Reviewed, UniProtKB/Swiss-Prot P31038 (DHSA_RICPR)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase flavoprotein subunit
    EC=1.3.99.1

Gene names

Name:	sdhA
Ordered Locus Names:	RP128

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and an hydrophobic anchor protein.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Succinate dehydrogenase flavoprotein subunit		PRO_0000158657
Regions
	Nucleotide binding	41 – 56	16	FAD By similarity
Sites
	Active site	290	1	Proton acceptor By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	258	1	Substrate By similarity
	Binding site	357	1	Substrate By similarity
	Binding site	402	1	Substrate By similarity
Amino acid modifications
	Modified residue	49	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31038-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: E5CAA5DFE9426EC4	FASTA	596	65,445
	10 20 30 40 50 60 MTKAYNIIHH KFDVVVVGAG GAGLRSAFGM AKEGLNTACI TKIFPTRSHT VAAQGGISAA 70 80 90 100 110 120 LGNMGEDDWR WHMYDTVKGS DWLGDQDAIE YMCKNAPDAI LELEHYGVPF SRTVDGKIYQ 130 140 150 160 170 180 RPFGGMTTEY GKGKAAQRTC AAADRTGHAI LHTLYQQSLK HKVQFFIEYF AIDLLMEDGE 190 200 210 220 230 240 CRGVVAWNLD DGSLHCFRAH NVVLATGGYG RAYFSATSAH TCTGDGGGMV IRAGLPLQDM 250 260 270 280 290 300 EFVQFHPTGI YSAGCLITEG ARGEGGYLVN ANGERFMERY APAAKDLASR DVVSRAMTIE 310 320 330 340 350 360 IREGRGVGEH KDHVFLHLNH LSPEILHRRL PGISETAKIF AGVDVTKDPI PVLPTVHYNM 370 380 390 400 410 420 GGIPTNYQGQ VIIKDGKNHN SIVNGIMAIG EAACVSVHGA NRLGSNSLLD LVVFGRSSAL 430 440 450 460 470 480 KAAELIKPAS PHKPLQKETL EKIINRFDKV RYANGNILVA DLRLKMQRTM QSHVSVFRTQ 490 500 510 520 530 540 KLLDEGVGMI SEIRNRYKDI KINDKSLIWN SDLVEALELD NLLDQALVTV CSAAARKESR 550 560 570 580 590 GAHAREDYPD RNDRDWIKHT LSSIDDSGKV VLDYKPVTLT TLTDAISAIP PVKRVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the Rickettsia prowazekii gene encoding the flavoprotein subunit of succinate dehydrogenase." Aliabadi Z., Winkler H.H., Wood D.O. Gene 133:135-140(1993) [PubMed: 8224887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Madrid E.
[2]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M88696 Unassigned DNA. Translation: AAA16097.1. U02603 Unassigned DNA. Translation: AAA18327.1. AJ235270 Genomic DNA. Translation: CAA14597.1.
PIR	JN0895.
RefSeq	NP_220520.1.
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P10444.
ModBase	Search...
Genome annotation databases
GeneID	883752.
GenomeReviews	Gene locus RP128 in contig AJ235269_GR.
KEGG	rpr:RP128.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P31038.
OMA	P31038. TLEFDNL.
Enzyme and pathway databases
BioCyc	RPRO272947:RP128-MON.
BRENDA	1.3.99.1. 141977.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR011281. Succ_DH_flav_su_fwd. IPR014006. Succ_Dhase_frdA_Gneg. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_RICPR

Accession

Primary (citable) accession number: P31038

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)