Reviewed, UniProtKB/Swiss-Prot P30918 (ASPG_PIG)

Names and origin

Protein names

Recommended name:
    N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
    EC=3.5.1.26
Alternative name(s):
    Glycosylasparaginase
    Aspartylglucosaminidase
      Short name=AGA
    N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase
Cleaved into the following 2 chains:
    1- Recommended name:
            Glycosylasparaginase alpha chain
    2- Recommended name:
            Glycosylasparaginase beta chain

Gene names

Name:

AGA

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	34 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins.
Catalytic activity	N(4)-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O = N-acetyl-beta-D-glucosaminylamine + L-aspartate.
Subunit structure	Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the Ntn-hydrolase family.

Ontologies

Keywords
Cellular component	Lysosome
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›17	›17	Glycosylasparaginase alpha chain		PRO_0000002337
	Chain	18 – ›34	›17	Glycosylasparaginase beta chain		PRO_0000002338
Experimental info
	Non-adjacent residues	17 – 18	2
	Non-terminal residue	34	1

Sequences

	Sequence		Length	Mass (Da)
	P30918-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: A527053E8DBA8B56	FASTA	34	3,621
	10 20 30 SXPLPLIVNT WPFKXATTIG MVVIHLKGYT AAGT

References

[1]	"Comparison of liver glycosylasparaginases from six vertebrates." Tollersrud O.-K., Aronson N.N. Jr. Biochem. J. 282:891-897(1992) [PubMed: 1554372] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Liver.

Cross-references

3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P30918.
Enzyme and pathway databases
BRENDA	3.5.1.26. 249.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ASPG_PIG

Accession

Primary (citable) accession number: P30918

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)