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Protein

Neuronal acetylcholine receptor subunit beta-4

Gene

CHRNB4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

  • acetylcholine-activated cation-selective channel activity Source: UniProtKB
  • acetylcholine binding Source: Ensembl
  • acetylcholine receptor activity Source: UniProtKB
  • drug binding Source: Ensembl
  • ligand-gated ion channel activity Source: DFLAT

GO - Biological processi

  • action potential Source: Ensembl
  • behavioral response to nicotine Source: Ensembl
  • ion transport Source: UniProtKB
  • locomotory behavior Source: Ensembl
  • positive regulation of transmission of nerve impulse Source: Ensembl
  • protein heterooligomerization Source: Ensembl
  • regulation of neurotransmitter secretion Source: UniProtKB
  • regulation of smooth muscle contraction Source: Ensembl
  • response to nicotine Source: GO_Central
  • signal transduction Source: UniProtKB
  • smooth muscle contraction Source: Ensembl
  • synaptic transmission, cholinergic Source: ProtInc
  • synaptic transmission involved in micturition Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000117971-MONOMER.
ReactomeiR-HSA-629587. Highly sodium permeable acetylcholine nicotinic receptors.
R-HSA-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-HSA-629597. Highly calcium permeable nicotinic acetylcholine receptors.
R-HSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit beta-4
Gene namesi
Name:CHRNB4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:1964. CHRNB4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 236ExtracellularSequence analysisAdd BLAST215
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Topological domaini258 – 265CytoplasmicSequence analysis8
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Topological domaini287 – 298ExtracellularSequence analysisAdd BLAST12
Transmembranei299 – 319HelicalSequence analysisAdd BLAST21
Topological domaini320 – 460CytoplasmicSequence analysisAdd BLAST141
Transmembranei461 – 481HelicalSequence analysisAdd BLAST21
Topological domaini482 – 498ExtracellularSequence analysisAdd BLAST17

GO - Cellular componenti

  • acetylcholine-gated channel complex Source: UniProtKB
  • cell junction Source: UniProtKB-KW
  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: UniProtKB
  • neuron projection Source: Ensembl
  • plasma membrane Source: Reactome
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi1143.
OpenTargetsiENSG00000117971.
PharmGKBiPA26496.

Chemistry databases

ChEMBLiCHEMBL3038459.
DrugBankiDB00514. Dextromethorphan.
DB00898. Ethanol.
DB00674. Galantamine.
DB01227. Levomethadyl Acetate.
DB00184. Nicotine.
DB01090. Pentolinium.

Polymorphism and mutation databases

BioMutaiCHRNB4.
DMDMi2506129.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000038922 – 498Neuronal acetylcholine receptor subunit beta-4Add BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi36N-linked (GlcNAc...)Sequence analysis1
Glycosylationi93N-linked (GlcNAc...)Sequence analysis1
Glycosylationi138N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi153 ↔ 167By similarity
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP30926.
PaxDbiP30926.
PRIDEiP30926.

PTM databases

iPTMnetiP30926.
PhosphoSitePlusiP30926.

Expressioni

Gene expression databases

BgeeiENSG00000117971.
CleanExiHS_CHRNB4.
ExpressionAtlasiP30926. baseline and differential.
GenevisibleiP30926. HS.

Organism-specific databases

HPAiCAB000109.

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Beta-4 subunit can be combined to alpha-2, alpha-3 or alpha-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly.1 Publication

Protein-protein interaction databases

BioGridi107565. 1 interactor.
IntActiP30926. 1 interactor.
STRINGi9606.ENSP00000261751.

