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Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gene

SDHA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor (By similarity).By similarity

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei276 – 2761FADBy similarity
Binding sitei297 – 2971SubstrateBy similarity
Binding sitei309 – 3091SubstrateBy similarity
Active sitei341 – 3411Proton acceptorBy similarity
Binding sitei408 – 4081SubstrateBy similarity
Binding sitei441 – 4411FADBy similarity
Binding sitei452 – 4521SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi69 – 746FADBy similarity
Nucleotide bindingi92 – 10716FADBy similarityAdd
BLAST
Nucleotide bindingi457 – 4582FADBy similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. electron transport chain Source: InterPro
  2. oxidation-reduction process Source: AgBase
  3. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiREACT_281214. Respiratory electron transport.
UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Flavoprotein subunit of complex II
Short name:
Fp
Gene namesi
Name:SDHA
Synonyms:SDH2, SDHFP1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB
  2. mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone) Source: UniProtKB
  3. mitochondrion Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4444MitochondrionAdd
BLAST
Chaini45 – 665621Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrialPRO_0000010334Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei100 – 1001Tele-8alpha-FAD histidineBy similarity
Modified residuei168 – 1681N6-acetyllysineBy similarity
Modified residuei180 – 1801N6-acetyllysine; alternateBy similarity
Modified residuei180 – 1801N6-succinyllysine; alternateBy similarity
Modified residuei183 – 1831N6-acetyllysineBy similarity
Modified residuei216 – 2161Phosphotyrosine; by SRCBy similarity
Modified residuei336 – 3361N6-acetyllysine; alternateBy similarity
Modified residuei336 – 3361N6-succinyllysine; alternateBy similarity
Modified residuei481 – 4811N6-acetyllysineBy similarity
Modified residuei486 – 4861N6-acetyllysine; alternateBy similarity
Modified residuei486 – 4861N6-succinyllysine; alternateBy similarity
Modified residuei499 – 4991N6-acetyllysine; alternateBy similarity
Modified residuei499 – 4991N6-succinyllysine; alternateBy similarity
Modified residuei518 – 5181N6-acetyllysineBy similarity
Modified residuei539 – 5391N6-acetyllysine; alternateBy similarity
Modified residuei539 – 5391N6-succinyllysine; alternateBy similarity
Modified residuei551 – 5511N6-acetyllysineBy similarity
Modified residuei599 – 5991N6-acetyllysineBy similarity
Modified residuei609 – 6091N6-acetyllysineBy similarity
Modified residuei616 – 6161N6-succinyllysineBy similarity
Modified residuei625 – 6251N6-acetyllysineBy similarity
Modified residuei634 – 6341N6-acetyllysineBy similarity
Modified residuei637 – 6371N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylation at Tyr-216 is important for efficient electron transfer in complex II and the prevention of ROS generation.By similarity
Deacetylated by SIRT3.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP31039.

Interactioni

Subunit structurei

Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD. Interacts with SDHAF2/SDH5; interaction is required for FAD attachment (By similarity). Interacts with TRAP1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP31039. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP31039.
SMRiP31039. Positions 53-665.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG001461.
InParanoidiP31039.
KOiK00234.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31039-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGVAAVSRL WRARRLALTC TKWSAAWQTG TRSFHFTVDG NKRSSAKVSD
60 70 80 90 100
AISAQYPVVD HEFDAVVVGA GGAGLRAAFG LSEAGFNTAC VTKLFPTRSH
110 120 130 140 150
TVAAQGGINA ALGNMEEDNW RWHFYDTVKG SDWLGDQDAI HYMTEQAPAS
160 170 180 190 200
VVELENYGMP FSRTEDGKIY QRAFGGQSLK FGKGGQAHRC CCVADRTGHS
210 220 230 240 250
LLHTLYGRSL RYDTSYFVEY FALDLLMESG ECRGVIALCI EDGSIHRIRA
260 270 280 290 300
RNTVIATGGY GRTYFSCTSA HTSTGDGTAM VTRAGLPCQD LEFVQFHPTG
310 320 330 340 350
IYGAGCLITE GCRGEGGILI NSQGERFMER YAPVAKDLAS RDVVSRSMTL
360 370 380 390 400
EIREGRGCGP EKDHVYLQLH HLPPAQLAMR LPGISETAMI FAGVDVTKEP
410 420 430 440 450
IPVLPTVHYN MGGIPTNYKG QVLRHVNGQD QGVPGLYACG EAACASVHGA
460 470 480 490 500
NRLGANSLLD LVVFGRACAL SIAESCRPGD KVPSIKPNAG EESVMNLDKL
510 520 530 540 550
RFANGSIRTS ELRLNMQKSM QSHAAVFRVG SVLQEGCEKI SSLYGDLRHL
560 570 580 590 600
KTFDRGMVWN TDLVETLELQ NLMLCALQTI YGAEARKESR GGPRREDFKE
610 620 630 640 650
RVDEYDYSKP IQGQQKKPFE QHWRKHTLSY VDIKTGKVTL EYRPVIDRTL
660
NETDCATVPP AIGSY
Length:665
Mass (Da):72,944
Last modified:August 16, 2004 - v3
Checksum:iFE51160F248D51BC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti242 – 25312DGSIH…RARNT → ERVHPPHQGQEH in AAD38150 (PubMed:10612251).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60879 mRNA. Translation: AAA30758.1. Sequence problems.
AF139922 Genomic DNA. Translation: AAD38150.1.
PIRiA42792.
RefSeqiNP_776603.1. NM_174178.2.
UniGeneiBt.24449.

Genome annotation databases

GeneIDi281480.
KEGGibta:281480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60879 mRNA. Translation: AAA30758.1. Sequence problems.
AF139922 Genomic DNA. Translation: AAD38150.1.
PIRiA42792.
RefSeqiNP_776603.1. NM_174178.2.
UniGeneiBt.24449.

3D structure databases

ProteinModelPortaliP31039.
SMRiP31039. Positions 53-665.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP31039. 2 interactions.

Proteomic databases

PRIDEiP31039.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281480.
KEGGibta:281480.

Organism-specific databases

CTDi6389.

Phylogenomic databases

HOVERGENiHBG001461.
InParanoidiP31039.
KOiK00234.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.
ReactomeiREACT_281214. Respiratory electron transport.

Miscellaneous databases

NextBioi20805455.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The sequence of the flavoprotein subunit of bovine heart succinate dehydrogenase."
    Birch-MacHin M.A., Farnsworth L., Ackrell B.A.C., Cochran B., Jackson S., Bindoff L.A., Aitken A., Diamond A.G., Turnbull D.M.
    J. Biol. Chem. 267:11553-11558(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Heart.
  2. "The cDNA sequence of the flavoprotein subunit of human heart succinate dehydrogenase."
    Morris A.A.M., Farnsworth L., Ackrell B.A.C., Turnbull D.M., Birch-MacHin M.A.
    Biochim. Biophys. Acta 1185:125-128(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. "Mapping of the SDHA locus to bovine chromosome 20."
    Sonstegard T.S., Kappes S.M.
    Anim. Genet. 30:473-473(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 153-253.
    Strain: Hereford X Nelore.

Entry informationi

Entry nameiSDHA_BOVIN
AccessioniPrimary (citable) accession number: P31039
Secondary accession number(s): Q9TUY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: August 16, 2004
Last modified: April 1, 2015
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.