P30874 (SSR2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Somatostatin receptor type 2
Short name=SS-2-R
Short name=SS2-R
Short name=SS2R

Alternative name(s):
SRIF-1

Gene names

Name:

SSTR2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels.
Subunit structure	The C-terminus interacts with SHANK1 PDZ domain. Ref.9
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Cerebrum and kidney. In lesser amounts in jejunum, colon and liver.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Traceable author statement PubMed 7914078. Source: ProtInc adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara cellular response to estradiol stimulus Inferred from electronic annotation. Source: Compara cellular response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara cerebellum development Inferred from electronic annotation. Source: Compara digestion Traceable author statement Ref.1. Source: ProtInc forebrain development Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Traceable author statement PubMed 9892014. Source: ProtInc peristalsis Inferred from electronic annotation. Source: Compara regulation of muscle contraction Inferred from electronic annotation. Source: Compara response to nutrient Traceable author statement Ref.1. Source: ProtInc response to starvation Inferred from electronic annotation. Source: Compara spermatogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara integral to plasma membrane Traceable author statement PubMed 7914078. Source: ProtInc
Molecular_function	somatostatin receptor activity Traceable author statement PubMed 7914078. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SSTR3	P32745	3	EBI-6266898,EBI-6266935

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P30874-1)

Also known as: SSTR2A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P30874-2)

Also known as: SSTR2B;

The sequence of this isoform differs from the canonical sequence as follows:
     332-369: VSGTDDGERSDSKQDKSRLNETTETQRTLLNGDLQTSI → VDNSKSGEEGSCLDMIFRNNKNRKK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Somatostatin receptor type 2		PRO_0000070120
Regions
	Topological domain	1 – 43	43	Extracellular Potential
	Transmembrane	44 – 67	24	Helical; Name=1; Potential
	Topological domain	68 – 78	11	Cytoplasmic Potential
	Transmembrane	79 – 103	25	Helical; Name=2; Potential
	Topological domain	104 – 118	15	Extracellular Potential
	Transmembrane	119 – 138	20	Helical; Name=3; Potential
	Topological domain	139 – 161	23	Cytoplasmic Potential
	Transmembrane	162 – 181	20	Helical; Name=4; Potential
	Topological domain	182 – 207	26	Extracellular Potential
	Transmembrane	208 – 229	22	Helical; Name=5; Potential
	Topological domain	230 – 253	24	Cytoplasmic Potential
	Transmembrane	254 – 278	25	Helical; Name=6; Potential
	Topological domain	279 – 288	10	Extracellular Potential
	Transmembrane	289 – 303	15	Helical; Name=7; Potential
	Topological domain	304 – 369	66	Cytoplasmic Potential
Amino acid modifications
	Lipidation	328	1	S-palmitoyl cysteine Potential
	Glycosylation	9	1	N-linked (GlcNAc...) Potential
	Glycosylation	22	1	N-linked (GlcNAc...) Potential
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	32	1	N-linked (GlcNAc...) Potential
	Disulfide bond	115 ↔ 193		By similarity
Natural variations
	Alternative sequence	332 – 369	38	VSGTD…LQTSI → VDNSKSGEEGSCLDMIFRNN KNRKK in isoform B.		VSP_001922

