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P30924 (GLGB_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan-branching enzyme

EC=2.4.1.18
Alternative name(s):
Q-enzyme
Starch-branching enzyme
Gene names
Name:SBE1
Synonyms:SBE
OrganismSolanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier4113 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum

Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathway

Glycan biosynthesis; starch biosynthesis.

Subunit structure

Monomer.

Subcellular location

Plastidchloroplast. Plastidamyloplast.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8618611,4-alpha-glucan-branching enzyme
PRO_0000188787

Sites

Active site4291Nucleophile By similarity
Active site4841Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P30924 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: F3D519AC7CF1BEF2

FASTA86199,084
        10         20         30         40         50         60 
MEINFKVLSK PIRGSFPSFS PKVSSGASRN KICFPSQHST GLKFGSQERS WDISSTPKSR 

        70         80         90        100        110        120 
VRKDERMKHS SAISAVLTDD NSTMAPLEED VKTENIGLLN LDPTLEPYLD HFRHRMKRYV 

       130        140        150        160        170        180 
DQKMLIEKYE GPLEEFAQGY LKFGFNREDG CIVYREWAPA AQEDEVIGDF NGWNGSNHMM 

       190        200        210        220        230        240 
EKDQFGVWSI RIPDVDSKPV IPHNSRVKFR FKHGNGVWVD RIPAWIKYAT ADATKFAAPY 

       250        260        270        280        290        300 
DGVYWDPPPS ERYHFKYPRP PKPRAPRIYE AHVGMSSSEP RVNSYREFAD DVLPRIKANN 

       310        320        330        340        350        360 
YNTVQLMAIM EHSYYGSFGY HVTNFFAVSS RYGNPEDLKY LIDKAHSLGL QVLVDVVHSH 

       370        380        390        400        410        420 
ASNNVTDGLN GFDIGQGSQE SYFHAGERGY HKLWDSRLFN YANWEVLRFL LSNLRWWLEE 

       430        440        450        460        470        480 
YNFDGFRFDG ITSMLYVHHG INMGFTGNYN EYFSEATDVD AVVYLMLANN LIHKIFPDAT 

       490        500        510        520        530        540 
VIAEDVSGMP GLGRPVSEGG IGFDYRLAMA IPDKWIDYLK NKNDEDWSMK EVTSSLTNRR 

       550        560        570        580        590        600 
YTEKCIAYAE SHDQSIVGDK TIAFLLMDKE MYSGMSCLTD ASPVVDRGIA LHKMIHFFTM 

       610        620        630        640        650        660 
ALGGEGYLNF MGNEFGHPEW IDFPREGNNW SYDKCRRQWN LADSEHLRYK FMNAFDRAMN 

       670        680        690        700        710        720 
SLDEKFSFLA SGKQIVSSMD DDNKVVVFER GDLVFVFNFH PKNTYEGYKV GCDLPGKYRV 

       730        740        750        760        770        780 
ALDSDAWEFG GHGRTGHDVD HFTSPEGIPG VPETNFNGRQ IPSKCCLLRE HVWLITELMN 

       790        800        810        820        830        840 
ACQKLKITRQ TFVVSYYQQP ISRRVTRNLK IRYLQISVTL TNACQKLKFT RQTFLVSYYQ 

       850        860 
QPILRRVTRK LKDSLSTNIS T 

« Hide

References

[1]"Starch branching enzyme cDNA from Solanum tuberosum."
Poulsen P., Kreiberg J.D.
Plant Physiol. 102:1053-1054(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Dianella.
[2]"Cloning and expression analysis of a potato cDNA that encodes branching enzyme: evidence for co-expression of starch biosynthetic genes."
Kossmann J., Visser R.G.F., Mueller-Roeber B., Willmitzer L., Sonnewald U.
Mol. Gen. Genet. 230:39-44(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 279-527.
Strain: cv. Desiree.
Tissue: Tuber.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69805 mRNA. Translation: CAA49463.1.
PIRS34730.
RefSeqNP_001275183.1. NM_001288254.1.
UniGeneStu.2100.

3D structure databases

ProteinModelPortalP30924.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID102596498.
KEGGsot:102596498.

Phylogenomic databases

KOK00700.

Enzyme and pathway databases

UniPathwayUPA00152.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_SOLTU
AccessionPrimary (citable) accession number: P30924
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries