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Protein

Dihydrofolate reductase

Gene

folA

Organism
Enterobacter aerogenes (Aerobacter aerogenes)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei5 – 51Substrate; via carbonyl oxygenBy similarity
Binding sitei7 – 71NADP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei27 – 271SubstrateBy similarity
Binding sitei52 – 521SubstrateBy similarity
Binding sitei57 – 571SubstrateBy similarity
Binding sitei76 – 761NADP; via carbonyl oxygenBy similarity
Binding sitei113 – 1131SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 197NADPBy similarity
Nucleotide bindingi45 – 462NADPBy similarity
Nucleotide bindingi63 – 642NADPBy similarity
Nucleotide bindingi95 – 1028NADPBy similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:atsR
OrganismiEnterobacter aerogenes (Aerobacter aerogenes)
Taxonomic identifieri548 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 159159Dihydrofolate reductasePRO_0000186389Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP31074.
SMRiP31074. Positions 1-159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 158158DHFRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31074-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MISLIAALAV DRVIGMENAM PWDLPADLAW FKRNTLNKPV VMGRLTWESI
60 70 80 90 100
GRPLPGRKNI VISSKPGSDD RVQWVKSVDE AIAACGDAEE IMVIGGGRVY
110 120 130 140 150
EQFLPKAHKL YLTHIDAEVE GDTHFPDYDP DEWESVFSEF HDADAQNSHS

YCFEILERR
Length:159
Mass (Da):17,974
Last modified:July 1, 1993 - v1
Checksum:i73C9B8D31D0C1C07
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91A → R in BAA01187. (PubMed:1551851)Curated
Sequence conflicti23 – 264DLPA → NLNE in BAA01187. (PubMed:1551851)Curated
Sequence conflicti76 – 761K → S in BAA01187. (PubMed:1551851)Curated
Sequence conflicti79 – 791D → E in BAA01187. (PubMed:1551851)Curated
Sequence conflicti88 – 881A → V in BAA01187. (PubMed:1551851)Curated
Sequence conflicti101 – 1022EQ → DE in BAA01187. (PubMed:1551851)Curated
Sequence conflicti108 – 1081H → Q in BAA01187. (PubMed:1551851)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26022 Genomic DNA. Translation: AAA24800.1.
D10358 Genomic DNA. Translation: BAA01187.1.
PIRiA39799.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26022 Genomic DNA. Translation: AAA24800.1.
D10358 Genomic DNA. Translation: BAA01187.1.
PIRiA39799.

3D structure databases

ProteinModelPortaliP31074.
SMRiP31074. Positions 1-159.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL3853.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Temporal and topological clustering of diverged residues among enterobacterial dihydrofolate reductases."
    Garvin L.D., Hardies S.C.
    Mol. Biol. Evol. 8:654-668(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and nucleotide sequence of a negative regulator gene for Klebsiella aerogenes arylsulfatase synthesis and identification of the gene as folA."
    Azakami H., Sugino H., Murooka Y.
    J. Bacteriol. 174:2344-2351(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: W70.

Entry informationi

Entry nameiDYR_ENTAE
AccessioniPrimary (citable) accession number: P31074
Secondary accession number(s): P27498
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 1, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.