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P30952 (MASY_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase 1, glyoxysomal

EC=2.3.3.9
Gene names
Name:MLS1
Ordered Locus Names:YNL117W
ORF Names:N1921
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This isozyme is necessary for growth on acetate as sole C-source.

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Subunit structure

Homotrimer.

Subcellular location

Glyoxysome.

Sequence similarities

Belongs to the malate synthase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRP40P332032EBI-10428,EBI-701
RSP5P399403EBI-10428,EBI-16219

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554Malate synthase 1, glyoxysomal
PRO_0000166864

Regions

Motif552 – 5543Microbody targeting signal Potential

Sites

Active site1771Proton acceptor By similarity
Active site4571Proton donor By similarity

Natural variations

Natural variant1181A → T in strain: Awamori-1, AKU-4011, K1, K5, NRIC 23, NRIC 1413 and NRIC 1685. Ref.6
Natural variant1991I → V in strain: Pasteur Red. Ref.6
Natural variant2171G → C in strain: YJM326. Ref.2
Natural variant2531T → S in strain: Levuline ALS, Lalvin CY-3079, Cote des Blancs, I14, M1, M11, M12, M13, M15, M2, M20, M21, M22, M24, M29, M3, M30, M31, M32, M33, M34, M4, M5, M6, M7, M8, M9, NRRL Y-1438, NRRL YB-1952, NRRL Y-2411, Pasteur Red, UCD 51, UCD 2120, UCD 175, UCD 529, UCD 765, UCD 781, UCD 820, UCD 762, YJM269, YJM270, YJM308, YJM326, YJM434 and YJM1129. Ref.2 Ref.6
Natural variant3101N → D in strain: SK1, V1-09, YJM269, YJM270, YJM280, YJM320, YJM326, YJM339, YJM627 and YJM1129. Ref.2
Natural variant5411I → V in strain: YJM269 and YJM270. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P30952 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 98E698E86E59C480

FASTA55462,791
        10         20         30         40         50         60 
MVKVSLDNVK LLVDVDKEPF FKPSSTTVGD ILTKDALEFI VLLHRTFNNK RKQLLENRQV 

        70         80         90        100        110        120 
VQKKLDSGSY HLDFLPETAN IRNDPTWQGP ILAPGLINRS TEITGPPLRN MLINALNAPV 

       130        140        150        160        170        180 
NTYMTDFEDS ASPTWNNMVY GQVNLYDAIR NQIDFDTPRK SYKLNGNVAN LPTIIVRPRG 

       190        200        210        220        230        240 
WHMVEKHLYV DDEPISASIF DFGLYFYHNA KELIKLGKGP YFYLPKMEHH LEAKLWNDVF 

       250        260        270        280        290        300 
CVAQDYIGIP RGTIRATVLI ETLPAAFQME EIIYQLRQHS SGLNCGRWDY IFSTIKRLRN 

       310        320        330        340        350        360 
DPNHILPNRN QVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD PAANEKAMTK 

       370        380        390        400        410        420 
VRNDKIRELT NGHDGSWVAH PALAPICNEV FINMGTPNQI YFIPENVVTA ANLLETKIPN 

       430        440        450        460        470        480 
GEITTEGIVQ NLDIGLQYME AWLRGSGCVP INNLMEDAAT AEVSRCQLYQ WVKHGVTLKD 

       490        500        510        520        530        540 
TGEKVTPELT EKILKEQVER LSKASPLGDK NKFALAAKYF LPEIRGEKFS EFLTTLLYDE 

