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Protein

Elongation factor 2

Gene

fusA

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 348GTPBy similarity
Nucleotide bindingi93 – 975GTPBy similarity
Nucleotide bindingi147 – 1504GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-689-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Gene namesi
Name:fusA
Synonyms:fus
Ordered Locus Names:SSO0728
ORF Names:C10_002
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 736735Elongation factor 2PRO_0000091048Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei603 – 6031Diphthamide1 Publication

Interactioni

Protein-protein interaction databases

STRINGi273057.SSO0728.

Structurei

3D structure databases

ProteinModelPortaliP30925.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 234217tr-type GAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01559. Archaea.
COG0480. LUCA.
HOGENOMiHOG000231589.
InParanoidiP30925.
KOiK03234.
OMAiSEPMVAY.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00054_A. EF_G_EF_2_A.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00490. aEF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30925-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRYKTVEQV LSLMKDRTRV RNIGIIAHVD HGKTTTSDTL LAASGIISPK
60 70 80 90 100
VAGEALALDY LNVEQQRGIT VKAANISLYH EAEGKGYVIN LIDTPGHVDF
110 120 130 140 150
SGRVTRSLRV LDGSIVVVDA VEGIMTQTET VLRQSLEERV RPILFINKVD
160 170 180 190 200
RLVKELKLSP QEMLNRLLDI IRQVNNLIDM YGEPEFKEKW MINPQAGNVI
210 220 230 240 250
FGSAKDKWGF SLPMAQKKGI NMKNVIDAYT ASDKSKLEEL AAQAPINEAL
260 270 280 290 300
LDAAIKFVPN PIEAQKYRIP KIWKGDLDNE LAKAMLNADP NGPIVFMITD
310 320 330 340 350
MKVDPHAGLV ATGRVFSGTL RSGEELWLVN AKTSQRILQV SLYMGPTREL
360 370 380 390 400
AEEIPAGNIA AVLGLDRARS GETAISVGFS NVQGSFERLH YISEPVVTIA
410 420 430 440 450
VEPKNPKDLT KMIDALRKLS IEDPNLVVKI NEETGEYLLS GMGFLHLEVS
460 470 480 490 500
LQLLRENYGI DVVTTPPIVV YRESIRAKSQ VFEGKSPNKH NKFYLSVEPL
510 520 530 540 550
NDKTIELISN GTIREDMDSK EMAKILRDEA SWDYDEAKRI IAIDENVNVF
560 570 580 590 600
VDLTSGVQHL REVMDTVLQG FRLAMKEGPL AHEPIRGVKV ILHDAVIHED
610 620 630 640 650
PAHRGPAQIY PAVRNSIFAG FLTSRPTLLE PIQKLDIRVP ADLIGNVTAV
660 670 680 690 700
ITRKRGKILD VSQIANMSRI TAEIPVSESY DMASELRGST GGRAFWGTEF
710 720 730
SRWAPVPDSI LLDVVTKIRE RKGLPKELPK VEDFLS
Length:736
Mass (Da):81,820
Last modified:January 23, 2007 - v3
Checksum:i06A96231E6EE83E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69297 Genomic DNA. Translation: CAA49157.1.
Y18930 Genomic DNA. Translation: CAB57575.1.
AE006641 Genomic DNA. Translation: AAK41025.1.
PIRiS31809.
RefSeqiWP_009991295.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41025; AAK41025; SSO0728.
GeneIDi27427048.
KEGGisso:SSO0728.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69297 Genomic DNA. Translation: CAA49157.1.
Y18930 Genomic DNA. Translation: CAB57575.1.
AE006641 Genomic DNA. Translation: AAK41025.1.
PIRiS31809.
RefSeqiWP_009991295.1. NC_002754.1.

3D structure databases

ProteinModelPortaliP30925.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO0728.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK41025; AAK41025; SSO0728.
GeneIDi27427048.
KEGGisso:SSO0728.

Phylogenomic databases

eggNOGiarCOG01559. Archaea.
COG0480. LUCA.
HOGENOMiHOG000231589.
InParanoidiP30925.
KOiK03234.
OMAiSEPMVAY.

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-689-MONOMER.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00054_A. EF_G_EF_2_A.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00490. aEF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the gene encoding thermostable elongation factor 2 in Sulfolobus solfataricus."
    de Vendittis E., Amatruda M.R., Masullo M., Bocchini V.
    Gene 136:41-48(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 5833 / MT-4.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  4. "Molecular, functional and structural properties of an archaebacterial elongation factor 2."
    Raimo G., Masullo M., Parente A., Dello Russo A., Bocchini V.
    Biochim. Biophys. Acta 1132:127-132(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-23 AND 590-604, DIPHTHAMIDE AT HIS-603.
    Strain: DSM 5833 / MT-4.

Entry informationi

Entry nameiEF2_SULSO
AccessioniPrimary (citable) accession number: P30925
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.