Malate synthase 1, glyoxysomal - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P30952 (MASY_YEAST)

Last modified June 16, 2009. Version 80. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Malate synthase 1, glyoxysomal
EC=2.3.3.9

Gene names

Name:	MLS1
Ordered Locus Names:	YNL117W
ORF Names:	N1921

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	This isozyme is necessary for growth on acetate as sole C-source.
Catalytic activity	Acetyl-CoA + H₂O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; L-malate from isocitrate: step 2/2.
Subunit structure	Homotrimer.
Subcellular location	Glyoxysome.
Sequence similarities	Belongs to the malate synthase family.

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Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
itself		1	EBI-10428,EBI-10428
	P43582	1	EBI-10428,EBI-22766
AAT2	P23542	1	EBI-10428,EBI-2002
AUS1	Q08409	1	EBI-10428,EBI-35723
ESS1	P22696	1	EBI-10428,EBI-6679
PGM2	P37012	1	EBI-10428,EBI-13296
PRP40	P33203	1	EBI-10428,EBI-701
RNR1	P21524	1	EBI-10428,EBI-15234
RSP5	P39940	1	EBI-10428,EBI-16219
SSM4	P40318	1	EBI-10428,EBI-18208
URN1	Q06525	1	EBI-10428,EBI-35138
VID30	P53076	1	EBI-10428,EBI-24173

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 554

554

Malate synthase 1, glyoxysomal

PRO_0000166864

Regions

Motif

552 – 554

Microbody targeting signal Potential

Sites

Active site

177

Proton acceptor By similarity

Active site

457

Proton donor By similarity

Natural variations

Natural variant

118

A → T in strain: Awamori-1, AKU-4011, K1, K5, NRIC 23, NRIC 1413 and NRIC 1685.

Natural variant

199

I → V in strain: Pasteur Red.

Natural variant

217

G → C in strain: YJM326.

Natural variant

253

T → S in strain: Levuline ALS, Lalvin CY-3079, Cote des Blancs, I14, M1, M11, M12, M13, M15, M2, M20, M21, M22, M24, M29, M3, M30, M31, M32, M33, M34, M4, M5, M6, M7, M8, M9, NRRL Y-1438, NRRL YB-1952, NRRL Y-2411, Pasteur Red, UCD 51, UCD 2120, UCD 175, UCD 529, UCD 765, UCD 781, UCD 820, UCD 762, YJM269, YJM270, YJM308, YJM326, YJM434 and YJM1129.

Natural variant

310

N → D in strain: SK1, V1-09, YJM269, YJM270, YJM280, YJM320, YJM326, YJM339, YJM627 and YJM1129.

Natural variant

541

I → V in strain: YJM269 and YJM270.

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Sequences

Sequence

Length

Mass (Da)

Tools

P30952-1 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 98E698E86E59C480
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FASTA

554

62,791

        10         20         30         40         50         60 
MVKVSLDNVK LLVDVDKEPF FKPSSTTVGD ILTKDALEFI VLLHRTFNNK RKQLLENRQV 

        70         80         90        100        110        120 
VQKKLDSGSY HLDFLPETAN IRNDPTWQGP ILAPGLINRS TEITGPPLRN MLINALNAPV 

       130        140        150        160        170        180 
NTYMTDFEDS ASPTWNNMVY GQVNLYDAIR NQIDFDTPRK SYKLNGNVAN LPTIIVRPRG 

       190        200        210        220        230        240 
WHMVEKHLYV DDEPISASIF DFGLYFYHNA KELIKLGKGP YFYLPKMEHH LEAKLWNDVF 

       250        260        270        280        290        300 
CVAQDYIGIP RGTIRATVLI ETLPAAFQME EIIYQLRQHS SGLNCGRWDY IFSTIKRLRN 

       310        320        330        340        350        360 
DPNHILPNRN QVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD PAANEKAMTK 

       370        380        390        400        410        420 
VRNDKIRELT NGHDGSWVAH PALAPICNEV FINMGTPNQI YFIPENVVTA ANLLETKIPN 

       430        440        450        460        470        480 
GEITTEGIVQ NLDIGLQYME AWLRGSGCVP INNLMEDAAT AEVSRCQLYQ WVKHGVTLKD 

       490        500        510        520        530        540 
TGEKVTPELT EKILKEQVER LSKASPLGDK NKFALAAKYF LPEIRGEKFS EFLTTLLYDE 

