P31006 (KGUA_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanylate kinase
EC=2.7.4.8

Alternative name(s):
GMP kinase

Gene names

Name:	GUK1
Synonyms:	GUK

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for recycling GMP and indirectly, cGMP.
Catalytic activity	ATP + GMP = ADP + GDP.
Subunit structure	Monomer.
Sequence similarities	Belongs to the guanylate kinase family. Contains 1 guanylate kinase-like domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	purine nucleotide metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW guanylate kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 198	197	Guanylate kinase		PRO_0000170653
Regions
	Domain	4 – 186	183	Guanylate kinase-like
	Nucleotide binding	11 – 18	8	ATP By similarity
Sites
	Active site	44	1	By similarity
	Active site	137	1	By similarity
	Active site	148	1	By similarity
	Binding site	171	1	ATP By similarity
	Binding site	172	1	ATP; via carbonyl oxygen By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylglycine Ref.1
	Modified residue	53	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31006 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 09E9CC7130CCE1A7	FASTA	198	21,921
	10 20 30 40 50 60 MGSPRPVVLS GPSGAGKSTL LKKLLQEHSS IFGFSVSHTT RDPRPGEENG KDYYFVTREV 70 80 90 100 110 120 MQRDIAAGDF IEHAEFSGNL YGTSKAAVRA VQAMNRICVL DVDLQGVRNI KKTDLQPIYI 130 140 150 160 170 180 FVQPPSLDVL EQRLRQRNTE TEESLAKRLA AAKADMESSK EPGLFDLIII NDSLDKAYWA 190 LKEALSEEIK KAQATGHS

References

[1]	"Purification and sequence determination of guanylate kinase from pig brain." Zschocke P.D., Schiltz E., Schulz G.E. Eur. J. Biochem. 213:263-269(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-198. Tissue: Brain.

Cross-references

Sequence databases
PIR	KIPGGU. S23776.
3D structure databases
ProteinModelPortal	P31006.
SMR	P31006. Positions 5-194.
ModBase	Search...
Protein-protein interaction databases
STRING	9823.ENSSSCP00000014875.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG0194.
HOGENOM	HOG000037640.
HOVERGEN	HBG003344.
OrthoDB	EOG4NS3CS.
Enzyme and pathway databases
Reactome	REACT_114137. Metabolism.
Family and domain databases
InterPro	IPR008144. Guanylate_kin. IPR008145. Guanylate_kin/L-typ_Ca_channel. IPR020590. Guanylate_kinase_CS. IPR017665. Guanylate_kinase_sub. [Graphical view]
Pfam	PF00625. Guanylate_kin. 1 hit. [Graphical view]
SMART	SM00072. GuKc. 1 hit. [Graphical view]
TIGRFAMs	TIGR03263. guanyl_kin. 1 hit.
PROSITE	PS00856. GUANYLATE_KINASE_1. 1 hit. PS50052. GUANYLATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KGUA_PIG

Accession

Primary (citable) accession number: P31006

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program