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P30989

- NTR1_HUMAN

UniProt

P30989 - NTR1_HUMAN

Protein

Neurotensin receptor type 1

Gene

NTSR1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 2 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for the tridecapeptide neurotensin (NTS). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis.3 Publications

    GO - Molecular functioni

    1. G-protein coupled neurotensin receptor activity Source: UniProtKB
    2. G-protein coupled receptor activity Source: ProtInc
    3. protein binding Source: IntAct

    GO - Biological processi

    1. adult locomotory behavior Source: Ensembl
    2. G-protein coupled receptor signaling pathway Source: ProtInc
    3. negative regulation of apoptotic process Source: UniProtKB
    4. neuropeptide signaling pathway Source: UniProtKB
    5. synaptic transmission Source: ProtInc

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_14819. Peptide ligand-binding receptors.
    REACT_18283. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neurotensin receptor type 1
    Short name:
    NT-R-1
    Short name:
    NTR1
    Alternative name(s):
    High-affinity levocabastine-insensitive neurotensin receptor
    NTRH
    Gene namesi
    Name:NTSR1
    Synonyms:NTRR
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:8039. NTSR1.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Membrane raft
    Note: Palmitoylation is required for localization at CAV1-enriched membrane rafts.

    GO - Cellular componenti

    1. endoplasmic reticulum Source: ProtInc
    2. Golgi apparatus Source: ProtInc
    3. integral component of plasma membrane Source: UniProtKB
    4. membrane raft Source: UniProtKB
    5. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi381 – 3811C → S: Abolishes palmitoylation; when associated with S-383. 1 Publication
    Mutagenesisi383 – 3831C → S: Abolishes palmitoylation; when associated with S-381. 1 Publication

    Organism-specific databases

    PharmGKBiPA31821.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 418418Neurotensin receptor type 1PRO_0000069946Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi4 – 41N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi141 ↔ 224PROSITE-ProRule annotation
    Lipidationi381 – 3811S-palmitoyl cysteine1 Publication
    Lipidationi383 – 3831S-palmitoyl cysteine1 Publication

    Post-translational modificationi

    N-gycosylated.
    Palmitoylated; this is required for normal localization at membrane rafts and normal GNA11-mediated activation of down-stream signaling cascades. The palmitoylation level increases in response to neurotensin treatment.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    MaxQBiP30989.
    PaxDbiP30989.
    PeptideAtlasiP30989.
    PRIDEiP30989.

    PTM databases

    PhosphoSiteiP30989.

    Expressioni

    Tissue specificityi

    Expressed in prostate (at protein level). Detected in colon and peripheral blood mononuclear cells. Detected at very low levels in brain.2 Publications

    Gene expression databases

    BgeeiP30989.
    CleanExiHS_NTSR1.
    GenevestigatoriP30989.

    Organism-specific databases

    HPAiHPA050299.

    Interactioni

    Subunit structurei

    Interacts (palmitoylated form) with GNA11.2 Publications

    Protein-protein interaction databases

    BioGridi110977. 2 interactions.
    DIPiDIP-60977N.
    IntActiP30989. 1 interaction.
    STRINGi9606.ENSP00000359532.

    Structurei

    Secondary structure

    1
    418
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi326 – 3283
    Beta strandi338 – 3414

