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P31041

- CD28_MOUSE

UniProt

P31041 - CD28_MOUSE

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Protein

T-cell-specific surface glycoprotein CD28

Gene

Cd28

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.By similarity

GO - Biological processi

  1. apoptotic signaling pathway Source: MGI
  2. immune response Source: InterPro
  3. negative thymic T cell selection Source: MGI
  4. positive regulation of alpha-beta T cell proliferation Source: MGI
  5. positive regulation of inflammatory response to antigenic stimulus Source: MGI
  6. positive regulation of interleukin-2 biosynthetic process Source: UniProtKB
  7. positive regulation of isotype switching to IgG isotypes Source: MGI
  8. positive regulation of mitosis Source: UniProtKB
  9. positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
  10. positive regulation of protein kinase B signaling Source: MGI
  11. positive regulation of T cell proliferation Source: UniProtKB
  12. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  13. regulatory T cell differentiation Source: Ensembl
  14. T cell costimulation Source: MGI
  15. T cell receptor signaling pathway Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_204081. CD28 co-stimulation.
REACT_226151. CD28 dependent PI3K/Akt signaling.
REACT_226341. PIP3 activates AKT signaling.
REACT_261451. CD28 dependent Vav1 pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell-specific surface glycoprotein CD28
Alternative name(s):
CD_antigen: CD28
Gene namesi
Name:Cd28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:88327. Cd28.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 150131ExtracellularSequence AnalysisAdd
BLAST
Transmembranei151 – 17727HelicalSequence AnalysisAdd
BLAST
Topological domaini178 – 21841CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. external side of plasma membrane Source: MGI
  2. immunological synapse Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919By similarityAdd
BLAST
Chaini20 – 218199T-cell-specific surface glycoprotein CD28PRO_0000014653Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 113By similarity
Disulfide bondi67 ↔ 87By similarity
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence Analysis
Modified residuei187 – 1871PhosphoserineBy similarity
Modified residuei189 – 1891PhosphotyrosineBy similarity
Modified residuei207 – 2071PhosphotyrosineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP31041.

PTM databases

PhosphoSiteiP31041.

Expressioni

Gene expression databases

CleanExiMM_CD28.
GenevestigatoriP31041.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. Interacts with GRB2 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-60858N.
IntActiP31041. 1 interaction.
STRINGi10090.ENSMUSP00000027165.

Structurei

3D structure databases

ProteinModelPortaliP31041.
SMRiP31041. Positions 20-137.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 138110Ig-like V-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG48152.
GeneTreeiENSGT00530000063873.
HOGENOMiHOG000276892.
HOVERGENiHBG004094.
InParanoidiP31041.
KOiK06470.
OMAiVNGNYSH.
OrthoDBiEOG7W6WMJ.
TreeFamiTF335679.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR008093. CD28.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
PRINTSiPR01717. CD28ANTIGEN.
SMARTiSM00409. IG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31041-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLRLLFLAL NFFSVQVTEN KILVKQSPLL VVDSNEVSLS CRYSYNLLAK
60 70 80 90 100
EFRASLYKGV NSDVEVCVGN GNFTYQPQFR SNAEFNCDGD FDNETVTFRL
110 120 130 140 150
WNLHVNHTDI YFCKIEFMYP PPYLDNERSN GTIIHIKEKH LCHTQSSPKL
160 170 180 190 200
FWALVVVAGV LFCYGLLVTV ALCVIWTNSR RNRLLQSDYM NMTPRRPGLT
210
RKPYQPYAPA RDFAAYRP
Length:218
Mass (Da):25,243
Last modified:October 3, 2012 - v2
Checksum:iC8E1339DD7E53CA6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti187 – 1893SDY → VTT in AAA37395. (PubMed:2157764)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34563 mRNA. Translation: AAA37395.1.
AL646054 Genomic DNA. Translation: CAI24197.1.
AL672024 Genomic DNA. Translation: CAI26227.1.
CR936842 Genomic DNA. Translation: CAM13249.1.
CH466548 Genomic DNA. Translation: EDL00156.1.
BC064058 mRNA. Translation: AAH64058.1.
M74361 mRNA. Translation: AAA37396.1.
CCDSiCCDS14992.1.
PIRiA43523.
I49584.
RefSeqiNP_031668.3. NM_007642.4.
UniGeneiMm.255003.

Genome annotation databases

EnsembliENSMUST00000027165; ENSMUSP00000027165; ENSMUSG00000026012.
GeneIDi12487.
KEGGimmu:12487.
UCSCiuc007bev.2. mouse.
uc007bew.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34563 mRNA. Translation: AAA37395.1 .
AL646054 Genomic DNA. Translation: CAI24197.1 .
AL672024 Genomic DNA. Translation: CAI26227.1 .
CR936842 Genomic DNA. Translation: CAM13249.1 .
CH466548 Genomic DNA. Translation: EDL00156.1 .
BC064058 mRNA. Translation: AAH64058.1 .
M74361 mRNA. Translation: AAA37396.1 .
CCDSi CCDS14992.1.
PIRi A43523.
I49584.
RefSeqi NP_031668.3. NM_007642.4.
UniGenei Mm.255003.

3D structure databases

ProteinModelPortali P31041.
SMRi P31041. Positions 20-137.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60858N.
IntActi P31041. 1 interaction.
STRINGi 10090.ENSMUSP00000027165.

PTM databases

PhosphoSitei P31041.

Proteomic databases

PRIDEi P31041.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027165 ; ENSMUSP00000027165 ; ENSMUSG00000026012 .
GeneIDi 12487.
KEGGi mmu:12487.
UCSCi uc007bev.2. mouse.
uc007bew.2. mouse.

Organism-specific databases

CTDi 940.
MGIi MGI:88327. Cd28.

Phylogenomic databases

eggNOGi NOG48152.
GeneTreei ENSGT00530000063873.
HOGENOMi HOG000276892.
HOVERGENi HBG004094.
InParanoidi P31041.
KOi K06470.
OMAi VNGNYSH.
OrthoDBi EOG7W6WMJ.
TreeFami TF335679.

Enzyme and pathway databases

Reactomei REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_204081. CD28 co-stimulation.
REACT_226151. CD28 dependent PI3K/Akt signaling.
REACT_226341. PIP3 activates AKT signaling.
REACT_261451. CD28 dependent Vav1 pathway.

Miscellaneous databases

NextBioi 281396.
PROi P31041.
SOURCEi Search...

Gene expression databases

CleanExi MM_CD28.
Genevestigatori P31041.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
InterProi IPR008093. CD28.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view ]
Pfami PF07686. V-set. 1 hit.
[Graphical view ]
PRINTSi PR01717. CD28ANTIGEN.
SMARTi SM00409. IG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The murine homologue of the T lymphocyte antigen CD28. Molecular cloning and cell surface expression."
    Gross J.A., St John T., Allison J.P.
    J. Immunol. 144:3201-3210(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Thymus.
  5. "CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequence, message expression, gene structure, and chromosomal location."
    Harper K., Balzano C., Rouvier E., Mattei M.-G., Luciani M.-F., Golstein P.
    J. Immunol. 147:1037-1044(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-218.

Entry informationi

Entry nameiCD28_MOUSE
AccessioniPrimary (citable) accession number: P31041
Secondary accession number(s): Q6GSH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 3, 2012
Last modified: November 26, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3