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Protein

C5a anaphylatoxin chemotactic receptor 1

Gene

C5ar1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.By similarity

GO - Molecular functioni

GO - Biological processi

  • complement component C5a signaling pathway Source: MGI
  • defense response to Gram-positive bacterium Source: MGI
  • mRNA transcription from RNA polymerase II promoter Source: UniProtKB
  • neutrophil chemotaxis Source: MGI
  • positive regulation of angiogenesis Source: BHF-UCL
  • positive regulation of epithelial cell proliferation Source: UniProtKB
  • positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • positive regulation of macrophage chemotaxis Source: BHF-UCL
  • positive regulation of neutrophil chemotaxis Source: BHF-UCL
  • positive regulation of vascular endothelial growth factor production Source: BHF-UCL
  • response to peptidoglycan Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

ReactomeiREACT_319210. Peptide ligand-binding receptors.
REACT_331048. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
C5a anaphylatoxin chemotactic receptor 1
Alternative name(s):
C5a anaphylatoxin chemotactic receptor
Short name:
C5a-R
Short name:
C5aR
CD_antigen: CD88
Gene namesi
Name:C5ar1
Synonyms:C5ar, C5r1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:88232. C5ar1.

Subcellular locationi

  • Cell membrane; Multi-pass membrane protein By similarity
  • Cytoplasmic vesicle By similarity

  • Note: Phosphorylated C5aR colocalizes with ARRB1 and ARRB2 in cytoplasmic vesicles.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4040ExtracellularSequence AnalysisAdd
BLAST
Transmembranei41 – 6121Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini62 – 7211CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei73 – 9321Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini94 – 10916ExtracellularSequence AnalysisAdd
BLAST
Transmembranei110 – 13021Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini131 – 15222CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei153 – 17321Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini174 – 20734ExtracellularSequence AnalysisAdd
BLAST
Transmembranei208 – 22821Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini229 – 24719CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei248 – 26821Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini269 – 28416ExtracellularSequence AnalysisAdd
BLAST
Transmembranei285 – 30521Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini306 – 35146CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351C5a anaphylatoxin chemotactic receptor 1CuratedPRO_0000069211Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi6 – 61N-linked (GlcNAc...)Sequence Analysis
Modified residuei13 – 131SulfotyrosineBy similarity
Modified residuei16 – 161SulfotyrosineBy similarity
Disulfide bondi109 ↔ 188PROSITE-ProRule annotation
Modified residuei315 – 3151PhosphoserineBy similarity
Modified residuei318 – 3181PhosphoserineBy similarity
Modified residuei325 – 3251Phosphoserine1 Publication
Modified residuei328 – 3281PhosphoserineBy similarity
Modified residuei333 – 3331Phosphoserine1 Publication
Modified residuei339 – 3391PhosphoserineBy similarity

Post-translational modificationi

Sulfation plays a critical role in the association of C5aR with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.By similarity
Phosphorylated on serine residues in response to C5a binding, resulting in internalization of the receptor and short-term desensitization to C5a.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

Proteomic databases

PaxDbiP30993.
PRIDEiP30993.

PTM databases

PhosphoSiteiP30993.

Expressioni

Gene expression databases

CleanExiMM_C5AR1.
GenevestigatoriP30993.

Interactioni

Subunit structurei

Homodimer. May also form higher-order oligmers. Interacts (when phosphorylated) with ARRB1 and ARRB2; the interaction is associated with internalization of C5aR.By similarity

Protein-protein interaction databases

IntActiP30993. 1 interaction.
STRINGi10090.ENSMUSP00000060003.

Structurei

3D structure databases

ProteinModelPortaliP30993.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG151055.
GeneTreeiENSGT00760000118990.
HOGENOMiHOG000234122.
HOVERGENiHBG107547.
InParanoidiP30993.
KOiK04010.
OMAiYINCCIN.
OrthoDBiEOG71P2BC.

Family and domain databases

InterProiIPR001274. Anaphtx_C5AR1/C5AR2.
IPR002234. Anphylx_rcpt.
IPR000826. Formyl_rcpt-rel.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24225. PTHR24225. 1 hit.
PTHR24225:SF28. PTHR24225:SF28. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01104. ANPHYLATOXNR.
PR00426. C5ANPHYLTXNR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30993-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPIDNSSFE INYDHYGTMD PNIPADGIHL PKRQPGDVAA LIIYSVVFLV
60 70 80 90 100
GVPGNALVVW VTAFEARRAV NAIWFLNLAV ADLLSCLALP VLFTTVLNHN
110 120 130 140 150
YWYFDATACI VLPSLILLNM YASILLLATI SADRFLLVFK PIWCQKVRGT
160 170 180 190 200
GLAWMACGVA WVLALLLTIP SFVYREAYKD FYSEHTVCGI NYGGGSFPKE
210 220 230 240 250
KAVAILRLMV GFVLPLLTLN ICYTFLLLRT WSRKATRSTK TLKVVMAVVI
260 270 280 290 300
CFFIFWLPYQ VTGVMIAWLP PSSPTLKRVE KLNSLCVSLA YINCCVNPII
310 320 330 340 350
YVMAGQGFHG RLLRSLPSII RNALSEDSVG RDSKTFTPST TDTSTRKSQA

