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P30989

- NTR1_HUMAN

UniProt

P30989 - NTR1_HUMAN

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Protein

Neurotensin receptor type 1

Gene

NTSR1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for the tridecapeptide neurotensin (NTS). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis.3 Publications

GO - Molecular functioni

  1. G-protein coupled neurotensin receptor activity Source: UniProtKB
  2. G-protein coupled receptor activity Source: ProtInc

GO - Biological processi

  1. adult locomotory behavior Source: Ensembl
  2. G-protein coupled receptor signaling pathway Source: ProtInc
  3. negative regulation of apoptotic process Source: UniProtKB
  4. neuropeptide signaling pathway Source: UniProtKB
  5. synaptic transmission Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_14819. Peptide ligand-binding receptors.
REACT_18283. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurotensin receptor type 1
Short name:
NT-R-1
Short name:
NTR1
Alternative name(s):
High-affinity levocabastine-insensitive neurotensin receptor
NTRH
Gene namesi
Name:NTSR1
Synonyms:NTRR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:8039. NTSR1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Membrane raft
Note: Palmitoylation is required for localization at CAV1-enriched membrane rafts.

GO - Cellular componenti

  1. endoplasmic reticulum Source: ProtInc
  2. Golgi apparatus Source: ProtInc
  3. integral component of plasma membrane Source: UniProtKB
  4. membrane raft Source: UniProtKB
  5. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi381 – 3811C → S: Abolishes palmitoylation; when associated with S-383. 1 Publication
Mutagenesisi383 – 3831C → S: Abolishes palmitoylation; when associated with S-381. 1 Publication

Organism-specific databases

PharmGKBiPA31821.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418Neurotensin receptor type 1PRO_0000069946Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi4 – 41N-linked (GlcNAc...)Sequence Analysis
Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi141 ↔ 224PROSITE-ProRule annotation
Lipidationi381 – 3811S-palmitoyl cysteine1 Publication
Lipidationi383 – 3831S-palmitoyl cysteine1 Publication

Post-translational modificationi

N-gycosylated.
Palmitoylated; this is required for normal localization at membrane rafts and normal GNA11-mediated activation of down-stream signaling cascades. The palmitoylation level increases in response to neurotensin treatment.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP30989.
PaxDbiP30989.
PeptideAtlasiP30989.
PRIDEiP30989.

PTM databases

PhosphoSiteiP30989.

Expressioni

Tissue specificityi

Expressed in prostate (at protein level). Detected in colon and peripheral blood mononuclear cells. Detected at very low levels in brain.2 Publications

Gene expression databases

BgeeiP30989.
CleanExiHS_NTSR1.
GenevestigatoriP30989.

Organism-specific databases

HPAiHPA050299.

Interactioni

Subunit structurei

Interacts (palmitoylated form) with GNA11.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NTSP309902EBI-6655774,EBI-6655817

Protein-protein interaction databases

BioGridi110977. 2 interactions.
DIPiDIP-60977N.
IntActiP30989. 1 interaction.
STRINGi9606.ENSP00000359532.

Structurei

Secondary structure

1
418
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi326 – 3283
Beta strandi338 – 3414

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LYWNMR-A321-344[»]
ProteinModelPortaliP30989.
SMRiP30989. Positions 65-381.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6767ExtracellularBy similarityAdd
BLAST
Topological domaini89 – 10214CytoplasmicBy similarityAdd
BLAST
Topological domaini123 – 14220ExtracellularBy similarityAdd
BLAST
Topological domaini165 – 18723CytoplasmicBy similarityAdd
BLAST
Topological domaini210 – 23425ExtracellularBy similarityAdd
BLAST
Topological domaini260 – 30344CytoplasmicBy similarityAdd
BLAST
Topological domaini326 – 34318ExtracellularBy similarityAdd
BLAST
Topological domaini365 – 41854CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei68 – 8821Helical; Name=1By similarityAdd
BLAST
Transmembranei103 – 12220Helical; Name=2By similarityAdd
BLAST
Transmembranei143 – 16422Helical; Name=3By similarityAdd
BLAST
Transmembranei188 – 20922Helical; Name=4By similarityAdd
BLAST
Transmembranei235 – 25925Helical; Name=5By similarityAdd
BLAST
Transmembranei304 – 32522Helical; Name=6By similarityAdd
BLAST
Transmembranei344 – 36421Helical; Name=7By similarityAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni321 – 34424Neurotensin bindingBy similarityAdd
BLAST

Domaini

The ligand binding pocket consists mainly of extracellular loops ECL2 and ECL3, as well as transmembranes TM6 and TM7.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR1 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG303702.
GeneTreeiENSGT00700000104070.
HOGENOMiHOG000231271.
HOVERGENiHBG098285.
InParanoidiP30989.
KOiK04211.
OMAiTVMVRQA.
OrthoDBiEOG75F4DR.
PhylomeDBiP30989.
TreeFamiTF337167.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003985. NT1_rcpt.
IPR003984. NT_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01479. NEUROTENSINR.
PR01480. NEUROTENSN1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30989-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLNSSAPGT PGTPAADPFQ RAQAGLEEAL LAPGFGNASG NASERVLAAP
60 70 80 90 100
SSELDVNTDI YSKVLVTAVY LALFVVGTVG NTVTAFTLAR KKSLQSLQST
110 120 130 140 150
VHYHLGSLAL SDLLTLLLAM PVELYNFIWV HHPWAFGDAG CRGYYFLRDA
160 170 180 190 200
CTYATALNVA SLSVERYLAI CHPFKAKTLM SRSRTKKFIS AIWLASALLA
210 220 230 240 250
VPMLFTMGEQ NRSADGQHAG GLVCTPTIHT ATVKVVIQVN TFMSFIFPMV
260 270 280 290 300
VISVLNTIIA NKLTVMVRQA AEQGQVCTVG GEHSTFSMAI EPGRVQALRH
310 320 330 340 350
GVRVLRAVVI AFVVCWLPYH VRRLMFCYIS DEQWTPFLYD FYHYFYMVTN
360 370 380 390 400
ALFYVSSTIN PILYNLVSAN FRHIFLATLA CLCPVWRRRR KRPAFSRKAD
410
SVSSNHTLSS NATRETLY
Length:418
Mass (Da):46,259
Last modified:March 29, 2005 - v2
Checksum:i471AD1C49D29C2C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti200 – 2001A → T in CAA49675. (PubMed:8381365)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti72 – 721A → V.
Corresponds to variant rs11698783 [ dbSNP | Ensembl ].
VAR_059328
Natural varianti275 – 2751Q → H.
Corresponds to variant rs35373650 [ dbSNP | Ensembl ].
VAR_049424
Natural varianti304 – 3041V → I.
Corresponds to variant rs2273075 [ dbSNP | Ensembl ].
VAR_020071

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70070 mRNA. Translation: CAA49675.1.
AY429106 mRNA. Translation: AAR07901.1.
AL035669, AL357033 Genomic DNA. Translation: CAC12747.2.
AL357033 Genomic DNA. Translation: CAC14923.2.
CCDSiCCDS13502.1.
PIRiS29506.
RefSeqiNP_002522.2. NM_002531.2.
UniGeneiHs.590869.

Genome annotation databases

EnsembliENST00000370501; ENSP00000359532; ENSG00000101188.
GeneIDi4923.
KEGGihsa:4923.
UCSCiuc002ydf.3. human.

Polymorphism databases

DMDMi62297312.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Neurotensin receptor entry

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70070 mRNA. Translation: CAA49675.1 .
AY429106 mRNA. Translation: AAR07901.1 .
AL035669 , AL357033 Genomic DNA. Translation: CAC12747.2 .
AL357033 Genomic DNA. Translation: CAC14923.2 .
CCDSi CCDS13502.1.
PIRi S29506.
RefSeqi NP_002522.2. NM_002531.2.
UniGenei Hs.590869.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LYW NMR - A 321-344 [» ]
ProteinModelPortali P30989.
SMRi P30989. Positions 65-381.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 110977. 2 interactions.
DIPi DIP-60977N.
IntActi P30989. 1 interaction.
STRINGi 9606.ENSP00000359532.

Chemistry

BindingDBi P30989.
ChEMBLi CHEMBL2111438.
GuidetoPHARMACOLOGYi 309.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P30989.

Polymorphism databases

DMDMi 62297312.

Proteomic databases

MaxQBi P30989.
PaxDbi P30989.
PeptideAtlasi P30989.
PRIDEi P30989.

Protocols and materials databases

DNASUi 4923.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000370501 ; ENSP00000359532 ; ENSG00000101188 .
GeneIDi 4923.
KEGGi hsa:4923.
UCSCi uc002ydf.3. human.

Organism-specific databases

CTDi 4923.
GeneCardsi GC20P061340.
HGNCi HGNC:8039. NTSR1.
HPAi HPA050299.
MIMi 162651. gene.
neXtProti NX_P30989.
PharmGKBi PA31821.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG303702.
GeneTreei ENSGT00700000104070.
HOGENOMi HOG000231271.
HOVERGENi HBG098285.
InParanoidi P30989.
KOi K04211.
OMAi TVMVRQA.
OrthoDBi EOG75F4DR.
PhylomeDBi P30989.
TreeFami TF337167.

Enzyme and pathway databases

Reactomei REACT_14819. Peptide ligand-binding receptors.
REACT_18283. G alpha (q) signalling events.

Miscellaneous databases

GeneWikii Neurotensin_receptor_1.
GenomeRNAii 4923.
NextBioi 18959.
PROi P30989.
SOURCEi Search...

Gene expression databases

Bgeei P30989.
CleanExi HS_NTSR1.
Genevestigatori P30989.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003985. NT1_rcpt.
IPR003984. NT_rcpt.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR01479. NEUROTENSINR.
PR01480. NEUROTENSN1R.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor."
    Vita N., Laurent P., Lefort S., Chalon P., Dumont X., Kaghad M., Gully D., le Fur G., Ferrara P., Caput D.
    FEBS Lett. 317:139-142(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Kopatz S.A., Aronstam R.S., Sharma S.V.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Altered expression of neurotensin receptors is associated with the differentiation state of prostate cancer."
    Swift S.L., Burns J.E., Maitland N.J.
    Cancer Res. 70:347-356(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Neurotensin receptor-1 inducible palmitoylation is required for efficient receptor-mediated mitogenic-signaling within structured membrane microdomains."
    Heakal Y., Woll M.P., Fox T., Seaton K., Levenson R., Kester M.
    Cancer Biol. Ther. 12:427-435(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GNA11, PALMITOYLATION AT CYS-381 AND CYS-383, SUBCELLULAR LOCATION, GLYCOSYLATION, MUTAGENESIS OF CYS-381 AND CYS-383.
  6. "Intermolecular interactions between the neurotensin and the third extracellular loop of human neurotensin 1 receptor."
    Da Costa G., Bondon A., Coutant J., Curmi P., Monti J.P.
    J. Biomol. Struct. Dyn. 31:1381-1392(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 321-344 IN COMPLEX WITH NTS, FUNCTION.

Entry informationi

Entry nameiNTR1_HUMAN
AccessioniPrimary (citable) accession number: P30989
Secondary accession number(s): Q9H4H1, Q9H4T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: March 29, 2005
Last modified: October 29, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3