Reviewed,
UniProtKB/Swiss-Prot P30876 (RPB2_HUMAN)
Last modified
November 24, 2009.
Version 95.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: DNA-directed RNA polymerase II subunit RPB2 EC=2.7.7.6 Alternative name(s): DNA-directed RNA polymerase II subunit B RNA polymerase II subunit B2 RNA polymerase II subunit 2 DNA-directed RNA polymerase II 140 kDa polypeptide | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Complete proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1174 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template By similarity. |
| Catalytic activity | Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). |
| Subunit structure | Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. |
| Subcellular location | |
| Miscellaneous | The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits By similarity. |
| Sequence similarities | Belongs to the RNA polymerase beta chain family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1174 | 1174 | DNA-directed RNA polymerase II subunit RPB2 | PRO_0000048085 | |||||
Regions | |||||||||
| Zinc finger | 1119 – 1140 | 22 | C4-type | ||||||
Sites | |||||||||
| Metal binding | 792 | 1 | Magnesium; shared with RPB1 By similarity | ||||||
| Metal binding | 1119 | 1 | Zinc By similarity | ||||||
| Metal binding | 1122 | 1 | Zinc By similarity | ||||||
| Metal binding | 1137 | 1 | Zinc By similarity | ||||||
| Metal binding | 1140 | 1 | Zinc By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Primary structure of the second largest subunit of human RNA polymerase II (or B)." Acker J., Wintzerith M., Vigneron M., Kedinger C. J. Mol. Biol. 226:1295-1299(1992) [PubMed: 1518060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin. |
| [5] | "Large-scale concatenation cDNA sequencing." Yu W., Andersson B., Worley K.C., Muzny D.M., Ding Y., Liu W., Ricafrente J.Y., Wentland M.A., Lennon G., Gibbs R.A. Genome Res. 7:353-358(1997) [PubMed: 9110174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 699-1174. Tissue: Brain. |
| [6] | "Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes." Kershnar E., Wu S.-Y., Chiang C.-M. J. Biol. Chem. 273:34444-34453(1998) [PubMed: 9852112] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RNA POLYMERASE II CORE-COMPLEX, SUBCELLULAR LOCATION. |
| [7] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X63563 mRNA. Translation: CAA45124.1. AK289823 mRNA. Translation: BAF82512.1. CH471057 Genomic DNA. Translation: EAX05519.1. BC023503 mRNA. Translation: AAH23503.2. AF055028 mRNA. Translation: AAC09367.1. | |
| IPI | IPI00027808. |
| PIR | S28976. |
| RefSeq | NP_000929.1. |
| UniGene | Hs.602757 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P30876. 10 interactions. |
| STRING | P30876. |
PTM databases | |
| PhosphoSite | P30876. |
Proteomic databases | |
| PeptideAtlas | P30876. |
| PRIDE | P30876. |
Genome annotation databases | |
| Ensembl | ENST00000314595; ENSP00000312735; ENSG00000047315; Homo sapiens. [Genome view] ENST00000381227; ENSP00000370625; ENSG00000047315; Homo sapiens. [Genome view] |
| GeneID | 5431. |
| KEGG | hsa:5431. |
| UCSC | uc003hcl.1. human. |
Organism-specific databases | |
| CTD | 5431. |
| GeneCards | GC04P057538. |
| H-InvDB | HIX0004241. |
| HGNC | HGNC:9188. POLR2B. |
| MIM | 180661. gene. |
| PharmGKB | PA33508. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | P30876. |
| HOVERGEN | P30876. |
| OMA | FGPTYYQ |
| OrthoDB | EOG9B5RQP |
Enzyme and pathway databases | |
| BRENDA | 2.7.7.6. 247. |
| Reactome | REACT_12472. Regulatory RNA pathways. REACT_125. Processing of Capped Intron-Containing Pre-mRNA. REACT_1675. mRNA Processing. REACT_1788. Transcription. REACT_1892. Elongation arrest and recovery. REACT_216. DNA Repair. REACT_6143. Pausing and recovery of Tat-mediated HIV-1 elongation. REACT_6167. Influenza Infection. REACT_6185. HIV Infection. REACT_6244. Pausing and recovery of HIV-1 elongation. REACT_6259. HIV-1 elongation arrest and recovery. REACT_6344. Tat-mediated HIV-1 elongation arrest and recovery. REACT_71. Gene Expression. REACT_769. Pausing and recovery of elongation. |
Gene expression databases | |
| ArrayExpress | P30876. |
| Bgee | P30876. |
| CleanEx | HS_POLR2B. |
| Genevestigator | P30876. |
| GermOnline | ENSG00000047315. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007646. RNA_pol_Rpb2_4. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. [Graphical view] |
| PANTHER | PTHR20856. RNA_pol_I_sub2. 1 hit. |
| Pfam | PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF04566. RNA_pol_Rpb2_4. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view] |
| PROSITE | PS01166. RNA_POL_BETA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 21013. |
| SOURCE | Search... |
Entry information
| Entry name | RPB2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P30876 Secondary accession number(s): A8K1A8, Q8IZ61 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 4 Human chromosome 4: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


