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P30904

- MIF_RAT

UniProt

P30904 - MIF_RAT

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Protein

Macrophage migration inhibitory factor

Gene
Mif
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity By similarity.

Catalytic activityi

Keto-phenylpyruvate = enol-phenylpyruvate.
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Proton acceptor; via imino nitrogen By similarity
Binding sitei33 – 331Substrate By similarity
Binding sitei65 – 651Substrate; via amide nitrogen By similarity
Binding sitei98 – 981Substrate By similarity

GO - Molecular functioni

  1. dopachrome isomerase activity Source: UniProtKB
  2. phenylpyruvate tautomerase activity Source: UniProtKB-EC
  3. protein complex binding Source: RGD

GO - Biological processi

  1. aging Source: RGD
  2. brain development Source: RGD
  3. brain renin-angiotensin system Source: RGD
  4. cellular response to hydrogen peroxide Source: RGD
  5. cellular response to hypoxia Source: RGD
  6. drinking behavior Source: RGD
  7. hair follicle development Source: RGD
  8. inflammatory response Source: UniProtKB-KW
  9. innate immune response Source: UniProtKB-KW
  10. metabolic process Source: GOC
  11. positive regulation of acute inflammatory response Source: RGD
  12. positive regulation of axon regeneration Source: RGD
  13. positive regulation of glucose import Source: RGD
  14. positive regulation of glycolytic process Source: RGD
  15. positive regulation of immune response Source: RGD
  16. positive regulation of potassium ion transport Source: RGD
  17. positive regulation of smooth muscle cell migration Source: RGD
  18. response to drug Source: RGD
  19. response to estradiol Source: RGD
  20. response to glucocorticoid Source: RGD
  21. response to heat Source: RGD
  22. response to hormone Source: RGD
  23. response to inorganic substance Source: RGD
  24. response to insulin Source: RGD
  25. response to lipopolysaccharide Source: RGD
  26. response to mechanical stimulus Source: RGD
  27. response to menaquinone Source: RGD
  28. response to progesterone Source: RGD
  29. response to vitamin E Source: RGD
  30. skin development Source: RGD
  31. spermatogenesis Source: RGD
  32. wound healing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Isomerase

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
Glutathione-binding 13 kDa protein
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
Gene namesi
Name:Mif
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 20

Organism-specific databases

RGDi621163. Mif.

Subcellular locationi

Secreted By similarity. Cytoplasm By similarity
Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens By similarity.

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. extracellular space Source: RGD
  3. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 115114Macrophage migration inhibitory factorPRO_0000158068Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781N6-acetyllysine; alternate By similarity
Modified residuei78 – 781N6-succinyllysine; alternate By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP30904.
PRIDEiP30904.

PTM databases

PhosphoSiteiP30904.

Expressioni

Tissue specificityi

Expressed in a wide variety of organs including brain, spleen, liver, muscle and kidney.

Gene expression databases

GenevestigatoriP30904.

Interactioni

Subunit structurei

Homotrimer. Interacts with BNIPL By similarity. Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain By similarity.

Protein-protein interaction databases

IntActiP30904. 3 interactions.
MINTiMINT-4590553.
STRINGi10116.ENSRNOP00000008608.

Structurei

Secondary structure

1
115
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86
Helixi12 – 143
Helixi19 – 3113
Helixi35 – 373
Beta strandi39 – 435
Beta strandi58 – 669
Helixi70 – 8819
Helixi92 – 943
Beta strandi95 – 1028

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FIMX-ray2.20A2-115[»]
ProteinModelPortaliP30904.
SMRiP30904. Positions 2-115.

Miscellaneous databases

EvolutionaryTraceiP30904.

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.

