P30938 (SSR5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Somatostatin receptor type 5
Short name=SS-5-R
Short name=SS5-R
Short name=SS5R

Gene names

Name:

Sstr5

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for somatostatin-28. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Prominent in the pituitary and small intestine. Low levels in islets and spleen. Not detected in kidney, pancreas, cerebellum, or cortex.
Post-translational modification	Palmitoylated at Cys-320 by ZDHHC5, but not ZDHHC8. Palmitoylation creates an additional intracellular loop which is thought to be important for efficient coupling to G-proteins and may target the protein to lipid rafts By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Inferred from direct assay Ref.1. Source: RGD cellular response to glucocorticoid stimulus Inferred from expression pattern PubMed 14512709. Source: RGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 9322965. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 9322965. Source: RGD
Molecular_function	somatostatin receptor activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Somatostatin receptor type 5		PRO_0000070132
Regions
	Topological domain	1 – 35	35	Extracellular Potential
	Transmembrane	36 – 63	28	Helical; Name=1; Potential
	Topological domain	64 – 73	10	Cytoplasmic Potential
	Transmembrane	74 – 99	26	Helical; Name=2; Potential
	Topological domain	100 – 111	12	Extracellular Potential
	Transmembrane	112 – 133	22	Helical; Name=3; Potential
	Topological domain	134 – 155	22	Cytoplasmic Potential
	Transmembrane	156 – 176	21	Helical; Name=4; Potential
	Topological domain	177 – 196	20	Extracellular Potential
	Transmembrane	197 – 221	25	Helical; Name=5; Potential
	Topological domain	222 – 247	26	Cytoplasmic Potential
	Transmembrane	248 – 273	26	Helical; Name=6; Potential
	Topological domain	274 – 283	10	Extracellular Potential
	Transmembrane	284 – 308	25	Helical; Name=7; Potential
	Topological domain	309 – 363	55	Cytoplasmic Potential
Amino acid modifications
	Lipidation	320	1	S-palmitoyl cysteine; by ZDHHC5 By similarity
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Glycosylation	186	1	N-linked (GlcNAc...) Potential
	Disulfide bond	110 ↔ 185		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30938 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 4BD4512960613B4A	FASTA	363	39,971
	10 20 30 40 50 60 MEPLSLASTP SWNASAASSG NHNWSLVGSA SPMGARAVLV PVLYLLVCTV GLSGNTLVIY 70 80 90 100 110 120 VVLRHAKMKT VTNVYILNLA VADVLFMLGL PFLATQNAVV SYWPFGSFLC RLVMTLDGIN 130 140 150 160 170 180 QFTSIFCLMV MSVDRYLAVV HPLRSARWRR PRVAKMASAA VWVFSLLMSL PLLVFADVQE 190 200 210 220 230 240 GWGTCNLSWP EPVGLWGAAF ITYTSVLGFF GPLLVICLCY LLIVVKVKAA GMRVGSSRRR 250 260 270 280 290 300 RSEPKVTRMV VVVVLVFVGC WLPFFIVNIV NLAFTLPEEP TSAGLYFFVV VLSYANSCAN 310 320 330 340 350 360 PLLYGFLSDN FRQSFRKVLC LRRGYGMEDA DAIEPRPDKS GRPQATLPTR SCEANGLMQT SRI

References

[1]	"Molecular cloning and expression of a pituitary somatostatin receptor with preferential affinity for somatostatin-28." O'Carroll A.-M., Lolait S.J., Konig M., Mahan L.C. Mol. Pharmacol. 42:939-946(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.
[2]	"Molecular cloning, functional characterization, and chromosomal localization of a human somatostatin receptor (somatostatin receptor type 5) with preferential affinity for somatostatin-28." Panetta R., Greenwood M.T., Warszynska A., Demchyshyn L.L., Day R., Niznik H.B., Srikant C.B., Patel Y.C. Mol. Pharmacol. 45:417-427(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO C-TERMINUS. Tissue: Pituitary.
[3]	"Somatostatin receptor 5 is palmitoylated by the interacting ZDHHC5 palmitoyltransferase." Kokkola T., Kruse C., Roy-Pogodzik E.M., Pekkinen J., Bauch C., Honck H.H., Hennemann H., Kreienkamp H.J. FEBS Lett. 585:2665-2670(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ZDHHC5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L04535 mRNA. Translation: AAA17029.1. U01152 mRNA. Translation: AAC09011.1. X74828 mRNA. Translation: CAA52825.1.
IPI	IPI00213662.
PIR	I57940.
UniGene	Rn.91342.
3D structure databases
ProteinModelPortal	P30938.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1489396.
STRING	10116.ENSRNOP00000025451.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P30938.
Proteomic databases
PRIDE	P30938.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:3765. rat.
Organism-specific databases
RGD	3765. Sstr5.
Phylogenomic databases
eggNOG	NOG274661.
HOGENOM	HOG000230485.
HOVERGEN	HBG106919.
InParanoid	P30938.
OrthoDB	EOG4Q84Z1.
Gene expression databases
Genevestigator	P30938.
GermOnline	ENSRNOG00000018834. Rattus norvegicus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000586. Somatstn_rcpt. IPR001184. Somatstn_rcpt_5. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00246. SOMATOSTATNR. PR00591. SOMATOSTTN5R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P30938.
ChEMBL	CHEMBL4318.

Entry information

Entry name

SSR5_RAT

Accession

Primary (citable) accession number: P30938

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program