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P30994

- 5HT2B_RAT

UniProt

P30994 - 5HT2B_RAT

Protein

5-hydroxytryptamine receptor 2B

Gene

Htr2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. Plays a role in the regulation of behavior, including impulsive behavior. Required for normal proliferation of embryonic cardiac myocytes and normal heart development. Protects cardiomyocytes against apoptosis. Plays a role in the adaptation of pulmonary arteries to chronic hypoxia. Plays a role in vasoconstriction. Required for normal osteoblast function and proliferation, and for maintaining normal bone density. Required for normal proliferation of the interstitial cells of Cajal in the intestine By similarity. May play a role in the perception of pain.By similarity2 Publications

    GO - Molecular functioni

    1. drug binding Source: UniProtKB
    2. G-protein alpha-subunit binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. Ras GTPase activator activity Source: UniProtKB
    5. serotonin binding Source: UniProtKB
    6. serotonin receptor activity Source: UniProtKB

    GO - Biological processi

    1. behavior Source: UniProtKB-KW
    2. calcium-mediated signaling Source: UniProtKB
    3. cardiac muscle hypertrophy Source: UniProtKB
    4. cellular response to temperature stimulus Source: UniProtKB
    5. cGMP biosynthetic process Source: UniProtKB
    6. embryonic morphogenesis Source: UniProtKB
    7. ERK1 and ERK2 cascade Source: UniProtKB
    8. G-protein coupled receptor signaling pathway Source: UniProtKB
    9. heart morphogenesis Source: UniProtKB
    10. intestine smooth muscle contraction Source: UniProtKB
    11. negative regulation of apoptotic process Source: UniProtKB
    12. negative regulation of autophagy Source: UniProtKB
    13. negative regulation of cell death Source: UniProtKB
    14. neural crest cell differentiation Source: UniProtKB
    15. neural crest cell migration Source: UniProtKB
    16. phosphatidylinositol 3-kinase signaling Source: UniProtKB
    17. phosphorylation Source: UniProtKB
    18. positive regulation of cell division Source: UniProtKB
    19. positive regulation of cell proliferation Source: UniProtKB
    20. positive regulation of cytokine production Source: UniProtKB
    21. positive regulation of cytokine secretion Source: UniProtKB
    22. positive regulation of endothelial cell proliferation Source: UniProtKB
    23. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
    24. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
    25. positive regulation of nitric-oxide synthase activity Source: UniProtKB
    26. positive regulation of phosphatidylinositol biosynthetic process Source: UniProtKB
    27. positive regulation of Ras GTPase activity Source: GOC
    28. protein kinase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
    29. protein kinase C signaling Source: UniProtKB
    30. regulation of behavior Source: UniProtKB
    31. release of sequestered calcium ion into cytosol Source: UniProtKB
    32. response to drug Source: UniProtKB
    33. serotonin receptor signaling pathway Source: UniProtKB
    34. vasoconstriction Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Behavior

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-hydroxytryptamine receptor 2B
    Short name:
    5-HT-2B
    Short name:
    5-HT2B
    Alternative name(s):
    5-HT-2F
    Serotonin receptor 2B
    Stomach fundus serotonin receptor
    Gene namesi
    Name:Htr2b
    Synonyms:Srl
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi61801. Htr2b.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell junctionsynapsesynaptosome By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cytoplasm Source: UniProtKB
    3. integral component of plasma membrane Source: InterPro
    4. neuron projection Source: UniProtKB-SubCell
    5. plasma membrane Source: UniProtKB
    6. synapse Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Synapse, Synaptosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4794795-hydroxytryptamine receptor 2BPRO_0000068956Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi127 ↔ 206PROSITE-ProRule annotation
    Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi349 ↔ 352PROSITE-ProRule annotation
    Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence Analysis
    Lipidationi396 – 3961S-palmitoyl cysteineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PRIDEiP30994.

    PTM databases

    PhosphoSiteiP30994.

    Expressioni

    Tissue specificityi

    Stomach fundus.1 Publication

    Gene expression databases

    GenevestigatoriP30994.

    Interactioni

    Subunit structurei

    Interacts with MPDZ.By similarity

    Protein-protein interaction databases

    BioGridi248214. 1 interaction.
    IntActiP30994. 1 interaction.
    MINTiMINT-4000039.
    STRINGi10116.ENSRNOP00000023829.

    Structurei

    3D structure databases

    ProteinModelPortaliP30994.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5555ExtracellularBy similarityAdd
    BLAST
    Topological domaini79 – 8911CytoplasmicBy similarityAdd
    BLAST
    Topological domaini113 – 12816ExtracellularBy similarityAdd
    BLAST
    Topological domaini151 – 17020CytoplasmicBy similarityAdd
    BLAST
    Topological domaini192 – 21524ExtracellularBy similarityAdd
    BLAST
    Topological domaini239 – 32385CytoplasmicBy similarityAdd
    BLAST
    Topological domaini345 – 35915ExtracellularBy similarityAdd
    BLAST
    Topological domaini382 – 47998CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei56 – 7823Helical; Name=1By similarityAdd
    BLAST
    Transmembranei90 – 11223Helical; Name=2By similarityAdd
    BLAST
    Transmembranei129 – 15022Helical; Name=3By similarityAdd
    BLAST
    Transmembranei171 – 19121Helical; Name=4By similarityAdd
    BLAST
    Transmembranei216 – 23823Helical; Name=5By similarityAdd
    BLAST
    Transmembranei324 – 34421Helical; Name=6By similarityAdd
    BLAST
    Transmembranei360 – 38122Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni134 – 1396Agonist bindingBy similarity
    Regioni336 – 3405Agonist bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi151 – 1533DRY motif; important for ligand-induced conformation changesBy similarity
    Motifi211 – 2144[DE]RFG motif; may stabilize a conformation that preferentially activates signaling via beta-arrestin family membersBy similarity
    Motifi375 – 3795NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity
    Motifi477 – 4793PDZ-binding

