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Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gene

SDHA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (Probable). Can act as a tumor suppressor (By similarity).By similarity1 Publication

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.1 Publication

Cofactori

FADBy similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (eukaryal route).1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (eukaryal route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei276FADBy similarity1
Binding sitei297SubstrateBy similarity1
Binding sitei309SubstrateBy similarity1
Active sitei341Proton acceptorBy similarity1
Binding sitei408SubstrateBy similarity1
Binding sitei441FADBy similarity1
Binding sitei452SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi69 – 74FADBy similarity6
Nucleotide bindingi92 – 107FADBy similarityAdd BLAST16
Nucleotide bindingi457 – 458FADBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (EC:1.3.5.11 Publication)
Alternative name(s):
Flavoprotein subunit of complex II
Short name:
Fp
Gene namesi
Name:SDHA
Synonyms:SDH2, SDHFP1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43MitochondrionBy similarityAdd BLAST43
ChainiPRO_000001033444 – 665Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrialAdd BLAST622

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei100Tele-8alpha-FAD histidineBy similarity1
Modified residuei168N6-acetyllysineBy similarity1
Modified residuei180N6-acetyllysine; alternateBy similarity1
Modified residuei180N6-succinyllysine; alternateBy similarity1
Modified residuei183N6-acetyllysineBy similarity1
Modified residuei216Phosphotyrosine; by SRCBy similarity1
Modified residuei336N6-acetyllysine; alternateBy similarity1
Modified residuei336N6-succinyllysine; alternateBy similarity1
Modified residuei481N6-acetyllysineBy similarity1
Modified residuei486N6-acetyllysine; alternateBy similarity1
Modified residuei486N6-succinyllysine; alternateBy similarity1
Modified residuei499N6-acetyllysine; alternateBy similarity1
Modified residuei499N6-succinyllysine; alternateBy similarity1
Modified residuei518N6-acetyllysineBy similarity1
Modified residuei539N6-acetyllysine; alternateBy similarity1
Modified residuei539N6-succinyllysine; alternateBy similarity1
Modified residuei551N6-acetyllysineBy similarity1
Modified residuei599N6-acetyllysineBy similarity1
Modified residuei609N6-acetyllysineBy similarity1
Modified residuei616N6-succinyllysineBy similarity1
Modified residuei625N6-acetyllysineBy similarity1
Modified residuei634N6-acetyllysineBy similarity1
Modified residuei637N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylation at Tyr-216 is important for efficient electron transfer in complex II and the prevention of ROS generation.By similarity
Acetylated. Deacetylated by SIRT3.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP31039.
PeptideAtlasiP31039.
PRIDEiP31039.

Interactioni

Subunit structurei

Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD (By similarity). Interacts with SDHAF2/SDH5; interaction is required for FAD attachment (By similarity). Interacts with TRAP1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP31039. 2 interactors.
STRINGi9913.ENSBTAP00000054495.

Structurei

3D structure databases

ProteinModelPortaliP31039.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2403. Eukaryota.
COG1053. LUCA.
HOVERGENiHBG001461.
InParanoidiP31039.
KOiK00234.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.50.50.60. 2 hits.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD-binding_2.
IPR023753. FAD/NAD-binding_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR030664. SdhA/FrdA/AprA.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000171. SDHA_APRA_LASPO. 1 hit.
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF51905. SSF51905. 2 hits.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31039-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGVAAVSRL WRARRLALTC TKWSAAWQTG TRSFHFTVDG NKRSSAKVSD
60 70 80 90 100
AISAQYPVVD HEFDAVVVGA GGAGLRAAFG LSEAGFNTAC VTKLFPTRSH
110 120 130 140 150
TVAAQGGINA ALGNMEEDNW RWHFYDTVKG SDWLGDQDAI HYMTEQAPAS
160 170 180 190 200
VVELENYGMP FSRTEDGKIY QRAFGGQSLK FGKGGQAHRC CCVADRTGHS
210 220 230 240 250
LLHTLYGRSL RYDTSYFVEY FALDLLMESG ECRGVIALCI EDGSIHRIRA
260 270 280 290 300
RNTVIATGGY GRTYFSCTSA HTSTGDGTAM VTRAGLPCQD LEFVQFHPTG
310 320 330 340 350
IYGAGCLITE GCRGEGGILI NSQGERFMER YAPVAKDLAS RDVVSRSMTL
360 370 380 390 400
EIREGRGCGP EKDHVYLQLH HLPPAQLAMR LPGISETAMI FAGVDVTKEP
410 420 430 440 450
IPVLPTVHYN MGGIPTNYKG QVLRHVNGQD QGVPGLYACG EAACASVHGA
460 470 480 490 500
NRLGANSLLD LVVFGRACAL SIAESCRPGD KVPSIKPNAG EESVMNLDKL
510 520 530 540 550
RFANGSIRTS ELRLNMQKSM QSHAAVFRVG SVLQEGCEKI SSLYGDLRHL
560 570 580 590 600
KTFDRGMVWN TDLVETLELQ NLMLCALQTI YGAEARKESR GGPRREDFKE
610 620 630 640 650
RVDEYDYSKP IQGQQKKPFE QHWRKHTLSY VDIKTGKVTL EYRPVIDRTL
660
NETDCATVPP AIGSY
Length:665
Mass (Da):72,944
Last modified:August 16, 2004 - v3
Checksum:iFE51160F248D51BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti242 – 253DGSIH…RARNT → ERVHPPHQGQEH in AAD38150 (PubMed:10612251).CuratedAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60879 mRNA. Translation: AAA30758.1. Sequence problems.
AF139922 Genomic DNA. Translation: AAD38150.1.
PIRiA42792.
RefSeqiNP_776603.1. NM_174178.2.
UniGeneiBt.24449.

Genome annotation databases

GeneIDi281480.
KEGGibta:281480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60879 mRNA. Translation: AAA30758.1. Sequence problems.
AF139922 Genomic DNA. Translation: AAD38150.1.
PIRiA42792.
RefSeqiNP_776603.1. NM_174178.2.
UniGeneiBt.24449.

3D structure databases

ProteinModelPortaliP31039.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP31039. 2 interactors.
STRINGi9913.ENSBTAP00000054495.

Proteomic databases

PaxDbiP31039.
PeptideAtlasiP31039.
PRIDEiP31039.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281480.
KEGGibta:281480.

Organism-specific databases

CTDi6389.

Phylogenomic databases

eggNOGiKOG2403. Eukaryota.
COG1053. LUCA.
HOVERGENiHBG001461.
InParanoidiP31039.
KOiK00234.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.
ReactomeiR-BTA-611105. Respiratory electron transport.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.50.50.60. 2 hits.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD-binding_2.
IPR023753. FAD/NAD-binding_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR030664. SdhA/FrdA/AprA.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000171. SDHA_APRA_LASPO. 1 hit.
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF51905. SSF51905. 2 hits.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSDHA_BOVIN
AccessioniPrimary (citable) accession number: P31039
Secondary accession number(s): Q9TUY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: August 16, 2004
Last modified: November 30, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.