Reviewed, UniProtKB/Swiss-Prot P31039 (DHSA_BOVIN)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
    EC=1.3.5.1
Alternative name(s):
    Flavoprotein subunit of complex II
      Short name=Fp

Gene names

Name:	SDHA
Synonyms:	SDH2, SDHFP1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	665 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	FAD.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 44	44	Mitochondrion
	Chain	45 – 665	621	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial		PRO_0000010334
Regions
	Nucleotide binding	92 – 107	16	FAD By similarity
Sites
	Active site	341	1	Proton acceptor By similarity
	Binding site	297	1	Substrate By similarity
	Binding site	309	1	Substrate By similarity
	Binding site	408	1	Substrate By similarity
	Binding site	452	1	Substrate By similarity
Amino acid modifications
	Modified residue	100	1	Tele-8alpha-FAD histidine By similarity
	Modified residue	180	1	N6-acetyllysine By similarity
	Modified residue	253	1	Phosphothreonine By similarity
	Modified residue	486	1	N6-acetyllysine By similarity
	Modified residue	499	1	N6-acetyllysine By similarity
	Modified residue	539	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	242 – 253	12	DGSIH…RARNT → ERVHPPHQGQEH Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P31039-1 [UniParc]. Last modified August 16, 2004. Version 3. Checksum: FE51160F248D51BC	FASTA	665	72,944
	10 20 30 40 50 60 MSGVAAVSRL WRARRLALTC TKWSAAWQTG TRSFHFTVDG NKRSSAKVSD AISAQYPVVD 70 80 90 100 110 120 HEFDAVVVGA GGAGLRAAFG LSEAGFNTAC VTKLFPTRSH TVAAQGGINA ALGNMEEDNW 130 140 150 160 170 180 RWHFYDTVKG SDWLGDQDAI HYMTEQAPAS VVELENYGMP FSRTEDGKIY QRAFGGQSLK 190 200 210 220 230 240 FGKGGQAHRC CCVADRTGHS LLHTLYGRSL RYDTSYFVEY FALDLLMESG ECRGVIALCI 250 260 270 280 290 300 EDGSIHRIRA RNTVIATGGY GRTYFSCTSA HTSTGDGTAM VTRAGLPCQD LEFVQFHPTG 310 320 330 340 350 360 IYGAGCLITE GCRGEGGILI NSQGERFMER YAPVAKDLAS RDVVSRSMTL EIREGRGCGP 370 380 390 400 410 420 EKDHVYLQLH HLPPAQLAMR LPGISETAMI FAGVDVTKEP IPVLPTVHYN MGGIPTNYKG 430 440 450 460 470 480 QVLRHVNGQD QGVPGLYACG EAACASVHGA NRLGANSLLD LVVFGRACAL SIAESCRPGD 490 500 510 520 530 540 KVPSIKPNAG EESVMNLDKL RFANGSIRTS ELRLNMQKSM QSHAAVFRVG SVLQEGCEKI 550 560 570 580 590 600 SSLYGDLRHL KTFDRGMVWN TDLVETLELQ NLMLCALQTI YGAEARKESR GGPRREDFKE 610 620 630 640 650 660 RVDEYDYSKP IQGQQKKPFE QHWRKHTLSY VDIKTGKVTL EYRPVIDRTL NETDCATVPP AIGSY

References

[1]	"The sequence of the flavoprotein subunit of bovine heart succinate dehydrogenase." Birch-MacHin M.A., Farnsworth L., Ackrell B.A.C., Cochran B., Jackson S., Bindoff L.A., Aitken A., Diamond A.G., Turnbull D.M. J. Biol. Chem. 267:11553-11558(1992) [PubMed: 1375942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Heart.
[2]	"The cDNA sequence of the flavoprotein subunit of human heart succinate dehydrogenase." Morris A.A.M., Farnsworth L., Ackrell B.A.C., Turnbull D.M., Birch-MacHin M.A. Biochim. Biophys. Acta 1185:125-128(1994) [PubMed: 8142412] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Mapping of the SDHA locus to bovine chromosome 20." Sonstegard T.S., Kappes S.M. Anim. Genet. 30:473-473(1999) [PubMed: 10612251] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 153-253. Strain: Hereford X Nelore.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60879 mRNA. Translation: AAA30758.1. Sequence problems. AF139922 Genomic DNA. Translation: AAD38150.1.
IPI	IPI00686177.
PIR	A42792.
RefSeq	NP_776603.1.
UniGene	Bt.24449
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P10444.
SMR	P31039. Positions 55-665.
ModBase	Search...
Genome annotation databases
GeneID	281480.
KEGG	bta:281480.
Phylogenomic databases
HOVERGEN	P31039.
Enzyme and pathway databases
BRENDA	1.3.5.1. 251.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR011281. Succ_DH_flav_su_fwd. IPR014006. Succ_Dhase_frdA_Gneg. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_BOVIN

Accession

Primary (citable) accession number: P31039
Secondary accession number(s): Q9TUY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)