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P31039 (SDHA_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

EC=1.3.5.1
Alternative name(s):
Flavoprotein subunit of complex II
Short name=Fp
Gene names
Name:SDHA
Synonyms:SDH2, SDHFP1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length665 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor By similarity.

Catalytic activity

Succinate + a quinone = fumarate + a quinol.

Cofactor

FAD.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.

Subunit structure

Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD. Interacts with SDHAF2/SDH5; interaction is required for FAD attachment By similarity. Interacts with TRAP1 By similarity.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

Post-translational modification

Phosphorylation at Tyr-216 is important for efficient electron transfer in complex II and the prevention of ROS generation By similarity.

Deacetylated by SIRT3 By similarity.

Sequence similarities

Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4444Mitochondrion
Chain45 – 665621Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
PRO_0000010334

Regions

Nucleotide binding69 – 746FAD By similarity
Nucleotide binding92 – 10716FAD By similarity
Nucleotide binding457 – 4582FAD By similarity

Sites

Active site3411Proton acceptor By similarity
Binding site2761FAD By similarity
Binding site2971Substrate By similarity
Binding site3091Substrate By similarity
Binding site4081Substrate By similarity
Binding site4411FAD By similarity
Binding site4521Substrate By similarity

Amino acid modifications

Modified residue1001Tele-8alpha-FAD histidine By similarity
Modified residue1681N6-acetyllysine By similarity
Modified residue1801N6-acetyllysine; alternate By similarity
Modified residue1801N6-succinyllysine; alternate By similarity
Modified residue1831N6-acetyllysine By similarity
Modified residue2161Phosphotyrosine; by SRC By similarity
Modified residue3361N6-acetyllysine; alternate By similarity
Modified residue3361N6-succinyllysine; alternate By similarity
Modified residue4811N6-acetyllysine By similarity
Modified residue4861N6-acetyllysine; alternate By similarity
Modified residue4861N6-succinyllysine; alternate By similarity
Modified residue4991N6-acetyllysine; alternate By similarity
Modified residue4991N6-succinyllysine; alternate By similarity
Modified residue5181N6-acetyllysine By similarity
Modified residue5391N6-acetyllysine; alternate By similarity
Modified residue5391N6-succinyllysine; alternate By similarity
Modified residue5511N6-acetyllysine By similarity
Modified residue5991N6-acetyllysine By similarity
Modified residue6091N6-acetyllysine By similarity
Modified residue6161N6-succinyllysine By similarity
Modified residue6251N6-acetyllysine By similarity
Modified residue6341N6-acetyllysine By similarity
Modified residue6371N6-acetyllysine By similarity

Experimental info

Sequence conflict242 – 25312DGSIH…RARNT → ERVHPPHQGQEH in AAD38150. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P31039 [UniParc].

Last modified August 16, 2004. Version 3.
Checksum: FE51160F248D51BC

FASTA66572,944
        10         20         30         40         50         60 
MSGVAAVSRL WRARRLALTC TKWSAAWQTG TRSFHFTVDG NKRSSAKVSD AISAQYPVVD 

        70         80         90        100        110        120 
HEFDAVVVGA GGAGLRAAFG LSEAGFNTAC VTKLFPTRSH TVAAQGGINA ALGNMEEDNW 

       130        140        150        160        170        180 
RWHFYDTVKG SDWLGDQDAI HYMTEQAPAS VVELENYGMP FSRTEDGKIY QRAFGGQSLK 

       190        200        210        220        230        240 
FGKGGQAHRC CCVADRTGHS LLHTLYGRSL RYDTSYFVEY FALDLLMESG ECRGVIALCI 

       250        260        270        280        290        300 
EDGSIHRIRA RNTVIATGGY GRTYFSCTSA HTSTGDGTAM VTRAGLPCQD LEFVQFHPTG 

       310        320        330        340        350        360 
IYGAGCLITE GCRGEGGILI NSQGERFMER YAPVAKDLAS RDVVSRSMTL EIREGRGCGP 

       370        380        390        400        410        420 
EKDHVYLQLH HLPPAQLAMR LPGISETAMI FAGVDVTKEP IPVLPTVHYN MGGIPTNYKG 

       430        440        450        460        470        480 
QVLRHVNGQD QGVPGLYACG EAACASVHGA NRLGANSLLD LVVFGRACAL SIAESCRPGD 

       490        500        510        520        530        540 
KVPSIKPNAG EESVMNLDKL RFANGSIRTS ELRLNMQKSM QSHAAVFRVG SVLQEGCEKI 

       550        560        570        580        590        600 
SSLYGDLRHL KTFDRGMVWN TDLVETLELQ NLMLCALQTI YGAEARKESR GGPRREDFKE 

       610        620        630        640        650        660 
RVDEYDYSKP IQGQQKKPFE QHWRKHTLSY VDIKTGKVTL EYRPVIDRTL NETDCATVPP 


AIGSY 

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References

[1]"The sequence of the flavoprotein subunit of bovine heart succinate dehydrogenase."
Birch-MacHin M.A., Farnsworth L., Ackrell B.A.C., Cochran B., Jackson S., Bindoff L.A., Aitken A., Diamond A.G., Turnbull D.M.
J. Biol. Chem. 267:11553-11558(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Heart.
[2]"The cDNA sequence of the flavoprotein subunit of human heart succinate dehydrogenase."
Morris A.A.M., Farnsworth L., Ackrell B.A.C., Turnbull D.M., Birch-MacHin M.A.
Biochim. Biophys. Acta 1185:125-128(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"Mapping of the SDHA locus to bovine chromosome 20."
Sonstegard T.S., Kappes S.M.
Anim. Genet. 30:473-473(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 153-253.
Strain: Hereford X Nelore.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60879 mRNA. Translation: AAA30758.1. Sequence problems.
AF139922 Genomic DNA. Translation: AAD38150.1.
PIRA42792.
RefSeqNP_776603.1. NM_174178.2.
UniGeneBt.24449.

3D structure databases

ProteinModelPortalP31039.
SMRP31039. Positions 53-665.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP31039. 2 interactions.

Proteomic databases

PRIDEP31039.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID281480.
KEGGbta:281480.

Organism-specific databases

CTD6389.

Phylogenomic databases

HOVERGENHBG001461.
KOK00234.

Enzyme and pathway databases

UniPathwayUPA00223; UER01006.

Family and domain databases

Gene3D1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProIPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805455.

Entry information

Entry nameSDHA_BOVIN
AccessionPrimary (citable) accession number: P31039
Secondary accession number(s): Q9TUY8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways