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P31039

- SDHA_BOVIN

UniProt

P31039 - SDHA_BOVIN

Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gene

SDHA

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 3 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor By similarity.By similarity

    Catalytic activityi

    Succinate + a quinone = fumarate + a quinol.

    Cofactori

    FAD.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei276 – 2761FADBy similarity
    Binding sitei297 – 2971SubstrateBy similarity
    Binding sitei309 – 3091SubstrateBy similarity
    Active sitei341 – 3411Proton acceptorBy similarity
    Binding sitei408 – 4081SubstrateBy similarity
    Binding sitei441 – 4411FADBy similarity
    Binding sitei452 – 4521SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi69 – 746FADBy similarity
    Nucleotide bindingi92 – 10716FADBy similarityAdd
    BLAST
    Nucleotide bindingi457 – 4582FADBy similarity

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. electron transport chain Source: InterPro
    2. oxidation-reduction process Source: AgBase
    3. tricarboxylic acid cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport, Tricarboxylic acid cycle

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    ReactomeiREACT_223064. Respiratory electron transport.
    UniPathwayiUPA00223; UER01006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (EC:1.3.5.1)
    Alternative name(s):
    Flavoprotein subunit of complex II
    Short name:
    Fp
    Gene namesi
    Name:SDHA
    Synonyms:SDH2, SDHFP1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB
    2. mitochondrial respiratory chain complex II Source: UniProtKB
    3. mitochondrion Source: AgBase

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4444MitochondrionAdd
    BLAST
    Chaini45 – 665621Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrialPRO_0000010334Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei100 – 1001Tele-8alpha-FAD histidineBy similarity
    Modified residuei168 – 1681N6-acetyllysineBy similarity
    Modified residuei180 – 1801N6-acetyllysine; alternateBy similarity
    Modified residuei180 – 1801N6-succinyllysine; alternateBy similarity
    Modified residuei183 – 1831N6-acetyllysineBy similarity
    Modified residuei216 – 2161Phosphotyrosine; by SRCBy similarity
    Modified residuei336 – 3361N6-acetyllysine; alternateBy similarity
    Modified residuei336 – 3361N6-succinyllysine; alternateBy similarity
    Modified residuei481 – 4811N6-acetyllysineBy similarity
    Modified residuei486 – 4861N6-acetyllysine; alternateBy similarity
    Modified residuei486 – 4861N6-succinyllysine; alternateBy similarity
    Modified residuei499 – 4991N6-acetyllysine; alternateBy similarity
    Modified residuei499 – 4991N6-succinyllysine; alternateBy similarity
    Modified residuei518 – 5181N6-acetyllysineBy similarity
    Modified residuei539 – 5391N6-acetyllysine; alternateBy similarity
    Modified residuei539 – 5391N6-succinyllysine; alternateBy similarity
    Modified residuei551 – 5511N6-acetyllysineBy similarity
    Modified residuei599 – 5991N6-acetyllysineBy similarity
    Modified residuei609 – 6091N6-acetyllysineBy similarity
    Modified residuei616 – 6161N6-succinyllysineBy similarity
    Modified residuei625 – 6251N6-acetyllysineBy similarity
    Modified residuei634 – 6341N6-acetyllysineBy similarity
    Modified residuei637 – 6371N6-acetyllysineBy similarity

    Post-translational modificationi

    Phosphorylation at Tyr-216 is important for efficient electron transfer in complex II and the prevention of ROS generation.By similarity
    Deacetylated by SIRT3.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiP31039.

    Interactioni

    Subunit structurei

    Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD. Interacts with SDHAF2/SDH5; interaction is required for FAD attachment By similarity. Interacts with TRAP1 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP31039. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP31039.
    SMRiP31039. Positions 53-665.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOVERGENiHBG001461.
    KOiK00234.

