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Protein

Tyrosine phenol-lyase

Gene

tpl

Organism
Enterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-tyrosine + H2O = phenol + pyruvate + NH3.

Cofactori

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine phenol-lyase (EC:4.1.99.2)
Alternative name(s):
Beta-tyrosinase
Gene namesi
Name:tpl
Synonyms:tutA
OrganismiEnterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans)
Taxonomic identifieri549 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaePantoeaPantoea agglomerans group

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001956331 – 456Tyrosine phenol-lyaseAdd BLAST456

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei257N6-(pyridoxal phosphate)lysineBy similarity1

Post-translational modificationi

Contains L-DOPA (3',4'-dihydroxyphenylalanine).

Proteomic databases

PRIDEiP31011.

Expressioni

Inductioni

By L-tyrosine.

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1456
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 15Combined sources9
Helixi21 – 30Combined sources10
Turni31 – 33Combined sources3
Helixi35 – 37Combined sources3
Helixi40 – 42Combined sources3
Beta strandi44 – 46Combined sources3
Beta strandi50 – 52Combined sources3
Helixi58 – 63Combined sources6
Beta strandi71 – 73Combined sources3
Helixi75 – 88Combined sources14
Beta strandi91 – 98Combined sources8
Helixi99 – 110Combined sources12
Beta strandi115 – 121Combined sources7
Helixi124 – 132Combined sources9
Beta strandi135 – 139Combined sources5
Helixi143 – 146Combined sources4
Helixi160 – 170Combined sources11
Helixi172 – 174Combined sources3
Beta strandi175 – 184Combined sources10
Turni185 – 188Combined sources4
Helixi194 – 207Combined sources14
Beta strandi211 – 214Combined sources4
Helixi218 – 228Combined sources11
Helixi237 – 245Combined sources9
Beta strandi249 – 254Combined sources6
Turni255 – 259Combined sources5
Beta strandi265 – 270Combined sources6
Helixi272 – 285Combined sources14
Turni289 – 293Combined sources5
Helixi296 – 309Combined sources14
Helixi312 – 331Combined sources20
Beta strandi342 – 348Combined sources7
Helixi349 – 352Combined sources4
Helixi358 – 360Combined sources3
Helixi362 – 374Combined sources13
Beta strandi375 – 377Combined sources3
Beta strandi379 – 382Combined sources4
Helixi383 – 386Combined sources4
Beta strandi390 – 392Combined sources3
Beta strandi402 – 406Combined sources5
Helixi414 – 428Combined sources15
Helixi429 – 433Combined sources5
Beta strandi437 – 441Combined sources5
Beta strandi444 – 446Combined sources3
Helixi447 – 450Combined sources4
Beta strandi452 – 455Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C7GX-ray2.10A/B/C/D1-456[»]
ProteinModelPortaliP31011.
SMRiP31011.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31011.

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-eliminating lyase family.Curated

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00543. Tyr_phenol_lyase. 1 hit.
InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR011166. Beta-eliminating_lyase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR018176. Tryptophanase_CS.
IPR013441. Tyr_phenol_ly.
[Graphical view]
PfamiPF01212. Beta_elim_lyase. 1 hit.
[Graphical view]
PIRSFiPIRSF001386. Trpase. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02618. tyr_phenol_ly. 1 hit.
PROSITEiPS00853. BETA_ELIM_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYPAEPFRI KSVETVSMIS RDERVKKMQE AGYNTFLLNS KDIYIDLLTD
60 70 80 90 100
SGTNAMSDKQ WAGMMIGDEA YAGSENFYHL EKTVKELFGF KHIVPTHQGR
110 120 130 140 150
GAENLLSQLA IKPGQYVAGN MYFTTTRFHQ EKNGATFVDI VRDEAHDASL
160 170 180 190 200
NLPFKGDIDL NKLATLIKEK GAENIAYICL AVTVNLAGGQ PVSMANMRAV
210 220 230 240 250
HEMASTYGIK IFYDATRCVE NAYFIKEQEA GYENVSIKDI VHEMFSYADG
260 270 280 290 300
CTMSGKKDCL VNIGGFLCMN DEEMFSAAKE LVVVYEGMPS YGGLAGRDME
310 320 330 340 350
AMAIGLREAM QYEYIEHRVK QVRYLGDKLR EAGVPIVEPT GGHAVFLDAR
360 370 380 390 400
RFCPHLTQDQ FPAQSLAASI YMETGVRSME RGIVSAGRSK ETGENHRPKL
410 420 430 440 450
ETVRLTIPRR VYTYAHMDVV ADGIIKLYQH KEDIRGLTFV YEPKQLRFFT

ARFDFI
Length:456
Mass (Da):51,365
Last modified:February 1, 1996 - v2
Checksum:i34758B48AF7C4AE2
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti157D → N in strain: MT-10509. 1
Natural varianti212F → Y in strain: MT-10509. 1
Natural varianti397R → S in strain: MT-10509. 1
Natural varianti420V → I in strain: MT-10509. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S50513 Genomic DNA. Translation: AAB24234.1.
D13714 Genomic DNA. Translation: BAA02867.1.
U25347 Genomic DNA. Translation: AAA66390.1.
L08484 Genomic DNA. Translation: AAA71928.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S50513 Genomic DNA. Translation: AAB24234.1.
D13714 Genomic DNA. Translation: BAA02867.1.
U25347 Genomic DNA. Translation: AAA66390.1.
L08484 Genomic DNA. Translation: AAA71928.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C7GX-ray2.10A/B/C/D1-456[»]
ProteinModelPortaliP31011.
SMRiP31011.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP31011.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP31011.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00543. Tyr_phenol_lyase. 1 hit.
InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR011166. Beta-eliminating_lyase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR018176. Tryptophanase_CS.
IPR013441. Tyr_phenol_ly.
[Graphical view]
PfamiPF01212. Beta_elim_lyase. 1 hit.
[Graphical view]
PIRSFiPIRSF001386. Trpase. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02618. tyr_phenol_ly. 1 hit.
PROSITEiPS00853. BETA_ELIM_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPL_ENTAG
AccessioniPrimary (citable) accession number: P31011
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.