P31030 (SPYA_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine--pyruvate aminotransferase
Short name=SPT
EC=2.6.1.51

Alternative name(s):
Alanine--glyoxylate aminotransferase
Short name=AGT
EC=2.6.1.44

Gene names

Name:	AGXT
Synonyms:	AGT1

Organism

Oryctolagus cuniculus (Rabbit) [Complete proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-serine + pyruvate = 3-hydroxypyruvate + L-alanine. L-alanine + glyoxylate = pyruvate + glycine.
Cofactor	Pyridoxal phosphate.
Subcellular location	Peroxisome.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Peroxisome
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alanine-glyoxylate transaminase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro serine-pyruvate transaminase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Serine--pyruvate aminotransferase		PRO_0000150238
Regions
	Motif	390 – 392	3	Microbody targeting signal
Sites
	Binding site	360	1	Substrate By similarity
Amino acid modifications
	Modified residue	209	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31030 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 5261DD14564582D3	FASTA	392	43,148
	10 20 30 40 50 60 MASRQLLVAP PEALRKPLCT PHRLLLGPGP SNLPPRVLAA GGLQMIGHMH EEMYQVMDEI 70 80 90 100 110 120 KQGIQYAFQT RNALTLAVSG SGHCALETAL FNLLEPGDAF LVGANGIWGQ RAAEVGERIG 130 140 150 160 170 180 ARVHPMIKDP GSHYTLQEVE ECLAQHKPVL LFLTHGESST GVLQPLDGFG ELCHRYKCLL 190 200 210 220 230 240 LVDSVASLGG APIYMDQQGI DVLYSGSQKA LNAPPGTSLI SFSDKAKSKI YARKTKPFSF 250 260 270 280 290 300 YMDVQLLANI WGCDGKPRMY HHTTPVIGIF ALRESLALLV EQGLEKSWQR HREVAQHLYR 310 320 330 340 350 360 RLQELGLQLF VKDPALRLPT VTTVIVPASY RWRDIVSYVM HHFGIEITGG LGPSADKVLR 370 380 390 IGLLGCNATR ENVDRLATAL REALQHCAQS QL

References

[1]

"Molecular evolution of alanine/glyoxylate aminotransferase 1 intracellular targeting. Analysis of the marmoset and rabbit genes." Purdue P.E., Lumb M.J., Danpure C.J. Eur. J. Biochem. 207:757-766(1992) [PubMed: 1339350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

[2]

"Mammalian alanine/glyoxylate aminotransferase 1 is imported into peroxisomes via the PTS1 translocation pathway. Increased degeneracy and context specificity of the mammalian PTS1 motif and implications for the peroxisome-to-mitochondrion mistargeting of AGT in primary hyperoxaluria type 1." Motley A., Lumb M.J., Oatey P.B., Jennings P.R., de Zoysa P.A., Wanders R.J.A., Tabak H.F., Danpure C.J. J. Cell Biol. 131:95-109(1995) [PubMed: 7559790] [Abstract] Cited for: MICROBODY TARGETING SIGNAL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84647 mRNA. Translation: AAA31158.1.
PIR	S24155.
RefSeq	NP_001075778.1. NM_001082309.1.
UniGene	Ocu.1825.
3D structure databases
ProteinModelPortal	P31030.
SMR	P31030. Positions 5-389.
ModBase	Search...
Protein-protein interaction databases
STRING	P31030.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009147.
Organism-specific databases
CTD	189.
Phylogenomic databases
GeneTree	ENSGT00390000006648.
HOVERGEN	HBG006907.
OrthoDB	EOG4BVRTQ.
Family and domain databases
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR024169. SP_NH2Trfase/AEP_transaminase. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
PIRSF	PIRSF000524. SPT. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPYA_RABIT

Accession

Primary (citable) accession number: P31030

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	November 16, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program