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P31041

- CD28_MOUSE

UniProt

P31041 - CD28_MOUSE

Protein

T-cell-specific surface glycoprotein CD28

Gene

Cd28

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.By similarity

    GO - Biological processi

    1. apoptotic signaling pathway Source: MGI
    2. immune response Source: InterPro
    3. negative thymic T cell selection Source: MGI
    4. positive regulation of alpha-beta T cell proliferation Source: MGI
    5. positive regulation of inflammatory response to antigenic stimulus Source: MGI
    6. positive regulation of interleukin-2 biosynthetic process Source: UniProtKB
    7. positive regulation of isotype switching to IgG isotypes Source: MGI
    8. positive regulation of mitosis Source: UniProtKB
    9. positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
    10. positive regulation of protein kinase B signaling Source: MGI
    11. positive regulation of T cell proliferation Source: UniProtKB
    12. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    13. regulatory T cell differentiation Source: Ensembl
    14. T cell costimulation Source: MGI
    15. T cell receptor signaling pathway Source: MGI

    Enzyme and pathway databases

    ReactomeiREACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
    REACT_204081. CD28 co-stimulation.
    REACT_226151. CD28 dependent PI3K/Akt signaling.
    REACT_226341. PIP3 activates AKT signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    T-cell-specific surface glycoprotein CD28
    Alternative name(s):
    CD_antigen: CD28
    Gene namesi
    Name:Cd28
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:88327. Cd28.

    Subcellular locationi

    GO - Cellular componenti

    1. external side of plasma membrane Source: MGI
    2. immunological synapse Source: MGI
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919By similarityAdd
    BLAST
    Chaini20 – 218199T-cell-specific surface glycoprotein CD28PRO_0000014653Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi41 ↔ 113By similarity
    Disulfide bondi67 ↔ 87By similarity
    Glycosylationi72 – 721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence Analysis
    Modified residuei187 – 1871PhosphoserineBy similarity
    Modified residuei189 – 1891PhosphotyrosineBy similarity
    Modified residuei207 – 2071PhosphotyrosineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP31041.

    PTM databases

    PhosphoSiteiP31041.

    Expressioni

    Gene expression databases

    CleanExiMM_CD28.
    GenevestigatoriP31041.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. Interacts with GRB2 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-60858N.
    IntActiP31041. 1 interaction.
    STRINGi10090.ENSMUSP00000027165.

    Structurei

    3D structure databases

    ProteinModelPortaliP31041.
    SMRiP31041. Positions 20-137.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 150131ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini178 – 21841CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei151 – 17727HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 138110Ig-like V-typeAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG48152.
    GeneTreeiENSGT00530000063873.
    HOGENOMiHOG000276892.
    HOVERGENiHBG004094.
    InParanoidiP31041.
    KOiK06470.
    OMAiVNGNYSH.
    OrthoDBiEOG7W6WMJ.
    TreeFamiTF335679.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR008093. CD28.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view]
    PfamiPF07686. V-set. 1 hit.
    [Graphical view]
    PRINTSiPR01717. CD28ANTIGEN.
    SMARTiSM00409. IG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31041-1 [UniParc]FASTAAdd to Basket

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    MTLRLLFLAL NFFSVQVTEN KILVKQSPLL VVDSNEVSLS CRYSYNLLAK    50
    EFRASLYKGV NSDVEVCVGN GNFTYQPQFR SNAEFNCDGD FDNETVTFRL 100
    WNLHVNHTDI YFCKIEFMYP PPYLDNERSN GTIIHIKEKH LCHTQSSPKL 150
    FWALVVVAGV LFCYGLLVTV ALCVIWTNSR RNRLLQSDYM NMTPRRPGLT 200
    RKPYQPYAPA RDFAAYRP 218
    Length:218
    Mass (Da):25,243
    Last modified:October 3, 2012 - v2
    Checksum:iC8E1339DD7E53CA6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti187 – 1893SDY → VTT in AAA37395. (PubMed:2157764)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34563 mRNA. Translation: AAA37395.1.
    AL646054 Genomic DNA. Translation: CAI24197.1.
    AL672024 Genomic DNA. Translation: CAI26227.1.
    CR936842 Genomic DNA. Translation: CAM13249.1.
    CH466548 Genomic DNA. Translation: EDL00156.1.
    BC064058 mRNA. Translation: AAH64058.1.
    M74361 mRNA. Translation: AAA37396.1.
    CCDSiCCDS14992.1.
    PIRiA43523.
    I49584.
    RefSeqiNP_031668.3. NM_007642.4.
    UniGeneiMm.255003.

    Genome annotation databases

    EnsembliENSMUST00000027165; ENSMUSP00000027165; ENSMUSG00000026012.
    GeneIDi12487.
    KEGGimmu:12487.
    UCSCiuc007bev.2. mouse.
    uc007bew.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34563 mRNA. Translation: AAA37395.1 .
    AL646054 Genomic DNA. Translation: CAI24197.1 .
    AL672024 Genomic DNA. Translation: CAI26227.1 .
    CR936842 Genomic DNA. Translation: CAM13249.1 .
    CH466548 Genomic DNA. Translation: EDL00156.1 .
    BC064058 mRNA. Translation: AAH64058.1 .
    M74361 mRNA. Translation: AAA37396.1 .
    CCDSi CCDS14992.1.
    PIRi A43523.
    I49584.
    RefSeqi NP_031668.3. NM_007642.4.
    UniGenei Mm.255003.

    3D structure databases

    ProteinModelPortali P31041.
    SMRi P31041. Positions 20-137.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60858N.
    IntActi P31041. 1 interaction.
    STRINGi 10090.ENSMUSP00000027165.

    PTM databases

    PhosphoSitei P31041.

    Proteomic databases

    PRIDEi P31041.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027165 ; ENSMUSP00000027165 ; ENSMUSG00000026012 .
    GeneIDi 12487.
    KEGGi mmu:12487.
    UCSCi uc007bev.2. mouse.
    uc007bew.2. mouse.

    Organism-specific databases

    CTDi 940.
    MGIi MGI:88327. Cd28.

    Phylogenomic databases

    eggNOGi NOG48152.
    GeneTreei ENSGT00530000063873.
    HOGENOMi HOG000276892.
    HOVERGENi HBG004094.
    InParanoidi P31041.
    KOi K06470.
    OMAi VNGNYSH.
    OrthoDBi EOG7W6WMJ.
    TreeFami TF335679.

    Enzyme and pathway databases

    Reactomei REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
    REACT_204081. CD28 co-stimulation.
    REACT_226151. CD28 dependent PI3K/Akt signaling.
    REACT_226341. PIP3 activates AKT signaling.

    Miscellaneous databases

    NextBioi 281396.
    PROi P31041.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_CD28.
    Genevestigatori P31041.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR008093. CD28.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view ]
    Pfami PF07686. V-set. 1 hit.
    [Graphical view ]
    PRINTSi PR01717. CD28ANTIGEN.
    SMARTi SM00409. IG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The murine homologue of the T lymphocyte antigen CD28. Molecular cloning and cell surface expression."
      Gross J.A., St John T., Allison J.P.
      J. Immunol. 144:3201-3210(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Thymus.
    5. "CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequence, message expression, gene structure, and chromosomal location."
      Harper K., Balzano C., Rouvier E., Mattei M.-G., Luciani M.-F., Golstein P.
      J. Immunol. 147:1037-1044(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-218.

    Entry informationi

    Entry nameiCD28_MOUSE
    AccessioniPrimary (citable) accession number: P31041
    Secondary accession number(s): Q6GSH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3