Reviewed, UniProtKB/Swiss-Prot P30877 (AGAL_SALTY)

Names and origin

Protein names

Recommended name:
    Alpha-galactosidase
    EC=3.2.1.22
Alternative name(s):
    Melibiase

Gene names

Name:	melA
Ordered Locus Names:	STM4298

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.
Cofactor	Binds 1 manganese ion per subunit By similarity. Binds 1 NAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 4 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	Manganese Metal-binding NAD
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-galactosidase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	Alpha-galactosidase		PRO_0000169854
Regions
	Nucleotide binding	5 – 71	67	NAD By similarity
Sites
	Active site	174	1	Proton donor By similarity
	Metal binding	173	1	Manganese By similarity
	Metal binding	203	1	Manganese By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	287	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30877-1 [UniParc]. Last modified December 19, 2001. Version 2. Checksum: 5106CDC87E3334DF	FASTA	451	50,735
	10 20 30 40 50 60 MMTAPKITFI GAGSTIFVKN ILGDVFHREA LKSAHVALMD IDETRLEESH IVVRKLMDSA 70 80 90 100 110 120 GASGRITCHT NQKAALQDAD FVVVAFQIGG YEPCTVTDFE VCKRHGLEQT IADTLGPGGI 130 140 150 160 170 180 MRALRTIPHL WRICEDMTEV CPKATMLNYV NPMAMNTWAM YARYPHIKQV GLCHSVQGTA 190 200 210 220 230 240 EELARDLNID PTSLRYRCAG INHMAFYLEL ERKTADGTYV NLYPELLAAY DAGQAPKPNI 250 260 270 280 290 300 HGNERCQNIV RYEMFKKLGY FVTESSEHFA EYTPWFIKPG REDLIARYKV PLDEYPKRCV 310 320 330 340 350 360 EQLANWHKEL EEYKTAERID IKPSREYAST IMNALWTGEP SVIYGNVRNE GLIDNLPQGS 370 380 390 400 410 420 CVEVACLVDA NGIQPTKVGT IPSHLAAMMQ TNINVQTLLT EAILTENRDR VYHAAMMDPH 430 440 450 TAAVLGIEEI YALVDDLIAA HGDWLPAWLR R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[2]	"Cloning and sequencing of the melB gene encoding the melibiose permease of Salmonella typhimurium LT2." Mizushima K., Awakihara S., Kuroda M., Ishikawa T., Tsuda M., Tsuchiya T. Mol. Gen. Genet. 234:74-80(1992) [PubMed: 1495487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 409-451. Strain: LT2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006468 Genomic DNA. Translation: AAL23122.1. X62101 Genomic DNA. Translation: CAA44010.1.
PIR	S42852.
RefSeq	NP_463163.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH4. Glycoside Hydrolase Family 4.
Proteomic databases
PRIDE	P30877.
Genome annotation databases
GeneID	1255824.
GenomeReviews	Gene locus STM4298 in contig AE006468_GR.
KEGG	stm:STM4298.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P30877.
OMA	MAINTWA.
Enzyme and pathway databases
BioCyc	STYP99287:STM4298-MON.
BRENDA	3.2.1.22. 2.
Family and domain databases
InterPro	IPR019802. GlycHydrolase_4_CS. IPR001088. Glyco_hydro_4. IPR015955. Lactate_DH/Glyco_Ohase_4_C. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit.
Pfam	PF02056. Glyco_hydro_4. 1 hit. [Graphical view]
PRINTS	PR00732. GLHYDRLASE4.
ProDom	PD006892. Glyco_hydro_4. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01324. GLYCOSYL_HYDROL_F4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGAL_SALTY

Accession

Primary (citable) accession number: P30877

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	December 19, 2001
Last modified:	November 3, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)