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P31026

- DHE4_CORGL

UniProt

P31026 - DHE4_CORGL

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Protein
NADP-specific glutamate dehydrogenase
Gene
gdh, Cgl2079, cg2280
Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia By similarity.

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921Substrate By similarity
Binding sitei113 – 1131Substrate By similarity
Binding sitei116 – 1161Substrate By similarity
Active sitei128 – 1281Proton donor By similarity
Binding sitei167 – 1671Substrate; via carbonyl oxygen By similarity
Sitei168 – 1681Important for catalysis By similarity
Binding sitei212 – 2121NADP By similarity
Binding sitei243 – 2431NADP By similarity
Binding sitei379 – 3791Substrate By similarity

GO - Molecular functioni

  1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB

GO - Biological processi

  1. glutamate biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
Gene namesi
Name:gdh
Ordered Locus Names:Cgl2079, cg2280
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447NADP-specific glutamate dehydrogenase
PRO_0000182768Add
BLAST

Proteomic databases

PRIDEiP31026.

Interactioni

Subunit structurei

Homohexamer By similarity.

Protein-protein interaction databases

STRINGi196627.cg2280.

Structurei

3D structure databases

ProteinModelPortaliP31026.
SMRiP31026. Positions 3-446.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0334.
HOGENOMiHOG000243799.
KOiK00262.
OMAiRNTNECT.
OrthoDBiEOG65XN4D.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31026-1 [UniParc]FASTAAdd to Basket

« Hide

MTVDEQVSNY YDMLLKRNAG EPEFHQAVAE VLESLKIVLE KDPHYADYGL    50
IQRLCEPERQ LIFRVPWVDD QGQVHVNRGF RVQFNSALGP YKGGLRFHPS 100
VNLGIVKFLG FEQIFKNSLT GLPIGGGKGG SDFDPKGKSD LEIMRFCQSF 150
MTELHRHIGE YRDVPAGDIG VGGREIGYLF GHYRRMANQH ESGVLTGKGL 200
TWGGSLVRTE ATGYGCVYFV SEMIKAKGES ISGQKIIVSG SGNVATYAIE 250
KAQELGATVI GFSDSSGWVH TPNGVDVAKL REIKEVRRAR VSVYADEVEG 300
ATYHTDGSIW DLKCDIALPC ATQNELNGEN AKTLADNGCR FVAEGANMPS 350
TPEAVEVFRE RDIRFGPGKA ANAGGVATSA LEMQQNASRD SWSFEYTDER 400
LQVIMKNIFK TCAETAAEYG HENDYVVGAN IAGFKKVADA MLAQGVI 447
Length:447
Mass (Da):48,988
Last modified:July 26, 2002 - v2
Checksum:i0338926EA7080E35
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371I → L in CAA42048. 1 Publication
Sequence conflicti371 – 41040ANAGG…KNIFK → TPEAVEVFRERDIRFGPGKA VNVGGVATSALEMQQNASRE in CAA42048. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59404 Genomic DNA. Translation: CAA42048.2.
X72855 Genomic DNA. Translation: CAA51376.1.
BA000036 Genomic DNA. Translation: BAB99472.1.
BX927154 Genomic DNA. Translation: CAF20415.1.
PIRiS32227.
RefSeqiNP_601279.1. NC_003450.3.
YP_226316.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99472; BAB99472; BAB99472.
CAF20415; CAF20415; cg2280.
GeneIDi1020031.
KEGGicgb:cg2280.
cgl:NCgl1999.
PATRICi21496158. VBICorGlu203724_2015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59404 Genomic DNA. Translation: CAA42048.2 .
X72855 Genomic DNA. Translation: CAA51376.1 .
BA000036 Genomic DNA. Translation: BAB99472.1 .
BX927154 Genomic DNA. Translation: CAF20415.1 .
PIRi S32227.
RefSeqi NP_601279.1. NC_003450.3.
YP_226316.1. NC_006958.1.

3D structure databases

ProteinModelPortali P31026.
SMRi P31026. Positions 3-446.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg2280.

Proteomic databases

PRIDEi P31026.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB99472 ; BAB99472 ; BAB99472 .
CAF20415 ; CAF20415 ; cg2280 .
GeneIDi 1020031.
KEGGi cgb:cg2280.
cgl:NCgl1999.
PATRICi 21496158. VBICorGlu203724_2015.

Phylogenomic databases

eggNOGi COG0334.
HOGENOMi HOG000243799.
KOi K00262.
OMAi RNTNECT.
OrthoDBi EOG65XN4D.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000185. Glu_DH. 1 hit.
PRINTSi PR00082. GLFDHDRGNASE.
SMARTi SM00839. ELFV_dehydrog. 1 hit.
[Graphical view ]
PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase."
    Boermann E.R., Eikmanns B.J., Sahm H.
    Mol. Microbiol. 6:317-326(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Guyonvarch A., David F., Leblon G.
    Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiDHE4_CORGL
AccessioniPrimary (citable) accession number: P31026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 26, 2002
Last modified: July 9, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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