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Reviewed, UniProtKB/Swiss-Prot P31016 (DLG4_RAT)

Last modified September 2, 2008. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Disks large homolog 4
Alternative name(s):
    Postsynaptic density protein 95
      Short name=PSD-95
    Synapse-associated protein 90
      Short name=SAP90
Gene names
Name: Dlg4
Synonyms: Dlgh4, Psd95
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length724 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.

Subunit structure

Interacts through its first two PDZ domains with KCNA1, KCNA2, KCNA3, KCNA4 and ERBB4. Interacts through its first PDZ domain with GRIK2, KCNA4 and CRIPT. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. May interact with HTR2A. Interacts through its guanylate kinase-like domain with KIF13B. Isoform 2 interacts through an L27 domain with HGS/HRS and the first L27 domain of CASK By similarity. Interacts through its first two PDZ domains with GRIN2A, GRIN2B, GRIN2C, GRIN2D, ACCN3, certain splice forms of GRIN1, KCND2, CXADR and SYNGAP1. Interacts through its second PDZ domain with the PDZ domain of NOS1 or the C-terminus of CAPON. Interacts through its guanylate kinase-like domain with DLGAP1/GKAP, DLGAP2, DLGAP3, DLGAP4, MAP1A and BEGAIN. Interacts through its third PDZ domain with CRIPT. Interacts with ANKS1B, LRFN1 and PRR7.

Subcellular location

Cell membrane; Peripheral membrane protein. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell junction › synapse. Note= High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.

Tissue specificity

Brain. Highest levels of isoform 2 in cerebellum, cortex, hippocampus, and corpus striatum.

Domain

The PDZ domain 3 mediates interaction with ADR1B By similarity.

The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to post-synaptic density By similarity.

Post-translational modification

Palmitoylation of isoform 1 is required for targeting to postsynaptic density.

Sequence similarities

Belongs to the MAGUK family.

Contains 1 guanylate kinase-like domain.

Contains 3 PDZ (DHR) domains.

Contains 1 SH3 domain.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]
Isoform 1 (identifier: P31016-1)

Also known as: PSD95-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P31016-2)

Also known as: PSD95-beta;

The sequence of this isoform is not available.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 724724Disks large homolog 4

Regions

Domain65 – 15187PDZ 1
Domain160 – 24687PDZ 2
Domain313 – 39381PDZ 3
Domain428 – 49871SH3
Domain534 – 709176Guanylate kinase-like

Amino acid modifications

Modified residue1421Phosphoserine By similarity
Modified residue2401Phosphotyrosine By similarity
Modified residue2951Phosphoserine By similarity
Modified residue3971Phosphotyrosine By similarity
Modified residue4151Phosphoserine By similarity
Modified residue4181Phosphoserine By similarity
Modified residue4321Phosphotyrosine By similarity
Modified residue5801Phosphotyrosine By similarity
Modified residue6041Phosphotyrosine By similarity
Modified residue7011Phosphotyrosine By similarity
Modified residue7151Phosphotyrosine By similarity
Lipidation31S-palmitoyl cysteine
Lipidation51S-palmitoyl cysteine

Experimental info

Mutagenesis31C → S: Loss of palmitoylation and targeting to postsynaptic density
Mutagenesis51C → S: Loss of palmitoylation and targeting to postsynaptic density
Sequence conflict611M → L in CAA47103. Ref.2
Sequence conflict781S → T in CAA47103. Ref.2
Sequence conflict177 – 1826GVGNQH → ALGTSI in CAA47103. Ref.2
Sequence conflict2001A → G in CAA47103. Ref.2
Sequence conflict2541S → T in CAA47103. Ref.2
Sequence conflict540 – 55516LGPTK…LLSEF → SLDPPKTVPTMIFSPSS in CAA47103. Ref.2
Sequence conflict623 – 6253GKH → RDQ Ref.4

Secondary structure

1 .................................................................................................... 724
Helix Strand Turn

Details...