Reviewed, UniProtKB/Swiss-Prot P30901 (LDHD_LACHE)

Names and origin

Protein names	Recommended name:     D-lactate dehydrogenase       Short name=D-LDH     EC=1.1.1.28 Alternative name(s):     D-specific D-2-hydroxyacid dehydrogenase
Organism	Lactobacillus helveticus
Taxonomic identifier	1587 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(R)-lactate + NAD+ = pyruvate + NADH.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 337

336

D-lactate dehydrogenase

PRO_0000075954

Sites

Active site

236

1

Active site

265

1

Active site

297

1

Proton donor

Secondary structure

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337

Helix Strand Turn

Details...

Beta strand

3 – 8

6

Turn

11 – 13

3

Helix

14 – 23

10

Beta strand

28 – 30

3

Helix

40 – 43

4

Beta strand

48 – 52

5

Helix

60 – 67

8

Turn

68 – 70

3

Beta strand

73 – 75

3

Beta strand

77 – 79

3

Helix

86 – 91

6

Helix

104 – 120

17

Helix

122 – 130

9

Helix

144 – 146

3

Beta strand

147 – 152

6

Helix

156 – 167

12

Beta strand

171 – 175

5

Helix

183 – 186

4

Helix

193 – 196

4

Turn

197 – 199

3

Beta strand

201 – 205

5

Turn

211 – 215

5

Helix

219 – 222

4

Beta strand

229 – 233

5

Helix

237 – 239

3

Helix

242 – 250

9

Beta strand

253 – 260

8

Helix

265 – 268

4

Helix

281 – 288

8

Beta strand

292 – 294

3

Helix

303 – 322

20

Beta strand

327 – 329

3

Turn

333 – 335

3

Sequences

	Sequence		Length	Mass (Da)
	P30901-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: D0561DD82C03B3C4	FASTA	337	37,779
	10 20 30 40 50 60 MTKVFAYAIR KDEEPFLNEW KEAHKDIDVD YTDKLLTPET AKLAKGADGV VVYQQLDYTA 70 80 90 100 110 120 DTLQALADAG VTKMSLRNVG VDNIDMDKAK ELGFQITNVP VYSPNAIAEH AAIQAARVLR 130 140 150 160 170 180 QDKRMDEKMA KRDLRWAPTI GREVRDQVVG VVGTGHIGQV FMRIMEGFGA KVIAYDIFKN 190 200 210 220 230 240 PELEKKGYYV DSLDDLYKQA DVISLHVPDV PANVHMINDK SIAEMKDGVV IVNCSRGRLV 250 260 270 280 290 300 DTDAVIRGLD SGKIFGFVMD TYEDEVGVFN KDWEGKEFPD KRLADLIDRP NVLVTPHTAF 310 320 330 YTTHAVRNMV VKAFNNNLKL INGEKPDSPV ALNKNKF

References

[1]	"Cloning and overexpression of Lactobacillus helveticus D-lactate dehydrogenase gene in Escherichia coli." Kochhar S., Hottinger H., Chuard N., Taylor P.G., Atkinson T., Scawen M.D., Nicholls D.J. Eur. J. Biochem. 208:799-805(1992) [PubMed: 1396685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-36. Strain: ATCC 15009 / DSM 20075 / IFO 15019 / JCM 1120 / Lh12.
[2]	"Cloning, characterization and insertional inactivation of the Lactobacillus helveticus D(-) lactate dehydrogenase gene." Bhowmik T.K., Steele J.L. Appl. Microbiol. Biotechnol. 41:432-439(1994) [PubMed: 7765104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CNRZ 32.
[3]	Bernard N., Delcour J., Alvarez A., Cortes A., Willis C., Holbrook J.J. Submitted (OCT-1995) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

X66723 Genomic DNA. Translation: CAA47255.1.
U07604 Genomic DNA. Translation: AAA20464.1.

PIR

S29296.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DLD	X-ray	2.70	A/B	2-336	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

1.1.1.28. 39238.

Family and domain databases

InterPro

IPR006139. D-isomer_2_OHA_DH.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

Pfam

PF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]

PROSITE

PS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LDHD_LACHE

Accession

Primary (citable) accession number: P30901

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)