       550 
IVSTKATPTD LSKL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism of S. cerevisiae." Hartig A., Simon M.M., Schuster T., Daugherty J.R., Yoo H.S., Cooper T.G. Nucleic Acids Res. 20:5677-5686(1992) [PubMed: 1454530] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast." Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S., Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H., Steinmetz L.M. Genetics 180:1661-1670(2008) [PubMed: 18780730] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS CYS-217; SER-253; ASP-310 AND VAL-541. Strain: ATCC 200060 / W303, S103, SK1, V1-09, YJM1129, YJM230, YJM269, YJM270, YJM320, YJM326, YJM339 and YJM627.
[3]	"The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames." de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D., Pallavicini A., Lanfranchi G., Valle G. Yeast 13:261-266(1997) [PubMed: 9090055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Evidence for domesticated and wild populations of Saccharomyces cerevisiae." Fay J.C., Benavides J.A. PLoS Genet. 1:66-71(2005) [PubMed: 16103919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-282, VARIANTS THR-118; VAL-199 AND SER-253. Strain: AKU-4011, Awamori-1, Cote des blancs, I14, K1, K10, K5, K9, Lalvin 71B, Lalvin BM45, Lalvin CY-3079, Levuline ALS, M1, M11, M12, M13, M15, M17, M19, M2, M20, M21, M24, M29, M3, M30, M31, M32, M33, M34, M4, M5, M6, M7, M8, M9, NRIC 1413, NRIC 1685, NRIC 23, NRRL Y-12633, NRRL Y-1438, NRRL Y-1532, NRRL Y-1546, NRRL Y-2411, NRRL Y-390, NRRL Y-5997, NRRL Y-7567, NRRL YB-1952, Pasteur Red, S288C, UCD 175, UCD 2120, UCD 529, UCD 612, UCD 762, UCD 765, UCD 781, UCD 820, UCD51, YJM 339, YJM 421, YJM1129 YPS163, YJM145, YJM269, YJM270, YJM280, YJM308, YJM320, YJM326, YJM434, YJM436, YJM440, YJM454, YJM627, YPS1000, YPS1009, Zymaflore F15 and Zymaflore VL3.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X64407 Genomic DNA. Translation: CAA45750.1. EF125216 Genomic DNA. Translation: ABN58540.1. EF125217 Genomic DNA. Translation: ABN58549.1. EF125218 Genomic DNA. Translation: ABN58558.1. EF125219 Genomic DNA. Translation: ABN58567.1. EF125220 Genomic DNA. Translation: ABN58575.1. EF125221 Genomic DNA. Translation: ABN58585.1. EF125222 Genomic DNA. Translation: ABN58594.1. EF125223 Genomic DNA. Translation: ABN58603.1. EF125224 Genomic DNA. Translation: ABN58612.1. EF125225 Genomic DNA. Translation: ABN58621.1. EF125226 Genomic DNA. Translation: ABN58630.1. EF125228 Genomic DNA. Translation: ABN58648.1. Z69382 Genomic DNA. Translation: CAA93390.1. Z71393 Genomic DNA. Translation: CAA95997.1. AY942279 Genomic DNA. Translation: AAY22671.1. AY942280 Genomic DNA. Translation: AAY22672.1. AY942281 Genomic DNA. Translation: AAY22673.1. AY942282 Genomic DNA. Translation: AAY22674.1. AY942283 Genomic DNA. Translation: AAY22675.1. AY942284 Genomic DNA. Translation: AAY22676.1. AY942285 Genomic DNA. Translation: AAY22677.1. AY942286 Genomic DNA. Translation: AAY22678.1. AY942287 Genomic DNA. Translation: AAY22679.1. AY942288 Genomic DNA. Translation: AAY22680.1. AY942289 Genomic DNA. Translation: AAY22681.1. AY942290 Genomic DNA. Translation: AAY22682.1. AY942291 Genomic DNA. Translation: AAY22683.1. AY942292 Genomic DNA. Translation: AAY22684.1. AY942293 Genomic DNA. Translation: AAY22685.1. AY942294 Genomic DNA. Translation: AAY22686.1. AY942295 Genomic DNA. Translation: AAY22687.1. AY942296 Genomic DNA. Translation: AAY22688.1. AY942297 Genomic DNA. Translation: AAY22689.1. AY942298 Genomic DNA. Translation: AAY22690.1. AY942299 Genomic DNA. Translation: