P31019 (PHEB_PSEUE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catechol 1,2-dioxygenase
EC=1.13.11.1

Alternative name(s):
1,2-CTD

Gene names

Name:

pheB

Encoded on

Plasmid pEST1412

Organism

Pseudomonas sp. (strain EST1001)

Taxonomic identifier

69012 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Catechol + O2 = cis,cis-muconate.
Cofactor	Binds 1 Fe3+ ion per subunit.
Pathway	Aromatic compound metabolism; beta-ketoadipate pathway; 5-oxo-4,5-dihydro-2-furylacetate from catechol: step 1/3.
Sequence similarities	Belongs to the intradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW catechol-containing compound catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	catechol 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC ferric iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Catechol 1,2-dioxygenase		PRO_0000085085
Sites
	Metal binding	164	1	Iron By similarity
	Metal binding	198	1	Iron By similarity
	Metal binding	222	1	Iron By similarity
	Metal binding	224	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31019 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: A86F17E68D1EAC3A	FASTA	302	33,362
	10 20 30 40 50 60 MTVKIYDTPE VQDFLKIVAG LDQEGGNDRG KQIIHRILSD LYRTIDDFDI TAEQYWSAVS 70 80 90 100 110 120 LLNALGQASQ FGLLSPGLGF DHYMDMRMDA ADAEAKRTGG TPRTIEGPLY VAGAPEAEGF 130 140 150 160 170 180 ARMDDDPDTD GETMWLHGQV RDTAGKPIPG AKVEIWHCNS KGGYSFFDKS QTPYNLRRTI 190 200 210 220 230 240 IADNEGYYRA RSVIPSGYGV PEGAPTDQVL KLLGRHGERP AHIHYFISAP GHQHLTTQIN 250 260 270 280 290 300 LAGDPYTYDD FAFATRQDLA AEGKRVENHP AAQQYGVEGT VTEVIFNIEL SPTAEEELQA RP

References

[1]	"Sequence of the plasmid-encoded catechol 1,2-dioxygenase-expressing gene, pheB, of phenol-degrading Pseudomonas sp. strain EST1001." Kivisaar M., Kasak L., Nurk A. Gene 98:15-20(1991) [PubMed: 2013408] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57500 Genomic DNA. Translation: AAC64900.1.
PIR	JN0143.
3D structure databases
ProteinModelPortal	P31019.
SMR	P31019. Positions 3-298.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007535. Catechol_dOase_N. IPR012801. Cchol_dOase_prob. IPR000627. Intradiol_dOase_C. IPR015889. Intradiol_dOase_core. [Graphical view]
Gene3D	G3DSA:2.60.130.10. Intradiol_dOase_core. 1 hit.
Pfam	PF00775. Dioxygenase_C. 1 hit. PF04444. Dioxygenase_N. 1 hit. [Graphical view]
SUPFAM	SSF49482. Dioxygenase. 1 hit.
TIGRFAMs	TIGR02439. Catechol_proteo. 1 hit.
PROSITE	PS00083. INTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHEB_PSEUE

Accession

Primary (citable) accession number: P31019

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	November 16, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program