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Protein

Somatostatin receptor type 2

Gene

Sstr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Receptor for somatostatin-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. Inhibits calcium entry by suppressing voltage-dependent calcium channels. Acts as the functionally dominant somatostatin receptor in pancreatic alpha- and beta-cells where it mediates the inhibitory effect of somatostatin-14 on hormone secretion. Inhibits cell growth through enhancement of MAPK1 and MAPK2 phosphorylation and subsequent up-regulation of CDKN1B. Stimulates neuronal migration and axon outgrowth and may participate in neuron development and maturation during brain development. Mediates negative regulation of insulin receptor signaling through PTPN6. Inactivates SSTR3 receptor function following heterodimerization.2 Publications

GO - Molecular functioni

  • neuropeptide binding Source: GO_Central
  • PDZ domain binding Source: MGI
  • somatostatin receptor activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-375276 Peptide ligand-binding receptors
R-MMU-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Somatostatin receptor type 2
Short name:
SS-2-R
Short name:
SS2-R
Short name:
SS2R
Alternative name(s):
SRIF-1
Gene namesi
Name:Sstr2
Synonyms:Smstr2, Sst2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98328 Sstr2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 43ExtracellularSequence analysisAdd BLAST43
Transmembranei44 – 67Helical; Name=1Sequence analysisAdd BLAST24
Topological domaini68 – 78CytoplasmicSequence analysisAdd BLAST11
Transmembranei79 – 103Helical; Name=2Sequence analysisAdd BLAST25
Topological domaini104 – 118ExtracellularSequence analysisAdd BLAST15
Transmembranei119 – 138Helical; Name=3Sequence analysisAdd BLAST20
Topological domaini139 – 161CytoplasmicSequence analysisAdd BLAST23
Transmembranei162 – 181Helical; Name=4Sequence analysisAdd BLAST20
Topological domaini182 – 207ExtracellularSequence analysisAdd BLAST26
Transmembranei208 – 229Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini230 – 253CytoplasmicSequence analysisAdd BLAST24
Transmembranei254 – 278Helical; Name=6Sequence analysisAdd BLAST25
Topological domaini279 – 288ExtracellularSequence analysis10
Transmembranei289 – 303Helical; Name=7Sequence analysisAdd BLAST15
Topological domaini304 – 369CytoplasmicSequence analysisAdd BLAST66

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3207
GuidetoPHARMACOLOGYi356

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000701211 – 369Somatostatin receptor type 2Add BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi9N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi22N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi29N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi32N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi115 ↔ 193PROSITE-ProRule annotation
Lipidationi328S-palmitoyl cysteineSequence analysis1
Modified residuei341PhosphoserineBy similarity1
Modified residuei343PhosphoserineBy similarity1
Modified residuei348PhosphoserineBy similarity1
Modified residuei353PhosphothreonineBy similarity1
Modified residuei354PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated on serine and threonine residues in response to agonist stimulation, leading to receptor desensitization and rapid internalization. Phosphorylated to a greater extent on serine than threonine residues. Threonine phosphorylation is required for arrestin binding and receptor endocytosis but is not necessary for desensitization (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP30875
PRIDEiP30875

PTM databases

iPTMnetiP30875
PhosphoSitePlusiP30875

Expressioni

Tissue specificityi

Cerebrum and kidney.

Gene expression databases

BgeeiENSMUSG00000047904
CleanExiMM_SSTR2
GenevisibleiP30875 MM

Interactioni

Subunit structurei

Homodimer and heterodimer with SSTR3 and SSTR5. Heterodimerization with SSTR3 inactivates SSTR3 receptor function. Heterodimerization with SSTR5 is enhanced by agonist stimulation of SSTR2 and increases SSTR2 cell growth inhibition activity. Following agonist stimulation, homodimers dissociate into monomers which is required for receptor internalization. Interacts with beta-arrestin; this interaction is necessary for receptor internalization and is destabilized by heterodimerization with SSTR5 which results in increased recycling of SSTR2 to the cell surface. Interacts (via C-terminus) with SHANK1 (via PDZ domain) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP30875, 2 interactors
MINTiP30875
STRINGi10090.ENSMUSP00000068578