    Family and domain databases

    Gene3Di1.20.58.100. 1 hit.
    3.90.700.10. 1 hit.
    InterProiIPR003953. FAD_bind_dom.
    IPR003952. FRD_SDH_FAD_BS.
    IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    IPR011281. Succ_DH_flav_su_fwd.
    IPR014006. Succ_Dhase_FrdA_Gneg.
    [Graphical view]
    PfamiPF00890. FAD_binding_2. 1 hit.
    PF02910. Succ_DH_flav_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF46977. SSF46977. 1 hit.
    SSF56425. SSF56425. 1 hit.
    TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
    TIGR01812. sdhA_frdA_Gneg. 1 hit.
    PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31039-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGVAAVSRL WRARRLALTC TKWSAAWQTG TRSFHFTVDG NKRSSAKVSD    50
    AISAQYPVVD HEFDAVVVGA GGAGLRAAFG LSEAGFNTAC VTKLFPTRSH 100
    TVAAQGGINA ALGNMEEDNW RWHFYDTVKG SDWLGDQDAI HYMTEQAPAS 150
    VVELENYGMP FSRTEDGKIY QRAFGGQSLK FGKGGQAHRC CCVADRTGHS 200
    LLHTLYGRSL RYDTSYFVEY FALDLLMESG ECRGVIALCI EDGSIHRIRA 250
    RNTVIATGGY GRTYFSCTSA HTSTGDGTAM VTRAGLPCQD LEFVQFHPTG 300
    IYGAGCLITE GCRGEGGILI NSQGERFMER YAPVAKDLAS RDVVSRSMTL 350
    EIREGRGCGP EKDHVYLQLH HLPPAQLAMR LPGISETAMI FAGVDVTKEP 400
    IPVLPTVHYN MGGIPTNYKG QVLRHVNGQD QGVPGLYACG EAACASVHGA 450
    NRLGANSLLD LVVFGRACAL SIAESCRPGD KVPSIKPNAG EESVMNLDKL 500
    RFANGSIRTS ELRLNMQKSM QSHAAVFRVG SVLQEGCEKI SSLYGDLRHL 550
    KTFDRGMVWN TDLVETLELQ NLMLCALQTI YGAEARKESR GGPRREDFKE 600
    RVDEYDYSKP IQGQQKKPFE QHWRKHTLSY VDIKTGKVTL EYRPVIDRTL 650
    NETDCATVPP AIGSY 665
    Length:665
    Mass (Da):72,944
    Last modified:August 16, 2004 - v3
    Checksum:iFE51160F248D51BC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti242 – 25312DGSIH…RARNT → ERVHPPHQGQEH in AAD38150. (PubMed:10612251)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60879 mRNA. Translation: AAA30758.1. Sequence problems.
    AF139922 Genomic DNA. Translation: AAD38150.1.
    PIRiA42792.
    RefSeqiNP_776603.1. NM_174178.2.
    UniGeneiBt.24449.

    Genome annotation databases

    GeneIDi281480.
    KEGGibta:281480.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60879 mRNA. Translation: AAA30758.1 . Sequence problems.
    AF139922 Genomic DNA. Translation: AAD38150.1 .
    PIRi A42792.
    RefSeqi NP_776603.1. NM_174178.2.
    UniGenei Bt.24449.

    3D structure databases

    ProteinModelPortali P31039.
    SMRi P31039. Positions 53-665.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P31039. 2 interactions.

    Proteomic databases

    PRIDEi P31039.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281480.
    KEGGi bta:281480.

    Organism-specific databases

    CTDi 6389.

    Phylogenomic databases

    HOVERGENi HBG001461.
    KOi K00234.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER01006 .
    Reactomei REACT_223064. Respiratory electron transport.

    Miscellaneous databases

    NextBioi 20805455.

    Family and domain databases

    Gene3Di 1.20.58.100. 1 hit.
    3.90.700.10. 1 hit.
    InterProi IPR003953. FAD_bind_dom.
    IPR003952. FRD_SDH_FAD_BS.
    IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    IPR011281. Succ_DH_flav_su_fwd.
    IPR014006. Succ_Dhase_FrdA_Gneg.
    [Graphical view ]
    Pfami PF00890. FAD_binding_2. 1 hit.
    PF02910. Succ_DH_flav_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46977. SSF46977. 1 hit.
    SSF56425. SSF56425. 1 hit.
    TIGRFAMsi TIGR01816. sdhA_forward. 1 hit.
    TIGR01812. sdhA_frdA_Gneg. 1 hit.
    PROSITEi PS00504. FRD_SDH_FAD_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of the flavoprotein subunit of bovine heart succinate dehydrogenase."
      Birch-MacHin M.A., Farnsworth L., Ackrell B.A.C., Cochran B., Jackson S., Bindoff L.A., Aitken A., Diamond A.G., Turnbull D.M.
      J. Biol. Chem. 267:11553-11558(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Heart.
    2. "The cDNA sequence of the flavoprotein subunit of human heart succinate dehydrogenase."
      Morris A.A.M., Farnsworth L., Ackrell B.A.C., Turnbull D.M., Birch-MacHin M.A.
      Biochim. Biophys. Acta 1185:125-128(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    3. "Mapping of the SDHA locus to bovine chromosome 20."
      Sonstegard T.S., Kappes S.M.
      Anim. Genet. 30:473-473(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 153-253.
      Strain: Hereford X Nelore.

    Entry informationi

    Entry nameiSDHA_BOVIN
    AccessioniPrimary (citable) accession number: P31039
    Secondary accession number(s): Q9TUY8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 125 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3