P30951 (GPR12_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G-protein coupled receptor 12

Alternative name(s):
R334

Gene names

Name:	Gpr12
Synonyms:	Gpcr12

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor with constitutive G(s) signaling activity that activates cyclic AMP. Promotes neurite outgrowth and blocks myelin inhibition in neurons. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed in the brain, pituitary gland and testis. Ref.1 Ref.4
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	The sequence CAA43713.1 differs from that shown. Reason: Frameshift at positions 151, 160, 175, 177, 193, 311 and 325.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular calcium ion homeostasis Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylcholine binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	G-protein coupled receptor 12		PRO_0000069529
Regions
	Topological domain	1 – 48	48	Extracellular Potential
	Transmembrane	49 – 69	21	Helical; Name=1; Potential
	Topological domain	70 – 78	9	Cytoplasmic Potential
	Transmembrane	79 – 99	21	Helical; Name=2; Potential
	Topological domain	100 – 113	14	Extracellular Potential
	Transmembrane	114 – 134	21	Helical; Name=3; Potential
	Topological domain	135 – 158	24	Cytoplasmic Potential
	Transmembrane	159 – 179	21	Helical; Name=4; Potential
	Topological domain	180 – 199	20	Extracellular Potential
	Transmembrane	200 – 220	21	Helical; Name=5; Potential
	Topological domain	221 – 252	32	Cytoplasmic Potential
	Transmembrane	253 – 273	21	Helical; Name=6; Potential
	Topological domain	274 – 282	9	Extracellular Potential
	Transmembrane	283 – 303	21	Helical; Name=7; Potential
	Topological domain	304 – 334	31	Cytoplasmic Potential
Amino acid modifications
	Modified residue	330	1	Phosphoserine Potential
	Modified residue	332	1	Phosphoserine Potential
	Lipidation	317	1	S-palmitoyl cysteine By similarity
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P30951 [UniParc]. Last modified October 1, 1996. Version 3. Checksum: DFA50BEA56141E44	FASTA	334	36,704
	10 20 30 40 50 60 MNEDPKVNLS GLPRDCIEAG TPENISAAVP SQGSVVESEP ELVVNPWDIV LCSSGTLICC 70 80 90 100 110 120 ENAVVVLIIF HSPSLRAPMF LLIGSLALAD LLAGLGLIIN FVFAYLLQSE ATKLVTIGLI 130 140 150 160 170 180 VASFSASVCS LLAITVDRYL SLYYALTYHS ERTVTFTYVM LVMLWGTSTC LGLLPVMGWN 190 200 210 220 230 240 CLRDESTCSV VRPLTKNNAA ILSISFLFMF ALMLQLYIQI CKIVMRHAHQ IALQHHFLAT 250 260 270 280 290 300 SHYVTTRKGI STLALILGTF AACWMPFTLY SLIADYTYPS IYTYATLLPA TYNSIINPVI 310 320 330 YAFRNQEIQK ALCLICCGCI PNTLSQRARS PSDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and tissue distribution of a candidate G protein-coupled receptor from rat pituitary gland." Eidne K.A., Zabavnik J., Peters T., Yoshida S., Anderson L., Taylor P.L. FEBS Lett. 292:243-248(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Pituitary.
[2]	"A putative rat G protein coupled receptor cDNA isolated from cerebral cortex." Bonner T.I., Brownstein M.J. Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth." Tanaka S., Ishii K., Kasai K., Yoon S.O., Saeki Y. J. Biol. Chem. 282:10506-10515(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61496 mRNA. Translation: CAA43713.1. Frameshift. U12184 mRNA. Translation: AAB60518.1. BC127447 mRNA. Translation: AAI27448.1.
IPI	IPI00213706.
PIR	S18444.
RefSeq	NP_001032372.1. NM_001037295.1. NP_110458.1. NM_030831.1.
UniGene	Rn.88417.
3D structure databases
ProteinModelPortal	P30951.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000061129; ENSRNOP00000057844; ENSRNOG00000039832.
GeneID	80840.
KEGG	rno:80840.
Organism-specific databases
CTD	2835.
RGD	68333. Gpr12.
Phylogenomic databases
eggNOG	NOG309471.
GeneTree	ENSGT00700000104085.
HOGENOM	HOG000294075.
HOVERGEN	HBG079561.
KO	K04315.
OrthoDB	EOG4MSD02.
Gene expression databases
Genevestigator	P30951.
GermOnline	ENSRNOG00000039832. Rattus norvegicus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000599. GPR12. IPR000723. GPR_3/6/12_orphan. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00650. GPR12ORPHANR. PR00644. GPRORPHANR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	614870.

Entry information

Entry name

GPR12_RAT

Accession

Primary (citable) accession number: P30951
Secondary accession number(s): A0JPJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program