Chemistry databases

BindingDBiP30926.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ASGmodel-B/D/E262-284[»]
ProteinModelPortaliP30926.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiP30926.
KOiK04815.
OMAiFMKRPGP.
OrthoDBiEOG091G0R20.
PhylomeDBiP30926.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P30926-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRAPSLVLF FLVALCGRGN CRVANAEEKL MDDLLNKTRY NNLIRPATSS
60 70 80 90 100
SQLISIKLQL SLAQLISVNE REQIMTTNVW LKQEWTDYRL TWNSSRYEGV
110 120 130 140 150
NILRIPAKRI WLPDIVLYNN ADGTYEVSVY TNLIVRSNGS VLWLPPAIYK
160 170 180 190 200
SACKIEVKYF PFDQQNCTLK FRSWTYDHTE IDMVLMTPTA SMDDFTPSGE
210 220 230 240 250
WDIVALPGRR TVNPQDPSYV DVTYDFIIKR KPLFYTINLI IPCVLTTLLA
260 270 280 290 300
ILVFYLPSDC GEKMTLCISV LLALTFFLLL ISKIVPPTSL DVPLIGKYLM
310 320 330 340 350
FTMVLVTFSI VTSVCVLNVH HRSPSTHTMA PWVKRCFLHK LPTFLFMKRP
360 370 380 390 400
GPDSSPARAF PPSKSCVTKP EATATSTSPS NFYGNSMYFV NPASAASKSP
410 420 430 440 450
AGSTPVAIPR DFWLRSSGRF RQDVQEALEG VSFIAQHMKN DDEDQSVVED
460 470 480 490
WKYVAMVVDR LFLWVFMFVC VLGTVGLFLP PLFQTHAASE GPYAAQRD
Length:498
Mass (Da):56,380
Last modified:November 1, 1997 - v2
Checksum:i0D48AB203F3FD03E
GO
Isoform 2 (identifier: P30926-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     120-217: NADGTYEVSV...GRRTVNPQDP → KSLRTGSTWL...GKDGGLGCPL
     218-498: Missing.

Show »
Length:231
Mass (Da):25,862
Checksum:i4182D0886DD041BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72 – 73EQ → DE in CAA48336 (PubMed:1330682).Curated2
Sequence conflicti121Missing in CAC34819 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04817491T → I.Corresponds to variant rs12914008dbSNPEnsembl.1
Natural variantiVAR_013241136R → W.1 PublicationCorresponds to variant rs141876090dbSNPEnsembl.1
Natural variantiVAR_013242140S → G.1 PublicationCorresponds to variant rs56218866dbSNPEnsembl.1
Natural variantiVAR_013243467M → V.1 PublicationCorresponds to variant rs61737502dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_046674120 – 217NADGT…NPQDP → KSLRTGSTWLWWWTGCSCGC SCLCASWALWGSSYRPSSRP MQLLRGPTLPSVTEGPLGCG VRGCEWPGGHFAASFWVVAD EALSKYVSIGHQPHQTSHSR GTGKDGGLGCPL in isoform 2. 1 PublicationAdd BLAST98
Alternative sequenceiVSP_046675218 – 498Missing in isoform 2. 1 PublicationAdd BLAST281

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62439 mRNA. Translation: AAB40117.1.
U48861 mRNA. Translation: AAA92123.1.
Y08416 mRNA. Translation: CAA69693.1.
AJ306454
, AJ306455, AJ306456, AJ306457, AJ306458, AJ306459 Genomic DNA. Translation: CAC34819.1.
AF306329
, AF306325, AF306326, AF306327, AF306328 Genomic DNA. Translation: AAL02062.1.
AC022748 Genomic DNA. No translation available.
AC067863 Genomic DNA. No translation available.
BC096080 mRNA. Translation: AAH96080.1.
BC096081 mRNA. Translation: AAH96081.1.
BC096083 mRNA. Translation: AAH96083.1.
BC096082 mRNA. Translation: AAH96082.1.
AF453877 Genomic DNA. Translation: AAL57840.1.
X68275 mRNA. Translation: CAA48336.1.
CCDSiCCDS10306.1. [P30926-1]
CCDS58392.1. [P30926-2]
PIRiG02421.
RefSeqiNP_000741.1. NM_000750.3. [P30926-1]
NP_001243496.1. NM_001256567.1. [P30926-2]
UniGeneiHs.624178.