V
Length:351
Mass (Da):39,023
Last modified:January 7, 2015 - v2
Checksum:i1186314DBAECC8F2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 705ARRAV → PDGPS in AAB97774 (PubMed:1401897).Curated
Sequence conflicti89 – 891L → M in AAB97774 (PubMed:1401897).Curated
Sequence conflicti341 – 3411T → D in AAB97774 (PubMed:1401897).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S46665, S50577 Genomic DNA. Translation: AAB97774.1.
AY220494 mRNA. Translation: AAP50849.1.
AK158027 mRNA. Translation: BAE34324.1.
AC156630 Genomic DNA. No translation available.
BC125641 mRNA. Translation: AAI25642.1.
BC125643 mRNA. Translation: AAI25644.1.
CCDSiCCDS20846.1.
PIRiA46525.
RefSeqiNP_001167021.1. NM_001173550.1.
NP_031603.2. NM_007577.4.
UniGeneiMm.247623.

Genome annotation databases

EnsembliENSMUST00000050770; ENSMUSP00000060003; ENSMUSG00000049130.
ENSMUST00000168818; ENSMUSP00000129972; ENSMUSG00000049130.
GeneIDi12273.
KEGGimmu:12273.
UCSCiuc009fhl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S46665, S50577 Genomic DNA. Translation: AAB97774.1.
AY220494 mRNA. Translation: AAP50849.1.
AK158027 mRNA. Translation: BAE34324.1.
AC156630 Genomic DNA. No translation available.
BC125641 mRNA. Translation: AAI25642.1.
BC125643 mRNA. Translation: AAI25644.1.
CCDSiCCDS20846.1.
PIRiA46525.
RefSeqiNP_001167021.1. NM_001173550.1.
NP_031603.2. NM_007577.4.
UniGeneiMm.247623.

3D structure databases

ProteinModelPortaliP30993.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP30993. 1 interaction.
STRINGi10090.ENSMUSP00000060003.

Chemistry

ChEMBLiCHEMBL5746.
GuidetoPHARMACOLOGYi32.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP30993.

Proteomic databases

PaxDbiP30993.
PRIDEiP30993.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050770; ENSMUSP00000060003; ENSMUSG00000049130.
ENSMUST00000168818; ENSMUSP00000129972; ENSMUSG00000049130.
GeneIDi12273.
KEGGimmu:12273.
UCSCiuc009fhl.2. mouse.

Organism-specific databases

CTDi728.
MGIiMGI:88232. C5ar1.

Phylogenomic databases

eggNOGiNOG151055.
GeneTreeiENSGT00760000118990.
HOGENOMiHOG000234122.
HOVERGENiHBG107547.
InParanoidiP30993.
KOiK04010.
OMAiYINCCIN.
OrthoDBiEOG71P2BC.

Enzyme and pathway databases

ReactomeiREACT_319210. Peptide ligand-binding receptors.
REACT_331048. G alpha (i) signalling events.

Miscellaneous databases

NextBioi280732.
PROiP30993.
SOURCEiSearch...

Gene expression databases

CleanExiMM_C5AR1.
GenevestigatoriP30993.

Family and domain databases

InterProiIPR001274. Anaphtx_C5AR1/C5AR2.
IPR002234. Anphylx_rcpt.
IPR000826. Formyl_rcpt-rel.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24225. PTHR24225. 1 hit.
PTHR24225:SF28. PTHR24225:SF28. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01104. ANPHYLATOXNR.
PR00426. C5ANPHYLTXNR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural diversity in the extracellular faces of peptidergic G-protein-coupled receptors. Molecular cloning of the mouse C5a anaphylatoxin receptor."
    Gerard C., Bao L., Orozco O., Pearson M., Kunz D., Gerard N.P.
    J. Immunol. 149:2600-2606(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular characterization of the gerbil C5a receptor and identification of a transmembrane domain V amino acid that is crucial for small molecule antagonist interaction."
    Waters S.M., Brodbeck R.M., Steflik J., Yu J., Baltazar C., Peck A.E., Severance D., Zhang L.Y., Currie K., Chenard B.L., Hutchison A.J., Maynard G., Krause J.E.
    J. Biol. Chem. 280:40617-40623(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6JImported.
    Tissue: LungImported.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6JImported.
    Tissue: Inner earImported.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: BrainImported.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-325 AND SER-333, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiC5AR1_MOUSE
AccessioniPrimary (citable) accession number: P30993
Secondary accession number(s): Q3TZ86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 7, 2015
Last modified: May 27, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.