AAY22691.1. AY942300 Genomic DNA. Translation: AAY22692.1. AY942301 Genomic DNA. Translation: AAY22693.1. AY942302 Genomic DNA. Translation: AAY22694.1. AY942303 Genomic DNA. Translation: AAY22695.1. AY942304 Genomic DNA. Translation: AAY22696.1. AY942305 Genomic DNA. Translation: AAY22697.1. AY942306 Genomic DNA. Translation: AAY22698.1. AY942307 Genomic DNA. Translation: AAY22699.1. AY942308 Genomic DNA. Translation: AAY22700.1. AY942309 Genomic DNA. Translation: AAY22701.1. AY942310 Genomic DNA. Translation: AAY22702.1. AY942311 Genomic DNA. Translation: AAY22703.1. AY942312 Genomic DNA. Translation: AAY22704.1. AY942313 Genomic DNA. Translation: AAY22705.1. AY942314 Genomic DNA. Translation: AAY22706.1. AY942315 Genomic DNA. Translation: AAY22707.1. AY942316 Genomic DNA. Translation: AAY22708.1. AY942317 Genomic DNA. Translation: AAY22709.1. AY942318 Genomic DNA. Translation: AAY22710.1. AY942319 Genomic DNA. Translation: AAY22711.1. AY942320 Genomic DNA. Translation: AAY22712.1. AY942321 Genomic DNA. Translation: AAY22713.1. AY942322 Genomic DNA. Translation: AAY22714.1. AY942323 Genomic DNA. Translation: AAY22715.1. AY942324 Genomic DNA. Translation: AAY22716.1. AY942325 Genomic DNA. Translation: AAY22717.1. AY942326 Genomic DNA. Translation: AAY22718.1. AY942327 Genomic DNA. Translation: AAY22719.1. AY942328 Genomic DNA. Translation: AAY22720.1. AY942329 Genomic DNA. Translation: AAY22721.1. AY942330 Genomic DNA. Translation: AAY22722.1. AY942331 Genomic DNA. Translation: AAY22723.1. AY942332 Genomic DNA. Translation: AAY22724.1. AY942333 Genomic DNA. Translation: AAY22725.1. AY942334 Genomic DNA. Translation: AAY22726.1. AY942335 Genomic DNA. Translation: AAY22727.1. AY942336 Genomic DNA. Translation: AAY22728.1. AY942337 Genomic DNA. Translation: AAY22729.1. AY942338 Genomic DNA. Translation: AAY22730.1. AY942339 Genomic DNA. Translation: AAY22731.1. AY942340 Genomic DNA. Translation: AAY22732.1. AY942341 Genomic DNA. Translation: AAY22733.1. AY942342 Genomic DNA. Translation: AAY22734.1. AY942343 Genomic DNA. Translation: AAY22735.1. AY942344 Genomic DNA. Translation: AAY22736.1. AY942345 Genomic DNA. Translation: AAY22737.1. AY942346 Genomic DNA. Translation: AAY22738.1. AY942347 Genomic DNA. Translation: AAY22739.1. AY942348 Genomic DNA. Translation: AAY22740.1. AY942349 Genomic DNA. Translation: AAY22741.1. AY942350 Genomic DNA. Translation: AAY22742.1. AY942351 Genomic DNA. Translation: AAY22743.1. AY942352 Genomic DNA. Translation: AAY22744.1. AY942353 Genomic DNA. Translation: AAY22745.1. AY942354 Genomic DNA. Translation: AAY22746.1. AY942355 Genomic DNA. Translation: AAY22747.1.
PIR	S26645.
RefSeq	NP_014282.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P30952. 16 interactions.
Proteomic databases
PeptideAtlas	P30952.
Genome annotation databases
Ensembl	YNL117W. Saccharomyces cerevisiae. [Contig view]
GeneID	855606.
NMPDR	fig\|4932.3.peg.5352.
Organism-specific databases
CYGD	YNL117w.
SGD	S000005061. MLS1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P30952.
OMA	P30952. TANIRND.
Enzyme and pathway databases
BRENDA	2.3.3.9. 250.
Gene expression databases
ArrayExpress	P30952.
GermOnline	YNL117W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011076. Malate_synth-like_core. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979777.

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Entry information

Entry name

MASY_YEAST

Accession

Primary (citable) accession number: P30952
Secondary accession number(s): B0KZR8

Q4KQC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents