Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclear export protein

Gene

NS

Organism
Influenza A virus (strain A/Turkey/Bethlehem-Glilit/1492-B/1982 H1N1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear export protein
Short name:
NEP
Alternative name(s):
Non-structural protein 2
Short name:
NS2
Gene namesi
Name:NS
OrganismiInfluenza A virus (strain A/Turkey/Bethlehem-Glilit/1492-B/1982 H1N1)
Taxonomic identifieri31663 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 121121Nuclear export proteinPRO_0000079006Add
BLAST

Interactioni

Subunit structurei

Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP30915.
SMRiP30915. Positions 65-116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2110Nuclear export signalBy similarity
Motifi85 – 9410Nuclear export signalBy similarity

Sequence similaritiesi

Belongs to the influenza viruses NEP family.Curated

Family and domain databases

InterProiIPR000968. Flu_NS2.
[Graphical view]
PfamiPF00601. Flu_NS2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform NEP (identifier: P30915-1) [UniParc]FASTAAdd to basket

Also known as: NS2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSNTITSFQ DILQRMSKMQ LESSSADLNG MITQFERLKI YRDSLGESVM
60 70 80 90 100
RMGDLHSLQN RNATWRDELS QKFEEIRWLI AECRNILTRT ENSFEQITFL
110 120
QALQLLLEVE SEIRTFSFQL I
Length:121
Mass (Da):14,300
Last modified:July 1, 1993 - v1
Checksum:i28B84B8EC4793324
GO
Isoform NS1 (identifier: P30910-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry P30910.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:230
Mass (Da):26,174
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55467 Genomic RNA. Translation: ABG72674.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55467 Genomic RNA. Translation: ABG72674.1.

3D structure databases

ProteinModelPortaliP30915.
SMRiP30915. Positions 65-116.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR000968. Flu_NS2.
[Graphical view]
PfamiPF00601. Flu_NS2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Phylogenetic relationship of the nonstructural (NS) genes of influenza A viruses."
    Ludwig S., Schultz U., Mandler J., Fitch W.M., Scholtissek C.
    Virology 183:566-577(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNEP_I82A8
AccessioniPrimary (citable) accession number: P30915
Secondary accession number(s): Q0PDL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 24, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Average number present in a viral particle is estimated to be 130-200 molecules.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.