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P30943

- PHE2_RHDS2

UniProt

P30943 - PHE2_RHDS2

Protein

Phycoerythrin alpha-2 chain, chloroplastic

Gene

cpeA2

Organism
Rhodomonas sp. (strain CS 24) (Chroomonas sp. (strain CS24))
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Light-harvesting photosynthetic tetrapyrrole chromophore-protein from the phycobiliprotein complex.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei56 – 56115,16-dihydrobiliverdin chromophore (covalent; via 1 link)2 Publications
    Binding sitei58 – 58115,16-dihydrobiliverdin chromophore2 Publications
    Binding sitei78 – 78115,16-dihydrobiliverdin chromophore2 Publications

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB-KW
    2. photosynthesis Source: UniProtKB-KW
    3. protein-chromophore linkage Source: UniProtKB-KW

    Keywords - Biological processi

    Electron transport, Photosynthesis, Transport

    Keywords - Ligandi

    Bile pigment, Chromophore

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phycoerythrin alpha-2 chain, chloroplastic
    Gene namesi
    Name:cpeA2
    OrganismiRhodomonas sp. (strain CS 24) (Chroomonas sp. (strain CS24))
    Taxonomic identifieri79257 [NCBI]
    Taxonomic lineageiEukaryotaCryptophytaPyrenomonadalesPyrenomonadaceaeRhodomonas

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast thylakoid membrane Source: UniProtKB-SubCell
    2. phycobilisome Source: InterPro

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Thylakoid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3737Chloroplast1 PublicationAdd
    BLAST
    Chaini38 – 10467Phycoerythrin alpha-2 chain, chloroplasticPRO_0000002827Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei41 – 4115-hydroxylysine1 Publication

    Post-translational modificationi

    Contains one covalently linked 15,16-dihydrobiliverdin chromophore.

    Keywords - PTMi

    Hydroxylation

    Interactioni

    Subunit structurei

    Heterotetramer of 2 different alpha chains and 2 identical beta chains. The subunit composition could comprise of any combination of 2 out of 4 different alpha units with an invariant beta unit.2 Publications

    Structurei

    Secondary structure

    1
    104
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi44 – 529
    Helixi71 – 744
    Beta strandi75 – 839
    Helixi88 – 10114

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1QGWX-ray1.63B38-104[»]
    1XF6X-ray1.10B38-104[»]
    1XG0X-ray0.97B38-104[»]
    ProteinModelPortaliP30943.
    SMRiP30943. Positions 38-104.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP30943.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni61 – 63315,16-dihydrobiliverdin chromophore

    Sequence similaritiesi

    Belongs to the phycoerythrin family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.90.510.10. 1 hit.
    InterProiIPR011070. Globular_prot_asu/bsu.
    IPR004228. Phycoerythr_a_core.
    [Graphical view]
    PfamiPF02972. Phycoerythr_ab. 1 hit.
    [Graphical view]
    ProDomiPD019398. Phycoerythr_a/b_core. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF56568. SSF56568. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30943-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAKIIAFSA VVATASAFAP TAGFVPRLRS GATSVNMAMD KSAKAPVITI    50
    FDHRGCSRAP KEYTGAKAGG KDDEMMVKAQ SVKIEVSTGT AEGVLATSLA 100
    KMTK 104
    Length:104
    Mass (Da):10,696
    Last modified:May 27, 2002 - v2
    Checksum:i1474D433E8CB4396
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti63 – 7412YTGAK…GKDDE → TSKSGKSGQDDT AA sequence (PubMed:2226853)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ006994 Genomic DNA. Translation: CAD20039.1.
    PIRiT10881.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ006994 Genomic DNA. Translation: CAD20039.1 .
    PIRi T10881.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1QGW X-ray 1.63 B 38-104 [» ]
    1XF6 X-ray 1.10 B 38-104 [» ]
    1XG0 X-ray 0.97 B 38-104 [» ]
    ProteinModelPortali P30943.
    SMRi P30943. Positions 38-104.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P30943.

    Family and domain databases

    Gene3Di 3.90.510.10. 1 hit.
    InterProi IPR011070. Globular_prot_asu/bsu.
    IPR004228. Phycoerythr_a_core.
    [Graphical view ]
    Pfami PF02972. Phycoerythr_ab. 1 hit.
    [Graphical view ]
    ProDomi PD019398. Phycoerythr_a/b_core. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF56568. SSF56568. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nuclear-encoded genes of phycoerythrin alpha subunits."
      Hiller R.G., Howe C.E.
      Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A genomic clone encoding a cryptophyte phycoerythrin alpha-subunit. Evidence for three alpha-subunits and an N-terminal membrane transit sequence."
      Jenkins J., Hiller R.G., Speirs J., Godovac-Zimmermann J.
      FEBS Lett. 273:191-194(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 38-82.
    3. "Evolution of a light-harvesting protein by addition of new subunits and rearrangement of conserved elements: crystal structure of a cryptophyte phycoerythrin at 1.63-A resolution."
      Wilk K.E., Harrop S.J., Jankova L., Edler D., Keenan G., Sharples F., Hiller R.G., Curmi P.M.
      Proc. Natl. Acad. Sci. U.S.A. 96:8901-8906(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.63 ANGSTROMS) OF 38-104 IN COMPLEX WITH CPEA2; CPEA3 AND 15,16-DIHYDROBILIVERDIN, SUBUNIT, HYDROXYLATION AT LYS-41.
    4. "Developing a structure-function model for the cryptophyte phycoerythrin 545 using ultrahigh resolution crystallography and ultrafast laser spectroscopy."
      Doust A.B., Marai C.N., Harrop S.J., Wilk K.E., Curmi P.M., Scholes G.D.
      J. Mol. Biol. 344:135-153(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (0.97 ANGSTROMS) OF 38-104 IN COMPLEX WITH CPEA2; CPEA3 AND 15,16-DIHYDROBILIVERDIN, SUBUNIT.

    Entry informationi

    Entry nameiPHE2_RHDS2
    AccessioniPrimary (citable) accession number: P30943
    Secondary accession number(s): Q8VX02
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: May 27, 2002
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    The light-harvesting system in Cryptophytes contains phycobiliprotein complexes. Unusually they are composed of either phycoerythrin (CPE) or phycocyanin (CPC) but never allophycocyanin (APC), with only one type of biliprotein being present in any one species. Unlike cyanobacteria or red algae these proteins are not arranged into higher-order phycobilisome complexes, and they are found in the thylakoid lumen.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3