P31041 (CD28_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
T-cell-specific surface glycoprotein CD28

Alternative name(s):
CD_antigen=CD28

Gene names

Name:

Cd28

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Possibly involved in T-cell activation.
Subunit structure	Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell costimulation Inferred from direct assay PubMed 10429671PubMed 12925852PubMed 15240667PubMed 8125140PubMed 9368605. Source: MGI apoptotic signaling pathway Inferred from direct assay PubMed 10429671. Source: MGI immune response Inferred from electronic annotation. Source: InterPro negative thymic T cell selection Inferred from mutant phenotype PubMed 10429671. Source: MGI positive regulation of alpha-beta T cell proliferation Inferred from direct assay PubMed 14973438PubMed 8125140PubMed 9368605. Source: MGI positive regulation of inflammatory response to antigenic stimulus Inferred from mutant phenotype PubMed 10791997. Source: MGI positive regulation of interleukin-2 biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of isotype switching to IgG isotypes Inferred from mutant phenotype PubMed 7688139. Source: MGI positive regulation of mitosis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction PubMed 16809611. Source: MGI regulatory T cell differentiation Inferred from electronic annotation. Source: Compara
Cellular_component	external side of plasma membrane Inferred from direct assay PubMed 12874832PubMed 7790813PubMed 8125140. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Chain	20 – 218	199	T-cell-specific surface glycoprotein CD28		PRO_0000014653
Regions
	Topological domain	20 – 150	131	Extracellular Potential
	Transmembrane	151 – 177	27	Helical; Potential
	Topological domain	178 – 218	41	Cytoplasmic Potential
	Domain	29 – 138	110	Ig-like V-type
Amino acid modifications
	Modified residue	187	1	Phosphoserine By similarity
	Modified residue	189	1	Phosphotyrosine By similarity
	Modified residue	207	1	Phosphotyrosine By similarity
	Modified residue	216	1	Phosphotyrosine By similarity
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	106	1	N-linked (GlcNAc...) Potential
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Disulfide bond	41 ↔ 113		By similarity
	Disulfide bond	67 ↔ 87		By similarity
Experimental info
	Sequence conflict	187 – 189	3	SDY → VTT in AAA37395. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P31041 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: C8E1339DD7E53CA6	FASTA	218	25,243
	10 20 30 40 50 60 MTLRLLFLAL NFFSVQVTEN KILVKQSPLL VVDSNEVSLS CRYSYNLLAK EFRASLYKGV 70 80 90 100 110 120 NSDVEVCVGN GNFTYQPQFR SNAEFNCDGD FDNETVTFRL WNLHVNHTDI YFCKIEFMYP 130 140 150 160 170 180 PPYLDNERSN GTIIHIKEKH LCHTQSSPKL FWALVVVAGV LFCYGLLVTV ALCVIWTNSR 190 200 210 RNRLLQSDYM NMTPRRPGLT RKPYQPYAPA RDFAAYRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The murine homologue of the T lymphocyte antigen CD28. Molecular cloning and cell surface expression." Gross J.A., St John T., Allison J.P. J. Immunol. 144:3201-3210(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Thymus.
[5]	"CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequence, message expression, gene structure, and chromosomal location." Harper K., Balzano C., Rouvier E., Mattei M.-G., Luciani M.-F., Golstein P. J. Immunol. 147:1037-1044(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-218.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34563 mRNA. Translation: AAA37395.1. AL646054 Genomic DNA. Translation: CAI24197.1. AL672024 Genomic DNA. Translation: CAI26227.1. CR936842 Genomic DNA. Translation: CAM13249.1. CH466548 Genomic DNA. Translation: EDL00156.1. BC064058 mRNA. Translation: AAH64058.1. M74361 mRNA. Translation: AAA37396.1.
IPI	IPI00318069. IPI00387437.
PIR	A43523. I49584.
RefSeq	NP_031668.3. NM_007642.4.
UniGene	Mm.255003.
3D structure databases
ProteinModelPortal	P31041.
SMR	P31041. Positions 20-137.
ModBase	Search...
Protein-protein interaction databases
IntAct	P31041. 1 interaction.
STRING	10090.ENSMUSP00000027165.
PTM databases
PhosphoSite	P31041.
Proteomic databases
PRIDE	P31041.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027165; ENSMUSP00000027165; ENSMUSG00000026012.
GeneID	12487.
KEGG	mmu:12487.
Organism-specific databases
CTD	940.
MGI	MGI:88327. Cd28.
Phylogenomic databases
eggNOG	NOG48152.
GeneTree	ENSGT00530000063873.
HOGENOM	HOG000276892.
HOVERGEN	HBG004094.
InParanoid	P31041.
KO	K06470.
OMA	TENKILV.
OrthoDB	EOG498V1S.
Gene expression databases
CleanEx	MM_CD28.
Genevestigator	P31041.
GermOnline	ENSMUSG00000026012. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 1 hit.
InterPro	IPR008093. CD28. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set. [Graphical view]
Pfam	PF07686. V-set. 1 hit. [Graphical view]
PRINTS	PR01717. CD28ANTIGEN.
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	281396.
SOURCE	Search...

Entry information

Entry name

CD28_MOUSE

Accession

Primary (citable) accession number: P31041
Secondary accession number(s): Q6GSH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program