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P31032

- T2M4_NEIGO

UniProt

P31032 - T2M4_NEIGO

Protein

Type-2 restriction enzyme NgoMIV

Gene

ngoMIVR

Organism
Neisseria gonorrhoeae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GCCGGC and cleaves after G-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Binds 2 magnesium ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi140 – 1401Magnesium 1
    Metal bindingi140 – 1401Magnesium 2
    Metal bindingi186 – 1861Magnesium 1; via carbonyl oxygen

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme NgoMIV (EC:3.1.21.4)
    Short name:
    R.NgoMIV
    Alternative name(s):
    Endonuclease NgoMIV
    Type II restriction enzyme NgoMIV
    Gene namesi
    Name:ngoMIVR
    OrganismiNeisseria gonorrhoeae
    Taxonomic identifieri485 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 286286Type-2 restriction enzyme NgoMIVPRO_0000077347Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    Secondary structure

    1
    286
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1815
    Helixi38 – 5013
    Helixi62 – 8120
    Helixi82 – 843
    Turni85 – 873
    Beta strandi92 – 965
    Beta strandi99 – 1013
    Helixi104 – 1074
    Helixi109 – 1124
    Helixi113 – 1219
    Helixi125 – 1317
    Beta strandi140 – 1456
    Helixi150 – 1534
    Beta strandi155 – 1573
    Beta strandi179 – 18911
    Helixi194 – 1963
    Helixi197 – 20812
    Beta strandi210 – 2123
    Beta strandi215 – 2206
    Helixi225 – 2328
    Beta strandi233 – 2375
    Beta strandi239 – 2435
    Helixi246 – 25611
    Helixi259 – 27012
    Beta strandi273 – 2764
    Helixi277 – 2793
    Helixi280 – 2834

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FIUX-ray1.60A/B/C/D1-286[»]
    4ABTX-ray2.22A/B3-286[»]
    ProteinModelPortaliP31032.
    SMRiP31032. Positions 1-286.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP31032.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.50.10010. 1 hit.
    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR015105. Restrct_endonuc_II_NgoMIV.
    [Graphical view]
    PfamiPF09015. NgoMIV_restric. 1 hit.
    [Graphical view]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P31032-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPLFTQERR IFHKKLLDGN ILATNNRGVV SNADGSNTRS FNIAKGIADL    50
    LHSETVSERL PGQTSGNAFE AICSEFVQSA FEKLQHIRPG DWNVKQVGSR 100
    NRLEIARYQQ YAHLTALAKA AEENPELAAA LGSDYTITPD IIVTRNLIAD 150
    AEINRNEFLV DENIATYASL RAGNGNMPLL HASISCKWTI RSDRAQNARS 200
    EGLNLVRNRK GRLPHIVVVT AEPTPSRISS IALGTGEIDC VYHFALYELE 250
    QILQSLNYED ALDLFYIMVN GKRLKDISDL PLDLAV 286
    Length:286
    Mass (Da):31,760
    Last modified:July 1, 1993 - v1
    Checksum:i6609772EF21083F6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86915 Genomic DNA. No translation available.
    PIRiB42709.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86915 Genomic DNA. No translation available.
    PIRi B42709.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FIU X-ray 1.60 A/B/C/D 1-286 [» ]
    4ABT X-ray 2.22 A/B 3-286 [» ]
    ProteinModelPortali P31032.
    SMRi P31032. Positions 1-286.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P31032.

    Family and domain databases

    Gene3Di 3.40.50.10010. 1 hit.
    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR015105. Restrct_endonuc_II_NgoMIV.
    [Graphical view ]
    Pfami PF09015. NgoMIV_restric. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Construction of a Neisseria gonorrhoeae MS11 derivative deficient in NgoMI restriction and modification."
      Stein D.C., Chien R., Seifert H.S.
      J. Bacteriol. 174:4899-4906(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: MS11.
    2. "Structure of the tetrameric restriction endonuclease NgoMIV in complex with cleaved DNA."
      Deibert M., Grazulis S., Sasnauskas G., Siksnys V., Huber R.
      Nat. Struct. Biol. 7:792-799(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

    Entry informationi

    Entry nameiT2M4_NEIGO
    AccessioniPrimary (citable) accession number: P31032
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally known as R.ngomI.1 Publication

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3