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P31026 (DHE4_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADP-specific glutamate dehydrogenase

Short name=NADP-GDH
EC=1.4.1.4
Gene names
Name:gdh
Ordered Locus Names:Cgl2079, cg2280
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length447 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia By similarity.

Catalytic activity

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Subunit structure

Homohexamer By similarity.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamate biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionglutamate dehydrogenase (NADP+) activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 447447NADP-specific glutamate dehydrogenase
PRO_0000182768

Sites

Active site1281Proton donor By similarity
Binding site921Substrate By similarity
Binding site1131Substrate By similarity
Binding site1161Substrate By similarity
Binding site1671Substrate; via carbonyl oxygen By similarity
Binding site2121NADP By similarity
Binding site2431NADP By similarity
Binding site3791Substrate By similarity
Site1681Important for catalysis By similarity

Experimental info

Sequence conflict371I → L in CAA42048. Ref.1
Sequence conflict371 – 41040ANAGG…KNIFK → TPEAVEVFRERDIRFGPGKA VNVGGVATSALEMQQNASRE in CAA42048. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P31026 [UniParc].

Last modified July 26, 2002. Version 2.
Checksum: 0338926EA7080E35

FASTA44748,988
        10         20         30         40         50         60 
MTVDEQVSNY YDMLLKRNAG EPEFHQAVAE VLESLKIVLE KDPHYADYGL IQRLCEPERQ 

        70         80         90        100        110        120 
LIFRVPWVDD QGQVHVNRGF RVQFNSALGP YKGGLRFHPS VNLGIVKFLG FEQIFKNSLT 

       130        140        150        160        170        180 
GLPIGGGKGG SDFDPKGKSD LEIMRFCQSF MTELHRHIGE YRDVPAGDIG VGGREIGYLF 

       190        200        210        220        230        240 
GHYRRMANQH ESGVLTGKGL TWGGSLVRTE ATGYGCVYFV SEMIKAKGES ISGQKIIVSG 

       250        260        270        280        290        300 
SGNVATYAIE KAQELGATVI GFSDSSGWVH TPNGVDVAKL REIKEVRRAR VSVYADEVEG 

       310        320        330        340        350        360 
ATYHTDGSIW DLKCDIALPC ATQNELNGEN AKTLADNGCR FVAEGANMPS TPEAVEVFRE 

       370        380        390        400        410        420 
RDIRFGPGKA ANAGGVATSA LEMQQNASRD SWSFEYTDER LQVIMKNIFK TCAETAAEYG 

       430        440 
HENDYVVGAN IAGFKKVADA MLAQGVI 

« Hide

References

« Hide 'large scale' references
[1]"Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase."
Boermann E.R., Eikmanns B.J., Sahm H.
Mol. Microbiol. 6:317-326(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]Guyonvarch A., David F., Leblon G.
Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59404 Genomic DNA. Translation: CAA42048.2.
X72855 Genomic DNA. Translation: CAA51376.1.
BA000036 Genomic DNA. Translation: BAB99472.1.
BX927154 Genomic DNA. Translation: CAF20415.1.
PIRS32227.
RefSeqNP_601279.1. NC_003450.3.
YP_226316.1. NC_006958.1.

3D structure databases

ProteinModelPortalP31026.
SMRP31026. Positions 3-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg2280.

Proteomic databases

PRIDEP31026.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB99472; BAB99472; BAB99472.
CAF20415; CAF20415; cg2280.
GeneID1020031.
KEGGcgb:cg2280.
cgl:NCgl1999.
PATRIC21496158. VBICorGlu203724_2015.

Phylogenomic databases

eggNOGCOG0334.
HOGENOMHOG000243799.
KOK00262.
OMARNTNECT.
OrthoDBEOG65XN4D.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHE4_CORGL
AccessionPrimary (citable) accession number: P31026
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 26, 2002
Last modified: July 9, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families