P31020 (PHEA_PSEUE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenol 2-monooxygenase
EC=1.14.13.7

Alternative name(s):
Phenol hydroxylase

Gene names

Name:

pheA

Encoded on

Plasmid pEST1412

Organism

Pseudomonas sp. (strain EST1001)

Taxonomic identifier

69012 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phenol + NADPH + O2 = catechol + NADP+ + H2O.
Cofactor	FAD.
Pathway	Aromatic compound metabolism; phenol degradation.
Sequence similarities	Belongs to the pheA/tfdB FAD monooxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phenol 2-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 607	607	Phenol 2-monooxygenase		PRO_0000214046
Regions
	Nucleotide binding	37 – 66	30	FAD Potential
	Nucleotide binding	319 – 329	11	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P31020 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: E030D692C96EBAF6	FASTA	607	67,419
	10 20 30 40 50 60 MTTQRNDNLE QPGRSVIFDD GLSATDTPNE TNVVETEVLI VGSGPAGSSA AMFLSTQGIS 70 80 90 100 110 120 NIMITKYRWT ANTPRAHITN QRTMEILRDA GIEDQVLAEA VPHELMGDTV YCESMAGEEI 130 140 150 160 170 180 GRRPTWGTRP DRRADYELAS PAMPCDIPQT LLEPIMLKNA TMRGTQTQFS TEYLSHTQDD 190 200 210 220 230 240 KGVSVQVLNR LTGQEYTIRA KYLIGADGAR SKVAADIGGS MNITFKADLS HWRPSALDPV 250 260 270 280 290 300 LGLPPRIEYR WPRRWFDRMV RPWNEWLVVW GFDINQEPPK LNDDEAIQIV RNLVGIEDLD 310 320 330 340 350 360 VEILGYSLWG NNDQYATHLQ KGRVCCAGDA IHKHPPSHGL GSNTSIQDSY NLCWKLACVL 370 380 390 400 410 420 KGQAGPELLE TYSTERAPIA KQIVTRANGS SSEYKPIFDA LGVTDATTND EFVEKLALRK 430 440 450 460 470 480 ENSPEGARRR AALRAALDNK DYEFNAQGTE IGQFYDSSAV ITDGQKRPAM TEDPMLHHQK 490 500 510 520 530 540 STFPGLRLPH AWLGDAKEKY STHDIAEGTR FTIFTGITGQ AWADAAVRVA ERLGIDLKAV 550 560 570 580 590 600 VIGEGQPVQD LYGDWLRQRE VDEDGVILVR PDKHIGWRAQ SMVADPETAL FDVLSGCCIP SKPALRI

References

[1]	"Sequence of the gene (pheA) encoding phenol monooxygenase from Pseudomonas sp. EST1001: expression in Escherichia coli and Pseudomonas putida." Nurk A., Kasak L., Kivisaar M. Gene 102:13-18(1991) [PubMed: 1650730] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57500 Genomic DNA. No translation available.
PIR	JQ1132.
3D structure databases
ProteinModelPortal	P31020.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002938. mOase_FAD-bd. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01494. FAD_binding_3. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

PHEA_PSEUE

Accession

Primary (citable) accession number: P31020

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	September 21, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program