Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

T-cell-specific surface glycoprotein CD28

Gene

Cd28

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.By similarity

GO - Molecular functioni

  1. protease binding Source: MGI
  2. SH3/SH2 adaptor activity Source: MGI

GO - Biological processi

  1. apoptotic signaling pathway Source: MGI
  2. immune response Source: InterPro
  3. negative thymic T cell selection Source: MGI
  4. positive regulation of alpha-beta T cell proliferation Source: MGI
  5. positive regulation of inflammatory response to antigenic stimulus Source: MGI
  6. positive regulation of interleukin-2 biosynthetic process Source: UniProtKB
  7. positive regulation of isotype switching to IgG isotypes Source: MGI
  8. positive regulation of mitotic nuclear division Source: UniProtKB
  9. positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
  10. positive regulation of protein kinase B signaling Source: MGI
  11. positive regulation of T cell proliferation Source: UniProtKB
  12. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  13. regulatory T cell differentiation Source: MGI
  14. T cell costimulation Source: MGI
  15. T cell receptor signaling pathway Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_314817. CD28 dependent Vav1 pathway.
REACT_327235. CD28 dependent PI3K/Akt signaling.
REACT_328674. Constitutive PI3K/AKT Signaling in Cancer.
REACT_333474. PIP3 activates AKT signaling.
REACT_334921. CD28 co-stimulation.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell-specific surface glycoprotein CD28
Alternative name(s):
CD_antigen: CD28
Gene namesi
Name:Cd28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:88327. Cd28.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 150131ExtracellularSequence AnalysisAdd
BLAST
Transmembranei151 – 17727HelicalSequence AnalysisAdd
BLAST
Topological domaini178 – 21841CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. external side of plasma membrane Source: MGI
  2. immunological synapse Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919By similarityAdd
BLAST
Chaini20 – 218199T-cell-specific surface glycoprotein CD28PRO_0000014653Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 113By similarity
Disulfide bondi67 ↔ 87By similarity
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence Analysis
Modified residuei187 – 1871PhosphoserineBy similarity
Modified residuei189 – 1891PhosphotyrosineBy similarity
Modified residuei207 – 2071PhosphotyrosineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP31041.

PTM databases

PhosphoSiteiP31041.

Expressioni

Gene expression databases

CleanExiMM_CD28.
GenevestigatoriP31041.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. Interacts with GRB2 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-60858N.
IntActiP31041. 1 interaction.
STRINGi10090.ENSMUSP00000027165.

Structurei

3D structure databases

ProteinModelPortaliP31041.
SMRiP31041. Positions 20-137.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 138110Ig-like V-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG48152.
GeneTreeiENSGT00530000063873.
HOGENOMiHOG000276892.
HOVERGENiHBG004094.
InParanoidiP31041.
KOiK06470.
OMAiNHTDIYF.
OrthoDBiEOG7W6WMJ.
TreeFamiTF335679.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR008093. CD28.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
PRINTSiPR01717. CD28ANTIGEN.
SMARTiSM00409. IG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLRLLFLAL NFFSVQVTEN KILVKQSPLL VVDSNEVSLS CRYSYNLLAK
60 70 80 90 100
EFRASLYKGV NSDVEVCVGN GNFTYQPQFR SNAEFNCDGD FDNETVTFRL
110 120 130 140 150
WNLHVNHTDI YFCKIEFMYP PPYLDNERSN GTIIHIKEKH LCHTQSSPKL
160 170 180 190 200
FWALVVVAGV LFCYGLLVTV ALCVIWTNSR RNRLLQSDYM NMTPRRPGLT
210
RKPYQPYAPA RDFAAYRP
Length:218
Mass (Da):25,243
Last modified:October 2, 2012 - v2
Checksum:iC8E1339DD7E53CA6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti187 – 1893SDY → VTT in AAA37395 (PubMed:2157764).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34563 mRNA. Translation: AAA37395.1.
AL646054 Genomic DNA. Translation: CAI24197.1.
AL672024 Genomic DNA. Translation: CAI26227.1.
CR936842 Genomic DNA. Translation: CAM13249.1.
CH466548 Genomic DNA. Translation: EDL00156.1.
BC064058 mRNA. Translation: AAH64058.1.
M74361 mRNA. Translation: AAA37396.1.
CCDSiCCDS14992.1.
PIRiA43523.
I49584.
RefSeqiNP_031668.3. NM_007642.4.
UniGeneiMm.255003.

Genome annotation databases

EnsembliENSMUST00000027165; ENSMUSP00000027165; ENSMUSG00000026012.
ENSMUST00000193720; ENSMUSP00000141225; ENSMUSG00000102412.
GeneIDi12487.
KEGGimmu:12487.
UCSCiuc007bev.2. mouse.
uc007bew.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34563 mRNA. Translation: AAA37395.1.
AL646054 Genomic DNA. Translation: CAI24197.1.
AL672024 Genomic DNA. Translation: CAI26227.1.
CR936842 Genomic DNA. Translation: CAM13249.1.
CH466548 Genomic DNA. Translation: EDL00156.1.
BC064058 mRNA. Translation: AAH64058.1.
M74361 mRNA. Translation: AAA37396.1.
CCDSiCCDS14992.1.
PIRiA43523.
I49584.
RefSeqiNP_031668.3. NM_007642.4.
UniGeneiMm.255003.

3D structure databases

ProteinModelPortaliP31041.
SMRiP31041. Positions 20-137.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60858N.
IntActiP31041. 1 interaction.
STRINGi10090.ENSMUSP00000027165.

PTM databases

PhosphoSiteiP31041.

Proteomic databases

PRIDEiP31041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027165; ENSMUSP00000027165; ENSMUSG00000026012.
ENSMUST00000193720; ENSMUSP00000141225; ENSMUSG00000102412.
GeneIDi12487.
KEGGimmu:12487.
UCSCiuc007bev.2. mouse.
uc007bew.2. mouse.

Organism-specific databases

CTDi940.
MGIiMGI:88327. Cd28.

Phylogenomic databases

eggNOGiNOG48152.
GeneTreeiENSGT00530000063873.
HOGENOMiHOG000276892.
HOVERGENiHBG004094.
InParanoidiP31041.
KOiK06470.
OMAiNHTDIYF.
OrthoDBiEOG7W6WMJ.
TreeFamiTF335679.

Enzyme and pathway databases

ReactomeiREACT_314817. CD28 dependent Vav1 pathway.
REACT_327235. CD28 dependent PI3K/Akt signaling.
REACT_328674. Constitutive PI3K/AKT Signaling in Cancer.
REACT_333474. PIP3 activates AKT signaling.
REACT_334921. CD28 co-stimulation.

Miscellaneous databases

NextBioi281396.
PROiP31041.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CD28.
GenevestigatoriP31041.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR008093. CD28.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
PRINTSiPR01717. CD28ANTIGEN.
SMARTiSM00409. IG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The murine homologue of the T lymphocyte antigen CD28. Molecular cloning and cell surface expression."
    Gross J.A., St John T., Allison J.P.
    J. Immunol. 144:3201-3210(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Thymus.
  5. "CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequence, message expression, gene structure, and chromosomal location."
    Harper K., Balzano C., Rouvier E., Mattei M.-G., Luciani M.-F., Golstein P.
    J. Immunol. 147:1037-1044(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-218.

Entry informationi

Entry nameiCD28_MOUSE
AccessioniPrimary (citable) accession number: P31041
Secondary accession number(s): Q6GSH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 1993
Last sequence update: October 2, 2012
Last modified: March 31, 2015
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.