P31038 (DHSA_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinate dehydrogenase flavoprotein subunit
EC=1.3.99.1

Gene names

Name:	sdhA
Ordered Locus Names:	RP128

Organism

Rickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia prowazekii

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Cofactor	FAD.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and a hydrophobic anchor protein.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Succinate dehydrogenase flavoprotein subunit		PRO_0000158657
Regions
	Nucleotide binding	18 – 23	6	FAD By similarity
	Nucleotide binding	41 – 56	16	FAD By similarity
	Nucleotide binding	407 – 408	2	FAD By similarity
Sites
	Active site	290	1	Proton acceptor By similarity
	Binding site	225	1	FAD By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	258	1	Substrate By similarity
	Binding site	357	1	Substrate By similarity
	Binding site	391	1	FAD By similarity
	Binding site	402	1	Substrate By similarity
Amino acid modifications
	Modified residue	49	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31038 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: E5CAA5DFE9426EC4	FASTA	596	65,445
	10 20 30 40 50 60 MTKAYNIIHH KFDVVVVGAG GAGLRSAFGM AKEGLNTACI TKIFPTRSHT VAAQGGISAA 70 80 90 100 110 120 LGNMGEDDWR WHMYDTVKGS DWLGDQDAIE YMCKNAPDAI LELEHYGVPF SRTVDGKIYQ 130 140 150 160 170 180 RPFGGMTTEY GKGKAAQRTC AAADRTGHAI LHTLYQQSLK HKVQFFIEYF AIDLLMEDGE 190 200 210 220 230 240 CRGVVAWNLD DGSLHCFRAH NVVLATGGYG RAYFSATSAH TCTGDGGGMV IRAGLPLQDM 250 260 270 280 290 300 EFVQFHPTGI YSAGCLITEG ARGEGGYLVN ANGERFMERY APAAKDLASR DVVSRAMTIE 310 320 330 340 350 360 IREGRGVGEH KDHVFLHLNH LSPEILHRRL PGISETAKIF AGVDVTKDPI PVLPTVHYNM 370 380 390 400 410 420 GGIPTNYQGQ VIIKDGKNHN SIVNGIMAIG EAACVSVHGA NRLGSNSLLD LVVFGRSSAL 430 440 450 460 470 480 KAAELIKPAS PHKPLQKETL EKIINRFDKV RYANGNILVA DLRLKMQRTM QSHVSVFRTQ 490 500 510 520 530 540 KLLDEGVGMI SEIRNRYKDI KINDKSLIWN SDLVEALELD NLLDQALVTV CSAAARKESR 550 560 570 580 590 GAHAREDYPD RNDRDWIKHT LSSIDDSGKV VLDYKPVTLT TLTDAISAIP PVKRVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the Rickettsia prowazekii gene encoding the flavoprotein subunit of succinate dehydrogenase." Aliabadi Z., Winkler H.H., Wood D.O. Gene 133:135-140(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Madrid E.
[2]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M88696 Unassigned DNA. Translation: AAA16097.1. U02603 Unassigned DNA. Translation: AAA18327.1. AJ235270 Genomic DNA. Translation: CAA14597.1.
PIR	JN0895.
RefSeq	NP_220520.1. NC_000963.1.
3D structure databases
ProteinModelPortal	P31038.
SMR	P31038. Positions 5-596.
ModBase	Search...
Protein-protein interaction databases
STRING	272947.RP128.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA14597; CAA14597; CAA14597.
GeneID	883752.
KEGG	rpr:RP128.
PATRIC	17900996. VBIRicPro72556_0130.
Phylogenomic databases
eggNOG	COG1053.
HOGENOM	HOG000160475.
KO	K00239.
OMA	LMANAIT.
ProtClustDB	PRK09078.
Enzyme and pathway databases
UniPathway	UPA00223; UER01005.
Family and domain databases
Gene3D	1.20.58.100. 1 hit.
InterPro	IPR003953. FAD_bind_dom. IPR003952. FRD_SDH_FAD_BS. IPR015939. Fum_Rdtase/Succ_DH_flav-like_C. IPR011281. Succ_DH_flav_su_fwd. IPR014006. Succ_Dhase_FrdA_Gneg. [Graphical view]
PANTHER	PTHR11632:SF5. PTHR11632:SF5. 1 hit.
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
SUPFAM	SSF46977. Succ_DH_flav_C. 1 hit.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_RICPR

Accession

Primary (citable) accession number: P31038

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program