P30943 (PHE2_RHDS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phycoerythrin alpha-2 chain, chloroplastic

Gene names

Name:

cpeA2

Organism

Rhodomonas sp. (strain CS 24) (Chroomonas sp. (strain CS24))

Taxonomic identifier

79257 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Cryptophyta › Pyrenomonadales › Pyrenomonadaceae › Rhodomonas

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Light-harvesting photosynthetic tetrapyrrole chromophore-protein from the phycobiliprotein complex.
Subunit structure	Heterotetramer of 2 different alpha chains and 2 identical beta chains. The subunit composition could comprise of any combination of 2 out of 4 different alpha units with an invariant beta unit. Ref.3 Ref.4
Subcellular location	Plastid › chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side.
Post-translational modification	Contains one covalently linked 15,16-dihydrobiliverdin chromophore.
Miscellaneous	The light-harvesting system in Cryptophytes contains phycobiliprotein complexes. Unusually they are composed of either phycoerythrin (CPE) or phycocyanin (CPC) but never allophycocyanin (APC), with only one type of biliprotein being present in any one species. Unlike cyanobacteria or red algae these proteins are not arranged into higher-order phycobilisome complexes, and they are found in the thylakoid lumen.
Sequence similarities	Belongs to the phycoerythrin family.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide
Ligand	Bile pigment Chromophore
PTM	Hydroxylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell phycobilisome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 37

37

Chloroplast Ref.2

Chain

38 – 104

67

Phycoerythrin alpha-2 chain, chloroplastic

PRO_0000002827

Regions

Region

61 – 63

3

15,16-dihydrobiliverdin chromophore

Sites

Binding site

56

1

15,16-dihydrobiliverdin chromophore (covalent; via 1 link)

Binding site

58

1

15,16-dihydrobiliverdin chromophore

Binding site

78

1

15,16-dihydrobiliverdin chromophore

Amino acid modifications

Modified residue

41

1

5-hydroxylysine Ref.3

Experimental info

Sequence conflict

63 – 74

12

YTGAK…GKDDE → TSKSGKSGQDDT AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

104

Helix Strand Turn

Details...

Beta strand

44 – 52

9

Helix

71 – 74

4

Beta strand

75 – 83

9

Helix

88 – 101

14

Sequences

	Sequence		Length	Mass (Da)
	P30943 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: 1474D433E8CB4396	FASTA	104	10,696
	10 20 30 40 50 60 MSAKIIAFSA VVATASAFAP TAGFVPRLRS GATSVNMAMD KSAKAPVITI FDHRGCSRAP 70 80 90 100 KEYTGAKAGG KDDEMMVKAQ SVKIEVSTGT AEGVLATSLA KMTK

References

[1]	"Nuclear-encoded genes of phycoerythrin alpha subunits." Hiller R.G., Howe C.E. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A genomic clone encoding a cryptophyte phycoerythrin alpha-subunit. Evidence for three alpha-subunits and an N-terminal membrane transit sequence." Jenkins J., Hiller R.G., Speirs J., Godovac-Zimmermann J. FEBS Lett. 273:191-194(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 38-82.
[3]	"Evolution of a light-harvesting protein by addition of new subunits and rearrangement of conserved elements: crystal structure of a cryptophyte phycoerythrin at 1.63-A resolution." Wilk K.E., Harrop S.J., Jankova L., Edler D., Keenan G., Sharples F., Hiller R.G., Curmi P.M. Proc. Natl. Acad. Sci. U.S.A. 96:8901-8906(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.63 ANGSTROMS) OF 38-104 IN COMPLEX WITH CPEA2; CPEA3 AND 15,16-DIHYDROBILIVERDIN, SUBUNIT, HYDROXYLATION AT LYS-41.
[4]	"Developing a structure-function model for the cryptophyte phycoerythrin 545 using ultrahigh resolution crystallography and ultrafast laser spectroscopy." Doust A.B., Marai C.N., Harrop S.J., Wilk K.E., Curmi P.M., Scholes G.D. J. Mol. Biol. 344:135-153(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (0.97 ANGSTROMS) OF 38-104 IN COMPLEX WITH CPEA2; CPEA3 AND 15,16-DIHYDROBILIVERDIN, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ006994 Genomic DNA. Translation: CAD20039.1.

PIR

T10881.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QGW	X-ray	1.63	B	38-104	[»]
1XF6	X-ray	1.10	B	38-104	[»]
1XG0	X-ray	0.97	B	38-104	[»]

ProteinModelPortal

P30943.

SMR

P30943. Positions 38-104.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.90.510.10. 1 hit.

InterPro

IPR011070. Globular_prot_asu/bsu.
IPR004228. Phycoerythr_a/b_core.
[Graphical view]

Pfam

PF02972. Phycoerythr_ab. 1 hit.
[Graphical view]

ProDom

PD019398. Phycoerythr_a/b_core. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF56568. AlphaBeta_subunt. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P30943.

Entry information

Entry name

PHE2_RHDS2

Accession

Primary (citable) accession number: P30943
Secondary accession number(s): Q8VX02

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	May 27, 2002
Last modified:	April 3, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)