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Protein

Osteopontin

Gene

SPP1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Biomineralization, Cell adhesion

Keywords - Ligandi

Sialic acid

Names & Taxonomyi

Protein namesi
Recommended name:
Osteopontin
Alternative name(s):
Bone sialoprotein 1
Secreted phosphoprotein 1
Short name:
SPP-1
Gene namesi
Name:SPP1
Synonyms:OPN
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16162 PublicationsAdd
BLAST
Chaini17 – 278262OsteopontinPRO_0000020320Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231Phosphoserine1 Publication
Modified residuei24 – 241Phosphoserine1 Publication
Modified residuei26 – 261Phosphoserine1 Publication
Modified residuei27 – 271Phosphoserine1 Publication
Modified residuei60 – 601Phosphoserine1 Publication
Modified residuei62 – 621Phosphoserine1 Publication
Modified residuei63 – 631Phosphoserine1 Publication
Modified residuei66 – 661PhosphothreonineBy similarity
Modified residuei76 – 761Phosphoserine1 Publication
Modified residuei78 – 781Phosphoserine1 Publication
Modified residuei81 – 811Phosphoserine1 Publication
Modified residuei95 – 951Phosphoserine1 Publication
Modified residuei100 – 1001Phosphoserine1 Publication
Modified residuei103 – 1031Phosphoserine1 Publication
Modified residuei115 – 1151Phosphoserine1 Publication
Modified residuei118 – 1181PhosphoserineBy similarity
Modified residuei121 – 1211PhosphoserineBy similarity
Modified residuei124 – 1241Phosphoserine1 Publication
Glycosylationi131 – 1311O-linked (GalNAc...)
Glycosylationi140 – 1401O-linked (GalNAc...)
Glycosylationi145 – 1451O-linked (GalNAc...)
Modified residuei178 – 1781Phosphothreonine1 Publication
Modified residuei183 – 1831PhosphothreonineBy similarity
Modified residuei184 – 1841Phosphoserine1 Publication
Modified residuei188 – 1881Phosphoserine1 Publication
Modified residuei199 – 1991PhosphoserineBy similarity
Modified residuei205 – 2051Phosphoserine1 Publication
Modified residuei208 – 2081PhosphothreonineBy similarity
Modified residuei210 – 2101Phosphoserine1 Publication
Modified residuei214 – 2141PhosphoserineBy similarity
Modified residuei228 – 2281PhosphoserineBy similarity
Modified residuei233 – 2331Phosphoserine1 Publication
Modified residuei237 – 2371PhosphoserineBy similarity
Modified residuei240 – 2401Phosphoserine1 Publication
Modified residuei245 – 2451Phosphoserine1 Publication
Modified residuei256 – 2561Phosphoserine1 Publication
Modified residuei267 – 2671Phosphoserine1 Publication
Modified residuei272 – 2721Phosphoserine1 Publication
Modified residuei274 – 2741Phosphoserine1 Publication
Modified residuei275 – 2751PhosphoserineBy similarity

Post-translational modificationi

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.By similarity
N- and O-glycosylated.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei79 – 791Not glycosylated
Sitei101 – 1011Not glycosylated
Sitei209 – 2091Not glycosylated

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP31096.

PTM databases

iPTMnetiP31096.

Expressioni

Tissue specificityi

Bone, inner ear, kidney, uterus, lung, brain, epidermis.

Interactioni

Subunit structurei

Ligand for integrin alpha-V/beta-3.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000044782.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi152 – 1543Cell attachment site

Sequence similaritiesi

Belongs to the osteopontin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVP1. Eukaryota.
ENOG41116B5. LUCA.
HOGENOMiHOG000059656.
HOVERGENiHBG001731.
InParanoidiP31096.
KOiK06250.

Family and domain databases

InterProiIPR002038. Osteopontin.
IPR019841. Osteopontin_CS.
[Graphical view]
PANTHERiPTHR10607. PTHR10607. 1 hit.
PfamiPF00865. Osteopontin. 2 hits.
[Graphical view]
PRINTSiPR00216. OSTEOPONTIN.
SMARTiSM00017. OSTEO. 1 hit.
[Graphical view]
PROSITEiPS00884. OSTEOPONTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIAVICFCL LGIASALPVK PTSSGSSEEK QLNNKYPDAV ATWLKPDPSQ
60 70 80 90 100
KQTFLAPQNS VSSEETDDNK QNTLPSKSNE SPEQTDDLDD DDDNSQDVNS
110 120 130 140 150
NDSDDAETTD DPDHSDESHH SDESDEVDFP TDIPTIAVFT PFIPTESAND
160 170 180 190 200
GRGDSVAYGL KSRSKKFRRS NVQSPDATEE DFTSHIESEE MHDAPKKTSQ
210 220 230 240 250
LTDHSKETNS SELSKELTPK AKDKNKHSNL IESQENSKLS QEFHSLEDKL
260 270
DLDHKSEEDK HLKIRISHEL DSASSEVN
Length:278
Mass (Da):30,904
Last modified:June 27, 2006 - v2
Checksum:i8170204507864E87
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271S → K AA sequence (PubMed:8663267).Curated
Sequence conflicti42 – 421T → I in AAA30462 (PubMed:1721033).Curated
Sequence conflicti56 – 561A → T in AAA30462 (PubMed:1721033).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M66236 mRNA. Translation: AAA30462.1.
AF492837 mRNA. Translation: AAL99081.1.
AY878328 Genomic DNA. Translation: AAX62809.1.
AJ871176 Genomic DNA. Translation: CAI38798.1.
PIRiJS0638.
RefSeqiNP_776612.1. NM_174187.2.
XP_005207799.1. XM_005207742.1.
UniGeneiBt.10536.

