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P30951 (GPR12_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor 12
Alternative name(s):
R334
Gene names
Name:Gpr12
Synonyms:Gpcr12
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor with constitutive G(s) signaling activity that activates cyclic AMP. Promotes neurite outgrowth and blocks myelin inhibition in neurons. Ref.4

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Tissue specificity

Expressed in the brain, pituitary gland and testis. Ref.1 Ref.4

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence caution

The sequence CAA43713.1 differs from that shown. Reason: Frameshift at positions 151, 160, 175, 177, 193, 311 and 325.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334G-protein coupled receptor 12
PRO_0000069529

Regions

Topological domain1 – 4848Extracellular Potential
Transmembrane49 – 6921Helical; Name=1; Potential
Topological domain70 – 789Cytoplasmic Potential
Transmembrane79 – 9921Helical; Name=2; Potential
Topological domain100 – 11314Extracellular Potential
Transmembrane114 – 13421Helical; Name=3; Potential
Topological domain135 – 15824Cytoplasmic Potential
Transmembrane159 – 17921Helical; Name=4; Potential
Topological domain180 – 19920Extracellular Potential
Transmembrane200 – 22021Helical; Name=5; Potential
Topological domain221 – 25232Cytoplasmic Potential
Transmembrane253 – 27321Helical; Name=6; Potential
Topological domain274 – 2829Extracellular Potential
Transmembrane283 – 30321Helical; Name=7; Potential
Topological domain304 – 33431Cytoplasmic Potential

Amino acid modifications

Modified residue3301Phosphoserine Potential
Modified residue3321Phosphoserine Potential
Lipidation3171S-palmitoyl cysteine By similarity
Glycosylation81N-linked (GlcNAc...) Potential
Glycosylation241N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P30951 [UniParc].

Last modified October 1, 1996. Version 3.
Checksum: DFA50BEA56141E44

FASTA33436,704
        10         20         30         40         50         60 
MNEDPKVNLS GLPRDCIEAG TPENISAAVP SQGSVVESEP ELVVNPWDIV LCSSGTLICC 

        70         80         90        100        110        120 
ENAVVVLIIF HSPSLRAPMF LLIGSLALAD LLAGLGLIIN FVFAYLLQSE ATKLVTIGLI 

       130        140        150        160        170        180 
VASFSASVCS LLAITVDRYL SLYYALTYHS ERTVTFTYVM LVMLWGTSTC LGLLPVMGWN 

       190        200        210        220        230        240 
CLRDESTCSV VRPLTKNNAA ILSISFLFMF ALMLQLYIQI CKIVMRHAHQ IALQHHFLAT 

       250        260        270        280        290        300 
SHYVTTRKGI STLALILGTF AACWMPFTLY SLIADYTYPS IYTYATLLPA TYNSIINPVI 

       310        320        330 
YAFRNQEIQK ALCLICCGCI PNTLSQRARS PSDV 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing and tissue distribution of a candidate G protein-coupled receptor from rat pituitary gland."
Eidne K.A., Zabavnik J., Peters T., Yoshida S., Anderson L., Taylor P.L.
FEBS Lett. 292:243-248(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Pituitary.
[2]"A putative rat G protein coupled receptor cDNA isolated from cerebral cortex."
Bonner T.I., Brownstein M.J.
Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth."
Tanaka S., Ishii K., Kasai K., Yoon S.O., Saeki Y.
J. Biol. Chem. 282:10506-10515(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61496 mRNA. Translation: CAA43713.1. Frameshift.
U12184 mRNA. Translation: AAB60518.1.
BC127447 mRNA. Translation: AAI27448.1.
PIRS18444.
RefSeqNP_001032372.1. NM_001037295.1.
NP_110458.1. NM_030831.1.
XP_006248901.1. XM_006248839.1.
UniGeneRn.88417.

3D structure databases

ProteinModelPortalP30951.
ModBaseSearch...
MobiDBSearch...

Chemistry

GuidetoPHARMACOLOGY86.

Protein family/group databases

GPCRDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000061129; ENSRNOP00000057844; ENSRNOG00000039832.
GeneID80840.
KEGGrno:80840.

Organism-specific databases

CTD2835.
RGD68333. Gpr12.

Phylogenomic databases

eggNOGNOG309471.
GeneTreeENSGT00750000117639.
HOGENOMHOG000294075.
HOVERGENHBG079561.
KOK04315.
OMALTYNSER.
OrthoDBEOG7966H0.
PhylomeDBP30951.

Gene expression databases

GenevestigatorP30951.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000599. GPR12.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00650. GPR12ORPHANR.
PR00644. GPRORPHANR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio614870.
PROP30951.

Entry information

Entry nameGPR12_RAT
AccessionPrimary (citable) accession number: P30951
Secondary accession number(s): A0JPJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries