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Protein

Type-2 restriction enzyme NgoMIV

Gene

ngoMIVR

Organism
Neisseria gonorrhoeae
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GCCGGC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Mg2+Note: Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi140Magnesium 11
Metal bindingi140Magnesium 21
Metal bindingi186Magnesium 1; via carbonyl oxygen1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme NgoMIV (EC:3.1.21.4)
Short name:
R.NgoMIV
Alternative name(s):
Endonuclease NgoMIV
Type II restriction enzyme NgoMIV
Gene namesi
Name:ngoMIVR
OrganismiNeisseria gonorrhoeae
Taxonomic identifieri485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773471 – 286Type-2 restriction enzyme NgoMIVAdd BLAST286

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi528357.NgonPI_010100008387.

Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 18Combined sources15
Helixi38 – 50Combined sources13
Helixi62 – 81Combined sources20
Helixi82 – 84Combined sources3
Turni85 – 87Combined sources3
Beta strandi92 – 96Combined sources5
Beta strandi99 – 101Combined sources3
Helixi104 – 107Combined sources4
Helixi109 – 112Combined sources4
Helixi113 – 121Combined sources9
Helixi125 – 131Combined sources7
Beta strandi140 – 145Combined sources6
Helixi150 – 153Combined sources4
Beta strandi155 – 157Combined sources3
Beta strandi179 – 189Combined sources11
Helixi194 – 196Combined sources3
Helixi197 – 208Combined sources12
Beta strandi210 – 212Combined sources3
Beta strandi215 – 220Combined sources6
Helixi225 – 232Combined sources8
Beta strandi233 – 237Combined sources5
Beta strandi239 – 243Combined sources5
Helixi246 – 256Combined sources11
Helixi259 – 270Combined sources12
Beta strandi273 – 276Combined sources4
Helixi277 – 279Combined sources3
Helixi280 – 283Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FIUX-ray1.60A/B/C/D1-286[»]
4ABTX-ray2.22A/B3-286[»]
ProteinModelPortaliP31032.
SMRiP31032.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31032.

Family & Domainsi

Family and domain databases

Gene3Di3.40.50.10010. 1 hit.
InterProiIPR015105. NgoMIV.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF09015. NgoMIV_restric. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P31032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPLFTQERR IFHKKLLDGN ILATNNRGVV SNADGSNTRS FNIAKGIADL
60 70 80 90 100
LHSETVSERL PGQTSGNAFE AICSEFVQSA FEKLQHIRPG DWNVKQVGSR
110 120 130 140 150
NRLEIARYQQ YAHLTALAKA AEENPELAAA LGSDYTITPD IIVTRNLIAD
160 170 180 190 200
AEINRNEFLV DENIATYASL RAGNGNMPLL HASISCKWTI RSDRAQNARS
210 220 230 240 250
EGLNLVRNRK GRLPHIVVVT AEPTPSRISS IALGTGEIDC VYHFALYELE
260 270 280
QILQSLNYED ALDLFYIMVN GKRLKDISDL PLDLAV
Length:286
Mass (Da):31,760
Last modified:July 1, 1993 - v1
Checksum:i6609772EF21083F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86915 Genomic DNA. No translation available.
PIRiB42709.
RefSeqiWP_003688519.1. NZ_LOIB01000107.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86915 Genomic DNA. No translation available.
PIRiB42709.
RefSeqiWP_003688519.1. NZ_LOIB01000107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FIUX-ray1.60A/B/C/D1-286[»]
4ABTX-ray2.22A/B3-286[»]
ProteinModelPortaliP31032.
SMRiP31032.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi528357.NgonPI_010100008387.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP31032.

Family and domain databases

Gene3Di3.40.50.10010. 1 hit.
InterProiIPR015105. NgoMIV.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF09015. NgoMIV_restric. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiT2M4_NEIGO
AccessioniPrimary (citable) accession number: P31032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally known as R.ngomI.1 Publication

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.