Reviewed, UniProtKB/Swiss-Prot P31005 (MEDH_BACMT)

Names and origin

Protein names

Recommended name:
    NAD-dependent methanol dehydrogenase
      Short name=MEDH
    EC=1.1.1.244

Gene names

Name:

mdh

Organism

Bacillus methanolicus

Taxonomic identifier

1471 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Methanol + NAD+ = formaldehyde + NADH.
Pathway	One-carbon metabolism; methanol degradation; formaldehyde from methanol: step 1/1.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: InterPro methanol dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 381	380	NAD-dependent methanol dehydrogenase		PRO_0000087834
Experimental info
	Sequence conflict	18	1	K → L AA sequence Ref.2
	Sequence conflict	42	1	S → A AA sequence Ref.2
	Sequence conflict	50	1	A → D AA sequence Ref.2
	Sequence conflict	59	1	D → N AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P31005-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8EA251B36F73749D	FASTA	381	40,045
	10 20 30 40 50 60 MTNFFIPPAS VIGRGAVKEV GTRLKQIGAK KALIVTDAFL HSTGLSEEVA KNIREAGLDV 70 80 90 100 110 120 AIFPKAQPDP ADTQVHEGVD VFKQENCDAL VSIGGGSSHD TAKAIGLVAA NGGRINDYQG 130 140 150 160 170 180 VNSVEKPVVP VVAITTTAGT GSETTSLAVI TDSARKVKMP VIDEKITPTV AIVDPELMVK 190 200 210 220 230 240 KPAGLTIATG MDALSHAIEA YVAKGATPVT DAFAIQAMKL INEYLPKAVA NGEDIEAREA 250 260 270 280 290 300 MAYAQYMAGV AFNNGGLGLV HSISHQVGGV YKLQHGICNS VNMPHVCAFN LIAKTERFAH 310 320 330 340 350 360 IAELLGENVS GLSTAAAAER AIVALERYNK NFGIPSGYAE MGVKEEDIEL LAKNAFEDVC 370 380 TQSNPRVATV QDIAQIIKNA L

References

[1]	"Cloning, expression, and sequence analysis of the Bacillus methanolicus C1 methanol dehydrogenase gene." de Vries G.E., Arfman N., Terpstra P., Dijkhuizen L. J. Bacteriol. 174:5346-5353(1992) [PubMed: 1644761] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C1.
[2]	"Electron microscopic analysis and biochemical characterization of a novel methanol dehydrogenase from the thermotolerant Bacillus sp. C1." Vonck J., Arfman N., de Vries G.E., van Beeumen J., van Bruggen E.F.J., Dijkhuizen L. J. Biol. Chem. 266:3949-3954(1991) [PubMed: 1995642] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-61. Strain: C1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M65004 Genomic DNA. Translation: AAA22593.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.244. 2609.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MEDH_BACMT

Accession

Primary (citable) accession number: P31005

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 45 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)