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P31072

- 6PGD_TRYBB

UniProt

P31072 - 6PGD_TRYBB

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Protein
6-phosphogluconate dehydrogenase, decarboxylating
Gene
GND
Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH By similarity.

Catalytic activityi

6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051NADP By similarity
Binding sitei105 – 1051Substrate By similarity
Active sitei186 – 1861Proton acceptor By similarity
Active sitei193 – 1931Proton donor By similarity
Binding sitei194 – 1941Substrate By similarity
Binding sitei263 – 2631Substrate; via amide nitrogen By similarity
Binding sitei290 – 2901Substrate By similarity
Binding sitei454 – 4541Substrate; shared with dimeric partner By similarity
Binding sitei460 – 4601Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146NADP By similarity
Nucleotide bindingi32 – 343NADP By similarity
Nucleotide bindingi77 – 793NADP By similarity

GO - Molecular functioni

  1. NADP binding Source: InterPro
  2. phosphogluconate dehydrogenase (decarboxylating) activity Source: UniProtKB-EC

GO - Biological processi

  1. D-gluconate metabolic process Source: UniProtKB-KW
  2. pentose-phosphate shunt Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Gluconate utilization, Pentose shunt

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00410.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphogluconate dehydrogenase, decarboxylating (EC:1.1.1.44)
Gene namesi
Name:GND
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4794796-phosphogluconate dehydrogenase, decarboxylating
PRO_0000090071Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86
Helixi12 – 2312
Beta strandi28 – 314
Helixi35 – 4410
Turni45 – 473
Helixi51 – 533
Beta strandi54 – 563
Helixi60 – 667
Beta strandi72 – 754
Helixi81 – 9313
Beta strandi99 – 1024
Helixi108 – 11912
Turni120 – 1223
Beta strandi124 – 1329
Helixi133 – 1397
Beta strandi142 – 1476
Helixi149 – 16214
Helixi181 – 20929
Helixi214 – 22613
Helixi233 – 24311
Beta strandi249 – 2513
Helixi252 – 2554
Helixi265 – 27612
Helixi281 – 29414
Helixi296 – 30510
Turni307 – 3104
Helixi325 – 35632
Helixi362 – 3676
Beta strandi370 – 3734
Helixi380 – 38910
Helixi398 – 4003
Helixi401 – 42121
Helixi427 – 43913
Helixi446 – 45813
Beta strandi462 – 47110

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PGJX-ray2.82A/B2-479[»]
ProteinModelPortaliP31072.
SMRiP31072. Positions 2-479.

Miscellaneous databases

EvolutionaryTraceiP31072.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni131 – 1333Substrate binding By similarity
Regioni189 – 1902Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
1.20.5.320. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_decarbox.
IPR006115. 6PGDH_NADP-bd.
IPR006184. 6PGdom_BS.
IPR013328. DH_multihelical.
IPR012284. Fibritin/6PGD_C-extension.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000109. 6PGD. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00873. gnd. 1 hit.
PROSITEiPS00461. 6PGD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31072-1 [UniParc]FASTAAdd to Basket

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MSMDVGVVGL GVMGANLALN IAEKGFKVAV FNRTYSKSEE FMKANASAPF    50
AGNLKAFETM EAFAASLKKP RKALILVQAG AATDSTTEQL KKVFEKGDIL 100
VDTGNAHFKD QGRRAQQLEA AGLRFLGMGI SGGEEGARKG PAFFPGGTLS 150
VWEEIRPIVE AAAAKADDGR PCVTMNGSGG AGSCVKMYHN SGEYAILQIW 200
GEVFDILRAM GLNNDEVAAV LEDWKSKNFL KSYMLDISIA AARAKDKDGS 250
YLTEHVMDRI GSKGTGLWSA QEALEIGVPA PSLNMAVVSR QFTMYKTERQ 300
ANASNAPGIT QSPGYTLKNK SPSGPEIKQL YDSVCIAIIS CYAQMFQCLR 350
EMDKVHNFGL NLPATIATFR AGCILQGYLL KPMTEAFEKN PNISNLMCAF 400
QTEIRAGLQN YRDMVALITS KLEVSIPVLS ASLNYVTAMF TPTLKYGQLV 450
SLQRDVFGRH GYERVDKDGR ESFQWPELQ 479
Length:479
Mass (Da):52,154
Last modified:July 1, 1993 - v1
Checksum:i64FED260915ABC2F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65623 Genomic DNA. Translation: CAA46577.1.
PIRiA48565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65623 Genomic DNA. Translation: CAA46577.1 .
PIRi A48565.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PGJ X-ray 2.82 A/B 2-479 [» ]
ProteinModelPortali P31072.
SMRi P31072. Positions 2-479.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00115 ; UER00410 .

Miscellaneous databases

EvolutionaryTracei P31072.

Family and domain databases

Gene3Di 1.10.1040.10. 1 hit.
1.20.5.320. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_decarbox.
IPR006115. 6PGDH_NADP-bd.
IPR006184. 6PGdom_BS.
IPR013328. DH_multihelical.
IPR012284. Fibritin/6PGD_C-extension.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000109. 6PGD. 1 hit.
SUPFAMi SSF48179. SSF48179. 1 hit.
TIGRFAMsi TIGR00873. gnd. 1 hit.
PROSITEi PS00461. 6PGD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A 6-phosphogluconate dehydrogenase gene from Trypanosoma brucei."
    Barrett M.P., le Page R.W.F.
    Mol. Biochem. Parasitol. 57:89-100(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 427.
  2. "A 2.8-A resolution structure of 6-phosphogluconate dehydrogenase from the protozoan parasite Trypanosoma brucei: comparison with the sheep enzyme accounts for differences in activity with coenzyme and substrate analogues."
    Phillips C., Dohnalek J., Gover S., Barrett M.P., Adams M.J.
    J. Mol. Biol. 282:667-681(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), SUBUNIT.

Entry informationi

Entry namei6PGD_TRYBB
AccessioniPrimary (citable) accession number: P31072
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 16, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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