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P30994

- 5HT2B_RAT

UniProt

P30994 - 5HT2B_RAT

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Protein

5-hydroxytryptamine receptor 2B

Gene

Htr2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. Plays a role in the regulation of behavior, including impulsive behavior. Required for normal proliferation of embryonic cardiac myocytes and normal heart development. Protects cardiomyocytes against apoptosis. Plays a role in the adaptation of pulmonary arteries to chronic hypoxia. Plays a role in vasoconstriction. Required for normal osteoblast function and proliferation, and for maintaining normal bone density. Required for normal proliferation of the interstitial cells of Cajal in the intestine (By similarity). May play a role in the perception of pain.By similarity2 Publications

GO - Molecular functioni

  1. drug binding Source: UniProtKB
  2. G-protein alpha-subunit binding Source: UniProtKB
  3. Ras GTPase activator activity Source: UniProtKB
  4. serotonin binding Source: UniProtKB
  5. serotonin receptor activity Source: UniProtKB

GO - Biological processi

  1. behavior Source: UniProtKB-KW
  2. calcium-mediated signaling Source: UniProtKB
  3. cardiac muscle hypertrophy Source: UniProtKB
  4. cellular response to temperature stimulus Source: UniProtKB
  5. cGMP biosynthetic process Source: UniProtKB
  6. embryonic morphogenesis Source: UniProtKB
  7. ERK1 and ERK2 cascade Source: UniProtKB
  8. G-protein coupled receptor signaling pathway Source: UniProtKB
  9. heart morphogenesis Source: UniProtKB
  10. intestine smooth muscle contraction Source: UniProtKB
  11. negative regulation of apoptotic process Source: UniProtKB
  12. negative regulation of autophagy Source: UniProtKB
  13. negative regulation of cell death Source: UniProtKB
  14. neural crest cell differentiation Source: UniProtKB
  15. neural crest cell migration Source: UniProtKB
  16. phosphatidylinositol 3-kinase signaling Source: UniProtKB
  17. phosphorylation Source: UniProtKB
  18. positive regulation of cell division Source: UniProtKB
  19. positive regulation of cell proliferation Source: UniProtKB
  20. positive regulation of cytokine production Source: UniProtKB
  21. positive regulation of cytokine secretion Source: UniProtKB
  22. positive regulation of endothelial cell proliferation Source: UniProtKB
  23. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  24. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  25. positive regulation of nitric-oxide synthase activity Source: UniProtKB
  26. positive regulation of phosphatidylinositol biosynthetic process Source: UniProtKB
  27. positive regulation of Ras GTPase activity Source: GOC
  28. protein kinase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
  29. protein kinase C signaling Source: UniProtKB
  30. regulation of behavior Source: UniProtKB
  31. release of sequestered calcium ion into cytosol Source: UniProtKB
  32. response to drug Source: UniProtKB
  33. serotonin receptor signaling pathway Source: UniProtKB
  34. vasoconstriction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 2B
Short name:
5-HT-2B
Short name:
5-HT2B
Alternative name(s):
5-HT-2F
Serotonin receptor 2B
Stomach fundus serotonin receptor
Gene namesi
Name:Htr2b
Synonyms:Srl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi61801. Htr2b.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell junctionsynapsesynaptosome By similarity

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cytoplasm Source: UniProtKB
  3. integral component of plasma membrane Source: InterPro
  4. neuron projection Source: UniProtKB-KW
  5. plasma membrane Source: UniProtKB
  6. synapse Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4794795-hydroxytryptamine receptor 2BPRO_0000068956Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi127 ↔ 206PROSITE-ProRule annotation
Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi349 ↔ 352PROSITE-ProRule annotation
Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence Analysis
Lipidationi396 – 3961S-palmitoyl cysteineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PRIDEiP30994.

PTM databases

PhosphoSiteiP30994.

Expressioni

Tissue specificityi

Stomach fundus.1 Publication

Gene expression databases

GenevestigatoriP30994.

Interactioni

Subunit structurei

Interacts with MPDZ.By similarity

Protein-protein interaction databases

BioGridi248214. 1 interaction.
IntActiP30994. 1 interaction.
MINTiMINT-4000039.
STRINGi10116.ENSRNOP00000023829.

Structurei

3D structure databases

ProteinModelPortaliP30994.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5555ExtracellularBy similarityAdd
BLAST
Topological domaini79 – 8911CytoplasmicBy similarityAdd
BLAST
Topological domaini113 – 12816ExtracellularBy similarityAdd
BLAST
Topological domaini151 – 17020CytoplasmicBy similarityAdd
BLAST
Topological domaini192 – 21524ExtracellularBy similarityAdd
BLAST
Topological domaini239 – 32385CytoplasmicBy similarityAdd
BLAST
Topological domaini345 – 35915ExtracellularBy similarityAdd
BLAST
Topological domaini382 – 47998CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei56 – 7823Helical; Name=1By similarityAdd
BLAST
Transmembranei90 – 11223Helical; Name=2By similarityAdd
BLAST
Transmembranei129 – 15022Helical; Name=3By similarityAdd
BLAST
Transmembranei171 – 19121Helical; Name=4By similarityAdd
BLAST
Transmembranei216 – 23823Helical; Name=5By similarityAdd
BLAST
Transmembranei324 – 34421Helical; Name=6By similarityAdd
BLAST
Transmembranei360 – 38122Helical; Name=7By similarityAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 1396Agonist bindingBy similarity
Regioni336 – 3405Agonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi151 – 1533DRY motif; important for ligand-induced conformation changesBy similarity
Motifi211 – 2144[DE]RFG motif; may stabilize a conformation that preferentially activates signaling via beta-arrestin family membersBy similarity
Motifi375 – 3795NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity
Motifi477 – 4793PDZ-binding

