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Protein

5-hydroxytryptamine receptor 2B

Gene

Htr2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. Plays a role in the regulation of behavior, including impulsive behavior. Required for normal proliferation of embryonic cardiac myocytes and normal heart development. Protects cardiomyocytes against apoptosis. Plays a role in the adaptation of pulmonary arteries to chronic hypoxia. Plays a role in vasoconstriction. Required for normal osteoblast function and proliferation, and for maintaining normal bone density. Required for normal proliferation of the interstitial cells of Cajal in the intestine (By similarity). May play a role in the perception of pain.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 2B
Short name:
5-HT-2B
Short name:
5-HT2B
Alternative name(s):
5-HT-2F
Serotonin receptor 2B
Stomach fundus serotonin receptor
Gene namesi
Name:Htr2b
Synonyms:Srl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61801. Htr2b.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 55ExtracellularBy similarityAdd BLAST55
Transmembranei56 – 78Helical; Name=1By similarityAdd BLAST23
Topological domaini79 – 89CytoplasmicBy similarityAdd BLAST11
Transmembranei90 – 112Helical; Name=2By similarityAdd BLAST23
Topological domaini113 – 128ExtracellularBy similarityAdd BLAST16
Transmembranei129 – 150Helical; Name=3By similarityAdd BLAST22
Topological domaini151 – 170CytoplasmicBy similarityAdd BLAST20
Transmembranei171 – 191Helical; Name=4By similarityAdd BLAST21
Topological domaini192 – 215ExtracellularBy similarityAdd BLAST24
Transmembranei216 – 238Helical; Name=5By similarityAdd BLAST23
Topological domaini239 – 323CytoplasmicBy similarityAdd BLAST85
Transmembranei324 – 344Helical; Name=6By similarityAdd BLAST21
Topological domaini345 – 359ExtracellularBy similarityAdd BLAST15
Transmembranei360 – 381Helical; Name=7By similarityAdd BLAST22
Topological domaini382 – 479CytoplasmicBy similarityAdd BLAST98

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • dendrite Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • membrane Source: RGD
  • neuronal cell body Source: RGD
  • plasma membrane Source: UniProtKB
  • synapse Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL323.
GuidetoPHARMACOLOGYi7.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000689561 – 4795-hydroxytryptamine receptor 2BAdd BLAST479

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi127 ↔ 206PROSITE-ProRule annotation
Glycosylationi203N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi349 ↔ 352PROSITE-ProRule annotation
Glycosylationi353N-linked (GlcNAc...)Sequence analysis1
Lipidationi396S-palmitoyl cysteineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP30994.
PRIDEiP30994.

PTM databases

PhosphoSitePlusiP30994.

Expressioni

Tissue specificityi

Stomach fundus.1 Publication

Interactioni

Subunit structurei

Interacts with MPDZ.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248214. 1 interactor.
IntActiP30994. 1 interactor.
MINTiMINT-4000039.
STRINGi10116.ENSRNOP00000023829.

Chemistry databases

BindingDBiP30994.

Structurei

3D structure databases

ProteinModelPortaliP30994.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 139Agonist bindingBy similarity6
Regioni336 – 340Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi151 – 153DRY motif; important for ligand-induced conformation changesBy similarity3
Motifi211 – 214[DE]RFG motif; may stabilize a conformation that preferentially activates signaling via beta-arrestin family membersBy similarity4
Motifi375 – 379NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity5
Motifi477 – 479PDZ-binding3

Domaini

Ligands are bound in a hydrophobic pocket formed by the transmembrane helices.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000240378.
HOVERGENiHBG107487.
InParanoidiP30994.
KOiK04157.
PhylomeDBiP30994.

Family and domain databases

InterProiIPR000482. 5HT2B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF31. PTHR24247:SF31. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00651. 5HT2BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30994-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSYKMSEQ STISEHILQK TCDHLILTDR SGLKAESAAE EMKQTAENQG
60 70 80 90 100
NTVHWAALLI FAVIIPTIGG NILVILAVSL EKRLQYATNY FLMSLAVADL
110 120 130 140 150
LVGLFVMPIA LLTIMFEATW PLPLALCPAW LFLDVLFSTA SIMHLCAISL
160 170 180 190 200
DRYIAIKKPI QANQCNSRTT AFVKITVVWL ISIGIAIPVP IKGIEADVVN
210 220 230 240 250
AHNITCELTK DRFGSFMLFG SLAAFFAPLT IMIVTYFLTI HALRKKAYLV
260 270 280 290 300
RNRPPQRLTR WTVSTVLQRE DSSFSSPEKM VMLDGSHKDK ILPNSTDETL
310 320 330 340 350
MRRMSSAGKK PAQTISNEQR ASKVLGIVFL FFLLMWCPFF ITNVTLALCD
360 370 380 390 400
SCNQTTLKTL LQIFVWVGYV SSGVNPLIYT LFNKTFREAF GRYITCNYQA
410 420 430 440 450
TKSVKVLRKC SSTLYFGNSM VENSKFFTKH GIRNGINPAM YQSPVRLRSS
460 470
TIQSSSIILL NTFLTENDGD KVEDQVSYI
Length:479
Mass (Da):53,652
Last modified:July 1, 1993 - v1
Checksum:i17FFC73213B42038
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti281V → A (PubMed:1331748).Curated1
Sequence conflicti296T → I no nucleotide entry (PubMed:1331748).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66842 mRNA. Translation: CAA47318.1.
PIRiS23562.
RefSeqiNP_058946.1. NM_017250.1.
UniGeneiRn.10425.

Genome annotation databases

GeneIDi29581.
KEGGirno:29581.
UCSCiRGD:61801. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66842 mRNA. Translation: CAA47318.1.
PIRiS23562.
RefSeqiNP_058946.1. NM_017250.1.
UniGeneiRn.10425.

3D structure databases

ProteinModelPortaliP30994.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248214. 1 interactor.
IntActiP30994. 1 interactor.
MINTiMINT-4000039.
STRINGi10116.ENSRNOP00000023829.

Chemistry databases

BindingDBiP30994.
ChEMBLiCHEMBL323.
GuidetoPHARMACOLOGYi7.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSitePlusiP30994.

Proteomic databases

PaxDbiP30994.
PRIDEiP30994.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29581.
KEGGirno:29581.
UCSCiRGD:61801. rat.

Organism-specific databases

CTDi3357.
RGDi61801. Htr2b.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000240378.
HOVERGENiHBG107487.
InParanoidiP30994.
KOiK04157.
PhylomeDBiP30994.

Miscellaneous databases

PROiP30994.

Family and domain databases

InterProiIPR000482. 5HT2B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF31. PTHR24247:SF31. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00651. 5HT2BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei5HT2B_RAT
AccessioniPrimary (citable) accession number: P30994
Secondary accession number(s): Q9QW44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.