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LYWNMR-A321-344[»]
    ProteinModelPortaliP30989.
    SMRiP30989. Positions 48-381.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6767ExtracellularBy similarityAdd
    BLAST
    Topological domaini89 – 10214CytoplasmicBy similarityAdd
    BLAST
    Topological domaini123 – 14220ExtracellularBy similarityAdd
    BLAST
    Topological domaini165 – 18723CytoplasmicBy similarityAdd
    BLAST
    Topological domaini210 – 23425ExtracellularBy similarityAdd
    BLAST
    Topological domaini260 – 30344CytoplasmicBy similarityAdd
    BLAST
    Topological domaini326 – 34318ExtracellularBy similarityAdd
    BLAST
    Topological domaini365 – 41854CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei68 – 8821Helical; Name=1By similarityAdd
    BLAST
    Transmembranei103 – 12220Helical; Name=2By similarityAdd
    BLAST
    Transmembranei143 – 16422Helical; Name=3By similarityAdd
    BLAST
    Transmembranei188 – 20922Helical; Name=4By similarityAdd
    BLAST
    Transmembranei235 – 25925Helical; Name=5By similarityAdd
    BLAST
    Transmembranei304 – 32522Helical; Name=6By similarityAdd
    BLAST
    Transmembranei344 – 36421Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni321 – 34424Neurotensin bindingBy similarityAdd
    BLAST

    Domaini

    The ligand binding pocket consists mainly of extracellular loops ECL2 and ECL3, as well as transmembranes TM6 and TM7.By similarity

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR1 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG303702.
    HOGENOMiHOG000231271.
    HOVERGENiHBG098285.
    InParanoidiP30989.
    KOiK04211.
    OMAiTVMVRQA.
    OrthoDBiEOG75F4DR.
    PhylomeDBiP30989.
    TreeFamiTF337167.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR003985. NT1_rcpt.
    IPR003984. NT_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR01479. NEUROTENSINR.
    PR01480. NEUROTENSN1R.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30989-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLNSSAPGT PGTPAADPFQ RAQAGLEEAL LAPGFGNASG NASERVLAAP    50
    SSELDVNTDI YSKVLVTAVY LALFVVGTVG NTVTAFTLAR KKSLQSLQST 100
    VHYHLGSLAL SDLLTLLLAM PVELYNFIWV HHPWAFGDAG CRGYYFLRDA 150
    CTYATALNVA SLSVERYLAI CHPFKAKTLM SRSRTKKFIS AIWLASALLA 200
    VPMLFTMGEQ NRSADGQHAG GLVCTPTIHT ATVKVVIQVN TFMSFIFPMV 250
    VISVLNTIIA NKLTVMVRQA AEQGQVCTVG GEHSTFSMAI EPGRVQALRH 300
    GVRVLRAVVI AFVVCWLPYH VRRLMFCYIS DEQWTPFLYD FYHYFYMVTN 350
    ALFYVSSTIN PILYNLVSAN FRHIFLATLA CLCPVWRRRR KRPAFSRKAD 400
    SVSSNHTLSS NATRETLY 418
    Length:418
    Mass (Da):46,259
    Last modified:March 29, 2005 - v2
    Checksum:i471AD1C49D29C2C8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti200 – 2001A → T in CAA49675. (PubMed:8381365)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti72 – 721A → V.
    Corresponds to variant rs11698783 [ dbSNP | Ensembl ].
    VAR_059328
    Natural varianti275 – 2751Q → H.
    Corresponds to variant rs35373650 [ dbSNP | Ensembl ].
    VAR_049424
    Natural varianti304 – 3041V → I.
    Corresponds to variant rs2273075 [ dbSNP | Ensembl ].
    VAR_020071

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70070 mRNA. Translation: CAA49675.1.
    AY429106 mRNA. Translation: AAR07901.1.
    AL035669, AL357033 Genomic DNA. Translation: CAC12747.2.
    AL357033 Genomic DNA. Translation: CAC14923.2.
    CCDSiCCDS13502.1.
    PIRiS29506.
    RefSeqiNP_002522.2. NM_002531.2.
    UniGeneiHs.590869.

    Genome annotation databases

    EnsembliENST00000370501; ENSP00000359532; ENSG00000101188.
    GeneIDi4923.
    KEGGihsa:4923.
    UCSCiuc002ydf.3. human.

