Reviewed, UniProtKB/Swiss-Prot P31030 (SPYA_RABIT)

Names and origin

Protein names

Recommended name:
    Serine--pyruvate aminotransferase
      Short name=SPT
    EC=2.6.1.51
Alternative name(s):
    Alanine--glyoxylate aminotransferase
      Short name=AGT
    EC=2.6.1.44

Gene names

Name:	AGXT
Synonyms:	AGT1

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-serine + pyruvate = 3-hydroxypyruvate + L-alanine. L-alanine + glyoxylate = pyruvate + glycine.
Cofactor	Pyridoxal phosphate.
Subcellular location	Peroxisome.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Peroxisome
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alanine-glyoxylate transaminase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro serine-pyruvate transaminase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Serine--pyruvate aminotransferase		PRO_0000150238
Regions
	Motif	390 – 392	3	Microbody targeting signal
Sites
	Binding site	360	1	Substrate By similarity
Amino acid modifications
	Modified residue	209	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31030-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 5261DD14564582D3	FASTA	392	43,148
	10 20 30 40 50 60 MASRQLLVAP PEALRKPLCT PHRLLLGPGP SNLPPRVLAA GGLQMIGHMH EEMYQVMDEI 70 80 90 100 110 120 KQGIQYAFQT RNALTLAVSG SGHCALETAL FNLLEPGDAF LVGANGIWGQ RAAEVGERIG 130 140 150 160 170 180 ARVHPMIKDP GSHYTLQEVE ECLAQHKPVL LFLTHGESST GVLQPLDGFG ELCHRYKCLL 190 200 210 220 230 240 LVDSVASLGG APIYMDQQGI DVLYSGSQKA LNAPPGTSLI SFSDKAKSKI YARKTKPFSF 250 260 270 280 290 300 YMDVQLLANI WGCDGKPRMY HHTTPVIGIF ALRESLALLV EQGLEKSWQR HREVAQHLYR 310 320 330 340 350 360 RLQELGLQLF VKDPALRLPT VTTVIVPASY RWRDIVSYVM HHFGIEITGG LGPSADKVLR 370 380 390 IGLLGCNATR ENVDRLATAL REALQHCAQS QL

References

[1]

"Molecular evolution of alanine/glyoxylate aminotransferase 1 intracellular targeting. Analysis of the marmoset and rabbit genes." Purdue P.E., Lumb M.J., Danpure C.J. Eur. J. Biochem. 207:757-766(1992) [PubMed: 1339350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

[2]

"Mammalian alanine/glyoxylate aminotransferase 1 is imported into peroxisomes via the PTS1 translocation pathway. Increased degeneracy and context specificity of the mammalian PTS1 motif and implications for the peroxisome-to-mitochondrion mistargeting of AGT in primary hyperoxaluria type 1." Motley A., Lumb M.J., Oatey P.B., Jennings P.R., de Zoysa P.A., Wanders R.J.A., Tabak H.F., Danpure C.J. J. Cell Biol. 131:95-109(1995) [PubMed: 7559790] [Abstract] Cited for: MICROBODY TARGETING SIGNAL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84647 mRNA. Translation: AAA31158.1.
PIR	S24155.
RefSeq	NP_001075778.1.
UniGene	Ocu.1825
3D structure databases
SMR	P31030. Positions 5-389.
ModBase	Search...
Genome annotation databases
GeneID	100009147.
Phylogenomic databases
HOVERGEN	P31030.
Enzyme and pathway databases
BRENDA	2.6.1.44. 255. 2.6.1.51. 255.
Family and domain databases
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
PROSITE	PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPYA_RABIT

Accession

Primary (citable) accession number: P31030

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	January 19, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)