1. NM_174187.2.
XP_005207799.1. XM_005207742.1.
UniGeneiBt.10536.

Genome annotation databases

GeneIDi281499.
KEGGibta:281499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M66236 mRNA. Translation: AAA30462.1.
AF492837 mRNA. Translation: AAL99081.1.
AY878328 Genomic DNA. Translation: AAX62809.1.
AJ871176 Genomic DNA. Translation: CAI38798.1.
PIRiJS0638.
RefSeqiNP_776612.1. NM_174187.2.
XP_005207799.1. XM_005207742.1.
UniGeneiBt.10536.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000044782.

PTM databases

iPTMnetiP31096.

Proteomic databases

PaxDbiP31096.
PeptideAtlasiP31096.
PRIDEiP31096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281499.
KEGGibta:281499.

Organism-specific databases

CTDi6696.

Phylogenomic databases

eggNOGiENOG410IVP1. Eukaryota.
ENOG41116B5. LUCA.
HOGENOMiHOG000059656.
HOVERGENiHBG001731.
InParanoidiP31096.
KOiK06250.

Family and domain databases

InterProiIPR002038. Osteopontin.
IPR019841. Osteopontin_CS.
[Graphical view]
PANTHERiPTHR10607. PTHR10607. 1 hit.
PfamiPF00865. Osteopontin. 2 hits.
[Graphical view]
PRINTSiPR00216. OSTEOPONTIN.
SMARTiSM00017. OSTEO. 1 hit.
[Graphical view]
PROSITEiPS00884. OSTEOPONTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOSTP_BOVIN
AccessioniPrimary (citable) accession number: P31096
Secondary accession number(s): Q56NM9, Q8SPS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: June 27, 2006
Last modified: October 5, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.