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Protein

Outer capsid glycoprotein VP7

Gene
N/A
Organism
Rotavirus B (isolate Rat/United States/IDIR/1984) (RV-B) (Rotavirus B (isolate infectious diarrhea of infant rats))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors (By similarity).By similarity

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid glycoprotein VP7
OrganismiRotavirus B (isolate Rat/United States/IDIR/1984) (RV-B) (Rotavirus B (isolate infectious diarrhea of infant rats))
Taxonomic identifieri28877 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Rattus norvegicus (Rat) [TaxID: 10116]

Subcellular locationi

Virion Curated. Host rough endoplasmic reticulum membrane Curated; Single-pass membrane protein Curated; Lumenal side Curated
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2317HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell rough endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. T=13 icosahedral viral capsid Source: UniProtKB-KW
  4. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Host membrane, Membrane, Outer capsid protein, T=13 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246Outer capsid glycoprotein VP7PRO_0000041132Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi43 – 431N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi129 – 1291N-linked (GlcNAc...); by hostSequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homotrimer; in the presence of calcium (By similarity). Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer (By similarity).By similarity

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 84Poly-Leu

Sequence similaritiesi

Belongs to the rotavirus VP7 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008818. Rotavirus_VP7.
[Graphical view]
PfamiPF05868. Rotavirus_VP7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30889-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTMLLLLVV AALANGQLTI LPHEESQICF LQPDNPGFDF DGNFTNIFRD
60 70 80 90 100
YASVKISSFT YKAQDADIVE ILNVDRDRSC TILAIYIADS TLDFNTFLQS
110 120 130 140 150
ENECVKYAAS KKHYIKLPRD REYFALAKNL SFCPLNDDLI GIYCDTQLET
160 170 180 190 200
TYFSVARSSN YDVTDIPEFT ELGYVFHSND HFYICERKSE GNWIDYQLFY
210 220 230 240
QNDAPLGTVS QRVNWGNVWS NVKTVAQMVY KILDIFFGKR NIEPRA
Length:246
Mass (Da):28,319
Last modified:June 30, 1993 - v1
Checksum:iBF28CC5BF9A169A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00911 Genomic RNA. Translation: BAA00757.1.
PIRiJQ1311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00911 Genomic RNA. Translation: BAA00757.1.
PIRiJQ1311.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR008818. Rotavirus_VP7.
[Graphical view]
PfamiPF05868. Rotavirus_VP7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparison of group B rotavirus genes 9 and 11."
    Petric M., Mayur K., Vonderfecht S., Eiden J.J.
    J. Gen. Virol. 72:2801-2804(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP7_ROTGI
AccessioniPrimary (citable) accession number: P30889
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 1993
Last sequence update: June 30, 1993
Last modified: January 6, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.