Reviewed, UniProtKB/Swiss-Prot P31003 (PHEB_BACST)

Names and origin

Protein names

Recommended name:
    Metapyrocatechase
      Short name=MPC
    EC=1.13.11.2
Alternative name(s):
    CatO2ase
    Catechol 2,3-dioxygenase

Gene names

Name:

pheB

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Catechol + O2 = 2-hydroxymuconate semialdehyde.
Cofactor	Fe2+ ion.
Sequence similarities	Belongs to the extradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Metapyrocatechase		PRO_0000085025
Sites
	Metal binding	159	1	Iron By similarity
	Metal binding	221	1	Iron By similarity
	Metal binding	272	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31003-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: F8E7550697798EA7	FASTA	327	37,419
	10 20 30 40 50 60 MSKNFQEPIF DVAQLAHVEL LSPKLEESIV FFTKYLGMEV TARAGNSVYL RAYEDFYHNT 70 80 90 100 110 120 LKITESAEAG LGHVGWRASS PQALERRVLE LEKSGLGRGW IDGDIGHGKA YQFTTPDGHQ 130 140 150 160 170 180 MEIFFEVEYY KPQPEQKTKL LNRPSKRPAQ GVPVRRLDHI NLMTSNPGVD TQFMIDTLGF 190 200 210 220 230 240 RLREQIRDKG KILGSWISVS NLVHEIAFMQ EPNQEKGKLH HLCYWYGIPQ NLYDLADLLK 250 260 270 280 290 300 DHEYFIEVPP NKHGISQAFC MYVYEPGGNR IELFGDAGYL ITDPTWEPVI WEMEDVPGNG 310 320 DTWIGTAFPD SWWLRGTPVT TKEVVKP

References

[1]	He Z.-Q., Mao Y.-M., Sheng Z.-J., Shen R.-Q. Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FDTP-3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X67860 Genomic DNA. Translation: CAA48044.1.
PIR	JC1324.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.11.2. 266715.
Family and domain databases
InterPro	IPR017624. Catechol_2-3_dOase. IPR004360. Glyas_bleo-R_dOase. IPR000486. Xdiol_dOase_1_2. [Graphical view]
Pfam	PF00903. Glyoxalase. 2 hits. [Graphical view]
ProDom	PD002334. Gly_diox. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03211. catechol_2_3. 1 hit.
PROSITE	PS00082. EXTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHEB_BACST

Accession

Primary (citable) accession number: P31003

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)