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P31026

- DHE4_CORGL

UniProt

P31026 - DHE4_CORGL

Protein

NADP-specific glutamate dehydrogenase

Gene

gdh

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (26 Jul 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.By similarity

    Catalytic activityi

    L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei92 – 921SubstrateBy similarity
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei116 – 1161SubstrateBy similarity
    Active sitei128 – 1281Proton donorPROSITE-ProRule annotation
    Binding sitei167 – 1671Substrate; via carbonyl oxygenBy similarity
    Sitei168 – 1681Important for catalysisBy similarity
    Binding sitei212 – 2121NADPBy similarity
    Binding sitei243 – 2431NADPBy similarity
    Binding sitei379 – 3791SubstrateBy similarity

    GO - Molecular functioni

    1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB

    GO - Biological processi

    1. glutamate biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
    Short name:
    NADP-GDH
    Gene namesi
    Name:gdh
    Ordered Locus Names:Cgl2079, cg2280
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 447447NADP-specific glutamate dehydrogenasePRO_0000182768Add
    BLAST

    Proteomic databases

    PRIDEiP31026.

    Interactioni

    Subunit structurei

    Homohexamer.By similarity

    Protein-protein interaction databases

    STRINGi196627.cg2280.

    Structurei

    3D structure databases

    ProteinModelPortaliP31026.
    SMRiP31026. Positions 3-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0334.
    HOGENOMiHOG000243799.
    KOiK00262.
    OMAiRNTNECT.
    OrthoDBiEOG65XN4D.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31026-1 [UniParc]FASTAAdd to Basket

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    MTVDEQVSNY YDMLLKRNAG EPEFHQAVAE VLESLKIVLE KDPHYADYGL    50
    IQRLCEPERQ LIFRVPWVDD QGQVHVNRGF RVQFNSALGP YKGGLRFHPS 100
    VNLGIVKFLG FEQIFKNSLT GLPIGGGKGG SDFDPKGKSD LEIMRFCQSF 150
    MTELHRHIGE YRDVPAGDIG VGGREIGYLF GHYRRMANQH ESGVLTGKGL 200
    TWGGSLVRTE ATGYGCVYFV SEMIKAKGES ISGQKIIVSG SGNVATYAIE 250
    KAQELGATVI GFSDSSGWVH TPNGVDVAKL REIKEVRRAR VSVYADEVEG 300
    ATYHTDGSIW DLKCDIALPC ATQNELNGEN AKTLADNGCR FVAEGANMPS 350
    TPEAVEVFRE RDIRFGPGKA ANAGGVATSA LEMQQNASRD SWSFEYTDER 400
    LQVIMKNIFK TCAETAAEYG HENDYVVGAN IAGFKKVADA MLAQGVI 447
    Length:447
    Mass (Da):48,988
    Last modified:July 26, 2002 - v2
    Checksum:i0338926EA7080E35
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti37 – 371I → L in CAA42048. (PubMed:1552846)Curated
    Sequence conflicti371 – 41040ANAGG…KNIFK → TPEAVEVFRERDIRFGPGKA VNVGGVATSALEMQQNASRE in CAA42048. (PubMed:1552846)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59404 Genomic DNA. Translation: CAA42048.2.
    X72855 Genomic DNA. Translation: CAA51376.1.
    BA000036 Genomic DNA. Translation: BAB99472.1.
    BX927154 Genomic DNA. Translation: CAF20415.1.
    PIRiS32227.
    RefSeqiNP_601279.1. NC_003450.3.
    YP_226316.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB99472; BAB99472; BAB99472.
    CAF20415; CAF20415; cg2280.
    GeneIDi1020031.
    KEGGicgb:cg2280.
    cgl:NCgl1999.
    PATRICi21496158. VBICorGlu203724_2015.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59404 Genomic DNA. Translation: CAA42048.2 .
    X72855 Genomic DNA. Translation: CAA51376.1 .
    BA000036 Genomic DNA. Translation: BAB99472.1 .
    BX927154 Genomic DNA. Translation: CAF20415.1 .
    PIRi S32227.
    RefSeqi NP_601279.1. NC_003450.3.
    YP_226316.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali P31026.
    SMRi P31026. Positions 3-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg2280.

    Proteomic databases

    PRIDEi P31026.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB99472 ; BAB99472 ; BAB99472 .
    CAF20415 ; CAF20415 ; cg2280 .
    GeneIDi 1020031.
    KEGGi cgb:cg2280.
    cgl:NCgl1999.
    PATRICi 21496158. VBICorGlu203724_2015.

    Phylogenomic databases

    eggNOGi COG0334.
    HOGENOMi HOG000243799.
    KOi K00262.
    OMAi RNTNECT.
    OrthoDBi EOG65XN4D.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000185. Glu_DH. 1 hit.
    PRINTSi PR00082. GLFDHDRGNASE.
    SMARTi SM00839. ELFV_dehydrog. 1 hit.
    [Graphical view ]
    PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase."
      Boermann E.R., Eikmanns B.J., Sahm H.
      Mol. Microbiol. 6:317-326(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    2. Guyonvarch A., David F., Leblon G.
      Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiDHE4_CORGL
    AccessioniPrimary (citable) accession number: P31026
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 26, 2002
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3