Skip Header

Contribute Send feedback
Read comments (?) or add your own

P30994 (5HT2B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-hydroxytryptamine receptor 2B
Short name=5-HT-2B
Short name=5-HT2B
Alternative name(s):
5-HT-2F
Serotonin receptor 2B
Stomach fundus serotonin receptor
Gene names
Name:Htr2b
Synonyms:Srl
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subunit structure

Interacts with MPDZ By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Stomach fundus. Ref.1

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processERK1 and ERK2 cascade

Inferred from sequence or structural similarity. Source: UniProtKB

G-protein coupled receptor internalization

Inferred from sequence or structural similarity. Source: UniProtKB

behavior

Inferred from electronic annotation. Source: InterPro

cGMP biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

calcium-mediated signaling

Inferred from sequence or structural similarity. Source: UniProtKB

cardiac muscle hypertrophy

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to calcium ion

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to temperature stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

embryonic morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

heart morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

intestine smooth muscle contraction

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of autophagy

Inferred from sequence or structural similarity. Source: UniProtKB

neural crest cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

phosphatidylinositol 3-kinase cascade

Inferred from sequence or structural similarity. Source: UniProtKB

phosphatidylinositol biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of I-kappaB kinase/NF-kappaB cascade

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cell division

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cytokine production

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cytokine secretion

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of diacylglycerol biosynthetic process

Inferred from electronic annotation. Source: InterPro

positive regulation of endothelial cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of inositol trisphosphate biosynthetic process

Inferred from electronic annotation. Source: InterPro

positive regulation of nitric-oxide synthase activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein kinase C signaling cascade

Inferred from sequence or structural similarity. Source: UniProtKB

protein kinase C-activating G-protein coupled receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of behavior

Inferred from sequence or structural similarity. Source: UniProtKB

release of sequestered calcium ion into cytosol

Inferred from sequence or structural similarity. Source: UniProtKB

response to drug

Inferred from sequence or structural similarity. Source: UniProtKB

vasoconstriction

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

integral to plasma membrane

Inferred from electronic annotation. Source: InterPro

plasma membrane

Inferred from direct assay PubMed 19023134. Source: UniProtKB

   Molecular_functionG-protein alpha-subunit binding

Inferred from sequence or structural similarity. Source: UniProtKB

Ras GTPase activator activity

Inferred from sequence or structural similarity. Source: UniProtKB

calcium channel activity

Inferred from sequence or structural similarity. Source: UniProtKB

drug binding

Inferred from sequence or structural similarity. Source: UniProtKB

phosphatidylinositol phospholipase C activity

Inferred from sequence or structural similarity. Source: UniProtKB

serotonin binding

Inferred from sequence or structural similarity. Source: UniProtKB

serotonin receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4794795-hydroxytryptamine receptor 2B
PRO_0000068956

Regions

Topological domain1 – 5555Extracellular By similarity
Transmembrane56 – 7823Helical; Name=1; By similarity
Topological domain79 – 9012Cytoplasmic By similarity
Transmembrane91 – 11222Helical; Name=2; By similarity
Topological domain113 – 12816Extracellular By similarity
Transmembrane129 – 15022Helical; Name=3; By similarity
Topological domain151 – 17020Cytoplasmic By similarity
Transmembrane171 – 19121Helical; Name=4; By similarity
Topological domain192 – 21524Extracellular By similarity
Transmembrane216 – 23823Helical; Name=5; By similarity
Topological domain239 – 32385Cytoplasmic By similarity
Transmembrane324 – 34421Helical; Name=6; By similarity
Topological domain345 – 35915Extracellular By similarity
Transmembrane360 – 38223Helical; Name=7; By similarity
Topological domain383 – 47997Cytoplasmic By similarity
Motif477 – 4793PDZ-binding

Amino acid modifications

Lipidation3961S-palmitoyl cysteine Potential
Glycosylation2031N-linked (GlcNAc...) Potential
Glycosylation3531N-linked (GlcNAc...) Potential
Disulfide bond127 ↔ 206 By similarity

Experimental info

Sequence conflict2811V → A Ref.2
Sequence conflict2961T → I Ref.2

Sequences

Sequence LengthMass (Da)Tools
P30994 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 17FFC73213B42038

FASTA47953,652
        10         20         30         40         50         60 
MASSYKMSEQ STISEHILQK TCDHLILTDR SGLKAESAAE EMKQTAENQG NTVHWAALLI 

        70         80         90        100        110        120 
FAVIIPTIGG NILVILAVSL EKRLQYATNY FLMSLAVADL LVGLFVMPIA LLTIMFEATW 

       130        140        150        160        170        180 
PLPLALCPAW LFLDVLFSTA SIMHLCAISL DRYIAIKKPI QANQCNSRTT AFVKITVVWL 

       190        200        210        220        230        240 
ISIGIAIPVP IKGIEADVVN AHNITCELTK DRFGSFMLFG SLAAFFAPLT IMIVTYFLTI 

       250        260        270        280        290        300 
HALRKKAYLV RNRPPQRLTR WTVSTVLQRE DSSFSSPEKM VMLDGSHKDK ILPNSTDETL 

       310        320        330        340        350        360 
MRRMSSAGKK PAQTISNEQR ASKVLGIVFL FFLLMWCPFF ITNVTLALCD SCNQTTLKTL 

       370        380        390        400        410        420 
LQIFVWVGYV SSGVNPLIYT LFNKTFREAF GRYITCNYQA TKSVKVLRKC SSTLYFGNSM 

       430        440        450        460        470 
VENSKFFTKH GIRNGINPAM YQSPVRLRSS TIQSSSIILL NTFLTENDGD KVEDQVSYI

« Hide

References

[1]"Cloning and functional characterization of the rat stomach fundus serotonin receptor."
Foguet M., Hoyer D., Pardo L.A., Parekh A., Kluxen F.-W., Kalkman M.O., Stuehmer W., Luebbert H.
EMBO J. 11:3481-3487(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
[2]"Molecular cloning, functional expression, and pharmacological characterization of a novel serotonin receptor (5-hydroxytryptamine2F) from rat stomach fundus."
Kursar J.D., Nelson D.L., Wainscott D.B., Cohen M.L., Baez M.
Mol. Pharmacol. 42:549-557(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Gastric fundus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X66842 mRNA. Translation: CAA47318.1.
IPIIPI00213820.
PIRS23562.
RefSeqNP_058946.1. NM_017250.1.
UniGeneRn.10425.

3D structure databases

ProteinModelPortalP30994.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-4000039.
STRING10116.ENSRNOP00000023829.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP30994.

Proteomic databases

PRIDEP30994.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID29581.
KEGGrno:29581.
UCSCRGD:61801. rat.

Organism-specific databases

CTD3357.
RGD61801. Htr2b.

Phylogenomic databases

eggNOGNOG247243.
HOGENOMHOG000240378.
HOVERGENHBG107487.
InParanoidP30994.
KOK04157.
OrthoDBEOG41ZF9R.

Gene expression databases

ArrayExpressP30994.
GenevestigatorP30994.
GermOnlineENSRNOG00000017625. Rattus norvegicus.

Family and domain databases

InterProIPR000482. 5HT2B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24247. PTHR24247. 1 hit.
PTHR24247:SF31. PTHR24247:SF31. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00651. 5HT2BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP30994.
ChEMBLCHEMBL323.
NextBio609690.

Entry information

Entry name5HT2B_RAT
AccessionPrimary (citable) accession number: P30994
Secondary accession number(s): Q9QW44
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 29, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents