Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P31032 (T2M4_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme NgoMIV

Short name=R.NgoMIV
EC=3.1.21.4
Alternative name(s):
Endonuclease NgoMIV
Type II restriction enzyme NgoMIV
Gene names
Name:ngoMIVR
OrganismNeisseria gonorrhoeae
Taxonomic identifier485 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence GCCGGC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Binds 2 magnesium ions per subunit.

Subunit structure

Homotetramer.

Caution

Was originally (Ref.1) known as R.ngomI.

Ontologies

Keywords
   Biological processRestriction system
   LigandMagnesium
Metal-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 286286Type-2 restriction enzyme NgoMIV
PRO_0000077347

Sites

Metal binding1401Magnesium 1
Metal binding1401Magnesium 2
Metal binding1861Magnesium 1; via carbonyl oxygen

Secondary structure

................................................ 286
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P31032 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 6609772EF21083F6

FASTA28631,760
        10         20         30         40         50         60 
MNPLFTQERR IFHKKLLDGN ILATNNRGVV SNADGSNTRS FNIAKGIADL LHSETVSERL 

        70         80         90        100        110        120 
PGQTSGNAFE AICSEFVQSA FEKLQHIRPG DWNVKQVGSR NRLEIARYQQ YAHLTALAKA 

       130        140        150        160        170        180 
AEENPELAAA LGSDYTITPD IIVTRNLIAD AEINRNEFLV DENIATYASL RAGNGNMPLL 

       190        200        210        220        230        240 
HASISCKWTI RSDRAQNARS EGLNLVRNRK GRLPHIVVVT AEPTPSRISS IALGTGEIDC 

       250        260        270        280 
VYHFALYELE QILQSLNYED ALDLFYIMVN GKRLKDISDL PLDLAV 

« Hide

References

[1]"Construction of a Neisseria gonorrhoeae MS11 derivative deficient in NgoMI restriction and modification."
Stein D.C., Chien R., Seifert H.S.
J. Bacteriol. 174:4899-4906(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: MS11.
[2]"Structure of the tetrameric restriction endonuclease NgoMIV in complex with cleaved DNA."
Deibert M., Grazulis S., Sasnauskas G., Siksnys V., Huber R.
Nat. Struct. Biol. 7:792-799(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M86915 Genomic DNA. No translation available.
PIRB42709.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FIUX-ray1.60A/B/C/D3-286[»]
4ABTX-ray2.22A/B3-286[»]
ProteinModelPortalP31032.
SMRP31032. Positions 1-286.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.10010. 1 hit.
InterProIPR011335. Restrct_endonuc-II-like.
IPR015105. Restrct_endonuc_II_NgoMIV.
[Graphical view]
PfamPF09015. NgoMIV_restric. 1 hit.
[Graphical view]
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP31032.

Entry information

Entry nameT2M4_NEIGO
AccessionPrimary (citable) accession number: P31032
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references