Reviewed, UniProtKB/Swiss-Prot P30960 (CYCY_BRAJA)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein cycY
Alternative name(s):
    Cytochrome c biogenesis protein cycY

Gene names

Name:	cycY
Synonyms:	ccmG
Ordered Locus Names:	blr0471

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for disulfide bond formation in some periplasmic proteins. Also act as a disulfide oxidoreductase in cytochromes c biogenesis. The cysteines of apocytochromes c must be in the reduced state for covalent linkage between the two moieties to occur By similarity.
Subcellular location	Periplasm Probable.
Sequence similarities	Belongs to the thioredoxin family. DsbE subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis
Cellular component	Periplasm
Domain	Redox-active center Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 37

37

Potential

Chain

38 – 194

157

Thiol:disulfide interchange protein cycY

PRO_0000034287

Regions

Domain

46 – 190

145

Thioredoxin

Amino acid modifications

Disulfide bond

92 ↔ 95

Redox-active Ref.3

Secondary structure

1

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194

Helix Strand Turn

Details...

Helix

75 – 78

4

Beta strand

83 – 88

6

Helix

93 – 105

13

Beta strand

111 – 119

9

Helix

122 – 132

11

Beta strand

137 – 142

6

Helix

146 – 150

5

Beta strand

158 – 162

5

Beta strand

166 – 174

9

Helix

178 – 183

6

Helix

185 – 192

8

Sequences

	Sequence		Length	Mass (Da)
	P30960-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 826A75D22AA0C593	FASTA	194	21,126
	10 20 30 40 50 60 MSEQSTSANP QRRTFLMVLP LIAFIGLALL FWFRLGSGDP SRIPSALIGR PAPQTALPPL 70 80 90 100 110 120 EGLQADNVQV PGLDPAAFKG KVSLVNVWAS WCVPCHDEAP LLTELGKDKR FQLVGINYKD 130 140 150 160 170 180 AADNARRFLG RYGNPFGRVG VDANGRASIE WGVYGVPETF VVGREGTIVY KLVGPITPDN 190 LRSVLLPQME KALK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Discovery and sequence analysis of bacterial genes involved in the biogenesis of c-type cytochromes." Ramseier T.M., Winteler H.V., Hennecke H. J. Biol. Chem. 266:7793-7803(1991) [PubMed: 1850420] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110RIF15.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.
[3]	"Structure of CcmG/DsbE at 1.14 A resolution: high-fidelity reducing activity in an indiscriminately oxidizing environment." Edeling M.A., Guddat L.W., Fabianek R.A., Thoeny-Meyer L., Martin J.L. Structure 10:973-979(2002) [PubMed: 12121652] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.14 ANGSTROMS) OF 39-194, DISULFIDE BOND.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M60874 Genomic DNA. Translation: AAA26196.1. Different initiation.
BA000040 Genomic DNA. Translation: BAC45736.1.

PIR

D39741.

RefSeq

NP_767111.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KNG	X-ray	1.14	A	39-194	[»]

ModBase

Search...

Genome annotation databases

GeneID

1048774.

GenomeReviews

Gene locus blr0471 in contig BA000040_GR.

KEGG

bja:blr0471.

NMPDR

fig|224911.1.peg.471.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P30960.

OMA

P30960. KWLAEFH.

Enzyme and pathway databases

BioCyc

BJAP224911:BLR0471-MON.

Family and domain databases

InterPro

IPR004799. periplasmic_diS_OxRdtase_DsbE.
IPR013740. Redoxin.
IPR017936. Thioredoxin-like.
IPR017937. Thioredoxin_CS.
IPR012335. Thioredoxin_fold.
[Graphical view]

Gene3D

G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

Pfam

PF08534. Redoxin. 1 hit.
[Graphical view]

ProDom

PD003679. Thioredoxin_like. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00385. dsbE. 1 hit.

PROSITE

PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CYCY_BRAJA

Accession

Primary (citable) accession number: P30960

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)