Reviewed, UniProtKB/Swiss-Prot P31026 (DHE4_CORGL)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4

Gene names

Name:	gdh
Ordered Locus Names:	Cgl2079, cg2280

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	NADP-specific glutamate dehydrogenase		PRO_0000182768
Sites
	Active site	128	1	By similarity
Experimental info
	Sequence conflict	37	1	I → L in CAA42048. Ref.1
	Sequence conflict	371 – 410	40	ANAGG…KNIFK → TPEAVEVFRERDIRFGPGKA VNVGGVATSALEMQQNASRE Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P31026-1 [UniParc]. Last modified July 26, 2002. Version 2. Checksum: 0338926EA7080E35	FASTA	447	48,988
	10 20 30 40 50 60 MTVDEQVSNY YDMLLKRNAG EPEFHQAVAE VLESLKIVLE KDPHYADYGL IQRLCEPERQ 70 80 90 100 110 120 LIFRVPWVDD QGQVHVNRGF RVQFNSALGP YKGGLRFHPS VNLGIVKFLG FEQIFKNSLT 130 140 150 160 170 180 GLPIGGGKGG SDFDPKGKSD LEIMRFCQSF MTELHRHIGE YRDVPAGDIG VGGREIGYLF 190 200 210 220 230 240 GHYRRMANQH ESGVLTGKGL TWGGSLVRTE ATGYGCVYFV SEMIKAKGES ISGQKIIVSG 250 260 270 280 290 300 SGNVATYAIE KAQELGATVI GFSDSSGWVH TPNGVDVAKL REIKEVRRAR VSVYADEVEG 310 320 330 340 350 360 ATYHTDGSIW DLKCDIALPC ATQNELNGEN AKTLADNGCR FVAEGANMPS TPEAVEVFRE 370 380 390 400 410 420 RDIRFGPGKA ANAGGVATSA LEMQQNASRD SWSFEYTDER LQVIMKNIFK TCAETAAEYG 430 440 HENDYVVGAN IAGFKKVADA MLAQGVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase." Boermann E.R., Eikmanns B.J., Sahm H. Mol. Microbiol. 6:317-326(1992) [PubMed: 1552846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	Guyonvarch A., David F., Leblon G. Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59404 Genomic DNA. Translation: CAA42048.2. X72855 Genomic DNA. Translation: CAA51376.1. BA000036 Genomic DNA. Translation: BAB99472.1. BX927154 Genomic DNA. Translation: CAF20415.1.
PIR	S32227.
RefSeq	NP_601279.1. YP_226316.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Genome annotation databases
GeneID	1020031. 3343980.
GenomeReviews	Gene locus Cgl2079 in contig BA000036_GR. Gene locus cg2280 in contig BX927147_GR.
KEGG	cgb:cg2280. cgl:NCgl1999.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P31026.
OMA	P31026. EMAQNAS.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG2280-MON.
BRENDA	1.4.1.4. 812.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_CORGL

Accession

Primary (citable) accession number: P31026

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 26, 2002
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)