P31069 (KCH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Voltage-gated potassium channel Kch

Gene names

Name:	kch
Ordered Locus Names:	b1250, JW1242

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	K+-specific ion channel. May play a role in the defense against osmotic shock. Ref.1 Ref.7
Subunit structure	Dimer. Ref.9
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.6.
Sequence similarities	Belongs to the potassium channel family. Contains 1 RCK N-terminal domain.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ion channel
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	potassium ion transport Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 417

417

Voltage-gated potassium channel Kch

PRO_0000054096

Regions

Topological domain

1 – 21

21

Cytoplasmic Potential

Transmembrane

22 – 41

20

Helical; Name=1; Potential

Topological domain

42 – 63

22

Periplasmic Potential

Transmembrane

64 – 83

20

Helical; Name=2; Potential

Topological domain

84 – 86

3

Cytoplasmic Potential

Transmembrane

87 – 104

18

Helical; Name=3; Potential

Topological domain

105 – 110

6

Periplasmic Potential

Transmembrane

111 – 127

17

Helical; Name=4; Potential

Topological domain

128 – 140

13

Cytoplasmic Potential

Transmembrane

141 – 160

20

Helical; Name=5; Potential

Topological domain

161 – 199

39

Periplasmic Potential

Transmembrane

200 – 220

21

Helical; Name=6; Potential

Topological domain

221 – 417

197

Cytoplasmic Ref.6 Ref.8

Domain

245 – 371

127

RCK N-terminal

Motif

185 – 190

6

Selectivity filter

Natural variations

Natural variant

5

1

A → T in strain: ECOR 52 and ECOR 60.

Natural variant

13

1

N → K in strain: ECOR 52 and ECOR 60.

Natural variant

223

1

P → S in strain: ECOR 4.

Natural variant

405

1

L → Q in strain: ECOR 4, ECOR 16, ECOR 28, ECOR 31, ECOR 37, ECOR 46, ECOR 50, ECOR 52, ECOR 60 and ECOR 71.

Natural variant

406

1

E → K in strain: ECOR 46.

Natural variant

411

1

S → N in strain: ECOR 46.

Natural variant

414

1

S → L in strain: ECOR 46.

Natural variant

415

1

A → V in strain: ECOR 28.

Secondary structure

1

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417

Helix Strand Turn

Details...