Genome annotation databases

GeneIDi281499.
KEGGibta:281499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M66236 mRNA. Translation: AAA30462.1.
AF492837 mRNA. Translation: AAL99081.1.
AY878328 Genomic DNA. Translation: AAX62809.1.
AJ871176 Genomic DNA. Translation: CAI38798.1.
PIRiJS0638.
RefSeqiNP_776612.1. NM_174187.2.
XP_005207799.1. XM_005207742.1.
UniGeneiBt.10536.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000044782.

PTM databases

iPTMnetiP31096.

Proteomic databases

PaxDbiP31096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281499.
KEGGibta:281499.

Organism-specific databases

CTDi6696.

Phylogenomic databases

eggNOGiENOG410IVP1. Eukaryota.
ENOG41116B5. LUCA.
HOGENOMiHOG000059656.
HOVERGENiHBG001731.
InParanoidiP31096.
KOiK06250.

Miscellaneous databases

NextBioi20805471.

Family and domain databases

InterProiIPR002038. Osteopontin.
IPR019841. Osteopontin_CS.
[Graphical view]
PANTHERiPTHR10607. PTHR10607. 1 hit.
PfamiPF00865. Osteopontin. 2 hits.
[Graphical view]
PRINTSiPR00216. OSTEOPONTIN.
SMARTiSM00017. OSTEO. 1 hit.
[Graphical view]
PROSITEiPS00884. OSTEOPONTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The cDNA cloning and RNA distribution of bovine osteopontin."
    Kerr J.M., Fisher L.W., Termine J.D., Young M.F.
    Gene 108:237-243(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of osteopontin (OPN) in Korean native cattle."
    Lee T.Y., Ju S.K., Nam M.S.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Korean.
    Tissue: Kidney.
  3. "Fine-mapping milk production quantitative trait loci on BTA6: Analysis of the bovine osteopontin gene."
    Schnabel R.D., Kim J.J., Ashwell M.S., Sonstegard T.S., Van Tassell C.P., Connor E.E., Taylor J.F.
    Proc. Natl. Acad. Sci. U.S.A. 102:6896-6901(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Identification of a missense mutation in the bovine ABCG2 gene with a major effect on the QTL on chromosome 6 affecting milk yield and composition in Holstein cattle."
    Cohen-Zinder M., Seroussi E., Larkin D.M., Loor J.J., Everts-van der Wind A., Lee J.-H., Drackley J.K., Band M.R., Hernandez A.G., Shani M., Lewin H.A., Weller J.I., Ron M.
    Genome Res. 15:936-944(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Holstein.
  5. "Purification and characterization of three proteins isolated from the proteose peptone fraction of bovine milk."
    Soerensen E.S., Petersen T.E.
    J. Dairy Res. 60:189-197(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-22; 44-54; 168-182 AND 221-243.
    Tissue: Milk.
  6. "Phosphorylation of purified bovine bone sialoprotein and osteopontin by protein kinases."
    Salih E., Zhou H.-Y., Glimcher M.J.
    J. Biol. Chem. 271:16897-16905(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-28, PHOSPHORYLATION.
  7. "Posttranslational modifications of bovine osteopontin: identification of twenty-eight phosphorylation and three O-glycosylation sites."
    Soerensen E.S., Hoejrup P., Petersen T.E.
    Protein Sci. 4:2040-2049(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-23; SER-24; SER-26; SER-60; SER-62; SER-63; SER-; SER-76; SER-81; SER-95; SER-100; SER-103; SER-115; SER-124; THR-178; SER-184; SER-188; SER-205; SER-210; SER-233; SER-240; SER-245; SER-256; SER-267; SER-272 AND SER-274.
    Tissue: Milk.

Entry informationi

Entry nameiOSTP_BOVIN
AccessioniPrimary (citable) accession number: P31096
Secondary accession number(s): Q56NM9, Q8SPS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: June 27, 2006
Last modified: January 20, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.