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P30896

- BLA3_KLEPN

UniProt

P30896 - BLA3_KLEPN

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Protein

Beta-lactamase SHV-3

Gene

bla

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme hydrolyzes cefotaxime, ceftazidime and other broad spectrum cephalosporins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei164 – 1641Proton acceptorBy similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase SHV-3 (EC:3.5.2.6)
Gene namesi
Name:bla
Synonyms:shv3
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 286265Beta-lactamase SHV-3PRO_0000016982Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi73 ↔ 119By similarity

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
286
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi25 – 3612
Beta strandi38 – 469
Turni47 – 493
Beta strandi52 – 576
Helixi65 – 673
Helixi68 – 8114
Helixi95 – 973
Helixi105 – 1084
Turni109 – 1113
Helixi115 – 12511
Helixi128 – 13710
Helixi140 – 15011
Helixi164 – 1663
Helixi179 – 19113
Beta strandi192 – 1954
Helixi197 – 20812
Beta strandi211 – 2133
Helixi214 – 2185
Beta strandi226 – 2349
Turni235 – 2373
Beta strandi238 – 24710
Beta strandi253 – 2619
Helixi266 – 28217

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N9BX-ray0.90A22-286[»]
ProteinModelPortaliP30896.
SMRiP30896. Positions 22-286.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30896.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni230 – 2323Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30896 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG
60 70 80 90 100
RTLTAWRADE RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD
110 120 130 140 150
YSPVSEKHLA DGMTVGELCA AAITMSDNSA ANLLLATVGG PAGLTAFLRQ
160 170 180 190 200
IGDNVTRLDR WETELNEALP GDARDTTTPA SMAATLRKLL TSQRLSARSQ
210 220 230 240 250
LQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGASERGARG IVALLGPNNK
260 270 280
AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR
Length:286
Mass (Da):31,211
Last modified:July 1, 1993 - v1
Checksum:i82715D986508F50D
GO

Sequence databases

PIRiA37200.

Cross-referencesi

Sequence databases

PIRi A37200.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1N9B X-ray 0.90 A 22-286 [» ]
ProteinModelPortali P30896.
SMRi P30896. Positions 22-286.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P30896.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of the gene encoding SHV-3 beta-lactamase responsible for transferable cefotaxime resistance in clinical isolates of Klebsiella pneumoniae."
    Nicolas M.H., Jarlier V., Honore N., Philippon A., Cole S.T.
    Antimicrob. Agents Chemother. 33:2096-2100(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiBLA3_KLEPN
AccessioniPrimary (citable) accession number: P30896
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 1, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3