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Protein

Somatostatin receptor type 3

Gene

Sstr3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for somatostatin-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to estradiol stimulus Source: RGD
  • cellular response to glucocorticoid stimulus Source: RGD
  • cerebellum development Source: RGD
  • forebrain development Source: RGD
  • G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
  • negative regulation of cell proliferation Source: GO_Central
  • neuropeptide signaling pathway Source: GO_Central
  • regulation of insulin secretion Source: GO_Central
  • response to starvation Source: RGD
  • somatostatin signaling pathway Source: GO_Central
  • spermatogenesis Source: RGD
  • synaptic transmission Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_306851. Peptide ligand-binding receptors.
REACT_339234. G alpha (i) signalling events.
REACT_357844. BBSome-mediated cargo-targeting to cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
Somatostatin receptor type 3
Short name:
SS-3-R
Short name:
SS3-R
Short name:
SS3R
Alternative name(s):
SSR-28
Gene namesi
Name:Sstr3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi620308. Sstr3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545ExtracellularSequence AnalysisAdd
BLAST
Transmembranei46 – 7126Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini72 – 8110CytoplasmicSequence Analysis
Transmembranei82 – 10322Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini104 – 11815ExtracellularSequence AnalysisAdd
BLAST
Transmembranei119 – 14022Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini141 – 16222CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei163 – 18220Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini183 – 20624ExtracellularSequence AnalysisAdd
BLAST
Transmembranei207 – 23226Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini233 – 26634CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei267 – 28822Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini289 – 30214ExtracellularSequence AnalysisAdd
BLAST
Transmembranei303 – 32523Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini326 – 428103CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • ciliary membrane Source: Ensembl
  • cytoplasm Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • neuron projection Source: GO_Central
  • nonmotile primary cilium Source: Ensembl
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi341 – 3411S → A: Impaired internalization after somatostatin binding. 1 Publication
Mutagenesisi346 – 3461S → A: Impaired internalization after somatostatin binding. 1 Publication
Mutagenesisi351 – 3511S → A: Impaired internalization after somatostatin binding. 1 Publication
Mutagenesisi357 – 3571T → A: Reduced basal and somatostatin-induced phosphorylation. Impaired internalization after somatostatin binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Somatostatin receptor type 3PRO_0000070126Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi18 – 181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi31 – 311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi117 ↔ 192PROSITE-ProRule annotation
Modified residuei341 – 3411Phosphoserine1 Publication
Modified residuei346 – 3461Phosphoserine1 Publication
Modified residuei351 – 3511Phosphoserine1 Publication
Modified residuei357 – 3571Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylated. Phosphorylation increases upon somatostatin binding.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP30936.

PTM databases

PhosphoSiteiP30936.

Expressioni

Tissue specificityi

Densely expressed in cerebellum and at moderate levels in the amygdala, cortex, striatum, spleen, liver and pituitary.1 Publication

Gene expression databases

GenevisibleiP30936. RN.

Interactioni

Subunit structurei

Homodimer and heterodimer with SSTR2. Heterodimerization with SSTR2 inactivates SSTR3 receptor function.1 Publication

Protein-protein interaction databases

BioGridi251061. 1 interaction.
STRINGi10116.ENSRNOP00000009612.

Structurei

3D structure databases

ProteinModelPortaliP30936.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi358 – 37316Poly-GluAdd
BLAST

