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Reviewed, UniProtKB/Swiss-Prot P31031 (T2P1_PROVU)

Last modified July 7, 2009. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Type-2 restriction enzyme PvuI
      Short name=R.PvuI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme PvuI
    Endonuclease PvuI
Gene names
Name: pvuIR
OrganismProteus vulgaris
Taxonomic identifier585 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus

Protein attributes

Sequence length11 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is not processed.
Protein existencePredicted.

General annotation (Comments)

Function

Recognizes the double-stranded sequence CGATCG and cleaves after T-4.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 11›11Type-2 restriction enzyme PvuI
PRO_0000077355

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P31031-1 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 9F0CDE7955B72B1A

FASTA111,300
        10 
ISVDELSNYN F 

« Hide

References

[1]"Cloning and characterization of genes for the PvuI restriction and modification system."
Smith M.D., Longo M., Gerard G.F., Chatterjee D.K.
Nucleic Acids Res. 20:5743-5747(1992) [PubMed: 1454536] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13315 / DSM 30118 / IFO 3851 / NCIB 4175 / NCTC 4175.

Cross-references

Sequence databases

L04163 Genomic DNA. Translation: AAA25660.1.
PIRS35490.

3D structure databases

ModBaseSearch...

Enzyme and pathway databases

BRENDA3.1.21.4. 641.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2P1_PROVU
AccessionPrimary (citable) accession number: P31031
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 7, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents