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Protein

1,4-alpha-glucan-branching enzyme

Gene

SBE1

Organism
Solanum tuberosum (Potato)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei429 – 4291NucleophileBy similarity
Active sitei484 – 4841Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-EC
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: InterPro
  2. starch biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Starch biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme (EC:2.4.1.18)
Alternative name(s):
Q-enzyme
Starch-branching enzyme
Gene namesi
Name:SBE1
Synonyms:SBE
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
ProteomesiUP000011115 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. amyloplast Source: UniProtKB-SubCell
  2. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8618611,4-alpha-glucan-branching enzymePRO_0000188787Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP30924.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP30924.
KOiK00700.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P30924-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEINFKVLSK PIRGSFPSFS PKVSSGASRN KICFPSQHST GLKFGSQERS
60 70 80 90 100
WDISSTPKSR VRKDERMKHS SAISAVLTDD NSTMAPLEED VKTENIGLLN
110 120 130 140 150
LDPTLEPYLD HFRHRMKRYV DQKMLIEKYE GPLEEFAQGY LKFGFNREDG
160 170 180 190 200
CIVYREWAPA AQEDEVIGDF NGWNGSNHMM EKDQFGVWSI RIPDVDSKPV
210 220 230 240 250
IPHNSRVKFR FKHGNGVWVD RIPAWIKYAT ADATKFAAPY DGVYWDPPPS
260 270 280 290 300
ERYHFKYPRP PKPRAPRIYE AHVGMSSSEP RVNSYREFAD DVLPRIKANN
310 320 330 340 350
YNTVQLMAIM EHSYYGSFGY HVTNFFAVSS RYGNPEDLKY LIDKAHSLGL
360 370 380 390 400
QVLVDVVHSH ASNNVTDGLN GFDIGQGSQE SYFHAGERGY HKLWDSRLFN
410 420 430 440 450
YANWEVLRFL LSNLRWWLEE YNFDGFRFDG ITSMLYVHHG INMGFTGNYN
460 470 480 490 500
EYFSEATDVD AVVYLMLANN LIHKIFPDAT VIAEDVSGMP GLGRPVSEGG
510 520 530 540 550
IGFDYRLAMA IPDKWIDYLK NKNDEDWSMK EVTSSLTNRR YTEKCIAYAE
560 570 580 590 600
SHDQSIVGDK TIAFLLMDKE MYSGMSCLTD ASPVVDRGIA LHKMIHFFTM
610 620 630 640 650
ALGGEGYLNF MGNEFGHPEW IDFPREGNNW SYDKCRRQWN LADSEHLRYK
660 670 680 690 700
FMNAFDRAMN SLDEKFSFLA SGKQIVSSMD DDNKVVVFER GDLVFVFNFH
710 720 730 740 750
PKNTYEGYKV GCDLPGKYRV ALDSDAWEFG GHGRTGHDVD HFTSPEGIPG
760 770 780 790 800
VPETNFNGRQ IPSKCCLLRE HVWLITELMN ACQKLKITRQ TFVVSYYQQP
810 820 830 840 850
ISRRVTRNLK IRYLQISVTL TNACQKLKFT RQTFLVSYYQ QPILRRVTRK
860
LKDSLSTNIS T
Length:861
Mass (Da):99,084
Last modified:November 1, 1995 - v2
Checksum:iF3D519AC7CF1BEF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69805 mRNA. Translation: CAA49463.1.
PIRiS34730.
RefSeqiNP_001275183.1. NM_001288254.1.
UniGeneiStu.2100.

Genome annotation databases

GeneIDi102596498.
KEGGisot:102596498.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69805 mRNA. Translation: CAA49463.1.
PIRiS34730.
RefSeqiNP_001275183.1. NM_001288254.1.
UniGeneiStu.2100.

3D structure databases

ProteinModelPortaliP30924.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi102596498.
KEGGisot:102596498.

Phylogenomic databases

InParanoidiP30924.
KOiK00700.

Enzyme and pathway databases

UniPathwayiUPA00152.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Starch branching enzyme cDNA from Solanum tuberosum."
    Poulsen P., Kreiberg J.D.
    Plant Physiol. 102:1053-1054(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Dianella.
  2. "Cloning and expression analysis of a potato cDNA that encodes branching enzyme: evidence for co-expression of starch biosynthetic genes."
    Kossmann J., Visser R.G.F., Mueller-Roeber B., Willmitzer L., Sonnewald U.
    Mol. Gen. Genet. 230:39-44(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 279-527.
    Strain: cv. Desiree.
    Tissue: Tuber.

Entry informationi

Entry nameiGLGB_SOLTU
AccessioniPrimary (citable) accession number: P30924
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.