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Protein

Type-2 restriction enzyme NgoMIV

Gene

ngoMIVR

Organism
Neisseria gonorrhoeae
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GCCGGC and cleaves after G-1.

Caution

Was originally known as R.ngomI.1 Publication

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Mg2+Note: Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi140Magnesium 11
Metal bindingi140Magnesium 21
Metal bindingi186Magnesium 1; via carbonyl oxygen1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme NgoMIV (EC:3.1.21.4)
Short name:
R.NgoMIV
Alternative name(s):
Endonuclease NgoMIV
Type II restriction enzyme NgoMIV
Gene namesi
Name:ngoMIVR
OrganismiNeisseria gonorrhoeae
Taxonomic identifieri485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773471 – 286Type-2 restriction enzyme NgoMIVAdd BLAST286

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 18Combined sources15
Helixi38 – 50Combined sources13
Helixi62 – 81Combined sources20
Helixi82 – 84Combined sources3
Turni85 – 87Combined sources3
Beta strandi92 – 96Combined sources5
Beta strandi99 – 101Combined sources3
Helixi104 – 107Combined sources4
Helixi109 – 112Combined sources4
Helixi113 – 121Combined sources9
Helixi125 – 131Combined sources7
Beta strandi140 – 145Combined sources6
Helixi150 – 153Combined sources4
Beta strandi155 – 157Combined sources3
Beta strandi179 – 189Combined sources11
Helixi194 – 196Combined sources3
Helixi197 – 208Combined sources12
Beta strandi210 – 212Combined sources3
Beta strandi215 – 220Combined sources6
Helixi225 – 232Combined sources8
Beta strandi233 – 237Combined sources5
Beta strandi239 – 243Combined sources5
Helixi246 – 256Combined sources11
Helixi259 – 270Combined sources12
Beta strandi273 – 276Combined sources4
Helixi277 – 279Combined sources3
Helixi280 – 283Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FIUX-ray1.60A/B/C/D1-286[»]
4ABTX-ray2.22A/B3-286[»]
ProteinModelPortaliP31032
SMRiP31032
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31032

Family & Domainsi

Family and domain databases

Gene3Di3.40.50.10010, 1 hit
InterProiView protein in InterPro
IPR015105 NgoMIV
IPR037083 NgoMIV_sf
IPR011335 Restrct_endonuc-II-like
PfamiView protein in Pfam
PF09015 NgoMIV_restric, 1 hit
SUPFAMiSSF52980 SSF52980, 1 hit

Sequencei

Sequence statusi: Complete.

P31032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPLFTQERR IFHKKLLDGN ILATNNRGVV SNADGSNTRS FNIAKGIADL
60 70 80 90 100
LHSETVSERL PGQTSGNAFE AICSEFVQSA FEKLQHIRPG DWNVKQVGSR
110 120 130 140 150
NRLEIARYQQ YAHLTALAKA AEENPELAAA LGSDYTITPD IIVTRNLIAD
160 170 180 190 200
AEINRNEFLV DENIATYASL RAGNGNMPLL HASISCKWTI RSDRAQNARS
210 220 230 240 250
EGLNLVRNRK GRLPHIVVVT AEPTPSRISS IALGTGEIDC VYHFALYELE
260 270 280
QILQSLNYED ALDLFYIMVN GKRLKDISDL PLDLAV
Length:286
Mass (Da):31,760
Last modified:July 1, 1993 - v1
Checksum:i6609772EF21083F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86915 Genomic DNA No translation available.
PIRiB42709
RefSeqiWP_003688519.1, NZ_NGQH01000002.1

Similar proteinsi

Entry informationi

Entry nameiT2M4_NEIGO
AccessioniPrimary (citable) accession number: P31032
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure
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Main funding by: National Institutes of Health