Domaini

Ligands are bound in a hydrophobic pocket formed by the transmembrane helices.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG247243.
HOGENOMiHOG000240378.
HOVERGENiHBG107487.
InParanoidiP30994.
KOiK04157.
PhylomeDBiP30994.

Family and domain databases

Gene3Di1.20.1070.10. 2 hits.
InterProiIPR000482. 5HT2B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF31. PTHR24247:SF31. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00651. 5HT2BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30994-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASSYKMSEQ STISEHILQK TCDHLILTDR SGLKAESAAE EMKQTAENQG
60 70 80 90 100
NTVHWAALLI FAVIIPTIGG NILVILAVSL EKRLQYATNY FLMSLAVADL
110 120 130 140 150
LVGLFVMPIA LLTIMFEATW PLPLALCPAW LFLDVLFSTA SIMHLCAISL
160 170 180 190 200
DRYIAIKKPI QANQCNSRTT AFVKITVVWL ISIGIAIPVP IKGIEADVVN
210 220 230 240 250
AHNITCELTK DRFGSFMLFG SLAAFFAPLT IMIVTYFLTI HALRKKAYLV
260 270 280 290 300
RNRPPQRLTR WTVSTVLQRE DSSFSSPEKM VMLDGSHKDK ILPNSTDETL
310 320 330 340 350
MRRMSSAGKK PAQTISNEQR ASKVLGIVFL FFLLMWCPFF ITNVTLALCD
360 370 380 390 400
SCNQTTLKTL LQIFVWVGYV SSGVNPLIYT LFNKTFREAF GRYITCNYQA
410 420 430 440 450
TKSVKVLRKC SSTLYFGNSM VENSKFFTKH GIRNGINPAM YQSPVRLRSS
460 470
TIQSSSIILL NTFLTENDGD KVEDQVSYI
Length:479
Mass (Da):53,652
Last modified:July 1, 1993 - v1
Checksum:i17FFC73213B42038
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti281 – 2811V → A(PubMed:1331748)Curated
Sequence conflicti296 – 2961T → I no nucleotide entry (PubMed:1331748)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66842 mRNA. Translation: CAA47318.1.
PIRiS23562.
RefSeqiNP_058946.1. NM_017250.1.
UniGeneiRn.10425.

Genome annotation databases

GeneIDi29581.
KEGGirno:29581.
UCSCiRGD:61801. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66842 mRNA. Translation: CAA47318.1 .
PIRi S23562.
RefSeqi NP_058946.1. NM_017250.1.
UniGenei Rn.10425.

3D structure databases

ProteinModelPortali P30994.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248214. 1 interaction.
IntActi P30994. 1 interaction.
MINTi MINT-4000039.
STRINGi 10116.ENSRNOP00000023829.

Chemistry

BindingDBi P30994.
ChEMBLi CHEMBL2094123.
GuidetoPHARMACOLOGYi 7.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P30994.

Proteomic databases

PRIDEi P30994.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 29581.
KEGGi rno:29581.
UCSCi RGD:61801. rat.

Organism-specific databases

CTDi 3357.
RGDi 61801. Htr2b.

Phylogenomic databases

eggNOGi NOG247243.
HOGENOMi HOG000240378.
HOVERGENi HBG107487.
InParanoidi P30994.
KOi K04157.
PhylomeDBi P30994.

Miscellaneous databases

NextBioi 609690.

Gene expression databases

Genevestigatori P30994.

Family and domain databases

Gene3Di 1.20.1070.10. 2 hits.
InterProi IPR000482. 5HT2B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view ]
PANTHERi PTHR24247:SF31. PTHR24247:SF31. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00651. 5HT2BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and functional characterization of the rat stomach fundus serotonin receptor."
    Foguet M., Hoyer D., Pardo L.A., Parekh A., Kluxen F.-W., Kalkman M.O., Stuehmer W., Luebbert H.
    EMBO J. 11:3481-3487(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
  2. "Molecular cloning, functional expression, and pharmacological characterization of a novel serotonin receptor (5-hydroxytryptamine2F) from rat stomach fundus."
    Kursar J.D., Nelson D.L., Wainscott D.B., Cohen M.L., Baez M.
    Mol. Pharmacol. 42:549-557(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Gastric fundus.
  3. "Antinociceptive effect of peripheral serotonin 5-HT2B receptor activation on neuropathic pain."
    Urtikova N., Berson N., Van Steenwinckel J., Doly S., Truchetto J., Maroteaux L., Pohl M., Conrath M.
    Pain 153:1320-1331(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry namei5HT2B_RAT
AccessioniPrimary (citable) accession number: P30994
Secondary accession number(s): Q9QW44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3