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Protein

T-cell-specific surface glycoprotein CD28

Gene

Cd28

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.By similarity

GO - Molecular functioni

  • protease binding Source: MGI
  • protein kinase binding Source: MGI
  • SH3/SH2 adaptor activity Source: MGI

GO - Biological processi

  • apoptotic signaling pathway Source: MGI
  • immune response Source: InterPro
  • negative regulation of gene expression Source: MGI
  • negative thymic T cell selection Source: MGI
  • positive regulation of alpha-beta T cell proliferation Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of inflammatory response to antigenic stimulus Source: MGI
  • positive regulation of interleukin-10 production Source: UniProtKB
  • positive regulation of interleukin-2 biosynthetic process Source: MGI
  • positive regulation of interleukin-4 production Source: UniProtKB
  • positive regulation of isotype switching to IgG isotypes Source: MGI
  • positive regulation of mitotic nuclear division Source: UniProtKB
  • positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
  • positive regulation of protein kinase B signaling Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of regulatory T cell differentiation Source: MGI
  • regulatory T cell differentiation Source: MGI
  • T cell activation Source: MGI
  • T cell costimulation Source: MGI
  • T cell receptor signaling pathway Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-1257604 PIP3 activates AKT signaling
R-MMU-389356 CD28 co-stimulation
R-MMU-389357 CD28 dependent PI3K/Akt signaling
R-MMU-389359 CD28 dependent Vav1 pathway
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell-specific surface glycoprotein CD28
Alternative name(s):
CD_antigen: CD28
Gene namesi
Name:Cd28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:88327 Cd28

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 150ExtracellularSequence analysisAdd BLAST131
Transmembranei151 – 177HelicalSequence analysisAdd BLAST27
Topological domaini178 – 218CytoplasmicSequence analysisAdd BLAST41

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000001465320 – 218T-cell-specific surface glycoprotein CD28Add BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 113By similarity
Disulfide bondi67 ↔ 87By similarity
Glycosylationi72N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi93N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi106N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi130N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei187PhosphoserineBy similarity1
Modified residuei189PhosphotyrosineBy similarity1
Modified residuei207PhosphotyrosineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP31041
PaxDbiP31041
PeptideAtlasiP31041
PRIDEiP31041

PTM databases

iPTMnetiP31041
PhosphoSitePlusiP31041
SwissPalmiP31041

Expressioni

Gene expression databases

BgeeiENSMUSG00000026012
CleanExiMM_CD28
GenevisibleiP31041 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. Interacts with GRB2 (By similarity).By similarity

GO - Molecular functioni

  • protease binding Source: MGI
  • protein kinase binding Source: MGI
  • SH3/SH2 adaptor activity Source: MGI

Protein-protein interaction databases

DIPiDIP-60858N
IntActiP31041, 3 interactors
STRINGi10090.ENSMUSP00000027165

Structurei

Secondary structure

1218
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi185 – 187Combined sources3
Turni214 – 216Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NAENMR-A177-218[»]
ProteinModelPortaliP31041
SMRiP31041
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 138Ig-like V-typeAdd BLAST110

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IXMM Eukaryota
ENOG410Z063 LUCA
GeneTreeiENSGT00530000063873
HOGENOMiHOG000276892
HOVERGENiHBG004094
InParanoidiP31041
KOiK06470
OMAiVNGNYSH
OrthoDBiEOG091G0IGY
TreeFamiTF335679

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR008093 CD28
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF15910 V-set_2, 1 hit
PRINTSiPR01717 CD28ANTIGEN
SUPFAMiSSF48726 SSF48726, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLRLLFLAL NFFSVQVTEN KILVKQSPLL VVDSNEVSLS CRYSYNLLAK
60 70 80 90 100
EFRASLYKGV NSDVEVCVGN GNFTYQPQFR SNAEFNCDGD FDNETVTFRL
110 120 130 140 150
WNLHVNHTDI YFCKIEFMYP PPYLDNERSN GTIIHIKEKH LCHTQSSPKL
160 170 180 190 200
FWALVVVAGV LFCYGLLVTV ALCVIWTNSR RNRLLQSDYM NMTPRRPGLT
210
RKPYQPYAPA RDFAAYRP
Length:218
Mass (Da):25,243
Last modified:October 3, 2012 - v2
Checksum:iC8E1339DD7E53CA6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187 – 189SDY → VTT in AAA37395 (PubMed:2157764).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34563 mRNA Translation: AAA37395.1
AL646054 Genomic DNA Translation: CAI24197.1
AL672024 Genomic DNA Translation: CAI26227.1
CR936842 Genomic DNA Translation: CAM13249.1
CH466548 Genomic DNA Translation: EDL00156.1
BC064058 mRNA Translation: AAH64058.1
M74361 mRNA Translation: AAA37396.1
CCDSiCCDS14992.1
PIRiA43523
I49584
RefSeqiNP_031668.3, NM_007642.4
UniGeneiMm.255003

Genome annotation databases

EnsembliENSMUST00000027165; ENSMUSP00000027165; ENSMUSG00000026012
ENSMUST00000193720; ENSMUSP00000141225; ENSMUSG00000102412
GeneIDi12487
KEGGimmu:12487
UCSCiuc007bev.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCD28_MOUSE
AccessioniPrimary (citable) accession number: P31041
Secondary accession number(s): Q6GSH7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 3, 2012
Last modified: March 28, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health