Chemistry databases

BindingDBiP30875

Structurei

3D structure databases

ProteinModelPortaliP30875
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118797
HOGENOMiHOG000230485
HOVERGENiHBG106919
InParanoidiP30875
KOiK04218
OMAiCGIVWPE
OrthoDBiEOG091G0HEN
PhylomeDBiP30875
TreeFamiTF315737

Family and domain databases

CDDicd15971 7tmA_SSTR2, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR000586 Somatstn_rcpt
IPR002074 Somatstn_rcpt_2
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00246 SOMATOSTATNR
PR00588 SOMATOSTTN2R
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P30875-1) [UniParc]FASTAAdd to basket
Also known as: SS2RA

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEMSSEQLNG SQVWVSSPFD LNGSLGPSNG SNQTEPYYDM TSNAVLTFIY
60 70 80 90 100
FVVCVVGLCG NTLVIYVILR YAKMKTITNI YILNLAIADE LFMLGLPFLA
110 120 130 140 150
MQVALVHWPF GKAICRVVMT VDGINQFTSI FCLTVMSIDR YLAVVHPIKS
160 170 180 190 200
AKWRRPRTAK MINVAVWCVS LLVILPIMIY AGLRSNQWGR SSCTINWPGE
210 220 230 240 250
SGAWYTGFII YAFILGFLVP LTIICLCYLF IIIKVKSSGI RVGSSKRKKS
260 270 280 290 300
EKKVTRMVSI VVAVFIFCWL PFYIFNVSSV SVAISPTPAL KGMFDFVVIL
310 320 330 340 350
TYANSCANPI LYAFLSDNFK KSFQNVLCLV KVSGTEDGER SDSKQDKSRL
360
NETTETQRTL LNGDLQTSI
Length:369
Mass (Da):41,222
Last modified:July 1, 1993 - v1
Checksum:iA78845AF74823039
GO
Isoform B (identifier: P30875-2) [UniParc]FASTAAdd to basket
Also known as: SS2RB

The sequence of this isoform differs from the canonical sequence as follows:
     332-369: VSGTEDGERSDSKQDKSRLNETTETQRTLLNGDLQTSI → ADNSQSGAEDIIAWV

Show »
Length:346
Mass (Da):38,587
Checksum:iD7A20AEC1371C400
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti179I → L in CAA48766 (PubMed:1397330).Curated1
Sequence conflicti305S → T in CAA48766 (PubMed:1397330).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001923332 – 369VSGTE…LQTSI → ADNSQSGAEDIIAWV in isoform B. CuratedAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81832 Genomic DNA Translation: AAA58256.1
X68951 mRNA Translation: CAA48766.1
AF008914 Genomic DNA Translation: AAD01419.1
AF008914 Genomic DNA Translation: AAD01420.1
S71756 mRNA Translation: AAP20804.1
CCDSiCCDS25598.1 [P30875-2]
CCDS59571.1 [P30875-1]
PIRiD41795
S29248
RefSeqiNP_001036071.1, NM_001042606.2 [P30875-1]
NP_033243.2, NM_009217.4 [P30875-2]

Genome annotation databases

EnsembliENSMUST00000067591; ENSMUSP00000068578; ENSMUSG00000047904 [P30875-2]
ENSMUST00000106630; ENSMUSP00000102241; ENSMUSG00000047904 [P30875-2]
ENSMUST00000146390; ENSMUSP00000138101; ENSMUSG00000047904 [P30875-1]
GeneIDi20606
KEGGimmu:20606
UCSCiuc007mep.2 mouse [P30875-2]
uc011yha.2 mouse [P30875-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSSR2_MOUSE
AccessioniPrimary (citable) accession number: P30875
Secondary accession number(s): P30934, Q91Y73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: February 28, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health