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Protein

Voltage-gated potassium channel Kch

Gene

kch

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

K+-specific ion channel. May play a role in the defense against osmotic shock.2 Publications

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

GO - Biological processi

  • potassium ion transport Source: EcoCyc
  • protein homotetramerization Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:KCH-MONOMER.
ECOL316407:JW1242-MONOMER.
MetaCyc:KCH-MONOMER.

Protein family/group databases

TCDBi1.A.1.13.1. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-gated potassium channel Kch
Gene namesi
Name:kch
Ordered Locus Names:b1250, JW1242
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11606. kch.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121CytoplasmicSequence analysisAdd
BLAST
Transmembranei22 – 4120Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini42 – 6322PeriplasmicSequence analysisAdd
BLAST
Transmembranei64 – 8320Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini84 – 863CytoplasmicSequence analysis
Transmembranei87 – 10418Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini105 – 1106PeriplasmicSequence analysis
Transmembranei111 – 12717Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini128 – 14013CytoplasmicSequence analysisAdd
BLAST
Transmembranei141 – 16020Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini161 – 19939PeriplasmicSequence analysisAdd
BLAST
Transmembranei200 – 22021Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini221 – 417197Cytoplasmic2 PublicationsAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Voltage-gated potassium channel KchPRO_0000054096Add
BLAST

Proteomic databases

PaxDbiP31069.

Interactioni

Subunit structurei

Dimer.

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261503. 12 interactions.
DIPiDIP-10055N.
IntActiP31069. 1 interaction.
STRINGi511145.b1250.

Structurei

Secondary structure

1
417
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi246 – 2494Combined sources
Helixi253 – 26412Combined sources
Beta strandi269 – 2735Combined sources
Helixi277 – 28711Combined sources
Beta strandi292 – 2965Combined sources
Helixi301 – 3077Combined sources
Turni308 – 3114Combined sources
Beta strandi313 – 3175Combined sources
Helixi322 – 33514Combined sources
Beta strandi337 – 3393Combined sources
Beta strandi341 – 3444Combined sources
Helixi348 – 3503Combined sources
Helixi351 – 3555Combined sources
Beta strandi360 – 3634Combined sources
Helixi365 – 37713Combined sources
Helixi384 – 3896Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ID1X-ray2.40A/B241-393[»]
ProteinModelPortaliP31069.
SMRiP31069. Positions 143-393.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31069.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini245 – 371127RCK N-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi185 – 1906Selectivity filter

