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Protein

C5a anaphylatoxin chemotactic receptor 1

Gene

C5ar1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.By similarity

GO - Molecular functioni

GO - Biological processi

  • animal organ regeneration Source: Ensembl
  • apoptotic process Source: Ensembl
  • cell proliferation in hindbrain Source: Ensembl
  • defense response to Gram-positive bacterium Source: MGI
  • inflammatory response Source: GO_Central
  • mRNA transcription from RNA polymerase II promoter Source: UniProtKB
  • negative regulation of neuron apoptotic process Source: Ensembl
  • neutrophil chemotaxis Source: MGI
  • phospholipase C-activating G-protein coupled receptor signaling pathway Source: GO_Central
  • positive regulation of angiogenesis Source: BHF-UCL
  • positive regulation of cytosolic calcium ion concentration Source: GO_Central
  • positive regulation of epithelial cell proliferation Source: UniProtKB
  • positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • positive regulation of macrophage chemotaxis Source: BHF-UCL
  • positive regulation of neutrophil chemotaxis Source: BHF-UCL
  • positive regulation of vascular endothelial growth factor production Source: BHF-UCL
  • response to lipopolysaccharide Source: Ensembl
  • response to peptidoglycan Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-418594. G alpha (i) signalling events.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-977606. Regulation of Complement cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
C5a anaphylatoxin chemotactic receptor 1
Alternative name(s):
C5a anaphylatoxin chemotactic receptor
Short name:
C5a-R
Short name:
C5aR
CD_antigen: CD88
Gene namesi
Name:C5ar1
Synonyms:C5ar, C5r1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:88232. C5ar1.

Subcellular locationi

  • Cell membrane; Multi-pass membrane protein By similarity
  • Cytoplasmic vesicle By similarity

  • Note: Phosphorylated C5aR colocalizes with ARRB1 and ARRB2 in cytoplasmic vesicles.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 40ExtracellularSequence analysisAdd BLAST40
Transmembranei41 – 61Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini62 – 72CytoplasmicSequence analysisAdd BLAST11
Transmembranei73 – 93Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini94 – 109ExtracellularSequence analysisAdd BLAST16
Transmembranei110 – 130Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini131 – 152CytoplasmicSequence analysisAdd BLAST22
Transmembranei153 – 173Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini174 – 207ExtracellularSequence analysisAdd BLAST34
Transmembranei208 – 228Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini229 – 247CytoplasmicSequence analysisAdd BLAST19
Transmembranei248 – 268Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini269 – 284ExtracellularSequence analysisAdd BLAST16
Transmembranei285 – 305Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini306 – 351CytoplasmicSequence analysisAdd BLAST46

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5746.
GuidetoPHARMACOLOGYi32.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000692111 – 351C5a anaphylatoxin chemotactic receptor 1CuratedAdd BLAST351

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi6N-linked (GlcNAc...)Sequence analysis1
Modified residuei13SulfotyrosineBy similarity1
Modified residuei16SulfotyrosineBy similarity1
Disulfide bondi109 ↔ 188PROSITE-ProRule annotation
Modified residuei318PhosphoserineBy similarity1
Modified residuei325PhosphoserineCombined sources1
Modified residuei328PhosphoserineBy similarity1
Modified residuei333PhosphoserineCombined sources1
Modified residuei339PhosphoserineBy similarity1

Post-translational modificationi

Sulfation plays a critical role in the association of C5aR with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.By similarity
Phosphorylated on serine residues in response to C5a binding, resulting in internalization of the receptor and short-term desensitization to C5a.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

Proteomic databases

PaxDbiP30993.
PeptideAtlasiP30993.
PRIDEiP30993.

PTM databases

iPTMnetiP30993.
PhosphoSitePlusiP30993.

Expressioni

Gene expression databases

BgeeiENSMUSG00000049130.
CleanExiMM_C5AR1.
GenevisibleiP30993. MM.

Interactioni

Subunit structurei

Homodimer. May also form higher-order oligomers. Interacts (when phosphorylated) with ARRB1 and ARRB2; the interaction is associated with internalization of C5aR.By similarity

Protein-protein interaction databases

IntActiP30993. 1 interactor.
STRINGi10090.ENSMUSP00000060003.

Structurei

3D structure databases

ProteinModelPortaliP30993.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF0K. Eukaryota.
ENOG410Y9HH. LUCA.
GeneTreeiENSGT00760000118990.
HOGENOMiHOG000234122.
HOVERGENiHBG107547.
InParanoidiP30993.
KOiK04010.
OMAiIWCQNFR.
OrthoDBiEOG091G0DT8.

