ID   CD28_MOUSE              Reviewed;         218 AA.
AC   P31041; Q6GSH7;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 2.
DT   27-MAR-2024, entry version 178.
DE   RecName: Full=T-cell-specific surface glycoprotein CD28;
DE   AltName: CD_antigen=CD28;
DE   Flags: Precursor;
GN   Name=Cd28;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2157764;
RA   Gross J.A., St John T., Allison J.P.;
RT   "The murine homologue of the T lymphocyte antigen CD28. Molecular cloning
RT   and cell surface expression.";
RL   J. Immunol. 144:3201-3210(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Thymus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 156-218.
RX   PubMed=1713603;
RA   Harper K., Balzano C., Rouvier E., Mattei M.-G., Luciani M.-F.,
RA   Golstein P.;
RT   "CTLA-4 and CD28 activated lymphocyte molecules are closely related in both
RT   mouse and human as to sequence, message expression, gene structure, and
RT   chromosomal location.";
RL   J. Immunol. 147:1037-1044(1991).
CC   -!- FUNCTION: Involved in T-cell activation, the induction of cell
CC       proliferation and cytokine production and promotion of T-cell survival.
CC       Enhances the production of IL4 and IL10 in T-cells in conjunction with
CC       TCR/CD3 ligation and CD40L costimulation.
CC       {ECO:0000250|UniProtKB:P10747}.
CC   -!- SUBUNIT: Homodimer; disulfide-linked. Interacts with DUSP14. Binds to
CC       CD80/B7-1 and CD86/B7-2/B70. Interacts with GRB2 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
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DR   EMBL; M34563; AAA37395.1; -; mRNA.
DR   EMBL; AL646054; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AL672024; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CR936842; CAM13249.1; -; Genomic_DNA.
DR   EMBL; CH466548; EDL00156.1; -; Genomic_DNA.
DR   EMBL; BC064058; AAH64058.1; -; mRNA.
DR   EMBL; M74361; AAA37396.1; -; mRNA.
DR   CCDS; CCDS14992.1; -.
DR   PIR; A43523; A43523.
DR   PIR; I49584; I49584.
DR   RefSeq; NP_031668.3; NM_007642.4.
DR   PDB; 2NAE; NMR; -; A=177-218.
DR   PDBsum; 2NAE; -.
DR   AlphaFoldDB; P31041; -.
DR   SMR; P31041; -.
DR   DIP; DIP-60858N; -.
DR   IntAct; P31041; 3.
DR   STRING; 10090.ENSMUSP00000027165; -.
DR   GlyCosmos; P31041; 4 sites, No reported glycans.
DR   GlyGen; P31041; 4 sites.
DR   iPTMnet; P31041; -.
DR   PhosphoSitePlus; P31041; -.
DR   SwissPalm; P31041; -.
DR   EPD; P31041; -.
DR   PaxDb; 10090-ENSMUSP00000027165; -.
DR   PeptideAtlas; P31041; -.
DR   ProteomicsDB; 280017; -.
DR   Antibodypedia; 19958; 3331 antibodies from 49 providers.
DR   DNASU; 12487; -.
DR   Ensembl; ENSMUST00000027165.3; ENSMUSP00000027165.3; ENSMUSG00000026012.3.
DR   GeneID; 12487; -.
DR   KEGG; mmu:12487; -.
DR   UCSC; uc007bev.2; mouse.
DR   AGR; MGI:88327; -.
DR   CTD; 940; -.
DR   MGI; MGI:88327; Cd28.
DR   VEuPathDB; HostDB:ENSMUSG00000026012; -.
DR   eggNOG; ENOG502SAVP; Eukaryota.
DR   GeneTree; ENSGT00530000063873; -.
DR   HOGENOM; CLU_085095_1_0_1; -.
DR   InParanoid; P31041; -.
DR   OMA; YSHQLQF; -.
DR   OrthoDB; 4108912at2759; -.
DR   PhylomeDB; P31041; -.
DR   TreeFam; TF335679; -.
DR   Reactome; R-MMU-1257604; PIP3 activates AKT signaling.
DR   Reactome; R-MMU-389356; CD28 co-stimulation.
DR   Reactome; R-MMU-389357; CD28 dependent PI3K/Akt signaling.
DR   Reactome; R-MMU-389359; CD28 dependent Vav1 pathway.
DR   Reactome; R-MMU-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
DR   BioGRID-ORCS; 12487; 2 hits in 117 CRISPR screens.
DR   PRO; PR:P31041; -.
DR   Proteomes; UP000000589; Chromosome 1.
DR   RNAct; P31041; Protein.
DR   Bgee; ENSMUSG00000026012; Expressed in thymus and 56 other cell types or tissues.
DR   GO; GO:0009986; C:cell surface; ISO:MGI.
DR   GO; GO:0009897; C:external side of plasma membrane; IDA:MGI.
DR   GO; GO:0001772; C:immunological synapse; IDA:MGI.
DR   GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR   GO; GO:0098636; C:protein complex involved in cell adhesion; ISO:MGI.
DR   GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR   GO; GO:0002020; F:protease binding; ISO:MGI.
DR   GO; GO:0019901; F:protein kinase binding; IPI:MGI.
DR   GO; GO:0097190; P:apoptotic signaling pathway; IDA:MGI.
DR   GO; GO:0035739; P:CD4-positive, alpha-beta T cell proliferation; IDA:MGI.
DR   GO; GO:0006955; P:immune response; IEA:InterPro.
DR   GO; GO:0010629; P:negative regulation of gene expression; ISO:MGI.
DR   GO; GO:0045060; P:negative thymic T cell selection; IMP:MGI.
DR   GO; GO:0043491; P:phosphatidylinositol 3-kinase/protein kinase B signal transduction; IDA:MGI.
DR   GO; GO:2000563; P:positive regulation of CD4-positive, alpha-beta T cell proliferation; IDA:MGI.
DR   GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR   GO; GO:0002863; P:positive regulation of inflammatory response to antigenic stimulus; IMP:MGI.
DR   GO; GO:0032733; P:positive regulation of interleukin-10 production; ISS:UniProtKB.
DR   GO; GO:0032743; P:positive regulation of interleukin-2 production; IMP:MGI.
DR   GO; GO:0032753; P:positive regulation of interleukin-4 production; ISS:UniProtKB.
DR   GO; GO:0048304; P:positive regulation of isotype switching to IgG isotypes; IMP:MGI.
DR   GO; GO:0045840; P:positive regulation of mitotic nuclear division; ISS:UniProtKB.
DR   GO; GO:0051897; P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; IDA:MGI.
DR   GO; GO:0042102; P:positive regulation of T cell proliferation; IDA:MGI.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IGI:MGI.
DR   GO; GO:0045589; P:regulation of regulatory T cell differentiation; IGI:MGI.
DR   GO; GO:0045066; P:regulatory T cell differentiation; ISO:MGI.
DR   GO; GO:0042110; P:T cell activation; ISO:MGI.
DR   GO; GO:0031295; P:T cell costimulation; IDA:MGI.
DR   GO; GO:0042098; P:T cell proliferation; IDA:MGI.
DR   GO; GO:0050852; P:T cell receptor signaling pathway; IMP:MGI.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; IGI:MGI.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR008093; CD28.
DR   InterPro; IPR040216; CTLA4/CD28.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR013106; Ig_V-set.
DR   PANTHER; PTHR11494; CYTOTOXIC T-LYMPHOCYTE PROTEIN; 1.
DR   PANTHER; PTHR11494:SF7; T-CELL-SPECIFIC SURFACE GLYCOPROTEIN CD28; 1.
DR   Pfam; PF07686; V-set; 1.
DR   PRINTS; PR01717; CD28ANTIGEN.
DR   SUPFAM; SSF48726; Immunoglobulin; 1.
DR   Genevisible; P31041; MM.
PE   1: Evidence at protein level;
KW   3D-structure; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW   Membrane; Phosphoprotein; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000250"
FT   CHAIN           20..218
FT                   /note="T-cell-specific surface glycoprotein CD28"
FT                   /id="PRO_0000014653"
FT   TOPO_DOM        20..150
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        151..177
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        178..218
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          29..138
FT                   /note="Ig-like V-type"
FT   MOD_RES         187
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P10747"
FT   MOD_RES         189
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:P10747"
FT   MOD_RES         207
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:P10747"
FT   CARBOHYD        72
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        93
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        106
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        130
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        41..113
FT                   /evidence="ECO:0000250"
FT   DISULFID        67..87
FT                   /evidence="ECO:0000250"
FT   CONFLICT        187..189
FT                   /note="SDY -> VTT (in Ref. 1; AAA37395)"
FT                   /evidence="ECO:0000305"
FT   STRAND          185..187
FT                   /evidence="ECO:0007829|PDB:2NAE"
FT   TURN            214..216
FT                   /evidence="ECO:0007829|PDB:2NAE"
SQ   SEQUENCE   218 AA;  25243 MW;  C8E1339DD7E53CA6 CRC64;
     MTLRLLFLAL NFFSVQVTEN KILVKQSPLL VVDSNEVSLS CRYSYNLLAK EFRASLYKGV
     NSDVEVCVGN GNFTYQPQFR SNAEFNCDGD FDNETVTFRL WNLHVNHTDI YFCKIEFMYP
     PPYLDNERSN GTIIHIKEKH LCHTQSSPKL FWALVVVAGV LFCYGLLVTV ALCVIWTNSR
     RNRLLQSDYM NMTPRRPGLT RKPYQPYAPA RDFAAYRP
//