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Protein

Tyrosine phenol-lyase

Gene

tpl

Organism
Citrobacter freundii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-tyrosine + H2O = phenol + pyruvate + NH3.

Cofactori

GO - Molecular functioni

  • tyrosine phenol-lyase activity Source: CACAO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi4.1.99.2. 1398.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine phenol-lyase (EC:4.1.99.2)
Alternative name(s):
Beta-tyrosinase
Gene namesi
Name:tpl
OrganismiCitrobacter freundii
Taxonomic identifieri546 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001956311 – 456Tyrosine phenol-lyaseAdd BLAST456

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei257N6-(pyridoxal phosphate)lysine1

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1456
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 15Combined sources9
Helixi21 – 30Combined sources10
Turni31 – 33Combined sources3
Helixi35 – 37Combined sources3
Helixi40 – 42Combined sources3
Beta strandi44 – 46Combined sources3
Beta strandi50 – 52Combined sources3
Helixi58 – 64Combined sources7
Beta strandi71 – 73Combined sources3
Helixi75 – 88Combined sources14
Beta strandi91 – 98Combined sources8
Helixi99 – 110Combined sources12
Beta strandi116 – 121Combined sources6
Helixi124 – 132Combined sources9
Beta strandi136 – 139Combined sources4
Helixi143 – 146Combined sources4
Turni153 – 156Combined sources4
Helixi160 – 170Combined sources11
Helixi172 – 174Combined sources3
Beta strandi175 – 184Combined sources10
Turni185 – 188Combined sources4
Helixi194 – 206Combined sources13
Beta strandi211 – 214Combined sources4
Helixi218 – 228Combined sources11
Helixi237 – 245Combined sources9
Beta strandi249 – 254Combined sources6
Turni255 – 259Combined sources5
Beta strandi265 – 270Combined sources6
Helixi272 – 285Combined sources14
Turni289 – 293Combined sources5
Helixi296 – 309Combined sources14
Helixi312 – 331Combined sources20
Beta strandi342 – 348Combined sources7
Helixi349 – 352Combined sources4
Turni353 – 355Combined sources3
Helixi358 – 360Combined sources3
Helixi362 – 374Combined sources13
Beta strandi375 – 377Combined sources3
Beta strandi379 – 382Combined sources4
Helixi383 – 386Combined sources4
Turni390 – 392Combined sources3
Beta strandi402 – 406Combined sources5
Turni409 – 411Combined sources3
Helixi414 – 429Combined sources16
Helixi430 – 433Combined sources4
Beta strandi437 – 441Combined sources5
Beta strandi444 – 446Combined sources3
Helixi447 – 449Combined sources3
Beta strandi452 – 455Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TPLX-ray2.30A/B1-456[»]
2EZ1X-ray1.90A/B1-456[»]
2EZ2X-ray1.85A/B1-456[»]
2TPLX-ray2.50A/B1-456[»]
2VLFX-ray1.89A/B1-456[»]
2VLHX-ray1.95A/B1-456[»]
2YCNX-ray2.04A/B1-456[»]
2YCPX-ray2.00A/B/C/D1-456[»]
2YCTX-ray2.25A/B1-456[»]
2YHKX-ray1.91A/B1-456[»]
ProteinModelPortaliP31013.
SMRiP31013.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31013.

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-eliminating lyase family.Curated

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00543. Tyr_phenol_lyase. 1 hit.
InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR011166. Beta-eliminating_lyase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR018176. Tryptophanase_CS.
IPR013441. Tyr_phenol_ly.
[Graphical view]
PfamiPF01212. Beta_elim_lyase. 1 hit.
[Graphical view]
PIRSFiPIRSF001386. Trpase. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02618. tyr_phenol_ly. 1 hit.
PROSITEiPS00853. BETA_ELIM_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYPAEPFRI KSVETVSMIP RDERLKKMQE AGYNTFLLNS KDIYIDLLTD
60 70 80 90 100
SGTNAMSDKQ WAGMMMGDEA YAGSENFYHL ERTVQELFGF KHIVPTHQGR
110 120 130 140 150
GAENLLSQLA IKPGQYVAGN MYFTTTRYHQ EKNGAVFVDI VRDEAHDAGL
160 170 180 190 200
NIAFKGDIDL KKLQKLIDEK GAENIAYICL AVTVNLAGGQ PVSMANMRAV
210 220 230 240 250
RELTEAHGIK VFYDATRCVE NAYFIKEQEQ GFENKSIAEI VHEMFSYADG
260 270 280 290 300
CTMSGKKDCL VNIGGFLCMN DDEMFSSAKE LVVVYEGMPS YGGLAGRDME
310 320 330 340 350
AMAIGLREAM QYEYIEHRVK QVRYLGDKLK AAGVPIVEPV GGHAVFLDAR
360 370 380 390 400
RFCEHLTQDE FPAQSLAASI YVETGVRSME RGIISAGRNN VTGEHHRPKL
410 420 430 440 450
ETVRLTIPRR VYTYAHMDVV ADGIIKLYQH KEDIRGLKFI YEPKQLRFFT

ARFDYI
Length:456
Mass (Da):51,500
Last modified:July 1, 1993 - v1
Checksum:iB77AB58233C22474
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti205E → A in AAA23098 (PubMed:7916622).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66978 Genomic DNA. Translation: CAA47388.1.
L10821 Genomic DNA. Translation: AAA23098.1.
PIRiA49493.
RefSeqiWP_003837154.1. NZ_LLJR01000005.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66978 Genomic DNA. Translation: CAA47388.1.
L10821 Genomic DNA. Translation: AAA23098.1.
PIRiA49493.
RefSeqiWP_003837154.1. NZ_LLJR01000005.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TPLX-ray2.30A/B1-456[»]
2EZ1X-ray1.90A/B1-456[»]
2EZ2X-ray1.85A/B1-456[»]
2TPLX-ray2.50A/B1-456[»]
2VLFX-ray1.89A/B1-456[»]
2VLHX-ray1.95A/B1-456[»]
2YCNX-ray2.04A/B1-456[»]
2YCPX-ray2.00A/B/C/D1-456[»]
2YCTX-ray2.25A/B1-456[»]
2YHKX-ray1.91A/B1-456[»]
ProteinModelPortaliP31013.
SMRiP31013.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi4.1.99.2. 1398.

Miscellaneous databases

EvolutionaryTraceiP31013.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00543. Tyr_phenol_lyase. 1 hit.
InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR011166. Beta-eliminating_lyase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR018176. Tryptophanase_CS.
IPR013441. Tyr_phenol_ly.
[Graphical view]
PfamiPF01212. Beta_elim_lyase. 1 hit.
[Graphical view]
PIRSFiPIRSF001386. Trpase. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02618. tyr_phenol_ly. 1 hit.
PROSITEiPS00853. BETA_ELIM_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPL_CITFR
AccessioniPrimary (citable) accession number: P31013
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.