Sequence similaritiesi

Belongs to the potassium channel family.Curated
Contains 1 RCK N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107SVH. Bacteria.
COG1226. LUCA.
InParanoidiP31069.
KOiK10716.
OMAiFEGINTI.
OrthoDBiEOG63NMQN.
PhylomeDBiP31069.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR013099. K_chnl_dom.
IPR016040. NAD(P)-bd_dom.
IPR003148. RCK_N.
[Graphical view]
PfamiPF07885. Ion_trans_2. 1 hit.
PF02254. TrkA_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS51201. RCK_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHWATFKQT ATNLWVTLRH DILALAVFLN GLLIFKTIYG MSVNLLDIFH
60 70 80 90 100
IKAFSELDLS LLANAPLFML GVFLVLNSIG LLFRAKLAWA ISIILLLIAL
110 120 130 140 150
IYTLHFYPWL KFSIGFCIFT LVFLLILRKD FSHSSAAAGT IFAFISFTTL
160 170 180 190 200
LFYSTYGALY LSEGFNPRIE SLMTAFYFSI ETMSTVGYGD IVPVSESARL
210 220 230 240 250
FTISVIISGI TVFATSMTSI FGPLIRGGFN KLVKGNNHTM HRKDHFIVCG
260 270 280 290 300
HSILAINTIL QLNQRGQNVT VISNLPEDDI KQLEQRLGDN ADVIPGDSND
310 320 330 340 350
SSVLKKAGID RCRAILALSD NDADNAFVVL SAKDMSSDVK TVLAVSDSKN
360 370 380 390 400
LNKIKMVHPD IILSPQLFGS EILARVLNGE EINNDMLVSM LLNSGHGIFS
410
DNDELETKAD SKESAQK
Length:417
Mass (Da):46,063
Last modified:July 1, 1993 - v1
Checksum:iB2F803435001FB6E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti5 – 51A → T in strain: ECOR 52 and ECOR 60.
Natural varianti13 – 131N → K in strain: ECOR 52 and ECOR 60.
Natural varianti223 – 2231P → S in strain: ECOR 4.
Natural varianti405 – 4051L → Q in strain: ECOR 4, ECOR 16, ECOR 28, ECOR 31, ECOR 37, ECOR 46, ECOR 50, ECOR 52, ECOR 60 and ECOR 71.
Natural varianti406 – 4061E → K in strain: ECOR 46.
Natural varianti411 – 4111S → N in strain: ECOR 46.
Natural varianti414 – 4141S → L in strain: ECOR 46.
Natural varianti415 – 4151A → V in strain: ECOR 28.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12044 Genomic DNA. Translation: AAA66947.1.
U24195 Genomic DNA. Translation: AAB60071.1.
U24196 Genomic DNA. Translation: AAB60079.1.
U24197 Genomic DNA. Translation: AAB60087.1.
U24198 Genomic DNA. Translation: AAB60095.1.
U24199 Genomic DNA. Translation: AAB60103.1.
U24200 Genomic DNA. Translation: AAB60111.1.
U24201 Genomic DNA. Translation: AAB60119.1.
U24202 Genomic DNA. Translation: AAB60127.1.
U24203 Genomic DNA. Translation: AAB60135.1.
U24204 Genomic DNA. Translation: AAB60143.1.
U24205 Genomic DNA. Translation: AAB60151.1.
U24206 Genomic DNA. Translation: AAB60159.1.
U00096 Genomic DNA. Translation: AAC74332.1.
AP009048 Genomic DNA. Translation: BAA14782.1.
PIRiA55252.
T45507.
RefSeqiNP_415766.1. NC_000913.3.
WP_001295624.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74332; AAC74332; b1250.
BAA14782; BAA14782; BAA14782.
GeneIDi945841.
KEGGiecj:JW1242.
eco:b1250.
PATRICi32117760. VBIEscCol129921_1300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12044 Genomic DNA. Translation: AAA66947.1.
U24195 Genomic DNA. Translation: AAB60071.1.
U24196 Genomic DNA. Translation: AAB60079.1.
U24197 Genomic DNA. Translation: AAB60087.1.
U24198 Genomic DNA. Translation: AAB60095.1.
U24199 Genomic DNA. Translation: AAB60103.1.
U24200 Genomic DNA. Translation: AAB60111.1.
U24201 Genomic DNA. Translation: AAB60119.1.
U24202 Genomic DNA. Translation: AAB60127.1.
U24203 Genomic DNA. Translation: AAB60135.1.
U24204 Genomic DNA. Translation: AAB60143.1.
U24205 Genomic DNA. Translation: AAB60151.1.
U24206 Genomic DNA. Translation: AAB60159.1.
U00096 Genomic DNA. Translation: AAC74332.1.
AP009048 Genomic DNA. Translation: BAA14782.1.
PIRiA55252.
T45507.
RefSeqiNP_415766.1. NC_000913.3.
WP_001295624.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ID1X-ray2.40A/B241-393[»]
ProteinModelPortaliP31069.
SMRiP31069. Positions 143-393.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261503. 12 interactions.
DIPiDIP-10055N.
IntActiP31069. 1 interaction.
STRINGi511145.b1250.

Protein family/group databases

TCDBi1.A.1.13.1. the voltage-gated ion channel (vic) superfamily.

Proteomic databases

PaxDbiP31069.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74332; AAC74332; b1250.
BAA14782; BAA14782; BAA14782.
GeneIDi945841.
KEGGiecj:JW1242.
eco:b1250.
PATRICi32117760. VBIEscCol129921_1300.

Organism-specific databases

EchoBASEiEB1563.
EcoGeneiEG11606. kch.

Phylogenomic databases

eggNOGiENOG4107SVH. Bacteria.
COG1226. LUCA.
InParanoidiP31069.
KOiK10716.
OMAiFEGINTI.
OrthoDBiEOG63NMQN.
PhylomeDBiP31069.

Enzyme and pathway databases

BioCyciEcoCyc:KCH-MONOMER.
ECOL316407:JW1242-MONOMER.
MetaCyc:KCH-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP31069.
PROiP31069.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR013099. K_chnl_dom.
IPR016040. NAD(P)-bd_dom.
IPR003148. RCK_N.
[Graphical view]
PfamiPF07885. Ion_trans_2. 1 hit.
PF02254. TrkA_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS51201. RCK_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An Escherichia coli homologue of eukaryotic potassium channel proteins."
    Milkman R.
    Proc. Natl. Acad. Sci. U.S.A. 91:3510-3514(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Milkman R.
    Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 and Various ECOR strains.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Membrane topology of Kch, a putative K+ channel from Escherichia coli."
    Johansson M., von Heijne G.
    J. Biol. Chem. 271:25912-25915(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
    Strain: K12.
  7. "Gain-of-function mutations indicate that Escherichia coli Kch forms a functional K+ conduit in vivo."
    Kuo M.M., Saimi Y., Kung C.
    EMBO J. 22:4049-4058(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  9. "Structure of the RCK domain from the E. coli K+ channel and demonstration of its presence in the human BK channel."
    Jiang Y., Pico A., Cadene M., Chait B.T., MacKinnon R.
    Neuron 29:593-601(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 241-393, DIMERIZATION.

Entry informationi

Entry nameiKCH_ECOLI
AccessioniPrimary (citable) accession number: P31069
Secondary accession number(s): P94716
, P94717, P94723, P94729, P97198, P97225
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: January 20, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.