Family and domain databases

InterProiIPR001274. Anaphtx_C5AR1/C5AR2.
IPR002234. Anphylx_rcpt.
IPR000826. Formyl_rcpt-rel.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24225. PTHR24225. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01104. ANPHYLATOXNR.
PR00426. C5ANPHYLTXNR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30993-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPIDNSSFE INYDHYGTMD PNIPADGIHL PKRQPGDVAA LIIYSVVFLV
60 70 80 90 100
GVPGNALVVW VTAFEARRAV NAIWFLNLAV ADLLSCLALP VLFTTVLNHN
110 120 130 140 150
YWYFDATACI VLPSLILLNM YASILLLATI SADRFLLVFK PIWCQKVRGT
160 170 180 190 200
GLAWMACGVA WVLALLLTIP SFVYREAYKD FYSEHTVCGI NYGGGSFPKE
210 220 230 240 250
KAVAILRLMV GFVLPLLTLN ICYTFLLLRT WSRKATRSTK TLKVVMAVVI
260 270 280 290 300
CFFIFWLPYQ VTGVMIAWLP PSSPTLKRVE KLNSLCVSLA YINCCVNPII
310 320 330 340 350
YVMAGQGFHG RLLRSLPSII RNALSEDSVG RDSKTFTPST TDTSTRKSQA

V
Length:351
Mass (Da):39,023
Last modified:January 7, 2015 - v2
Checksum:i1186314DBAECC8F2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66 – 70ARRAV → PDGPS in AAB97774 (PubMed:1401897).Curated5
Sequence conflicti89L → M in AAB97774 (PubMed:1401897).Curated1
Sequence conflicti341T → D in AAB97774 (PubMed:1401897).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S46665, S50577 Genomic DNA. Translation: AAB97774.1.
AY220494 mRNA. Translation: AAP50849.1.
AK158027 mRNA. Translation: BAE34324.1.
AC156630 Genomic DNA. No translation available.
BC125641 mRNA. Translation: AAI25642.1.
BC125643 mRNA. Translation: AAI25644.1.
CCDSiCCDS20846.1.
PIRiA46525.
RefSeqiNP_001167021.1. NM_001173550.1.
NP_031603.2. NM_007577.4.
UniGeneiMm.247623.

Genome annotation databases

EnsembliENSMUST00000050770; ENSMUSP00000060003; ENSMUSG00000049130.
ENSMUST00000168818; ENSMUSP00000129972; ENSMUSG00000049130.
GeneIDi12273.
KEGGimmu:12273.
UCSCiuc009fhl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S46665, S50577 Genomic DNA. Translation: AAB97774.1.
AY220494 mRNA. Translation: AAP50849.1.
AK158027 mRNA. Translation: BAE34324.1.
AC156630 Genomic DNA. No translation available.
BC125641 mRNA. Translation: AAI25642.1.
BC125643 mRNA. Translation: AAI25644.1.
CCDSiCCDS20846.1.
PIRiA46525.
RefSeqiNP_001167021.1. NM_001173550.1.
NP_031603.2. NM_007577.4.
UniGeneiMm.247623.

3D structure databases

ProteinModelPortaliP30993.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP30993. 1 interactor.
STRINGi10090.ENSMUSP00000060003.

Chemistry databases

ChEMBLiCHEMBL5746.
GuidetoPHARMACOLOGYi32.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP30993.
PhosphoSitePlusiP30993.

Proteomic databases

PaxDbiP30993.
PeptideAtlasiP30993.
PRIDEiP30993.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050770; ENSMUSP00000060003; ENSMUSG00000049130.
ENSMUST00000168818; ENSMUSP00000129972; ENSMUSG00000049130.
GeneIDi12273.
KEGGimmu:12273.
UCSCiuc009fhl.2. mouse.

Organism-specific databases

CTDi728.
MGIiMGI:88232. C5ar1.

Phylogenomic databases

eggNOGiENOG410IF0K. Eukaryota.
ENOG410Y9HH. LUCA.
GeneTreeiENSGT00760000118990.
HOGENOMiHOG000234122.
HOVERGENiHBG107547.
InParanoidiP30993.
KOiK04010.
OMAiIWCQNFR.
OrthoDBiEOG091G0DT8.

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-418594. G alpha (i) signalling events.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-977606. Regulation of Complement cascade.

Miscellaneous databases

PROiP30993.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049130.
CleanExiMM_C5AR1.
GenevisibleiP30993. MM.

Family and domain databases

InterProiIPR001274. Anaphtx_C5AR1/C5AR2.
IPR002234. Anphylx_rcpt.
IPR000826. Formyl_rcpt-rel.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24225. PTHR24225. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01104. ANPHYLATOXNR.
PR00426. C5ANPHYLTXNR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC5AR1_MOUSE
AccessioniPrimary (citable) accession number: P30993
Secondary accession number(s): Q3TZ86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 7, 2015
Last modified: November 30, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.