P30929 (L_MUMPM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Mumps virus (strain Miyahara vaccine) (MuV)

Taxonomic identifier

11171 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	2261 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the paramyxovirus L protein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2261	2261	RNA-directed RNA polymerase L		PRO_0000142730
Regions
	Domain	662 – 846	185	RdRp catalytic
	Nucleotide binding	1811 – 1820	10	ATP Potential
	Compositional bias	1046 – 1050	5	Poly-Glu

Sequences

	Sequence		Length	Mass (Da)
	P30929 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: A789F54BFC1EB5EB	FASTA	2,261	256,575
	10 20 30 40 50 60 MAGLNEILLP EVHLNSPIVR YKLFYYILHG QLPNDLEPDD LGPLANQNWK AIRAEESQVH 70 80 90 100 110 120 ARLKQIRVEL IARIPSLRWT RSQREIAILI WPRILPILQA YDLRQSMQLP TVWEKLTQST 130 140 150 160 170 180 VNLISDGLER VVLHISNQLT GKPNLFTRSR AGQDVKDYSI PSTRELSQIW FNNEWSGSVK 190 200 210 220 230 240 TWLMIKYRMR QLITNQKTGE LTDLVTIVDT RSTLCIIAPE LVALYSNEHK ALTYLTFEMV 250 260 270 280 290 300 LMVTDMLEGR LNVSSLCTAS HYLSPLKKRI EILLTLVDDL ALLMGDKVYG VVSSLESFVY 310 320 330 340 350 360 AQLQYGDPVV DIKGTFYGFI CNEILDLLTE DNIFTEEEAN KVLLDLTSQF DNLSPDLTAE 370 380 390 400 410 420 LLCIMRLWGH PTLTASQAAS KVRESMCAPK VLDFQTIMKT LAFFHAILIN GYRRSHNGIW 430 440 450 460 470 480 PPTTLHGNAP KSLIEMRHDN SELKYEYVLK NWKSISMLRI HKCFDASPDE DLSIFMKDKA 490 500 510 520 530 540 ISCPKQDWMG VFRRSLIKQR YRDANRPLPQ PFNRRLLLNF LEDDRFDPIK ELEYVTSGEY 550 560 570 580 590 600 LRDPEFCASY SLKEKEIKAT GRIFAKMTKR MRSCQVIAES LLANHAGKLM RENGVVLDQL 610 620 630 640 650 660 KLTKSLLTMN QIGIISEHSR RSTADNMTLA HSGSNKHRIN NSQFKKNKDS KHEMPDDGFE 670 680 690 700 710 720 IAACFLTTDL TKYCLNWRYQ VIIPFARTLN SMYGIPHLFE WIHLRLMRST LYVGDPFNPP 730 740 750 760 770 780 SDPTQLDLDT ALNDDIFIVS PRGGIEGLCQ KLWTMISIST IILSATEANT RVMSMVQGDN 790 800 810 820 830 840 QAIAITTRVV RSLSHSEKKE QAYKASKLFF ERLRANNHGI GHHLKEQETI LSSDFFIYSK 850 860 870 880 890 900 RVFYKGRILT QALKNVSKMC LTADILGDCS QASCSNLATT VMRLTENGVE KDLCYFLNAF 910 920 930 940 950 960 MTIRQLCYDL VFPQTKSLSQ DITNAYLNHP ILISRLCLLP SQLGGLNFLS CSRLFNRNIG 970 980 990 1000 1010 1020 DPLVSAIADV KRLIKAGCLD IWVLYNILGR RPGKGKWSTL AADPYTLNID YLVPSKTFLK 1030 1040 1050 1060 1070 1080 KHAQYTLMER SVNPMLRGVF SENAAEEEEE LAQYLLDREV VMPRVAHVIL AQSSCGRRKQ 1090 1100 1110 1120 1130 1140 IQGYLDSTRT IIRYSLEVRP LSAKKLNTVI EYNLLYLSYN LEIIEKPNIV QPFLNAINVD 1150 1160 1170 1180 1190 1200 TCSIDIARSL RKLSWATLLN GRPIEGLETP DPIELVHGCL IIGSDECEHC SSGDDKFTWF 1210 1220 1230 1240 1250 1260 FLPKGIRLDN DPASNPPIRV PYIGSKTDER RVASMAYIKG ASVSLKSALR