Reviewed, UniProtKB/Swiss-Prot P31032 (T2M4_NEIGO)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme NgoMIV
      Short name=R.NgoMIV
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme NgoMIV
    Endonuclease NgoMIV

Gene names

Name:

ngoMIVR

Organism

Neisseria gonorrhoeae

Taxonomic identifier

485 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GCCGGC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Binds 2 magnesium ions per subunit.
Subunit structure	Homotetramer.
Caution	Was originally (Ref.1) known as R.ngomI.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 286

286

Type-2 restriction enzyme NgoMIV

PRO_0000077347

Sites

Metal binding

140

1

Magnesium 1

Metal binding

140

1

Magnesium 2

Metal binding

186

1

Magnesium 1; via carbonyl oxygen

Secondary structure

1

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286

Helix Strand Turn

Details...

Helix

4 – 18

15

Helix

38 – 50

13

Helix

62 – 81

20

Helix

82 – 84

3

Turn

85 – 87

3

Beta strand

92 – 96

5

Helix

104 – 107

4

Helix

109 – 112

4

Helix

113 – 121

9

Helix

125 – 131

7

Beta strand

140 – 145

6

Helix

150 – 153

4

Beta strand

155 – 157

3

Beta strand

179 – 189

11

Helix

194 – 196

3

Helix

197 – 208

12

Beta strand

210 – 212

3

Beta strand

215 – 220

6

Helix

225 – 232

8

Beta strand

233 – 237

5

Beta strand

239 – 243

5

Helix

246 – 256

11

Helix

259 – 270

12

Beta strand

273 – 276

4

Helix

277 – 279

3

Helix

280 – 283

4

Sequences

	Sequence		Length	Mass (Da)
	P31032-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 6609772EF21083F6	FASTA	286	31,760
	10 20 30 40 50 60 MNPLFTQERR IFHKKLLDGN ILATNNRGVV SNADGSNTRS FNIAKGIADL LHSETVSERL 70 80 90 100 110 120 PGQTSGNAFE AICSEFVQSA FEKLQHIRPG DWNVKQVGSR NRLEIARYQQ YAHLTALAKA 130 140 150 160 170 180 AEENPELAAA LGSDYTITPD IIVTRNLIAD AEINRNEFLV DENIATYASL RAGNGNMPLL 190 200 210 220 230 240 HASISCKWTI RSDRAQNARS EGLNLVRNRK GRLPHIVVVT AEPTPSRISS IALGTGEIDC 250 260 270 280 VYHFALYELE QILQSLNYED ALDLFYIMVN GKRLKDISDL PLDLAV

References

[1]	"Construction of a Neisseria gonorrhoeae MS11 derivative deficient in NgoMI restriction and modification." Stein D.C., Chien R., Seifert H.S. J. Bacteriol. 174:4899-4906(1992) [PubMed: 1321116] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MS11.
[2]	"Structure of the tetrameric restriction endonuclease NgoMIV in complex with cleaved DNA." Deibert M., Grazulis S., Sasnauskas G., Siksnys V., Huber R. Nat. Struct. Biol. 7:792-799(2000) [PubMed: 10966652] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

M86915 Genomic DNA. No translation available.

PIR

B42709.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FIU	X-ray	1.60	A/B/C/D	3-286	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.1.21.4. 588.

Family and domain databases

InterPro

IPR015105. Restrict_endonuc_II_NgoMIV.
[Graphical view]

Gene3D

G3DSA:3.40.50.10010. Restrict_endonuc_II_NgoMIV. 1 hit.

Pfam

PF09015. NgoMIV_restric. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

T2M4_NEIGO

Accession

Primary (citable) accession number: P31032

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	July 7, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)