Phylogenomic databases

eggNOGiNOG08790.
GeneTreeiENSGT00730000111101.
HOGENOMiHOG000112325.
HOVERGENiHBG003240.
InParanoidiP30904.
KOiK07253.
OMAiKRSDFGW.
OrthoDBiEOG7GXPDN.
PhylomeDBiP30904.
TreeFamiTF313853.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.
PROSITEiPS01158. MIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30904-1 [UniParc]FASTAAdd to Basket

« Hide

MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF    50
SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY 100
DMNAANVGWN GSTFA 115
Length:115
Mass (Da):12,477
Last modified:January 23, 2007 - v4
Checksum:i8333F7B81B098445
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511S → R in AAA62644. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S73424 mRNA. Translation: AAB32392.1.
U20999 mRNA. Translation: AAA62644.1.
U62326 mRNA. Translation: AAB04024.1.
BC061545 mRNA. Translation: AAH61545.1.
PIRiS27117.
RefSeqiNP_112313.1. NM_031051.1.
UniGeneiRn.2661.

Genome annotation databases

EnsembliENSRNOT00000008608; ENSRNOP00000008608; ENSRNOG00000006589.
GeneIDi81683.
KEGGirno:81683.
UCSCiRGD:621163. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S73424 mRNA. Translation: AAB32392.1 .
U20999 mRNA. Translation: AAA62644.1 .
U62326 mRNA. Translation: AAB04024.1 .
BC061545 mRNA. Translation: AAH61545.1 .
PIRi S27117.
RefSeqi NP_112313.1. NM_031051.1.
UniGenei Rn.2661.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FIM X-ray 2.20 A 2-115 [» ]
ProteinModelPortali P30904.
SMRi P30904. Positions 2-115.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P30904. 3 interactions.
MINTi MINT-4590553.
STRINGi 10116.ENSRNOP00000008608.

PTM databases

PhosphoSitei P30904.

Proteomic databases

PaxDbi P30904.
PRIDEi P30904.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000008608 ; ENSRNOP00000008608 ; ENSRNOG00000006589 .
GeneIDi 81683.
KEGGi rno:81683.
UCSCi RGD:621163. rat.

Organism-specific databases

CTDi 4282.
RGDi 621163. Mif.

Phylogenomic databases

eggNOGi NOG08790.
GeneTreei ENSGT00730000111101.
HOGENOMi HOG000112325.
HOVERGENi HBG003240.
InParanoidi P30904.
KOi K07253.
OMAi KRSDFGW.
OrthoDBi EOG7GXPDN.
PhylomeDBi P30904.
TreeFami TF313853.

Miscellaneous databases

EvolutionaryTracei P30904.
NextBioi 615308.

Gene expression databases

Genevestigatori P30904.

Family and domain databases

InterProi IPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view ]
PANTHERi PTHR11954. PTHR11954. 1 hit.
Pfami PF01187. MIF. 1 hit.
[Graphical view ]
ProDomi PD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF55331. SSF55331. 1 hit.
PROSITEi PS01158. MIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Glutathione binding rat liver 13k protein is the homologue of the macrophage migration inhibitory factor."
    Sakai M., Nishihira J., Hibiya Y., Koyama Y., Nishi S.
    Biochem. Mol. Biol. Int. 33:439-446(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. Wen Y., Li G., Bekhor I.
    Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lens.
  3. Sleeman M.A., Huckle J.W., Robinson M., Jahoda C.A.B., Reynolds A.J., Whitehouse C.J.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: PVG/C.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  5. "Rat liver protein linking chemical and immunological detoxification systems."
    Blocki F.A., Schlievert P.M., Wackett L.P.
    Nature 360:269-270(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-26.
  6. Lubec G., Diao W., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-12 AND 79-87, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Hippocampus.
  7. "Crystal structure of the macrophage migration inhibitory factor from rat liver."
    Suzuki M., Sugimoto H., Nakagawa A., Tanaka I., Nishihira J., Sakai M.
    Nat. Struct. Biol. 3:259-266(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
    Tissue: Liver.

Entry informationi

Entry nameiMIF_RAT
AccessioniPrimary (citable) accession number: P30904
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 127 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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