       550 
IVSTKATPTD LSKL 

« Hide

References

« Hide 'large scale' references
[1]"Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism of S. cerevisiae."
Hartig A., Simon M.M., Schuster T., Daugherty J.R., Yoo H.S., Cooper T.G.
Nucleic Acids Res. 20:5677-5686(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast."
Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S., Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H., Steinmetz L.M.
Genetics 180:1661-1670(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS CYS-217; SER-253; ASP-310 AND VAL-541.
Strain: ATCC 200060 / W303, S103, SK1, V1-09, YJM 1129, YJM 269, YJM 270, YJM 320, YJM 326, YJM 339, YJM 627 and YJM230.
[3]"The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames."
de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D., Pallavicini A., Lanfranchi G., Valle G.
Yeast 13:261-266(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Evidence for domesticated and wild populations of Saccharomyces cerevisiae."
Fay J.C., Benavides J.A.
PLoS Genet. 1:66-71(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-282, VARIANTS THR-118; VAL-199 AND SER-253.
Strain: AKU-4011, ATCC 204508 / S288c, ATCC 9804 / CBS 400 / DSM 70478 / IFO 0210 / JCM 2220, Awamori-1, Cote des blancs, I14, K1, K10, K5, K9, Lalvin 71B, Lalvin BM45, Lalvin CY-3079, Levuline ALS, M1, M11, M12, M13, M15, M17, M19, M2, M20, M21, M24, M29, M3, M30, M31, M32, M33, M34, M4, M5, M6, M7, M8, M9, NRIC 1413, NRIC 1685, NRIC 23, NRRL Y-1438, NRRL Y-1532, NRRL Y-1546, NRRL Y-2411, NRRL Y-390, NRRL Y-5997, NRRL Y-7567, NRRL YB-1952, Pasteur Red, UCD 175, UCD 2120, UCD 529, UCD 612, UCD 762, UCD 765, UCD 781, UCD 820, UCD51, YJM 145, YJM 269, YJM 270, YJM 280, YJM 320, YJM 326, YJM 339, YJM 421, YJM 627, YJM1129 YPS163, YJM308, YJM434, YJM436, YJM440, YJM454, YPS1000, YPS1009, Zymaflore F15 and Zymaflore VL3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X64407 Genomic DNA. Translation: CAA45750.1.
EF125216 Genomic DNA. Translation: ABN58540.1.
EF125217 Genomic DNA. Translation: ABN58549.1.
EF125218 Genomic DNA. Translation: ABN58558.1.
EF125219 Genomic DNA. Translation: ABN58567.1.
EF125220 Genomic DNA. Translation: ABN58575.1.
EF125221 Genomic DNA. Translation: ABN58585.1.
EF125222 Genomic DNA. Translation: ABN58594.1.
EF125223 Genomic DNA. Translation: ABN58603.1.
EF125224 Genomic DNA. Translation: ABN58612.1.
EF125225 Genomic DNA. Translation: ABN58621.1.
EF125226 Genomic DNA. Translation: ABN58630.1.
EF125228 Genomic DNA. Translation: ABN58648.1.
Z69382 Genomic DNA. Translation: CAA93390.1.
Z71393 Genomic DNA. Translation: CAA95997.1.
AY942279 Genomic DNA. Translation: AAY22671.1.
AY942280 Genomic DNA. Translation: AAY22672.1.
AY942281 Genomic DNA. Translation: AAY22673.1.
AY942282 Genomic DNA. Translation: AAY22674.1.
AY942283 Genomic DNA. Translation: AAY22675.1.
AY942284 Genomic DNA. Translation: AAY22676.1.
AY942285 Genomic DNA. Translation: AAY22677.1.
AY942286 Genomic DNA. Translation: AAY22678.1.
AY942287 Genomic DNA. Translation: AAY22679.1.
AY942288 Genomic DNA. Translation: AAY22680.1.
AY942289 Genomic DNA. Translation: AAY22681.1.
AY942290 Genomic DNA. Translation: AAY22682.1.
AY942291 Genomic DNA. Translation: AAY22683.1.
AY942292 Genomic DNA. Translation: AAY22684.1.
AY942293 Genomic DNA. Translation: AAY22685.1.
AY942294 Genomic DNA. Translation: AAY22686.1.
AY942295 Genomic DNA. Translation: AAY22687.1.
AY942296 Genomic DNA. Translation: AAY22688.1.
AY942297 Genomic DNA. Translation: AAY22689.1.
AY942298 Genomic DNA. Translation: AAY22690.1.
AY942299 Genomic DNA. Translation: AAY22691.1.
AY942300 Genomic DNA. Translation: AAY22692.1.
AY942301 Genomic DNA. Translation: AAY22693.1.
AY942302 Genomic DNA. Translation: AAY22694.1.
AY942303 Genomic DNA. Translation: AAY22695.1.
AY942304 Genomic DNA. Translation: AAY22696.1.
AY942305 Genomic DNA. Translation: AAY22697.1.
AY942306 Genomic DNA. Translation: AAY22698.1.
AY942307 Genomic DNA. Translation: AAY22699.1.
AY942308 Genomic DNA. Translation: AAY22700.1.
AY942309 Genomic DNA. Translation: AAY22701.1.
AY942310 Genomic DNA. Translation: AAY22702.1.
AY942311 Genomic DNA. Translation: AAY22703.1.
AY942312 Genomic DNA. Translation: AAY22704.1.
AY942313 Genomic DNA. Translation: AAY22705.1.
AY942314 Genomic DNA. Translation: AAY22706.1.
AY942315 Genomic DNA. Translation: AAY22707.1.
AY942316 Genomic DNA. Translation: AAY22708.1.
AY942317 Genomic DNA. Translation: AAY22709.1.
AY942318 Genomic DNA. Translation: AAY22710.1.
AY942319 Genomic DNA. Translation: AAY22711.1.
AY942320 Genomic DNA. Translation: AAY22712.1.
AY942321 Genomic DNA. Translation: AAY22713.1.
AY942322 Genomic DNA. Translation: AAY22714.1.
AY942323 Genomic DNA. Translation: AAY22715.1.
AY942324 Genomic DNA. Translation: AAY22716.1.
AY942325 Genomic DNA. Translation: AAY22717.1.
AY942326 Genomic DNA. Translation: AAY22718.1.
AY942327 Genomic DNA. Translation: AAY22719.1.
AY942328 Genomic DNA. Translation: AAY22720.1.
AY942329 Genomic DNA. Translation: AAY22721.1.
AY942330 Genomic DNA. Translation: AAY22722.1.
AY942331 Genomic DNA. Translation: AAY22723.1.
AY942332 Genomic DNA. Translation: AAY22724.1.
AY942333 Genomic DNA. Translation: AAY22725.1.
AY942334 Genomic DNA. Translation: AAY22726.1.
AY942335 Genomic DNA. Translation: AAY22727.1.
AY942336 Genomic DNA. Translation: AAY22728.1.
AY942337 Genomic DNA. Translation: AAY22729.1.
AY942338 Genomic DNA. Translation: AAY22730.1.
AY942339 Genomic DNA. Translation: AAY22731.1.
AY942340 Genomic DNA. Translation: AAY22732.1.
AY942341 Genomic DNA. Translation: AAY22733.1.
AY942342 Genomic DNA. Translation: AAY22734.1.
AY942343 Genomic DNA. Translation: AAY22735.1.
AY942344 Genomic DNA. Translation: AAY22736.1.
AY942345 Genomic DNA. Translation: AAY22737.1.
AY942346 Genomic DNA. Translation: AAY22738.1.
AY942347 Genomic DNA. Translation: AAY22739.1.
AY942348 Genomic DNA. Translation: AAY22740.1.
AY942349 Genomic DNA. Translation: AAY22741.1.
AY942350 Genomic DNA. Translation: AAY22742.1.
AY942351 Genomic DNA. Translation: AAY22743.1.
AY942352 Genomic DNA. Translation: AAY22744.1.
AY942353 Genomic DNA. Translation: AAY22745.1.
AY942354 Genomic DNA. Translation: AAY22746.1.
AY942355 Genomic DNA. Translation: AAY22747.1.
BK006947 Genomic DNA. Translation: DAA10431.1.
PIRS26645.
RefSeqNP_014282.1. NM_001182955.1.