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG274661.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230485.
HOVERGENiHBG106919.
InParanoidiP30936.
KOiK04219.
OMAiAGPPEKT.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP30936.
TreeFamiTF315737.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000586. Somatstn_rcpt.
IPR001856. Somatstn_rcpt_3.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00246. SOMATOSTATNR.
PR00589. SOMATOSTTN3R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30936-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVTYPSSV PTTLDPGNAS SAWPLDTSLG NASAGTSLAG LAVSGILISL
60 70 80 90 100
VYLVVCVVGL LGNSLVIYVV LRHTSSPSVT SVYILNLALA DELFMLGLPF
110 120 130 140 150
LAAQNALSYW PFGSLMCRLV MAVDGINQFT SIFCLTVMSV DRYLAVVHPT
160 170 180 190 200
RSARWRTAPV ARMVSAAVWV ASAVVVLPVV VFSGVPRGMS TCHMQWPEPA
210 220 230 240 250
AAWRTAFIIY TAALGFFGPL LVICLCYLLI VVKVRSTTRR VRAPSCQWVQ
260 270 280 290 300
APACQRRRRS ERRVTRMVVA VVALFVLCWM PFYLLNIVNV VCPLPEEPAF
310 320 330 340 350
FGLYFLVVAL PYANSCANPI LYGFLSYRFK QGFRRILLRP SRRVRSQEPG
360 370 380 390 400
SGPPEKTEEE EDEEEEERRE EEERRMQRGQ EMNGRLSQIA QPGPSGQQQR
410 420
PCTGTAKEQQ LLPQEATAGD KASTLSHL
Length:428
Mass (Da):47,151
Last modified:July 1, 1993 - v1
Checksum:iBE0AA948840A9E9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63574 mRNA. Translation: CAA45130.1.
PIRiS30508.
RefSeqiNP_598206.1. NM_133522.1.
XP_006242028.1. XM_006241966.2.
UniGeneiRn.10458.

Genome annotation databases

EnsembliENSRNOT00000009612; ENSRNOP00000009612; ENSRNOG00000007332.
GeneIDi171044.
KEGGirno:171044.
UCSCiRGD:620308. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63574 mRNA. Translation: CAA45130.1.
PIRiS30508.
RefSeqiNP_598206.1. NM_133522.1.
XP_006242028.1. XM_006241966.2.
UniGeneiRn.10458.

3D structure databases

ProteinModelPortaliP30936.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251061. 1 interaction.
STRINGi10116.ENSRNOP00000009612.

Chemistry

BindingDBiP30936.
ChEMBLiCHEMBL2096977.
GuidetoPHARMACOLOGYi357.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP30936.

Proteomic databases

PaxDbiP30936.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009612; ENSRNOP00000009612; ENSRNOG00000007332.
GeneIDi171044.
KEGGirno:171044.
UCSCiRGD:620308. rat.

Organism-specific databases

CTDi6753.
RGDi620308. Sstr3.

Phylogenomic databases

eggNOGiNOG274661.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230485.
HOVERGENiHBG106919.
InParanoidiP30936.
KOiK04219.
OMAiAGPPEKT.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP30936.
TreeFamiTF315737.

Enzyme and pathway databases

ReactomeiREACT_306851. Peptide ligand-binding receptors.
REACT_339234. G alpha (i) signalling events.
REACT_357844. BBSome-mediated cargo-targeting to cilium.

Miscellaneous databases

NextBioi621561.
PROiP30936.

Gene expression databases

GenevisibleiP30936. RN.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000586. Somatstn_rcpt.
IPR001856. Somatstn_rcpt_3.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00246. SOMATOSTATNR.
PR00589. SOMATOSTTN3R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of a somatostatin-28 receptor and comparison of its expression pattern with that of a somatostatin-14 receptor in rat brain."
    Meyerhof W., Wulfsen I., Schoenrock C., Fehr S., Richter D.
    Proc. Natl. Acad. Sci. U.S.A. 89:10267-10271(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Brain.
  2. "Phosphorylation of four amino acid residues in the carboxyl terminus of the rat somatostatin receptor subtype 3 is crucial for its desensitization and internalization."
    Roth A., Kreienkamp H.-J., Meyerhof W., Richter D.
    J. Biol. Chem. 272:23769-23774(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF SER-341; SER-346; SER-351 AND THR-357, PHOSPHORYLATION AT SER-341; SER-346; SER-351 AND THR-357.
  3. "Homo- and heterodimerization of somatostatin receptor subtypes. Inactivation of sst(3) receptor function by heterodimerization with sst(2A)."
    Pfeiffer M., Koch T., Schroder H., Klutzny M., Kirscht S., Kreienkamp H.J., Hollt V., Schulz S.
    J. Biol. Chem. 276:14027-14036(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSSR3_RAT
AccessioniPrimary (citable) accession number: P30936
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 22, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.