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Protein

D-lactate dehydrogenase

Gene
N/A
Organism
Lactobacillus helveticus (Lactobacillus suntoryeus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(R)-lactate + NAD+ = pyruvate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei176 – 1761NAD1 Publication
Binding sitei213 – 2131NAD1 Publication
Active sitei236 – 2361By similarity
Binding sitei260 – 2601NAD1 Publication
Active sitei265 – 2651By similarity
Active sitei297 – 2971Proton donorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi156 – 1572NAD1 Publication
Nucleotide bindingi207 – 2082NAD1 Publication
Nucleotide bindingi234 – 2363NAD1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKP30901.

Names & Taxonomyi

Protein namesi
Recommended name:
D-lactate dehydrogenase (EC:1.1.1.28)
Short name:
D-LDH
Alternative name(s):
D-specific 2-hydroxyacid dehydrogenase
OrganismiLactobacillus helveticus (Lactobacillus suntoryeus)
Taxonomic identifieri1587 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 337336D-lactate dehydrogenasePRO_0000075954Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi405566.lhv_0064.

Structurei

Secondary structure

1
337
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Turni11 – 133Combined sources
Helixi14 – 2310Combined sources
Beta strandi28 – 303Combined sources
Helixi40 – 434Combined sources
Beta strandi48 – 525Combined sources
Helixi60 – 678Combined sources
Turni68 – 703Combined sources
Beta strandi73 – 753Combined sources
Beta strandi77 – 793Combined sources
Helixi86 – 916Combined sources
Helixi104 – 12017Combined sources
Helixi122 – 1309Combined sources
Helixi144 – 1463Combined sources
Beta strandi147 – 1526Combined sources
Helixi156 – 16712Combined sources
Beta strandi171 – 1755Combined sources
Helixi183 – 1864Combined sources
Helixi193 – 1964Combined sources
Turni197 – 1993Combined sources
Beta strandi201 – 2055Combined sources
Turni211 – 2155Combined sources
Helixi219 – 2224Combined sources
Beta strandi229 – 2335Combined sources
Helixi237 – 2393Combined sources
Helixi242 – 2509Combined sources
Beta strandi253 – 2608Combined sources
Helixi265 – 2684Combined sources
Helixi281 – 2888Combined sources
Beta strandi292 – 2943Combined sources
Helixi303 – 32220Combined sources
Beta strandi327 – 3293Combined sources
Turni333 – 3353Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DLDX-ray2.70A/B1-337[»]
ProteinModelPortaliP30901.
SMRiP30901. Positions 1-337.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30901.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C5I. Bacteria.
COG1052. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30901-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKVFAYAIR KDEEPFLNEW KEAHKDIDVD YTDKLLTPET AKLAKGADGV
60 70 80 90 100
VVYQQLDYTA DTLQALADAG VTKMSLRNVG VDNIDMDKAK ELGFQITNVP
110 120 130 140 150
VYSPNAIAEH AAIQAARVLR QDKRMDEKMA KRDLRWAPTI GREVRDQVVG
160 170 180 190 200
VVGTGHIGQV FMRIMEGFGA KVIAYDIFKN PELEKKGYYV DSLDDLYKQA
210 220 230 240 250
DVISLHVPDV PANVHMINDK SIAEMKDGVV IVNCSRGRLV DTDAVIRGLD
260 270 280 290 300
SGKIFGFVMD TYEDEVGVFN KDWEGKEFPD KRLADLIDRP NVLVTPHTAF
310 320 330
YTTHAVRNMV VKAFNNNLKL INGEKPDSPV ALNKNKF
Length:337
Mass (Da):37,779
Last modified:January 23, 2007 - v2
Checksum:iD0561DD82C03B3C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66723 Genomic DNA. Translation: CAA47255.1.
U07604 Genomic DNA. Translation: AAA20464.1.
PIRiS29296.
RefSeqiWP_003628108.1. NZ_LSVJ01000209.1.

Genome annotation databases

GeneIDi16795165.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66723 Genomic DNA. Translation: CAA47255.1.
U07604 Genomic DNA. Translation: AAA20464.1.
PIRiS29296.
RefSeqiWP_003628108.1. NZ_LSVJ01000209.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DLDX-ray2.70A/B1-337[»]
ProteinModelPortaliP30901.
SMRiP30901. Positions 1-337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405566.lhv_0064.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi16795165.

Phylogenomic databases

eggNOGiENOG4105C5I. Bacteria.
COG1052. LUCA.

Enzyme and pathway databases

SABIO-RKP30901.

Miscellaneous databases

EvolutionaryTraceiP30901.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and overexpression of Lactobacillus helveticus D-lactate dehydrogenase gene in Escherichia coli."
    Kochhar S., Hottinger H., Chuard N., Taylor P.G., Atkinson T., Scawen M.D., Nicholls D.J.
    Eur. J. Biochem. 208:799-805(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-36.
    Strain: ATCC 15009 / DSM 20075 / JCM 1120 / NBRC 15019 / NCIMB 11971 / Lh12.
  2. "Cloning, characterization and insertional inactivation of the Lactobacillus helveticus D(-) lactate dehydrogenase gene."
    Bhowmik T.K., Steele J.L.
    Appl. Microbiol. Biotechnol. 41:432-439(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CNRZ 32.
  3. Bernard N., Delcour J., Alvarez A., Cortes A., Willis C., Holbrook J.J.
    Submitted (OCT-1995) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).

Entry informationi

Entry nameiLDHD_LACHE
AccessioniPrimary (citable) accession number: P30901
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.