P31025 (LCN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipocalin-1

Alternative name(s):
Tear lipocalin
Short name=Tlc
Tear prealbumin
Short name=TP
Von Ebner gland protein
Short name=VEG protein

Gene names

Name:	LCN1
Synonyms:	VEGP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket.
Subunit structure	Homodimer By similarity. Binds to LMBR1L which may mediate its endocytosis. Ref.9 Ref.10
Subcellular location	Secreted.
Tissue specificity	Mainly expressed in lachrymal and salivary glands. Also expressed in the prostate.
Sequence similarities	Belongs to the calycin superfamily. Lipocalin family.

Ontologies

Keywords
Biological process	Sensory transduction Taste Transport
Cellular component	Secreted
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Traceable author statement PubMed 8999869. Source: ProtInc response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of taste Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type endopeptidase inhibitor activity Traceable author statement PubMed 8999869. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Ref.8

Chain

19 – 176

158

Lipocalin-1

PRO_0000017974

Amino acid modifications

Disulfide bond

79 ↔ 171

Secondary structure

1

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176

Helix Strand Turn

Details...

Beta strand

33 – 42

10

Beta strand

51 – 54

4

Beta strand

57 – 61

5

Beta strand

67 – 73

7

Beta strand

80 – 88

9

Beta strand

94 – 97

4

Helix

98 – 100

3

Beta strand

102 – 108

7

Beta strand

114 – 121

8

Beta strand

128 – 139

12

Helix

145 – 154

10

Beta strand

160 – 163

4

Sequences

	Sequence		Length	Mass (Da)
	P31025 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 0DDBF124C8C78CB8	FASTA	176	19,250
	10 20 30 40 50 60 MKPLLLAVSL GLIAALQAHH LLASDEEIQD VSGTWYLKAM TVDREFPEMN LESVTPMTLT 70 80 90 100 110 120 TLEGGNLEAK VTMLISGRCQ EVKAVLEKTD EPGKYTADGG KHVAYIIRSH VKDHYIFYCE 130 140 150 160 170 GELHGKPVRG VKLVGRDPKN NLEALEDFEK AAGARGLSTE SILIPRQSET CSPGSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of human von Ebner's gland protein, a member of the lipocalin superfamily highly expressed in lingual salivary glands." Blaeker M., Kock K., Ahlers C., Buck F., Schmale H. Biochim. Biophys. Acta 1172:131-137(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Tongue.
[2]	"cDNA cloning and sequencing reveals human tear prealbumin to be a member of the lipophilic-ligand carrier protein superfamily." Redl B., Holzfeind P., Lottspeich F. J. Biol. Chem. 267:20282-20287(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Tear.
[3]	"Cloning of a human lacrimal lipocalin secreted in tears." Lassagne H., Gachon A.-M. Exp. Eye Res. 56:605-609(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Tear.
[4]	"Structural organization of the gene encoding the human lipocalin tear prealbumin and synthesis of the recombinant protein in Escherichia coli." Holzfeind P., Redl B. Gene 139:177-183(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"Expression of a lipocalin in human nasal mucosa." Scalfari F., Castagna M., Fattori B., Andreini I., Maremmani C., Pelosi P. Comp. Biochem. Physiol. 118B:819-824(1997) Cited for: PROTEIN SEQUENCE OF 19-38. Tissue: Nasal mucus.
[9]	"Molecular cloning of a novel lipocalin-1 interacting human cell membrane receptor using phage display." Wojnar P., Lechner M., Merschak P., Redl B. J. Biol. Chem. 276:20206-20212(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LMBR1L. Tissue: Pituitary.
[10]	"Antisense down-regulation of lipocalin-interacting membrane receptor expression inhibits cellular internalization of lipocalin-1 in human NT2 cells." Wojnar P., Lechner M., Redl B. J. Biol. Chem. 278:16209-16215(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LMBR1L, ENDOCYTOSIS.
[11]	"The 1.8-A crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligands." Breustedt D.A., Korndorfer I.P., Redl B., Skerra A. J. Biol. Chem. 280:484-493(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 23-175.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X62418 mRNA. Translation: CAA44284.1.
M90424 mRNA. Translation: AAA61845.1.
X67647 mRNA. Translation: CAA47889.1.
L14927 Genomic DNA. Translation: AAA18633.1.
AL161452 Genomic DNA. Translation: CAI14045.1.
CH471090 Genomic DNA. Translation: EAW88156.1.
BC065721 mRNA. Translation: AAH65721.1.
BC074925 mRNA. Translation: AAH74925.1.
BC074926 mRNA. Translation: AAH74926.1.

IPI

IPI00009650.

PIR

LCHUL. A44029.

RefSeq

NP_001239546.1. NM_001252617.1.
NP_001239547.1. NM_001252618.1.
NP_001239548.1. NM_001252619.1.
NP_002288.1. NM_002297.3.

UniGene

Hs.530311.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XKI	X-ray	1.80	A	23-175	[»]
3EYC	X-ray	2.60	A/B/C/D	23-175	[»]

ProteinModelPortal

P31025.

ModBase

Search...

Protein-protein interaction databases

IntAct

P31025. 1 interaction.

STRING

9606.ENSP00000263598.

Protein family/group databases

Allergome

3990. Hom s TL.

PTM databases

PhosphoSite

P31025.

Polymorphism databases

DMDM

401346.

2D gel databases

UCD-2DPAGE

P31025.

Proteomic databases

PeptideAtlas

P31025.

PRIDE

P31025.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000263598; ENSP00000263598; ENSG00000160349.
ENST00000371781; ENSP00000360846; ENSG00000160349.

GeneID

3933.

KEGG

hsa:3933.

UCSC

uc004cfz.2. human.

Organism-specific databases

CTD

3933.

GeneCards

GC09P138413.

HGNC

HGNC:6525. LCN1.

MIM

151675. gene.

neXtProt

NX_P31025.

PharmGKB

PA30308.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

HOG000231562.

HOVERGEN

HBG101411.

InParanoid

P31025.

OMA

SGRCQEV.

PhylomeDB

P31025.

Gene expression databases

Bgee

P31025.

CleanEx

HS_LCN1.

Genevestigator

P31025.

GermOnline

ENSG00000160349. Homo sapiens.

Family and domain databases

Gene3D

2.40.128.20. 1 hit.

InterPro

IPR012674. Calycin.
IPR011038. Calycin-like.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
IPR002450. von_Ebner_gland.
[Graphical view]

Pfam

PF00061. Lipocalin. 1 hit.
[Graphical view]

PRINTS

PR01175. VNEBNERGLAND.

SUPFAM

SSF50814. Calycin. 1 hit.

PROSITE

PS00213. LIPOCALIN. False negative.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

LCN1. human.

EvolutionaryTrace

P31025.

GenomeRNAi

3933.

NextBio

15447.

SOURCE

Search...

Entry information

Entry name

LCN1_HUMAN

Accession

Primary (citable) accession number: P31025
Secondary accession number(s): Q5T8A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 129 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.