    Domaini

    Ligands are bound in a hydrophobic pocket formed by the transmembrane helices.By similarity

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG247243.
    HOGENOMiHOG000240378.
    HOVERGENiHBG107487.
    InParanoidiP30994.
    KOiK04157.
    PhylomeDBiP30994.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR000482. 5HT2B_rcpt.
    IPR002231. 5HT_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PANTHERiPTHR24247:SF31. PTHR24247:SF31. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00651. 5HT2BRECEPTR.
    PR01101. 5HTRECEPTOR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30994-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASSYKMSEQ STISEHILQK TCDHLILTDR SGLKAESAAE EMKQTAENQG    50
    NTVHWAALLI FAVIIPTIGG NILVILAVSL EKRLQYATNY FLMSLAVADL 100
    LVGLFVMPIA LLTIMFEATW PLPLALCPAW LFLDVLFSTA SIMHLCAISL 150
    DRYIAIKKPI QANQCNSRTT AFVKITVVWL ISIGIAIPVP IKGIEADVVN 200
    AHNITCELTK DRFGSFMLFG SLAAFFAPLT IMIVTYFLTI HALRKKAYLV 250
    RNRPPQRLTR WTVSTVLQRE DSSFSSPEKM VMLDGSHKDK ILPNSTDETL 300
    MRRMSSAGKK PAQTISNEQR ASKVLGIVFL FFLLMWCPFF ITNVTLALCD 350
    SCNQTTLKTL LQIFVWVGYV SSGVNPLIYT LFNKTFREAF GRYITCNYQA 400
    TKSVKVLRKC SSTLYFGNSM VENSKFFTKH GIRNGINPAM YQSPVRLRSS 450
    TIQSSSIILL NTFLTENDGD KVEDQVSYI 479
    Length:479
    Mass (Da):53,652
    Last modified:July 1, 1993 - v1
    Checksum:i17FFC73213B42038
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti281 – 2811V → A(PubMed:1331748)Curated
    Sequence conflicti296 – 2961T → I no nucleotide entry (PubMed:1331748)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66842 mRNA. Translation: CAA47318.1.
    PIRiS23562.
    RefSeqiNP_058946.1. NM_017250.1.
    UniGeneiRn.10425.

    Genome annotation databases

    GeneIDi29581.
    KEGGirno:29581.
    UCSCiRGD:61801. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66842 mRNA. Translation: CAA47318.1 .
    PIRi S23562.
    RefSeqi NP_058946.1. NM_017250.1.
    UniGenei Rn.10425.

    3D structure databases

    ProteinModelPortali P30994.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248214. 1 interaction.
    IntActi P30994. 1 interaction.
    MINTi MINT-4000039.
    STRINGi 10116.ENSRNOP00000023829.

    Chemistry

    BindingDBi P30994.
    ChEMBLi CHEMBL2111398.
    GuidetoPHARMACOLOGYi 7.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P30994.

    Proteomic databases

    PRIDEi P30994.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 29581.
    KEGGi rno:29581.
    UCSCi RGD:61801. rat.

    Organism-specific databases

    CTDi 3357.
    RGDi 61801. Htr2b.

    Phylogenomic databases

    eggNOGi NOG247243.
    HOGENOMi HOG000240378.
    HOVERGENi HBG107487.
    InParanoidi P30994.
    KOi K04157.
    PhylomeDBi P30994.

    Miscellaneous databases

    NextBioi 609690.

    Gene expression databases

    Genevestigatori P30994.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR000482. 5HT2B_rcpt.
    IPR002231. 5HT_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    PANTHERi PTHR24247:SF31. PTHR24247:SF31. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00651. 5HT2BRECEPTR.
    PR01101. 5HTRECEPTOR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and functional characterization of the rat stomach fundus serotonin receptor."
      Foguet M., Hoyer D., Pardo L.A., Parekh A., Kluxen F.-W., Kalkman M.O., Stuehmer W., Luebbert H.
      EMBO J. 11:3481-3487(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
    2. "Molecular cloning, functional expression, and pharmacological characterization of a novel serotonin receptor (5-hydroxytryptamine2F) from rat stomach fundus."
      Kursar J.D., Nelson D.L., Wainscott D.B., Cohen M.L., Baez M.
      Mol. Pharmacol. 42:549-557(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Gastric fundus.
    3. "Antinociceptive effect of peripheral serotonin 5-HT2B receptor activation on neuropathic pain."
      Urtikova N., Berson N., Van Steenwinckel J., Doly S., Truchetto J., Maroteaux L., Pohl M., Conrath M.
      Pain 153:1320-1331(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry namei5HT2B_RAT
    AccessioniPrimary (citable) accession number: P30994
    Secondary accession number(s): Q9QW44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3