3D structure databases

ProteinModelPortalP30952.
SMRP30952. Positions 30-539.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35709. 31 interactions.
IntActP30952. 9 interactions.
MINTMINT-4083741.

Proteomic databases

PeptideAtlasP30952.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL117W; YNL117W; YNL117W.
GeneID855606.
KEGGsce:YNL117W.

Organism-specific databases

CYGDYNL117w.
SGDS000005061. MLS1.

Phylogenomic databases

GeneTreeENSGT00390000002932.
KOK01638.
OMAEDANTPH.
OrthoDBEOG7GXPMK.

Enzyme and pathway databases

BioCycMetaCyc:YNL117W-MONOMER.
YEAST:YNL117W-MONOMER.
UniPathwayUPA00703; UER00720.

Gene expression databases

GenevestigatorP30952.

Family and domain databases

InterProIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PANTHERPTHR21631:SF1. PTHR21631:SF1. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001363. Malate_synth. 1 hit.
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01344. malate_syn_A. 1 hit.
PROSITEPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979777.

Entry information

Entry nameMASY_YEAST
AccessionPrimary (citable) accession number: P30952
Secondary accession number(s): B0KZR8 expand/collapse secondary AC list , B0KZS7, B0KZU5, B0KZY1, B0L026, D6W165, Q4KQ64, Q4KQ91, Q4KQC3, Q4KQC4, Q4KQC8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways