Reviewed, UniProtKB/Swiss-Prot P30897 (BLAC_PROMI)

Names and origin

Protein names

Recommended name:
    Beta-lactamase
    EC=3.5.2.6
Alternative name(s):
    Carbenicillinase

Gene names

Name:

blaP

Organism

Proteus mirabilis

Taxonomic identifier

584 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes carbenicillin.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 298	270	Beta-lactamase		PRO_0000017007
Regions
	Region	237 – 239	3	Substrate binding By similarity
Sites
	Active site	72	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Disulfide bond	79 ↔ 126		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30897-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: A3710003B728BD5F	FASTA	298	32,765
	10 20 30 40 50 60 MNVRQHKASF FSVVITFLCL TLSLNANATD SVLEAVTNAE TELGARIGLA AHDLETGKRW 70 80 90 100 110 120 EHKSNERFPL SSTFKTLACA NVLQRVDLGK ERIDRVVRFS ESNLVTYSPV TEKHVGKKGM 130 140 150 160 170 180 SLAELCQATL STSDNSAANF ILQAIGGPKA LTKFLRSIGD DTTRLDRWEP ELNEAVPGDK 190 200 210 220 230 240 RDTTTPIAMV TTLEKLLIDE TLSIKSRQQL ESWLKGNEVG DALFRKGVPS DWIVADRTGA 250 260 270 280 290 GGYGSRAITA VMWPPNRKPI VAALYITETD ASFEERNAVI AKIGEQIAKT VLMENSRN

References

[1]

"Nucleotide sequence and characterization of a carbenicillin-hydrolyzing penicillinase gene from Proteus mirabilis." Sakurai Y., Tsukamoto K., Sawai T. J. Bacteriol. 173:7038-7041(1991) [PubMed: 1840585] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 29-37. Strain: GN79.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13209 Genomic DNA. Translation: BAA02496.1.
PIR	A41381.
3D structure databases
HSSP	HSSP built from PDB template 1JTG based on UniProtKB P00810.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 639.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_PROMI

Accession

Primary (citable) accession number: P30897

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)