Reviewed, UniProtKB/Swiss-Prot P30907 (AL3A1_BOVIN)

Names and origin

Protein names

Recommended name:
    Aldehyde dehydrogenase, dimeric NADP-preferring
    EC=1.2.1.5
Alternative name(s):
    Aldehyde dehydrogenase family 3 member A1
      Short name=Aldehyde dehydrogenase 3
    Corneal 15.8 kDa protein
    Corneal protein 54
    BCP54
    Transparentin

Gene names

Name:	ALDH3A1
Synonyms:	ALDH3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	239 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein may play a central role in corneal transparency. It represents about 50 percent of the corneal epithelial soluble proteins. In the cornea, this enzyme may help in absorbing the damaging UV-B, as well as detoxicating the UV-induced peroxidic aldehydes. This protein preferentially oxidizes aromatic aldehyde substrates.
Catalytic activity	An aldehyde + NAD(P)+ + H2O = an acid + NAD(P)H.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular aldehyde metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aldehyde dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›239	›239	Aldehyde dehydrogenase, dimeric NADP-preferring		PRO_0000056469
Sites
	Active site	30	1	By similarity
Amino acid modifications
	Modified residue	55	1	N6-acetyllysine By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	239	1

Sequences

	Sequence		Length	Mass (Da)
	P30907-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 16BD07206287716C	FASTA	239	26,743
	10 20 30 40 50 60 NPHYVDKDRD LDIACRRIAW GKFMNSGQTC VAPDYILCDP SIQSQVVEKL KKSLKEFYGE 70 80 90 100 110 120 DAKKSRDYGR IINSRHFQRV MGLLEGQKVA YGGTGDATTR YIAPTILTDV DPESPVMQEE 130 140 150 160 170 180 VFGPVLPIMC VRSLEEAIQF ITQREKPLAL YVFSPNDKVI KKMIAETSSG GVTANDVVVH 190 200 210 220 230 ISVHSLPYGG VGDSGMGSYH GRKSFETFSH RRSCLVRPLL NEETLKARYP RARPICPDT

References

[1]	"Degenerate oligonucleotide sequence-directed cross-species PCR cloning of the BCP 54/ALDH 3 cDNA: priming from inverted repeats and formation of tandem primer arrays." Cooper D.L., Baptist E.W. PCR Methods Appl. 1:57-62(1991) [PubMed: 1842923] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cornea.
[2]	"Bovine corneal protein 54K (BCP54) is a homologue of the tumor-associated (class 3) rat aldehyde dehydrogenase (RATALD)." Cooper D.L., Baptist E.W., Enghild J.J., Isola N.R., Klintworth G.K. Gene 98:201-207(1991) [PubMed: 2016061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-140. Tissue: Cornea.
[3]	"Partial amino acid sequence determination of bovine corneal protein 54 K (BCP 54)." Cooper D.L., Baptist E.W., Enghild J.J., Lee H., Isola N.R., Klintworth G.K. Curr. Eye Res. 9:781-786(1990) [PubMed: 2276277] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S51969 mRNA. Translation: AAB24736.2. M37384 mRNA. Translation: AAA30468.1.
IPI	IPI00707707.
PIR	PS0412. T01406.
UniGene	Bt.13116
3D structure databases
HSSP	HSSP built from PDB template 1AD3 based on UniProtKB P11883.
SMR	P30907. Positions 1-230.
ModBase	Search...
Protein-protein interaction databases
STRING	P30907.
Genome annotation databases
Ensembl	ENSBTAT00000028125; ENSBTAP00000028125; ENSBTAG00000021113; Bos taurus. [Genome view]
Phylogenomic databases
HOVERGEN	P30907.
Enzyme and pathway databases
BRENDA	1.2.1.5. 251.
Family and domain databases
InterPro	IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR015590. Aldehyde_DH. IPR012394. Aldehyde_DH_NAD(P). [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF15. ALDH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL3A1_BOVIN

Accession

Primary (citable) accession number: P30907

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1997
Last modified:	October 13, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)