LAGVYIWAFG 1270 1280 1290 1300 1310 1320 DTEESWQDAY ELASTRVNLT LEQLQSLTPL PTSANLVHRL DDGTTQLKFT PASSYAFSSF 1330 1340 1350 1360 1370 1380 VHISNDCQVL EIDDQVTDSN LIYQQVMITG LALIETWNNP PINFSVYETT LHLHTGSSCC 1390 1400 1410 1420 1430 1440 IRPVESCVVN PPLLPVPFIN VPQMNKFVYD PEPLSLLEME KIEDIAYQTR IGGLDQIPLL 1450 1460 1470 1480 1490 1500 EKIPLLAHLT AKQMVNSITG LDEATSIVND AVVQADYTSN WISECCYTYI DSVFVYSGWA 1510 1520 1530 1540 1550 1560 LLLELSYQMY YLRIQGIQGI LDYVYMTLRR IPGMAITGIS STISHPRILR RCINLDVIAP 1570 1580 1590 1600 1610 1620 INSPHIASLD YTKLSIDAVM WGTKQVLTNI SQGIDYEIVV PSESQLTLSD RVLNLVARKL 1630 1640 1650 1660 1670 1680 SLLAIIWANY NYPPKVKGMS PEDKCQALTT HLLQTVEYVE HIQIEKTNIR RMIIEPKLTA 1690 1700 1710 1720 1730 1740 YPSNLFYLSR KLLNAIRDSE EGQFLIASYY NSFGYLEPIL MESKIFNLSS SESASLTEFD 1750 1760 1770 1780 1790 1800 FILNLELSEA SLEKYSLPSL LMTAENMDNP FPQPPLHHVL RPLGLSSTSW YKTISVLNYI 1810 1820 1830 1840 1850 1860 SHMKISDGAH LYLAEGSGAS MSLIETFLPG EIIWYNSLFN SGENPPQRNF APLPTQFIES 1870 1880 1890 1900 1910 1920 VPYRLIQAGI AAGSGVVQSF YPLWNGNSDI TDLSTKTSVE YIIHKVGADT CALVHVDLEG 1930 1940 1950 1960 1970 1980 VPGSMNSMLE RAQVHALLIT VTVLKPGGLL ILKASWEPFN RFSFLLTILW QFSSTIRILR 1990 2000 2010 2020 2030 2040 SSYSDPNNHE VYIIATLAVD PTTSSFTTAL NRARTLNEQG FSLIPPELVS EYWRRRVEQG 2050 2060 2070 2080 2090 2100 QIIQDCIDKV ISECVRDQYL ADNNIILQAG GTPSTRKWLD LPDYLSFNEL QSEMARLITI 2110 2120 2130 2140 2150 2160 HLKEVIEILK GQSSDHDTLL FTSYNVGPLG KINTILRLIV ERILMYTVRN WCILPTQTRL 2170 2180 2190 2200 2210 2220 TLRQSIELGE FRLRDVITPM EILKLSPNRK YLKSALNQST FNHLMGETSD ILLNRSYQKR 2230 2240 2250 2260 IWKAIGCVIY CFGLLTPDVE DSERIDIDND IPDYDIHGDI I

References

[1]	"Molecular cloning and sequence analysis of the mumps virus gene encoding the L protein and the trailer sequence." Okazaki K., Tanabayashi K., Takeuchi K., Hishiyama M., Okazaki K., Yamada A. Virology 188:926-930(1992) [PubMed: 1585659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10575 Genomic RNA. Translation: BAA01432.1. AB040874 Genomic RNA. Translation: BAA94391.1.
PIR	A42548.
RefSeq	NP_054714.1. NC_002200.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1489763.
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR016269. RNA-dir_pol_paramyxovirus. IPR024352. RNA_pol_cap_MeTfrase. [Graphical view]
Pfam	PF12803. G-7-MTase. 1 hit. PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000830. RNA_pol_ParamyxoV. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_MUMPM

Accession

Primary (citable) accession number: P30929
Secondary accession number(s): Q783V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program