Genome annotation databases

EnsembliENST00000261751; ENSP00000261751; ENSG00000117971. [P30926-1]
ENST00000412074; ENSP00000416386; ENSG00000117971. [P30926-2]
GeneIDi1143.
KEGGihsa:1143.
UCSCiuc002bed.2. human. [P30926-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62439 mRNA. Translation: AAB40117.1.
U48861 mRNA. Translation: AAA92123.1.
Y08416 mRNA. Translation: CAA69693.1.
AJ306454
, AJ306455, AJ306456, AJ306457, AJ306458, AJ306459 Genomic DNA. Translation: CAC34819.1.
AF306329
, AF306325, AF306326, AF306327, AF306328 Genomic DNA. Translation: AAL02062.1.
AC022748 Genomic DNA. No translation available.
AC067863 Genomic DNA. No translation available.
BC096080 mRNA. Translation: AAH96080.1.
BC096081 mRNA. Translation: AAH96081.1.
BC096083 mRNA. Translation: AAH96083.1.
BC096082 mRNA. Translation: AAH96082.1.
AF453877 Genomic DNA. Translation: AAL57840.1.
X68275 mRNA. Translation: CAA48336.1.
CCDSiCCDS10306.1. [P30926-1]
CCDS58392.1. [P30926-2]
PIRiG02421.
RefSeqiNP_000741.1. NM_000750.3. [P30926-1]
NP_001243496.1. NM_001256567.1. [P30926-2]
UniGeneiHs.624178.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ASGmodel-B/D/E262-284[»]
ProteinModelPortaliP30926.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107565. 1 interactor.
IntActiP30926. 1 interactor.
STRINGi9606.ENSP00000261751.

Chemistry databases

BindingDBiP30926.
ChEMBLiCHEMBL3038459.
DrugBankiDB00514. Dextromethorphan.
DB00898. Ethanol.
DB00674. Galantamine.
DB01227. Levomethadyl Acetate.
DB00184. Nicotine.
DB01090. Pentolinium.

PTM databases

iPTMnetiP30926.
PhosphoSitePlusiP30926.

Polymorphism and mutation databases

BioMutaiCHRNB4.
DMDMi2506129.

Proteomic databases

EPDiP30926.
PaxDbiP30926.
PRIDEiP30926.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261751; ENSP00000261751; ENSG00000117971. [P30926-1]
ENST00000412074; ENSP00000416386; ENSG00000117971. [P30926-2]
GeneIDi1143.
KEGGihsa:1143.
UCSCiuc002bed.2. human. [P30926-1]

Organism-specific databases

CTDi1143.
DisGeNETi1143.
GeneCardsiCHRNB4.
HGNCiHGNC:1964. CHRNB4.
HPAiCAB000109.
MIMi118509. gene.
neXtProtiNX_P30926.
OpenTargetsiENSG00000117971.
PharmGKBiPA26496.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiP30926.
KOiK04815.
OMAiFMKRPGP.
OrthoDBiEOG091G0R20.
PhylomeDBiP30926.
TreeFamiTF315605.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000117971-MONOMER.
ReactomeiR-HSA-629587. Highly sodium permeable acetylcholine nicotinic receptors.
R-HSA-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-HSA-629597. Highly calcium permeable nicotinic acetylcholine receptors.
R-HSA-6798695. Neutrophil degranulation.

Miscellaneous databases

GeneWikiiCHRNB4.
GenomeRNAii1143.
PROiP30926.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000117971.
CleanExiHS_CHRNB4.
ExpressionAtlasiP30926. baseline and differential.
GenevisibleiP30926. HS.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACHB4_HUMAN
AccessioniPrimary (citable) accession number: P30926
Secondary accession number(s): A4FTX5
, E9PHE8, Q16607, Q4VBA5, Q8WXC8, Q9BQR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 169 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.