ID   5HT5A_MOUSE             Reviewed;         357 AA.
AC   P30966; Q3URB0;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   24-JAN-2024, entry version 169.
DE   RecName: Full=5-hydroxytryptamine receptor 5A;
DE            Short=5-HT-5;
DE            Short=5-HT-5A;
DE            Short=5-HT5A;
DE   AltName: Full=Serotonin receptor 5A;
GN   Name=Htr5a; Synonyms=5ht5a;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Brain;
RX   PubMed=1464308; DOI=10.1002/j.1460-2075.1992.tb05583.x;
RA   Plassat J.-L., Boschert U., Amlaiky N., Hen R.;
RT   "The mouse 5HT5 receptor reveals a remarkable heterogeneity within the
RT   5HT1D receptor family.";
RL   EMBO J. 11:4779-4786(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Hippocampus;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   CHARACTERIZATION.
RC   TISSUE=Brain;
RX   PubMed=8450829;
RA   Matthes H., Boschert U., Amlaiky N., Grailhe R., Plassat J.-L.,
RA   Muscatelli F., Mattei M.-G., Hen R.;
RT   "Mouse 5-hydroxytryptamine5A and 5-hydroxytryptamine5B receptors define a
RT   new family of serotonin receptors: cloning, functional expression, and
RT   chromosomal localization.";
RL   Mol. Pharmacol. 43:313-319(1993).
CC   -!- FUNCTION: This is one of the several different receptors for 5-
CC       hydroxytryptamine (serotonin), a biogenic hormone that functions as a
CC       neurotransmitter, a hormone, and a mitogen. The activity of this
CC       receptor is mediated by G proteins.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Expressed predominantly in the central nervous
CC       system; in the cerebral cortex, hippocampus, habenula, olfactory bulb
CC       and granular layer of the cerebellum.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR   EMBL; Z18278; CAA79155.1; -; Genomic_DNA.
DR   EMBL; AK141636; BAE24778.1; -; mRNA.
DR   EMBL; CH466524; EDL37224.1; -; Genomic_DNA.
DR   EMBL; BC115911; AAI15912.1; -; mRNA.
DR   EMBL; BC116654; AAI16655.1; -; mRNA.
DR   CCDS; CCDS19141.1; -.
DR   PIR; S28058; S28058.
DR   RefSeq; NP_032340.2; NM_008314.2.
DR   AlphaFoldDB; P30966; -.
DR   SMR; P30966; -.
DR   STRING; 10090.ENSMUSP00000038884; -.
DR   BindingDB; P30966; -.
DR   ChEMBL; CHEMBL3597; -.
DR   DrugCentral; P30966; -.
DR   GuidetoPHARMACOLOGY; 10; -.
DR   GlyCosmos; P30966; 2 sites, No reported glycans.
DR   GlyGen; P30966; 2 sites.
DR   PhosphoSitePlus; P30966; -.
DR   PaxDb; 10090-ENSMUSP00000038884; -.
DR   ProteomicsDB; 296452; -.
DR   Antibodypedia; 18848; 390 antibodies from 30 providers.
DR   DNASU; 15563; -.
DR   Ensembl; ENSMUST00000036227.7; ENSMUSP00000038884.6; ENSMUSG00000039106.7.
DR   GeneID; 15563; -.
DR   KEGG; mmu:15563; -.
DR   UCSC; uc008wtt.1; mouse.
DR   AGR; MGI:96283; -.
DR   CTD; 3361; -.
DR   MGI; MGI:96283; Htr5a.
DR   VEuPathDB; HostDB:ENSMUSG00000039106; -.
DR   eggNOG; KOG3656; Eukaryota.
DR   GeneTree; ENSGT01010000222287; -.
DR   HOGENOM; CLU_009579_11_6_1; -.
DR   InParanoid; P30966; -.
DR   OMA; NLFFRQH; -.
DR   OrthoDB; 2999405at2759; -.
DR   PhylomeDB; P30966; -.
DR   TreeFam; TF316350; -.
DR   Reactome; R-MMU-390666; Serotonin receptors.
DR   Reactome; R-MMU-418594; G alpha (i) signalling events.
DR   BioGRID-ORCS; 15563; 3 hits in 77 CRISPR screens.
DR   PRO; PR:P30966; -.
DR   Proteomes; UP000000589; Chromosome 5.
DR   RNAct; P30966; Protein.
DR   Bgee; ENSMUSG00000039106; Expressed in secondary oocyte and 41 other cell types or tissues.
DR   GO; GO:0030425; C:dendrite; ISO:MGI.
DR   GO; GO:0043204; C:perikaryon; ISO:MGI.
DR   GO; GO:0099634; C:postsynaptic specialization membrane; ISO:MGI.
DR   GO; GO:0004993; F:G protein-coupled serotonin receptor activity; ISO:MGI.
DR   GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR   GO; GO:0051378; F:serotonin binding; IBA:GO_Central.
DR   GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IDA:MGI.
DR   GO; GO:0007198; P:adenylate cyclase-inhibiting serotonin receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR   GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; ISO:MGI.
DR   GO; GO:0021766; P:hippocampus development; IEA:Ensembl.
DR   GO; GO:0032355; P:response to estradiol; IEA:Ensembl.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR001397; 5HT5A_rcpt.
DR   InterPro; IPR002231; 5HT_rcpt.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   PANTHER; PTHR24247; 5-HYDROXYTRYPTAMINE RECEPTOR; 1.
DR   PANTHER; PTHR24247:SF108; 5-HYDROXYTRYPTAMINE RECEPTOR 5A; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00518; 5HT5ARECEPTR.
DR   PRINTS; PR01101; 5HTRECEPTOR.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR   Genevisible; P30966; MM.
PE   1: Evidence at protein level;
KW   Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW   Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..357
FT                   /note="5-hydroxytryptamine receptor 5A"
FT                   /id="PRO_0000068970"
FT   TOPO_DOM        1..40
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        41..63
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        64..78
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        79..99
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        100..115
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        116..137
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        138..158
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        159..181
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        182..198
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        199..219
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        220..282
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        283..303
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        304..320
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        321..341
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        342..357
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        6
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        21
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        120..192
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT   CONFLICT        214..215
FT                   /note="CV -> WL (in Ref. 1; CAA79155)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        261
FT                   /note="V -> A (in Ref. 1; CAA79155)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   357 AA;  40736 MW;  C0F7314A3AB9F75F CRC64;
     MDLPVNLTSF SLSTPSSLEP NRSLDTEVLR PSRPFLSAFR VLVLTLLGFL AAATFTWNLL
     VLATILKVRT FHRVPHNLVA SMAISDVLVA VLVMPLSLVH ELSGRRWQLG RRLCQLWIAC
     DVLCCTASIW NVTAIALDRY WSITRHLEYT LRTRKRVSNV MILLTWALST VISLAPLLFG
     WGETYSEPSE ECQVSREPSY TVFSTVGAFY LPLCVVLFVY WKIYRAAKFR MGSRKTNSVS
     PVPEAVEVKN ATQHPQMVFT VRHATVTFQT EGDTWREQKE QRAALMVGIL IGVFVLCWFP
     FFVTELISPL CSWDVPAIWK SIFLWLGYSN SFFNPLIYTA FNRSYSSAFK VFFSKQQ
//