Beta strand

246 – 249

4

Helix

253 – 264

12

Beta strand

269 – 273

5

Helix

277 – 287

11

Beta strand

292 – 296

5

Helix

301 – 307

7

Turn

308 – 311

4

Beta strand

313 – 317

5

Helix

322 – 335

14

Beta strand

337 – 339

3

Beta strand

341 – 344

4

Helix

348 – 350

3

Helix

351 – 355

5

Beta strand

360 – 363

4

Helix

365 – 377

13

Helix

384 – 389

6

Sequences

	Sequence		Length	Mass (Da)
	P31069 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: B2F803435001FB6E	FASTA	417	46,063
	10 20 30 40 50 60 MSHWATFKQT ATNLWVTLRH DILALAVFLN GLLIFKTIYG MSVNLLDIFH IKAFSELDLS 70 80 90 100 110 120 LLANAPLFML GVFLVLNSIG LLFRAKLAWA ISIILLLIAL IYTLHFYPWL KFSIGFCIFT 130 140 150 160 170 180 LVFLLILRKD FSHSSAAAGT IFAFISFTTL LFYSTYGALY LSEGFNPRIE SLMTAFYFSI 190 200 210 220 230 240 ETMSTVGYGD IVPVSESARL FTISVIISGI TVFATSMTSI FGPLIRGGFN KLVKGNNHTM 250 260 270 280 290 300 HRKDHFIVCG HSILAINTIL QLNQRGQNVT VISNLPEDDI KQLEQRLGDN ADVIPGDSND 310 320 330 340 350 360 SSVLKKAGID RCRAILALSD NDADNAFVVL SAKDMSSDVK TVLAVSDSKN LNKIKMVHPD 370 380 390 400 410 IILSPQLFGS EILARVLNGE EINNDMLVSM LLNSGHGIFS DNDELETKAD SKESAQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An Escherichia coli homologue of eukaryotic potassium channel proteins." Milkman R. Proc. Natl. Acad. Sci. U.S.A. 91:3510-3514(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	Milkman R. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 and Various ECOR strains.
[3]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Membrane topology of Kch, a putative K+ channel from Escherichia coli." Johansson M., von Heijne G. J. Biol. Chem. 271:25912-25915(1996) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TOPOLOGY. Strain: K12.
[7]	"Gain-of-function mutations indicate that Escherichia coli Kch forms a functional K+ conduit in vivo." Kuo M.M., Saimi Y., Kung C. EMBO J. 22:4049-4058(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
[9]	"Structure of the RCK domain from the E. coli K+ channel and demonstration of its presence in the human BK channel." Jiang Y., Pico A., Cadene M., Chait B.T., MacKinnon R. Neuron 29:593-601(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 241-393, DIMERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L12044 Genomic DNA. Translation: AAA66947.1.
U24195 Genomic DNA. Translation: AAB60071.1.
U24196 Genomic DNA. Translation: AAB60079.1.
U24197 Genomic DNA. Translation: AAB60087.1.
U24198 Genomic DNA. Translation: AAB60095.1.
U24199 Genomic DNA. Translation: AAB60103.1.
U24200 Genomic DNA. Translation: AAB60111.1.
U24201 Genomic DNA. Translation: AAB60119.1.
U24202 Genomic DNA. Translation: AAB60127.1.
U24203 Genomic DNA. Translation: AAB60135.1.
U24204 Genomic DNA. Translation: AAB60143.1.
U24205 Genomic DNA. Translation: AAB60151.1.
U24206 Genomic DNA. Translation: AAB60159.1.
U00096 Genomic DNA. Translation: AAC74332.1.
AP009048 Genomic DNA. Translation: BAA14782.1.

PIR

A55252.
T45507.

RefSeq

NP_415766.1. NC_000913.2.
YP_489518.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ID1	X-ray	2.40	A/B	241-393	[»]

ProteinModelPortal

P31069.

SMR

P31069. Positions 143-393.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10055N.

IntAct

P31069. 1 interaction.

STRING

511145.b1250.

Protein family/group databases

TCDB

1.A.1.13.1. voltage-gated ion channel (VIC) superfamily.

Proteomic databases

PaxDb

P31069.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74332; AAC74332; b1250.
BAA14782; BAA14782; BAA14782.

GeneID

12932509.
945841.

KEGG

ecj:Y75_p1224.
eco:b1250.

PATRIC

32117760. VBIEscCol129921_1300.

Organism-specific databases

EchoBASE

EB1563.

EcoGene

EG11606. kch.

Phylogenomic databases

eggNOG

COG1226.

KO

K10716.

OMA

DAFYFTL.

ProtClustDB

PRK10537.

Enzyme and pathway databases

BioCyc

EcoCyc:KCH-MONOMER.
ECOL316407:JW1242-MONOMER.

Gene expression databases

Genevestigator

P31069.

Family and domain databases

Gene3D

3.40.50.720. 1 hit.

InterPro

IPR013099. Ion_trans_2.
IPR016040. NAD(P)-bd_dom.
IPR003148. RCK_N.
[Graphical view]

Pfam

PF07885. Ion_trans_2. 1 hit.
PF02254. TrkA_N. 1 hit.
[Graphical view]

PROSITE

PS51201. RCK_N. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P31069.

Entry information

Entry name

KCH_ECOLI

Accession

Primary (citable) accession number: P31069
Secondary accession number(s): P94716 , P94717, P94723, P94729, P97198, P97225

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program