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P31031 (T2P1_PROHU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme PvuI

Short name=R.PvuI
EC=3.1.21.4
Alternative name(s):
Endonuclease PvuI
Type II restriction enzyme PvuI
Gene names
Name:pvuIR
OrganismProteus hauseri
Taxonomic identifier183417 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus

Protein attributes

Sequence length11 AA.
Sequence statusFragment.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CGATCG and cleaves after T-4.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 11›11Type-2 restriction enzyme PvuI
PRO_0000077355

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P31031 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 9F0CDE7955B72B1A

FASTA111,300
        10 
ISVDELSNYN F 

« Hide

References

[1]"Cloning and characterization of genes for the PvuI restriction and modification system."
Smith M.D., Longo M., Gerard G.F., Chatterjee D.K.
Nucleic Acids Res. 20:5743-5747(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13315 / DSM 30118 / JCM 1668 / NBRC 3851 / NCIMB 4175 / NCTC 4175 / NRRL B-3405.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L04163 Genomic DNA. Translation: AAA25660.1.
PIRS35490.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1541. PvuI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2P1_PROHU
AccessionPrimary (citable) accession number: P31031
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries