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P31072

- 6PGD_TRYBB

UniProt

P31072 - 6PGD_TRYBB

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Protein

6-phosphogluconate dehydrogenase, decarboxylating

Gene

GND

Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH.By similarity

Catalytic activityi

6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051NADPBy similarity
Binding sitei105 – 1051SubstrateBy similarity
Active sitei186 – 1861Proton acceptorBy similarity
Active sitei193 – 1931Proton donorBy similarity
Binding sitei194 – 1941SubstrateBy similarity
Binding sitei263 – 2631Substrate; via amide nitrogenBy similarity
Binding sitei290 – 2901SubstrateBy similarity
Binding sitei454 – 4541Substrate; shared with dimeric partnerBy similarity
Binding sitei460 – 4601Substrate; shared with dimeric partnerBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146NADPBy similarity
Nucleotide bindingi32 – 343NADPBy similarity
Nucleotide bindingi77 – 793NADPBy similarity

GO - Molecular functioni

  1. NADP binding Source: InterPro
  2. phosphogluconate dehydrogenase (decarboxylating) activity Source: UniProtKB-EC

GO - Biological processi

  1. D-gluconate metabolic process Source: UniProtKB-KW
  2. pentose-phosphate shunt Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Gluconate utilization, Pentose shunt

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00410.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphogluconate dehydrogenase, decarboxylating (EC:1.1.1.44)
Gene namesi
Name:GND
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4794796-phosphogluconate dehydrogenase, decarboxylatingPRO_0000090071Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
479
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi12 – 2312Combined sources
Beta strandi28 – 314Combined sources
Helixi35 – 4410Combined sources
Turni45 – 473Combined sources
Helixi51 – 533Combined sources
Beta strandi54 – 563Combined sources
Helixi60 – 667Combined sources
Beta strandi72 – 754Combined sources
Helixi81 – 9313Combined sources
Beta strandi99 – 1024Combined sources
Helixi108 – 11912Combined sources
Turni120 – 1223Combined sources
Beta strandi124 – 1329Combined sources
Helixi133 – 1397Combined sources
Beta strandi142 – 1476Combined sources
Helixi149 – 16214Combined sources
Helixi181 – 20929Combined sources
Helixi214 – 22613Combined sources
Helixi233 – 24311Combined sources
Beta strandi249 – 2513Combined sources
Helixi252 – 2554Combined sources
Helixi265 – 27612Combined sources
Helixi281 – 29414Combined sources
Helixi296 – 30510Combined sources
Turni307 – 3104Combined sources
Helixi325 – 35632Combined sources
Helixi362 – 3676Combined sources
Beta strandi370 – 3734Combined sources
Helixi380 – 38910Combined sources
Helixi398 – 4003Combined sources
Helixi401 – 42121Combined sources
Helixi427 – 43913Combined sources
Helixi446 – 45813Combined sources
Beta strandi462 – 47110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PGJX-ray2.82A/B2-479[»]
ProteinModelPortaliP31072.
SMRiP31072. Positions 2-479.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31072.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni131 – 1333Substrate bindingBy similarity
Regioni189 – 1902Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
1.20.5.320. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_decarbox.
IPR006115. 6PGDH_NADP-bd.
IPR006184. 6PGdom_BS.
IPR013328. DH_multihelical.
IPR012284. Fibritin/6PGD_C-extension.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000109. 6PGD. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00873. gnd. 1 hit.
PROSITEiPS00461. 6PGD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31072-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSMDVGVVGL GVMGANLALN IAEKGFKVAV FNRTYSKSEE FMKANASAPF
60 70 80 90 100
AGNLKAFETM EAFAASLKKP RKALILVQAG AATDSTTEQL KKVFEKGDIL
110 120 130 140 150
VDTGNAHFKD QGRRAQQLEA AGLRFLGMGI SGGEEGARKG PAFFPGGTLS
160 170 180 190 200
VWEEIRPIVE AAAAKADDGR PCVTMNGSGG AGSCVKMYHN SGEYAILQIW
210 220 230 240 250
GEVFDILRAM GLNNDEVAAV LEDWKSKNFL KSYMLDISIA AARAKDKDGS
260 270 280 290 300
YLTEHVMDRI GSKGTGLWSA QEALEIGVPA PSLNMAVVSR QFTMYKTERQ
310 320 330 340 350
ANASNAPGIT QSPGYTLKNK SPSGPEIKQL YDSVCIAIIS CYAQMFQCLR
360 370 380 390 400
EMDKVHNFGL NLPATIATFR AGCILQGYLL KPMTEAFEKN PNISNLMCAF
410 420 430 440 450
QTEIRAGLQN YRDMVALITS KLEVSIPVLS ASLNYVTAMF TPTLKYGQLV
460 470
SLQRDVFGRH GYERVDKDGR ESFQWPELQ
Length:479
Mass (Da):52,154
Last modified:July 1, 1993 - v1
Checksum:i64FED260915ABC2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65623 Genomic DNA. Translation: CAA46577.1.
PIRiA48565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65623 Genomic DNA. Translation: CAA46577.1 .
PIRi A48565.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PGJ X-ray 2.82 A/B 2-479 [» ]
ProteinModelPortali P31072.
SMRi P31072. Positions 2-479.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00115 ; UER00410 .

Miscellaneous databases

EvolutionaryTracei P31072.

Family and domain databases

Gene3Di 1.10.1040.10. 1 hit.
1.20.5.320. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_decarbox.
IPR006115. 6PGDH_NADP-bd.
IPR006184. 6PGdom_BS.
IPR013328. DH_multihelical.
IPR012284. Fibritin/6PGD_C-extension.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000109. 6PGD. 1 hit.
SUPFAMi SSF48179. SSF48179. 1 hit.
TIGRFAMsi TIGR00873. gnd. 1 hit.
PROSITEi PS00461. 6PGD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A 6-phosphogluconate dehydrogenase gene from Trypanosoma brucei."
    Barrett M.P., le Page R.W.F.
    Mol. Biochem. Parasitol. 57:89-100(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 427.
  2. "A 2.8-A resolution structure of 6-phosphogluconate dehydrogenase from the protozoan parasite Trypanosoma brucei: comparison with the sheep enzyme accounts for differences in activity with coenzyme and substrate analogues."
    Phillips C., Dohnalek J., Gover S., Barrett M.P., Adams M.J.
    J. Mol. Biol. 282:667-681(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), SUBUNIT.

Entry informationi

Entry namei6PGD_TRYBB
AccessioniPrimary (citable) accession number: P31072
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 26, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3