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P31033

- MTM4_NEIGO

UniProt

P31033 - MTM4_NEIGO

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Protein

Modification methylase NgoMIV

Gene

ngoMIVM

Organism
Neisseria gonorrhoeae
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GCCGGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ngomIV endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei74 – 741PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase NgoMIV (EC:2.1.1.37)
Short name:
M.NgoMIV
Alternative name(s):
Cytosine-specific methyltransferase NgoMIV
Gene namesi
Name:ngoMIVM
OrganismiNeisseria gonorrhoeae
Taxonomic identifieri485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Modification methylase NgoMIVPRO_0000087899Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP31033.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 311309SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31033-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQFTSLEICA GAGGQALGLE RAGFSHVALI EIEPSACQTL RLNRPDWNVI
60 70 80 90 100
EGDVRLFQGE GYDGIDLLAG GVPCPPFSKA GKQLGKDDER DLFPEAIRLA
110 120 130 140 150
KETDPKAIML ENVRGLLDPK FENYRNHITE QFAKLGYLGQ WKLLYAADYG
160 170 180 190 200
VSQLRPRVLF VALKNEYTNF FKWPEPNSEQ PKTVGELLFD LMSENNWQGA
210 220 230 240 250
HNWRLKAAQI APTLVAVQKN TAVLTWDLHD PNAHGRSWVW MVQVCGIVRR
260 270 280 290 300
LKTFTGMPRL TVRMTARIQG FPDDWQFFGK KTPMYRQIGN AFPPPVAEAV
310
GRQIIKALKK EN
Length:312
Mass (Da):35,226
Last modified:July 1, 1993 - v1
Checksum:i319E20242B873452
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86915 Genomic DNA. No translation available.
PIRiA42709.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86915 Genomic DNA. No translation available.
PIRi A42709.

3D structure databases

ProteinModelPortali P31033.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Construction of a Neisseria gonorrhoeae MS11 derivative deficient in NgoMI restriction and modification."
    Stein D.C., Chien R., Seifert H.S.
    J. Bacteriol. 174:4899-4906(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MS11.

Entry informationi

Entry nameiMTM4_NEIGO
AccessioniPrimary (citable) accession number: P31033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 1, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally known as M.nGmomI.1 Publication

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3