P31033 (MTM4_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase NgoMIV
Short name=M.NgoMIV
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase NgoMIV

Gene names

Name:

ngoMIVM

Organism

Neisseria gonorrhoeae

Taxonomic identifier

485 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GCCGGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ngomIV endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.
Caution	Was originally (Ref.1) known as M.nGmomI.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Modification methylase NgoMIV		PRO_0000087899
Sites
	Active site	74	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31033 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 319E20242B873452	FASTA	312	35,226
	10 20 30 40 50 60 MQFTSLEICA GAGGQALGLE RAGFSHVALI EIEPSACQTL RLNRPDWNVI EGDVRLFQGE 70 80 90 100 110 120 GYDGIDLLAG GVPCPPFSKA GKQLGKDDER DLFPEAIRLA KETDPKAIML ENVRGLLDPK 130 140 150 160 170 180 FENYRNHITE QFAKLGYLGQ WKLLYAADYG VSQLRPRVLF VALKNEYTNF FKWPEPNSEQ 190 200 210 220 230 240 PKTVGELLFD LMSENNWQGA HNWRLKAAQI APTLVAVQKN TAVLTWDLHD PNAHGRSWVW 250 260 270 280 290 300 MVQVCGIVRR LKTFTGMPRL TVRMTARIQG FPDDWQFFGK KTPMYRQIGN AFPPPVAEAV 310 GRQIIKALKK EN

References

[1]	"Construction of a Neisseria gonorrhoeae MS11 derivative deficient in NgoMI restriction and modification." Stein D.C., Chien R., Seifert H.S. J. Bacteriol. 174:4899-4906(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MS11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86915 Genomic DNA. No translation available.
PIR	A42709.
3D structure databases
ProteinModelPortal	P31033.
ModBase	Search...
Protein family/group databases
REBASE	3463. M.NgoMIV.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTM4_NEIGO

Accession

Primary (citable) accession number: P31033

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program