Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P31033 (MTM4_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase NgoMIV

Short name=M.NgoMIV
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase NgoMIV
Gene names
Name:ngoMIVM
OrganismNeisseria gonorrhoeae
Taxonomic identifier485 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GCCGGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ngomIV endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Caution

Was originally (Ref.1) known as M.nGmomI.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 312312Modification methylase NgoMIV
PRO_0000087899

Regions

Domain3 – 311309SAM-dependent MTase C5-type

Sites

Active site741 By similarity

Sequences

Sequence LengthMass (Da)Tools
P31033 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 319E20242B873452

FASTA31235,226
        10         20         30         40         50         60 
MQFTSLEICA GAGGQALGLE RAGFSHVALI EIEPSACQTL RLNRPDWNVI EGDVRLFQGE 

        70         80         90        100        110        120 
GYDGIDLLAG GVPCPPFSKA GKQLGKDDER DLFPEAIRLA KETDPKAIML ENVRGLLDPK 

       130        140        150        160        170        180 
FENYRNHITE QFAKLGYLGQ WKLLYAADYG VSQLRPRVLF VALKNEYTNF FKWPEPNSEQ 

       190        200        210        220        230        240 
PKTVGELLFD LMSENNWQGA HNWRLKAAQI APTLVAVQKN TAVLTWDLHD PNAHGRSWVW 

       250        260        270        280        290        300 
MVQVCGIVRR LKTFTGMPRL TVRMTARIQG FPDDWQFFGK KTPMYRQIGN AFPPPVAEAV 

       310 
GRQIIKALKK EN 

« Hide

References

[1]"Construction of a Neisseria gonorrhoeae MS11 derivative deficient in NgoMI restriction and modification."
Stein D.C., Chien R., Seifert H.S.
J. Bacteriol. 174:4899-4906(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: MS11.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M86915 Genomic DNA. No translation available.
PIRA42709.

3D structure databases

ProteinModelPortalP31033.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 2 hits.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTM4_NEIGO
AccessionPrimary (citable) accession number: P31033
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries