Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P31041 (CD28_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-cell-specific surface glycoprotein CD28
Alternative name(s):
CD_antigen=CD28
Gene names
Name:Cd28
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length218 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Possibly involved in T-cell activation.

Subunit structure

Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70 By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Cellular componentMembrane
   DomainImmunoglobulin domain
Signal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT cell costimulation

Inferred from direct assay PubMed 10429671PubMed 12925852PubMed 15240667PubMed 8125140PubMed 9368605. Source: MGI

apoptotic signaling pathway

Inferred from direct assay PubMed 10429671. Source: MGI

immune response

Inferred from electronic annotation. Source: InterPro

negative thymic T cell selection

Inferred from mutant phenotype PubMed 10429671. Source: MGI

positive regulation of T cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of alpha-beta T cell proliferation

Inferred from direct assay PubMed 14973438PubMed 8125140PubMed 9368605. Source: MGI

positive regulation of inflammatory response to antigenic stimulus

Inferred from mutant phenotype PubMed 10791997. Source: MGI

positive regulation of interleukin-2 biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of isotype switching to IgG isotypes

Inferred from mutant phenotype PubMed 7688139. Source: MGI

positive regulation of mitosis

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of signal transduction

Inferred from electronic annotation. Source: GOC

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 16809611. Source: MGI

regulatory T cell differentiation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentexternal side of plasma membrane

Inferred from direct assay PubMed 12874832PubMed 23793062PubMed 7790813PubMed 8125140. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 By similarity
Chain20 – 218199T-cell-specific surface glycoprotein CD28
PRO_0000014653

Regions

Topological domain20 – 150131Extracellular Potential
Transmembrane151 – 17727Helical; Potential
Topological domain178 – 21841Cytoplasmic Potential
Domain29 – 138110Ig-like V-type

Amino acid modifications

Modified residue1871Phosphoserine By similarity
Modified residue1891Phosphotyrosine By similarity
Modified residue2071Phosphotyrosine By similarity
Glycosylation721N-linked (GlcNAc...) Potential
Glycosylation931N-linked (GlcNAc...) Potential
Glycosylation1061N-linked (GlcNAc...) Potential
Glycosylation1301N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 113 By similarity
Disulfide bond67 ↔ 87 By similarity

Experimental info

Sequence conflict187 – 1893SDY → VTT in AAA37395. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P31041 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: C8E1339DD7E53CA6

FASTA21825,243
        10         20         30         40         50         60 
MTLRLLFLAL NFFSVQVTEN KILVKQSPLL VVDSNEVSLS CRYSYNLLAK EFRASLYKGV 

        70         80         90        100        110        120 
NSDVEVCVGN GNFTYQPQFR SNAEFNCDGD FDNETVTFRL WNLHVNHTDI YFCKIEFMYP 

       130        140        150        160        170        180 
PPYLDNERSN GTIIHIKEKH LCHTQSSPKL FWALVVVAGV LFCYGLLVTV ALCVIWTNSR 

       190        200        210 
RNRLLQSDYM NMTPRRPGLT RKPYQPYAPA RDFAAYRP 

« Hide

References

« Hide 'large scale' references
[1]"The murine homologue of the T lymphocyte antigen CD28. Molecular cloning and cell surface expression."
Gross J.A., St John T., Allison J.P.
J. Immunol. 144:3201-3210(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Thymus.
[5]"CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequence, message expression, gene structure, and chromosomal location."
Harper K., Balzano C., Rouvier E., Mattei M.-G., Luciani M.-F., Golstein P.
J. Immunol. 147:1037-1044(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-218.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34563 mRNA. Translation: AAA37395.1.
AL646054 Genomic DNA. Translation: CAI24197.1.
AL672024 Genomic DNA. Translation: CAI26227.1.
CR936842 Genomic DNA. Translation: CAM13249.1.
CH466548 Genomic DNA. Translation: EDL00156.1.
BC064058 mRNA. Translation: AAH64058.1.
M74361 mRNA. Translation: AAA37396.1.
PIRA43523.
I49584.
RefSeqNP_031668.3. NM_007642.4.
UniGeneMm.255003.

3D structure databases

ProteinModelPortalP31041.
SMRP31041. Positions 20-137.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP31041. 1 interaction.
STRING10090.ENSMUSP00000027165.

PTM databases

PhosphoSiteP31041.

Proteomic databases

PRIDEP31041.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027165; ENSMUSP00000027165; ENSMUSG00000026012.
GeneID12487.
KEGGmmu:12487.
UCSCuc007bev.2. mouse.
uc007bew.2. mouse.

Organism-specific databases

CTD940.
MGIMGI:88327. Cd28.

Phylogenomic databases

eggNOGNOG48152.
GeneTreeENSGT00530000063873.
HOGENOMHOG000276892.
HOVERGENHBG004094.
InParanoidP31041.
KOK06470.
OMASSKPFWA.
OrthoDBEOG7W6WMJ.
TreeFamTF335679.

Gene expression databases

CleanExMM_CD28.
GenevestigatorP31041.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR008093. CD28.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF07686. V-set. 1 hit.
[Graphical view]
PRINTSPR01717. CD28ANTIGEN.
SMARTSM00409. IG. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281396.
PROP31041.
SOURCESearch...

Entry information

Entry nameCD28_MOUSE
AccessionPrimary (citable) accession number: P31041
Secondary accession number(s): Q6GSH7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot