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Protein

Neurotensin receptor type 1

Gene

NTSR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for the tridecapeptide neurotensin (NTS) (PubMed:8381365, PubMed:21725197, PubMed:23140271). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis (PubMed:21725197).3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-HSA-375276. Peptide ligand-binding receptors.
R-HSA-416476. G alpha (q) signalling events.
SIGNORiP30989.

Protein family/group databases

TCDBi9.A.14.1.14. the g-protein-coupled receptor (gpcr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurotensin receptor type 1
Short name:
NT-R-1
Short name:
NTR1
Alternative name(s):
High-affinity levocabastine-insensitive neurotensin receptor
NTRH
Gene namesi
Name:NTSR1
Synonyms:NTRR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000101188.4.
HGNCiHGNC:8039. NTSR1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 67ExtracellularBy similarityAdd BLAST67
Transmembranei68 – 88Helical; Name=1By similarityAdd BLAST21
Topological domaini89 – 102CytoplasmicBy similarityAdd BLAST14
Transmembranei103 – 122Helical; Name=2By similarityAdd BLAST20
Topological domaini123 – 142ExtracellularBy similarityAdd BLAST20
Transmembranei143 – 164Helical; Name=3By similarityAdd BLAST22
Topological domaini165 – 184CytoplasmicBy similarityAdd BLAST20
Transmembranei185 – 205Helical; Name=4By similarityAdd BLAST21
Topological domaini206 – 234ExtracellularBy similarityAdd BLAST29
Transmembranei235 – 259Helical; Name=5By similarityAdd BLAST25
Topological domaini260 – 303CytoplasmicBy similarityAdd BLAST44
Transmembranei304 – 325Helical; Name=6By similarityAdd BLAST22
Topological domaini326 – 343ExtracellularBy similarityAdd BLAST18
Transmembranei344 – 364Helical; Name=7By similarityAdd BLAST21
Topological domaini365 – 418CytoplasmicBy similarityAdd BLAST54

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi381C → S: Abolishes palmitoylation; when associated with S-383. 1 Publication1
Mutagenesisi383C → S: Abolishes palmitoylation; when associated with S-381. 1 Publication1

Organism-specific databases

DisGeNETi4923.
OpenTargetsiENSG00000101188.
PharmGKBiPA31821.

Chemistry databases

ChEMBLiCHEMBL4123.
GuidetoPHARMACOLOGYi309.

Polymorphism and mutation databases

BioMutaiNTSR1.
DMDMi62297312.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699461 – 418Neurotensin receptor type 1Add BLAST418

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi4N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi37N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi41N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi141 ↔ 224PROSITE-ProRule annotation
Lipidationi381S-palmitoyl cysteine1 Publication1
Lipidationi383S-palmitoyl cysteine1 Publication1

Post-translational modificationi

N-glycosylated.1 Publication
Palmitoylated; this is required for normal localization at membrane rafts and normal GNA11-mediated activation of down-stream signaling cascades. The palmitoylation level increases in response to neurotensin treatment.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP30989.
PaxDbiP30989.
PeptideAtlasiP30989.
PRIDEiP30989.

PTM databases

iPTMnetiP30989.
PhosphoSitePlusiP30989.
SwissPalmiP30989.

Expressioni

Tissue specificityi

Expressed in prostate (at protein level). Detected in colon and peripheral blood mononuclear cells. Detected at very low levels in brain.2 Publications

Gene expression databases

BgeeiENSG00000101188.
CleanExiHS_NTSR1.
GenevisibleiP30989. HS.

Organism-specific databases

HPAiHPA050299.

Interactioni

Subunit structurei

Interacts (palmitoylated form) with GNA11.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NTSP309902EBI-6655774,EBI-6655817

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110977. 1 interactor.
DIPiDIP-60977N.
IntActiP30989. 1 interactor.
STRINGi9606.ENSP00000359532.

Chemistry databases

BindingDBiP30989.

Structurei

Secondary structure

1418
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi326 – 328Combined sources3
Beta strandi338 – 341Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LYWNMR-A321-344[»]
ProteinModelPortaliP30989.
SMRiP30989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni321 – 344Neurotensin bindingBy similarityAdd BLAST24

Domaini

The ligand binding pocket consists mainly of extracellular loops ECL2 and ECL3, as well as transmembrane regions TM6 and TM7.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR1 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00700000104070.
HOGENOMiHOG000231271.
HOVERGENiHBG098285.
InParanoidiP30989.
KOiK04211.
OMAiVPMLFTM.
OrthoDBiEOG091G0Q6E.
PhylomeDBiP30989.
TreeFamiTF337167.

Family and domain databases

CDDicd15355. 7tmA_NTSR1. 1 hit.
InterProiView protein in InterPro
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003985. NT1_rcpt.
IPR003984. NT_rcpt.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR00237. GPCRRHODOPSN.
PR01479. NEUROTENSINR.
PR01480. NEUROTENSN1R.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P30989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLNSSAPGT PGTPAADPFQ RAQAGLEEAL LAPGFGNASG NASERVLAAP
60 70 80 90 100
SSELDVNTDI YSKVLVTAVY LALFVVGTVG NTVTAFTLAR KKSLQSLQST
110 120 130 140 150
VHYHLGSLAL SDLLTLLLAM PVELYNFIWV HHPWAFGDAG CRGYYFLRDA
160 170 180 190 200
CTYATALNVA SLSVERYLAI CHPFKAKTLM SRSRTKKFIS AIWLASALLA
210 220 230 240 250
VPMLFTMGEQ NRSADGQHAG GLVCTPTIHT ATVKVVIQVN TFMSFIFPMV
260 270 280 290 300
VISVLNTIIA NKLTVMVRQA AEQGQVCTVG GEHSTFSMAI EPGRVQALRH
310 320 330 340 350
GVRVLRAVVI AFVVCWLPYH VRRLMFCYIS DEQWTPFLYD FYHYFYMVTN
360 370 380 390 400
ALFYVSSTIN PILYNLVSAN FRHIFLATLA CLCPVWRRRR KRPAFSRKAD
410
SVSSNHTLSS NATRETLY
Length:418
Mass (Da):46,259
Last modified:March 29, 2005 - v2
Checksum:i471AD1C49D29C2C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200A → T in CAA49675 (PubMed:8381365).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05932872A → V. Corresponds to variant dbSNP:rs11698783Ensembl.1
Natural variantiVAR_049424275Q → H. Corresponds to variant dbSNP:rs35373650Ensembl.1
Natural variantiVAR_020071304V → I. Corresponds to variant dbSNP:rs2273075Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70070 mRNA. Translation: CAA49675.1.
AY429106 mRNA. Translation: AAR07901.1.
AL035669, AL357033 Genomic DNA. Translation: CAC12747.2.
AL357033 Genomic DNA. Translation: CAC14923.2.
CCDSiCCDS13502.1.
PIRiS29506.
RefSeqiNP_002522.2. NM_002531.2.
UniGeneiHs.590869.

Genome annotation databases

EnsembliENST00000370501; ENSP00000359532; ENSG00000101188.
GeneIDi4923.
KEGGihsa:4923.
UCSCiuc002ydf.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNTR1_HUMAN
AccessioniPrimary (citable) accession number: P30989
Secondary accession number(s): Q9H4H1, Q9H4T5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: March 29, 2005
Last modified: September 27, 2017
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families