    Polymorphism databases

    DMDMi62297312.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Wikipedia

    Neurotensin receptor entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70070 mRNA. Translation: CAA49675.1 .
    AY429106 mRNA. Translation: AAR07901.1 .
    AL035669 , AL357033 Genomic DNA. Translation: CAC12747.2 .
    AL357033 Genomic DNA. Translation: CAC14923.2 .
    CCDSi CCDS13502.1.
    PIRi S29506.
    RefSeqi NP_002522.2. NM_002531.2.
    UniGenei Hs.590869.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LYW NMR - A 321-344 [» ]
    ProteinModelPortali P30989.
    SMRi P30989. Positions 48-381.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110977. 2 interactions.
    DIPi DIP-60977N.
    IntActi P30989. 1 interaction.
    STRINGi 9606.ENSP00000359532.

    Chemistry

    BindingDBi P30989.
    ChEMBLi CHEMBL2111438.
    GuidetoPHARMACOLOGYi 309.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P30989.

    Polymorphism databases

    DMDMi 62297312.

    Proteomic databases

    MaxQBi P30989.
    PaxDbi P30989.
    PeptideAtlasi P30989.
    PRIDEi P30989.

    Protocols and materials databases

    DNASUi 4923.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000370501 ; ENSP00000359532 ; ENSG00000101188 .
    GeneIDi 4923.
    KEGGi hsa:4923.
    UCSCi uc002ydf.3. human.

    Organism-specific databases

    CTDi 4923.
    GeneCardsi GC20P061340.
    HGNCi HGNC:8039. NTSR1.
    HPAi HPA050299.
    MIMi 162651. gene.
    neXtProti NX_P30989.
    PharmGKBi PA31821.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG303702.
    HOGENOMi HOG000231271.
    HOVERGENi HBG098285.
    InParanoidi P30989.
    KOi K04211.
    OMAi TVMVRQA.
    OrthoDBi EOG75F4DR.
    PhylomeDBi P30989.
    TreeFami TF337167.

    Enzyme and pathway databases

    Reactomei REACT_14819. Peptide ligand-binding receptors.
    REACT_18283. G alpha (q) signalling events.

    Miscellaneous databases

    GeneWikii Neurotensin_receptor_1.
    GenomeRNAii 4923.
    NextBioi 18959.
    PROi P30989.
    SOURCEi Search...

    Gene expression databases

    Bgeei P30989.
    CleanExi HS_NTSR1.
    Genevestigatori P30989.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR003985. NT1_rcpt.
    IPR003984. NT_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR01479. NEUROTENSINR.
    PR01480. NEUROTENSN1R.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor."
      Vita N., Laurent P., Lefort S., Chalon P., Dumont X., Kaghad M., Gully D., le Fur G., Ferrara P., Caput D.
      FEBS Lett. 317:139-142(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Kopatz S.A., Aronstam R.S., Sharma S.V.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Altered expression of neurotensin receptors is associated with the differentiation state of prostate cancer."
      Swift S.L., Burns J.E., Maitland N.J.
      Cancer Res. 70:347-356(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    5. "Neurotensin receptor-1 inducible palmitoylation is required for efficient receptor-mediated mitogenic-signaling within structured membrane microdomains."
      Heakal Y., Woll M.P., Fox T., Seaton K., Levenson R., Kester M.
      Cancer Biol. Ther. 12:427-435(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH GNA11, PALMITOYLATION AT CYS-381 AND CYS-383, SUBCELLULAR LOCATION, GLYCOSYLATION, MUTAGENESIS OF CYS-381 AND CYS-383.
    6. "Intermolecular interactions between the neurotensin and the third extracellular loop of human neurotensin 1 receptor."
      Da Costa G., Bondon A., Coutant J., Curmi P., Monti J.P.
      J. Biomol. Struct. Dyn. 31:1381-1392(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 321-344 IN COMPLEX WITH NTS, FUNCTION.

    Entry informationi

    Entry nameiNTR1_HUMAN
    AccessioniPrimary (citable) accession number: P30989
    Secondary accession number(s): Q9H4H1, Q9H4T5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 140 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3