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P31073

- DYR_CITFR

UniProt

P31073 - DYR_CITFR

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Protein
Dihydrofolate reductase
Gene
folA
Organism
Citrobacter freundii
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei5 – 51Substrate; via carbonyl oxygen By similarity
Binding sitei7 – 71NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei27 – 271Substrate By similarity
Binding sitei52 – 521Substrate By similarity
Binding sitei57 – 571Substrate By similarity
Binding sitei76 – 761NADP; via carbonyl oxygen By similarity
Binding sitei113 – 1131Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 197NADP By similarity
Nucleotide bindingi45 – 462NADP By similarity
Nucleotide bindingi63 – 642NADP By similarity
Nucleotide bindingi95 – 1028NADP By similarity

GO - Molecular functioni

  1. NADP binding Source: InterPro
  2. dihydrofolate reductase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
OrganismiCitrobacter freundii
Taxonomic identifieri546 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 159159Dihydrofolate reductase
PRO_0000186386Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP31073.
SMRiP31073. Positions 1-159.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 158158DHFR
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31073-1 [UniParc]FASTAAdd to Basket

« Hide

MISLIAALAV DRVIGMENAM PWNLPADLAW FKRNTLNKPV VMGRHTWESI    50
GRPLPGRKNI VISSKPGTDD RVQWVKSVDE AIAACGDEPE IMVIGGGRVY 100
EQFLPKAQKL YLTHIDAEVE GDTHFPDYEP DDWESVFSEF HDADAQNSHS 150
YCFEILERR 159
Length:159
Mass (Da):18,028
Last modified:July 1, 1993 - v1
Checksum:i3CE7D7688577DCEE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26023 Genomic DNA. Translation: AAA23093.1.
PIRiB39799.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26023 Genomic DNA. Translation: AAA23093.1 .
PIRi B39799.

3D structure databases

ProteinModelPortali P31073.
SMRi P31073. Positions 1-159.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .

Family and domain databases

Gene3Di 3.40.430.10. 1 hit.
InterProi IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000194. DHFR. 1 hit.
PRINTSi PR00070. DHFR.
SUPFAMi SSF53597. SSF53597. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Temporal and topological clustering of diverged residues among enterobacterial dihydrofolate reductases."
    Garvin L.D., Hardies S.C.
    Mol. Biol. Evol. 8:654-668(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiDYR_CITFR
AccessioniPrimary (citable) accession number: P31073
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 16, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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