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P31074

- DYR_ENTAE

UniProt

P31074 - DYR_ENTAE

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Protein
Dihydrofolate reductase
Gene
folA, atsR
Organism
Enterobacter aerogenes (Aerobacter aerogenes)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei5 – 51Substrate; via carbonyl oxygen By similarity
Binding sitei7 – 71NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei27 – 271Substrate By similarity
Binding sitei52 – 521Substrate By similarity
Binding sitei57 – 571Substrate By similarity
Binding sitei76 – 761NADP; via carbonyl oxygen By similarity
Binding sitei113 – 1131Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 197NADP By similarity
Nucleotide bindingi45 – 462NADP By similarity
Nucleotide bindingi63 – 642NADP By similarity
Nucleotide bindingi95 – 1028NADP By similarity

GO - Molecular functioni

  1. NADP binding Source: InterPro
  2. dihydrofolate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:atsR
OrganismiEnterobacter aerogenes (Aerobacter aerogenes)
Taxonomic identifieri548 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 159159Dihydrofolate reductase
PRO_0000186389Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP31074.
SMRiP31074. Positions 1-159.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 158158DHFR
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31074-1 [UniParc]FASTAAdd to Basket

« Hide

MISLIAALAV DRVIGMENAM PWDLPADLAW FKRNTLNKPV VMGRLTWESI    50
GRPLPGRKNI VISSKPGSDD RVQWVKSVDE AIAACGDAEE IMVIGGGRVY 100
EQFLPKAHKL YLTHIDAEVE GDTHFPDYDP DEWESVFSEF HDADAQNSHS 150
YCFEILERR 159
Length:159
Mass (Da):17,974
Last modified:July 1, 1993 - v1
Checksum:i73C9B8D31D0C1C07
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91A → R in BAA01187. 1 Publication
Sequence conflicti23 – 264DLPA → NLNE in BAA01187. 1 Publication
Sequence conflicti76 – 761K → S in BAA01187. 1 Publication
Sequence conflicti79 – 791D → E in BAA01187. 1 Publication
Sequence conflicti88 – 881A → V in BAA01187. 1 Publication
Sequence conflicti101 – 1022EQ → DE in BAA01187. 1 Publication
Sequence conflicti108 – 1081H → Q in BAA01187. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26022 Genomic DNA. Translation: AAA24800.1.
D10358 Genomic DNA. Translation: BAA01187.1.
PIRiA39799.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26022 Genomic DNA. Translation: AAA24800.1 .
D10358 Genomic DNA. Translation: BAA01187.1 .
PIRi A39799.

3D structure databases

ProteinModelPortali P31074.
SMRi P31074. Positions 1-159.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL3853.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .

Family and domain databases

Gene3Di 3.40.430.10. 1 hit.
InterProi IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000194. DHFR. 1 hit.
PRINTSi PR00070. DHFR.
SUPFAMi SSF53597. SSF53597. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Temporal and topological clustering of diverged residues among enterobacterial dihydrofolate reductases."
    Garvin L.D., Hardies S.C.
    Mol. Biol. Evol. 8:654-668(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and nucleotide sequence of a negative regulator gene for Klebsiella aerogenes arylsulfatase synthesis and identification of the gene as folA."
    Azakami H., Sugino H., Murooka Y.
    J. Bacteriol. 174:2344-2351(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: W70.

Entry informationi

Entry nameiDYR_ENTAE
AccessioniPrimary (citable) accession number: P31074
Secondary accession number(s): P27498
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: December 11, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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