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P31074

- DYR_ENTAE

UniProt

P31074 - DYR_ENTAE

Protein

Dihydrofolate reductase

Gene

folA

Organism
Enterobacter aerogenes (Aerobacter aerogenes)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei5 – 51Substrate; via carbonyl oxygenBy similarity
    Binding sitei7 – 71NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei27 – 271SubstrateBy similarity
    Binding sitei52 – 521SubstrateBy similarity
    Binding sitei57 – 571SubstrateBy similarity
    Binding sitei76 – 761NADP; via carbonyl oxygenBy similarity
    Binding sitei113 – 1131SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi13 – 197NADPBy similarity
    Nucleotide bindingi45 – 462NADPBy similarity
    Nucleotide bindingi63 – 642NADPBy similarity
    Nucleotide bindingi95 – 1028NADPBy similarity

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. glycine biosynthetic process Source: InterPro
    2. nucleotide biosynthetic process Source: InterPro
    3. one-carbon metabolic process Source: UniProtKB-KW
    4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrofolate reductase (EC:1.5.1.3)
    Gene namesi
    Name:folA
    Synonyms:atsR
    OrganismiEnterobacter aerogenes (Aerobacter aerogenes)
    Taxonomic identifieri548 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 159159Dihydrofolate reductasePRO_0000186389Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP31074.
    SMRiP31074. Positions 1-159.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 158158DHFRPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the dihydrofolate reductase family.Curated
    Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.430.10. 1 hit.
    InterProiIPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000194. DHFR. 1 hit.
    PRINTSiPR00070. DHFR.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31074-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISLIAALAV DRVIGMENAM PWDLPADLAW FKRNTLNKPV VMGRLTWESI    50
    GRPLPGRKNI VISSKPGSDD RVQWVKSVDE AIAACGDAEE IMVIGGGRVY 100
    EQFLPKAHKL YLTHIDAEVE GDTHFPDYDP DEWESVFSEF HDADAQNSHS 150
    YCFEILERR 159
    Length:159
    Mass (Da):17,974
    Last modified:July 1, 1993 - v1
    Checksum:i73C9B8D31D0C1C07
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 91A → R in BAA01187. (PubMed:1551851)Curated
    Sequence conflicti23 – 264DLPA → NLNE in BAA01187. (PubMed:1551851)Curated
    Sequence conflicti76 – 761K → S in BAA01187. (PubMed:1551851)Curated
    Sequence conflicti79 – 791D → E in BAA01187. (PubMed:1551851)Curated
    Sequence conflicti88 – 881A → V in BAA01187. (PubMed:1551851)Curated
    Sequence conflicti101 – 1022EQ → DE in BAA01187. (PubMed:1551851)Curated
    Sequence conflicti108 – 1081H → Q in BAA01187. (PubMed:1551851)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26022 Genomic DNA. Translation: AAA24800.1.
    D10358 Genomic DNA. Translation: BAA01187.1.
    PIRiA39799.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26022 Genomic DNA. Translation: AAA24800.1 .
    D10358 Genomic DNA. Translation: BAA01187.1 .
    PIRi A39799.

    3D structure databases

    ProteinModelPortali P31074.
    SMRi P31074. Positions 1-159.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL3853.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .

    Family and domain databases

    Gene3Di 3.40.430.10. 1 hit.
    InterProi IPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000194. DHFR. 1 hit.
    PRINTSi PR00070. DHFR.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Temporal and topological clustering of diverged residues among enterobacterial dihydrofolate reductases."
      Garvin L.D., Hardies S.C.
      Mol. Biol. Evol. 8:654-668(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cloning and nucleotide sequence of a negative regulator gene for Klebsiella aerogenes arylsulfatase synthesis and identification of the gene as folA."
      Azakami H., Sugino H., Murooka Y.
      J. Bacteriol. 174:2344-2351(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: W70.

    Entry informationi

    Entry nameiDYR_ENTAE
    AccessioniPrimary (citable) accession number: P31074
    Secondary accession number(s): P27498
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3