Reviewed, UniProtKB/Swiss-Prot P31042 (CD28_RAT)

Names and origin

Protein names

Recommended name:
    T-cell-specific surface glycoprotein CD28
Alternative name(s):
    CD_antigen=CD28

Gene names

Name:

Cd28

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Possibly involved in T-cell activation By similarity.
Subunit structure	Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Immunoglobulin domain Signal Transmembrane
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	T cell costimulation Inferred from mutant phenotype. Source: RGD immune response Traceable author statement. Source: RGD positive regulation of interleukin-2 biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of mitosis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane raft Inferred from direct assay. Source: RGD
Molecular function	coreceptor activity Non-traceable author statement. Source: RGD protein homodimerization activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Chain	20 – 218	199	T-cell-specific surface glycoprotein CD28		PRO_0000014654
Regions
	Topological domain	20 – 150	131	Extracellular Potential
	Transmembrane	151 – 177	27	Potential
	Topological domain	178 – 218	41	Cytoplasmic Potential
	Domain	29 – 138	110	Ig-like V-type
Amino acid modifications
	Modified residue	189	1	Phosphotyrosine By similarity
	Modified residue	207	1	Phosphotyrosine By similarity
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	106	1	N-linked (GlcNAc...) Potential
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Disulfide bond	41 ↔ 113		By similarity
	Disulfide bond	67 ↔ 87		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31042-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 2E151C8F324C0B6E	FASTA	218	25,170
	10 20 30 40 50 60 MTLRLLFLAL SFFSVQVTEN KILVKQSPLL VVDNNEVSLS CRYSYNLLAK EFRASLYKGV 70 80 90 100 110 120 NSDVEVCVGN GNFTYQPQFR PNVGFNCDGN FDNETVTFRL WNLDVNHTDI YFCKIEVMYP 130 140 150 160 170 180 PPYLDNEKSN GTIIHIKEKH LCHAQTSPKL FWPLVVVAGV LLCYGLLVTV TLCIIWTNSR 190 200 210 RNRLLQSDYM NMTPRRLGPT RKHYQPYAPA RDFAAYRP

References

[1]	"Identification of a cDNA encoding the rat CD28 homologue." Clark G.J., Dallman M.J. Immunogenetics 35:54-57(1992) [PubMed: 1309509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: DA. Tissue: Lymphoid.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55288 mRNA. Translation: CAA39003.1.
IPI	IPI00208889.
PIR	S24413.
RefSeq	NP_037253.1.
UniGene	Rn.10327
3D structure databases
HSSP	HSSP built from PDB template 1I85 based on UniProtKB P16410.
SMR	P31042. Positions 20-137.
ModBase	Search...
Protein-protein interaction databases
STRING	P31042.
Genome annotation databases
Ensembl	ENSRNOT00000013701; ENSRNOP00000013701; ENSRNOG00000010283; Rattus norvegicus. [Genome view]
GeneID	25660.
KEGG	rno:25660.
UCSC	NM_013121. rat.
Organism-specific databases
CTD	25660.
RGD	2299. Cd28.
Phylogenomic databases
HOVERGEN	P31042.
Gene expression databases
ArrayExpress	P31042.
Genevestigator	P31042.
GermOnline	ENSRNOG00000010283. Rattus norvegicus.
Family and domain databases
InterPro	IPR008093. CD28. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF07686. V-set. 1 hit. [Graphical view]
PRINTS	PR01717. CD28ANTIGEN.
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	607561.

Entry information

Entry name

CD28_RAT

Accession

Primary (citable) accession number: P31042

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	October 13, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)