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (SSTR2A) [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 3B5D7D8A9AC246C6	FASTA	369	41,333
	10 20 30 40 50 60 MDMADEPLNG SHTWLSIPFD LNGSVVSTNT SNQTEPYYDL TSNAVLTFIY FVVCIIGLCG 70 80 90 100 110 120 NTLVIYVILR YAKMKTITNI YILNLAIADE LFMLGLPFLA MQVALVHWPF GKAICRVVMT 130 140 150 160 170 180 VDGINQFTSI FCLTVMSIDR YLAVVHPIKS AKWRRPRTAK MITMAVWGVS LLVILPIMIY 190 200 210 220 230 240 AGLRSNQWGR SSCTINWPGE SGAWYTGFII YTFILGFLVP LTIICLCYLF IIIKVKSSGI 250 260 270 280 290 300 RVGSSKRKKS EKKVTRMVSI VVAVFIFCWL PFYIFNVSSV SMAISPTPAL KGMFDFVVVL 310 320 330 340 350 360 TYANSCANPI LYAFLSDNFK KSFQNVLCLV KVSGTDDGER SDSKQDKSRL NETTETQRTL LNGDLQTSI
	Isoform B (SSTR2B) [UniParc]. Checksum: D10FA237FAED61F3	FASTA	356	40,007
	10 20 30 40 50 60 MDMADEPLNG SHTWLSIPFD LNGSVVSTNT SNQTEPYYDL TSNAVLTFIY FVVCIIGLCG 70 80 90 100 110 120 NTLVIYVILR YAKMKTITNI YILNLAIADE LFMLGLPFLA MQVALVHWPF GKAICRVVMT 130 140 150 160 170 180 VDGINQFTSI FCLTVMSIDR YLAVVHPIKS AKWRRPRTAK MITMAVWGVS LLVILPIMIY 190 200 210 220 230 240 AGLRSNQWGR SSCTINWPGE SGAWYTGFII YTFILGFLVP LTIICLCYLF IIIKVKSSGI 250 260 270 280 290 300 RVGSSKRKKS EKKVTRMVSI VVAVFIFCWL PFYIFNVSSV SMAISPTPAL KGMFDFVVVL 310 320 330 340 350 TYANSCANPI LYAFLSDNFK KSFQNVLCLV KVDNSKSGEE GSCLDMIFRN NKNRKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and functional characterization of a family of human and mouse somatostatin receptors expressed in brain, gastrointestinal tract, and kidney." Yamada Y., Post S.R., Wang K., Tager H.S., Bell G.I., Seino S. Proc. Natl. Acad. Sci. U.S.A. 89:251-255(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
[2]	"Genomic structure and transcriptional regulation of the human somatostatin receptor type 2." Petersenn S., Rasch A.C., Presch S., Beil F.U., Schulte H.M. Mol. Cell. Endocrinol. 157:75-85(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS A AND B).
[3]	"Isolation of complete coding sequence for somatostatin receptor 2 (SSTR2)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes." Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
[6]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Tissue: Eye.
[8]	"Multiple gene transcripts of the somatostatin receptor SSTR2: tissue selective distribution and cAMP regulation." Patel Y.C., Greenwood M., Kent G., Panetta R., Srikant C.B. Biochem. Biophys. Res. Commun. 192:288-294(1993) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING.
[9]	"Somatostatin receptor interacting protein defines a novel family of multidomain proteins present in human and rodent brain." Zitzer H., Hoenck H.-H., Baechner D., Richter D., Kreienkamp H.-J. J. Biol. Chem. 274:32997-33001(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SHANK1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M81830 Genomic DNA. Translation: AAA58248.1. AF184174 Genomic DNA. Translation: AAF42809.1. AF184174 Genomic DNA. Translation: AAF42810.1. AB065911 Genomic DNA. Translation: BAC06126.1. AY236542 Genomic DNA. Translation: AAO92064.1. AK290745 mRNA. Translation: BAF83434.1. BT019926 mRNA. Translation: AAV38729.1. BC000256 mRNA. Translation: AAH00256.1. BC009522 mRNA. Translation: AAH09522.1. BC019610 mRNA. Translation: AAH19610.1. BC095495 mRNA. Translation: AAH95495.1.
IPI	IPI00027807. IPI00046027.
PIR	B41795.
RefSeq	NP_001041.1. NM_001050.2.
UniGene	Hs.514451.
3D structure databases
ProteinModelPortal	P30874.
ModBase	Search...
Protein-protein interaction databases
IntAct	P30874. 1 interaction.
MINT	MINT-3340293.
STRING	9606.ENSP00000350198.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P30874.
Polymorphism databases
DMDM	401126.
Proteomic databases
PaxDb	P30874.
PRIDE	P30874.
Protocols and materials databases
DNASU	6752.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000315332; ENSP00000326616; ENSG00000180616. ENST00000357585; ENSP00000350198; ENSG00000180616.
GeneID	6752.
KEGG	hsa:6752.
UCSC	uc002jje.3. human.
Organism-specific databases
CTD	6752.
GeneCards	GC17P071161.
HGNC	HGNC:11331. SSTR2.
HPA	HPA007264.
MIM	182452. gene.
neXtProt	NX_P30874.
PharmGKB	PA36155.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG274661.
HOGENOM	HOG000230485.
HOVERGEN	HBG106919.
InParanoid	P30874.
KO	K04218.
OMA	STVVITC.
OrthoDB	EOG4PK283.
PhylomeDB	P30874.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P30874.
CleanEx	HS_SSTR2.
Genevestigator	P30874.
GermOnline	ENSG00000180616. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000586. Somatstn_rcpt. IPR002074. Somatstn_rcpt_2. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00246. SOMATOSTATNR. PR00588. SOMATOSTTN2R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P30874.
ChEMBL	CHEMBL1804.
GenomeRNAi	6752.
NextBio	26340.
SOURCE	Search...

Entry information

Entry name

SSR2_HUMAN

Accession

Primary (citable) accession number: P30874
Secondary accession number(s): A8K3Y0 , Q4VBP0, Q96GE0, Q96TF2, Q9BWH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.